8TX5
Crystal structure of an engineered variant of galactose oxidase, GOaseRd4BB, from Fusarium graminearum
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0045480 | molecular_function | galactose oxidase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0045480 | molecular_function | galactose oxidase activity |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0005576 | cellular_component | extracellular region |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0045480 | molecular_function | galactose oxidase activity |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0005576 | cellular_component | extracellular region |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0045480 | molecular_function | galactose oxidase activity |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DGNQNGWIGrhEV |
| Chain | Residue | Details |
| A | ASP75-VAL87 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 588 |
| Details | Domain: {"description":"F5/8 type C","evidences":[{"source":"PROSITE-ProRule","id":"PRU00081","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 180 |
| Details | Repeat: {"description":"Kelch 1"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 168 |
| Details | Repeat: {"description":"Kelch 2"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 196 |
| Details | Repeat: {"description":"Kelch 3"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 216 |
| Details | Repeat: {"description":"Kelch 4"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 208 |
| Details | Repeat: {"description":"Kelch 5"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"1997","firstPage":"327","lastPage":"335","volume":"2","journal":"J. Biol. Inorg. Chem.","title":"Structure and mechanism of galactose oxidase: catalytic role of tyrosine 495.","authors":["Reynolds M.P.","Baron A.J.","Wilmot C.M.","Vinecombe E.","Stevens C.","Phillips S.E.V.","Knowles P.F.","McPherson M.J."],"citationCrossReferences":[{"database":"DOI","id":"10.1007/s007750050139"}]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 16 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 8 |
| Details | Cross-link: {"description":"3'-(S-cysteinyl)-tyrosine (Cys-Tyr)"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| A | CYS228 | activator, covalently attached, metal ligand |
| A | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| A | PHE290 | activator, radical stabiliser |
| A | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| A | HIS496 | metal ligand |
| A | HIS581 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| B | CYS228 | activator, covalently attached, metal ligand |
| B | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| B | PHE290 | activator, radical stabiliser |
| B | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| B | HIS496 | metal ligand |
| B | HIS581 | metal ligand |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| C | CYS228 | activator, covalently attached, metal ligand |
| C | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| C | PHE290 | activator, radical stabiliser |
| C | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| C | HIS496 | metal ligand |
| C | HIS581 | metal ligand |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| D | CYS228 | activator, covalently attached, metal ligand |
| D | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| D | PHE290 | activator, radical stabiliser |
| D | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| D | HIS496 | metal ligand |
| D | HIS581 | metal ligand |
