Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8TBU

Structure of human erythrocyte pyruvate kinase in complex with an allosteric activator Compound 12

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001666	biological_process	response to hypoxia
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006096	biological_process	glycolytic process
A	0007584	biological_process	response to nutrient
A	0009749	biological_process	response to glucose
A	0010038	biological_process	response to metal ion
A	0016301	molecular_function	kinase activity
A	0030955	molecular_function	potassium ion binding
A	0032869	biological_process	cellular response to insulin stimulus
A	0033198	biological_process	response to ATP
A	0042866	biological_process	pyruvate biosynthetic process
A	0046872	molecular_function	metal ion binding
A	0048029	molecular_function	monosaccharide binding
A	0051591	biological_process	response to cAMP
A	0070062	cellular_component	extracellular exosome
A	0071872	biological_process	cellular response to epinephrine stimulus
B	0000287	molecular_function	magnesium ion binding
B	0001666	biological_process	response to hypoxia
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006096	biological_process	glycolytic process
B	0007584	biological_process	response to nutrient
B	0009749	biological_process	response to glucose
B	0010038	biological_process	response to metal ion
B	0016301	molecular_function	kinase activity
B	0030955	molecular_function	potassium ion binding
B	0032869	biological_process	cellular response to insulin stimulus
B	0033198	biological_process	response to ATP
B	0042866	biological_process	pyruvate biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0048029	molecular_function	monosaccharide binding
B	0051591	biological_process	response to cAMP
B	0070062	cellular_component	extracellular exosome
B	0071872	biological_process	cellular response to epinephrine stimulus
C	0000287	molecular_function	magnesium ion binding
C	0001666	biological_process	response to hypoxia
C	0003824	molecular_function	catalytic activity
C	0004743	molecular_function	pyruvate kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006096	biological_process	glycolytic process
C	0007584	biological_process	response to nutrient
C	0009749	biological_process	response to glucose
C	0010038	biological_process	response to metal ion
C	0016301	molecular_function	kinase activity
C	0030955	molecular_function	potassium ion binding
C	0032869	biological_process	cellular response to insulin stimulus
C	0033198	biological_process	response to ATP
C	0042866	biological_process	pyruvate biosynthetic process
C	0046872	molecular_function	metal ion binding
C	0048029	molecular_function	monosaccharide binding
C	0051591	biological_process	response to cAMP
C	0070062	cellular_component	extracellular exosome
C	0071872	biological_process	cellular response to epinephrine stimulus
D	0000287	molecular_function	magnesium ion binding
D	0001666	biological_process	response to hypoxia
D	0003824	molecular_function	catalytic activity
D	0004743	molecular_function	pyruvate kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006096	biological_process	glycolytic process
D	0007584	biological_process	response to nutrient
D	0009749	biological_process	response to glucose
D	0010038	biological_process	response to metal ion
D	0016301	molecular_function	kinase activity
D	0030955	molecular_function	potassium ion binding
D	0032869	biological_process	cellular response to insulin stimulus
D	0033198	biological_process	response to ATP
D	0042866	biological_process	pyruvate biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0048029	molecular_function	monosaccharide binding
D	0051591	biological_process	response to cAMP
D	0070062	cellular_component	extracellular exosome
D	0071872	biological_process	cellular response to epinephrine stimulus
E	0000287	molecular_function	magnesium ion binding
E	0001666	biological_process	response to hypoxia
E	0003824	molecular_function	catalytic activity
E	0004743	molecular_function	pyruvate kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006096	biological_process	glycolytic process
E	0007584	biological_process	response to nutrient
E	0009749	biological_process	response to glucose
E	0010038	biological_process	response to metal ion
E	0016301	molecular_function	kinase activity
E	0030955	molecular_function	potassium ion binding
E	0032869	biological_process	cellular response to insulin stimulus
E	0033198	biological_process	response to ATP
E	0042866	biological_process	pyruvate biosynthetic process
E	0046872	molecular_function	metal ion binding
E	0048029	molecular_function	monosaccharide binding
E	0051591	biological_process	response to cAMP
E	0070062	cellular_component	extracellular exosome
E	0071872	biological_process	cellular response to epinephrine stimulus
F	0000287	molecular_function	magnesium ion binding
F	0001666	biological_process	response to hypoxia
F	0003824	molecular_function	catalytic activity
F	0004743	molecular_function	pyruvate kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006096	biological_process	glycolytic process
F	0007584	biological_process	response to nutrient
F	0009749	biological_process	response to glucose
F	0010038	biological_process	response to metal ion
F	0016301	molecular_function	kinase activity
F	0030955	molecular_function	potassium ion binding
F	0032869	biological_process	cellular response to insulin stimulus
F	0033198	biological_process	response to ATP
F	0042866	biological_process	pyruvate biosynthetic process
F	0046872	molecular_function	metal ion binding
F	0048029	molecular_function	monosaccharide binding
F	0051591	biological_process	response to cAMP
F	0070062	cellular_component	extracellular exosome
F	0071872	biological_process	cellular response to epinephrine stimulus
G	0000287	molecular_function	magnesium ion binding
G	0001666	biological_process	response to hypoxia
G	0003824	molecular_function	catalytic activity
G	0004743	molecular_function	pyruvate kinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006096	biological_process	glycolytic process
G	0007584	biological_process	response to nutrient
G	0009749	biological_process	response to glucose
G	0010038	biological_process	response to metal ion
G	0016301	molecular_function	kinase activity
G	0030955	molecular_function	potassium ion binding
G	0032869	biological_process	cellular response to insulin stimulus
G	0033198	biological_process	response to ATP
G	0042866	biological_process	pyruvate biosynthetic process
G	0046872	molecular_function	metal ion binding
G	0048029	molecular_function	monosaccharide binding
G	0051591	biological_process	response to cAMP
G	0070062	cellular_component	extracellular exosome
G	0071872	biological_process	cellular response to epinephrine stimulus
H	0000287	molecular_function	magnesium ion binding
H	0001666	biological_process	response to hypoxia
H	0003824	molecular_function	catalytic activity
H	0004743	molecular_function	pyruvate kinase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006096	biological_process	glycolytic process
H	0007584	biological_process	response to nutrient
H	0009749	biological_process	response to glucose
H	0010038	biological_process	response to metal ion
H	0016301	molecular_function	kinase activity
H	0030955	molecular_function	potassium ion binding
H	0032869	biological_process	cellular response to insulin stimulus
H	0033198	biological_process	response to ATP
H	0042866	biological_process	pyruvate biosynthetic process
H	0046872	molecular_function	metal ion binding
H	0048029	molecular_function	monosaccharide binding
H	0051591	biological_process	response to cAMP
H	0070062	cellular_component	extracellular exosome
H	0071872	biological_process	cellular response to epinephrine stimulus

