8TX5
Crystal structure of an engineered variant of galactose oxidase, GOaseRd4BB, from Fusarium graminearum
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DGNQNGWIGrhEV |
| Chain | Residue | Details |
| A | ASP75-VAL87 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|DOI:10.1007/s007750050139 |
| Chain | Residue | Details |
| A | TYR495 | |
| B | TYR495 | |
| C | TYR495 | |
| D | TYR495 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | TYR272 | |
| C | TYR495 | |
| C | HIS496 | |
| C | HIS581 | |
| D | TYR272 | |
| D | TYR495 | |
| D | HIS496 | |
| D | HIS581 | |
| A | TYR495 | |
| A | HIS496 | |
| A | HIS581 | |
| B | TYR272 | |
| B | TYR495 | |
| B | HIS496 | |
| B | HIS581 | |
| C | TYR272 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | CROSSLNK: 3'-(S-cysteinyl)-tyrosine (Cys-Tyr) |
| Chain | Residue | Details |
| A | CYS228 | |
| A | TYR272 | |
| B | CYS228 | |
| B | TYR272 | |
| C | CYS228 | |
| C | TYR272 | |
| D | CYS228 | |
| D | TYR272 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| A | CYS228 | activator, covalently attached, metal ligand |
| A | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| A | PHE290 | activator, radical stabiliser |
| A | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| A | HIS496 | metal ligand |
| A | HIS581 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| B | CYS228 | activator, covalently attached, metal ligand |
| B | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| B | PHE290 | activator, radical stabiliser |
| B | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| B | HIS496 | metal ligand |
| B | HIS581 | metal ligand |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| C | CYS228 | activator, covalently attached, metal ligand |
| C | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| C | PHE290 | activator, radical stabiliser |
| C | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| C | HIS496 | metal ligand |
| C | HIS581 | metal ligand |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 322 |
| Chain | Residue | Details |
| D | CYS228 | activator, covalently attached, metal ligand |
| D | TYR272 | activator, hydrogen radical acceptor, hydrogen radical donor, metal ligand |
| D | PHE290 | activator, radical stabiliser |
| D | TYR495 | activator, metal ligand, proton acceptor, proton donor |
| D | HIS496 | metal ligand |
| D | HIS581 | metal ligand |
