8TX5
Crystal structure of an engineered variant of galactose oxidase, GOaseRd4BB, from Fusarium graminearum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.010, 205.753, 89.291 |
| Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
| Resolution | 28.020 - 1.930 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.408 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.020 | 2.190 |
| High resolution limit [Å] | 1.930 | 2.080 |
| Number of reflections | 223522 | 27814 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 92.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.996 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% vol PEG 3350, 200 mM Potassium monophosphate, 100 mM TRIS.HCl (pH 8.0) |