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. IkIISKIENhEGV

Chain	Residue	Details
A	ILE308-VAL320

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGB

Chain	Residue	Details
A	ARG116
E	SER120
F	ARG116
F	SER120
G	ARG116
G	SER120
H	ARG116
H	SER120
A	SER120
B	ARG116
B	SER120
C	ARG116
C	SER120
D	ARG116
D	SER120
E	ARG116

site_id	SWS_FT_FI2
Number of Residues	96
Details	BINDING: BINDING => ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGF

Chain	Residue	Details
A	ASN118
A	TRP525
A	ARG532
A	ARG559
B	ASN118
B	ASP156
B	THR157
B	LYS313
B	GLU315
B	GLY338
B	ASP339
A	ASP156
B	THR371
B	THR475
B	TRP525
B	ARG532
B	ARG559
C	ASN118
C	ASP156
C	THR157
C	LYS313
C	GLU315
A	THR157
C	GLY338
C	ASP339
C	THR371
C	THR475
C	TRP525
C	ARG532
C	ARG559
D	ASN118
D	ASP156
D	THR157
A	LYS313
D	LYS313
D	GLU315
D	GLY338
D	ASP339
D	THR371
D	THR475
D	TRP525
D	ARG532
D	ARG559
E	ASN118
A	GLU315
E	ASP156
E	THR157
E	LYS313
E	GLU315
E	GLY338
E	ASP339
E	THR371
E	THR475
E	TRP525
E	ARG532
A	GLY338
E	ARG559
F	ASN118
F	ASP156
F	THR157
F	LYS313
F	GLU315
F	GLY338
F	ASP339
F	THR371
F	THR475
A	ASP339
F	TRP525
F	ARG532
F	ARG559
G	ASN118
G	ASP156
G	THR157
G	LYS313
G	GLU315
G	GLY338
G	ASP339
A	THR371
G	THR371
G	THR475
G	TRP525
G	ARG532
G	ARG559
H	ASN118
H	ASP156
H	THR157
H	LYS313
H	GLU315
A	THR475
H	GLY338
H	ASP339
H	THR371
H	THR475
H	TRP525
H	ARG532
H	ARG559

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P14618

Chain	Residue	Details
A	ARG163
E	LYS250
F	ARG163
F	LYS250
G	ARG163
G	LYS250
H	ARG163
H	LYS250
A	LYS250
B	ARG163
B	LYS250
C	ARG163
C	LYS250
D	ARG163
D	LYS250
E	ARG163

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Transition state stabilizer => ECO:0000250\|UniProtKB:P00549

Chain	Residue	Details
A	LYS313
B	LYS313
C	LYS313
D	LYS313
E	LYS313
F	LYS313
G	LYS313
H	LYS313

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
A	SER292
B	SER292
C	SER292
D	SER292
E	SER292
F	SER292
G	SER292
H	SER292

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)