8SZG

Cryo-EM structure of cinacalcet-bound human calcium-sensing receptor CaSR-Gq complex in lipid nanodiscs

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004930	molecular_function	G protein-coupled receptor activity
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0016020	cellular_component	membrane
B	0004930	molecular_function	G protein-coupled receptor activity
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0016020	cellular_component	membrane
C	0000166	molecular_function	nucleotide binding
C	0001664	molecular_function	G protein-coupled receptor binding
C	0001750	cellular_component	photoreceptor outer segment
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005096	molecular_function	GTPase activator activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005794	cellular_component	Golgi apparatus
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
C	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
C	0007206	biological_process	phospholipase C-activating G protein-coupled glutamate receptor signaling pathway
C	0007208	biological_process	phospholipase C-activating serotonin receptor signaling pathway
C	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
C	0007215	biological_process	glutamate receptor signaling pathway
C	0007218	biological_process	neuropeptide signaling pathway
C	0007596	biological_process	blood coagulation
C	0007603	biological_process	phototransduction, visible light
C	0009649	biological_process	entrainment of circadian clock
C	0009755	biological_process	hormone-mediated signaling pathway
C	0010543	biological_process	regulation of platelet activation
C	0016020	cellular_component	membrane
C	0016787	molecular_function	hydrolase activity
C	0019001	molecular_function	guanyl nucleotide binding
C	0030234	molecular_function	enzyme regulator activity
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0031965	cellular_component	nuclear membrane
C	0034695	biological_process	response to prostaglandin E
C	0046872	molecular_function	metal ion binding
C	0050821	biological_process	protein stabilization
C	0060828	biological_process	regulation of canonical Wnt signaling pathway
C	0070062	cellular_component	extracellular exosome
C	0071468	biological_process	cellular response to acidic pH
C	0099524	cellular_component	postsynaptic cytosol
C	0140582	biological_process	adenylate cyclase-activating G protein-coupled cAMP receptor signaling pathway
D	0001750	cellular_component	photoreceptor outer segment
D	0003924	molecular_function	GTPase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005765	cellular_component	lysosomal membrane
D	0005829	cellular_component	cytosol
D	0005834	cellular_component	heterotrimeric G-protein complex
D	0005886	cellular_component	plasma membrane
D	0007165	biological_process	signal transduction
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
D	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
D	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
D	0007265	biological_process	Ras protein signal transduction
D	0008283	biological_process	cell population proliferation
D	0016020	cellular_component	membrane
D	0030159	molecular_function	signaling receptor complex adaptor activity
D	0044877	molecular_function	protein-containing complex binding
D	0045202	cellular_component	synapse
D	0050909	biological_process	sensory perception of taste
D	0051020	molecular_function	GTPase binding
D	0060041	biological_process	retina development in camera-type eye
D	0070062	cellular_component	extracellular exosome
D	0071380	biological_process	cellular response to prostaglandin E stimulus
D	0071870	biological_process	cellular response to catecholamine stimulus
D	0097381	cellular_component	photoreceptor disc membrane
D	1903561	cellular_component	extracellular vesicle
E	0005515	molecular_function	protein binding
E	0005834	cellular_component	heterotrimeric G-protein complex
E	0005886	cellular_component	plasma membrane
E	0007165	biological_process	signal transduction
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
E	0016020	cellular_component	membrane
E	0031681	molecular_function	G-protein beta-subunit binding
E	0045202	cellular_component	synapse
E	0048144	biological_process	fibroblast proliferation
E	0070062	cellular_component	extracellular exosome
E	0071380	biological_process	cellular response to prostaglandin E stimulus
E	0071870	biological_process	cellular response to catecholamine stimulus

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
D	LEU70-SER84
D	ILE157-ILE171
D	LEU285-ALA299
D	VAL327-GLY341

---

site_id	PS00979
Number of Residues	19
Details	G_PROTEIN_RECEP_F3_1 G-protein coupled receptors family 3 signature 1. VaNLLgLFyIPQVSyASSS

Chain	Residue	Details
B	VAL153-SER171
A	VAL153-SER171

---

site_id	PS00980
Number of Residues	25
Details	G_PROTEIN_RECEP_F3_2 G-protein coupled receptors family 3 signature 2. CCFeCveCpdgeYsdet.DasACnkC

Chain	Residue	Details
B	CYS561-CYS585
A	CYS561-CYS585

---

site_id	PS00981
Number of Residues	11
Details	G_PROTEIN_RECEP_F3_3 G-protein coupled receptors family 3 signature 3. FNEAKfITFSM

Chain	Residue	Details
B	PHE801-MET811
A	PHE801-MET811

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P21279

Chain	Residue	Details
C	SER50
C	THR186
C	ASN274
C	LYS275
C	ASP277
C	ALA331
C	GLY51
C	LYS52
C	SER53
C	THR54
C	SER156
C	LEU180
C	ARG181
C	ARG183

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Deamidated glutamine; by Photorhabdus PAU_02230 => ECO:0000269|PubMed:24141704

Chain	Residue	Details
C	GLN209

---

site_id	SWS_FT_FI3
Number of Residues	48
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
B	LYS636-GLU649
B	ARG701-GLN724
B	LYS793-LYS805

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	TRANSMEM: Helical; Name=2 => ECO:0000255

Chain	Residue	Details
B	LEU650-GLY670

---

site_id	SWS_FT_FI5
Number of Residues	18
Details	TRANSMEM: Helical; Name=3 => ECO:0000255

Chain	Residue	Details
B	PRO682-ASN700

---

site_id	SWS_FT_FI6
Number of Residues	20
Details	TRANSMEM: Helical; Name=4 => ECO:0000255

Chain	Residue	Details
B	PHE725-THR745

---

site_id	SWS_FT_FI7
Number of Residues	22
Details	TRANSMEM: Helical; Name=5 => ECO:0000255

Chain	Residue	Details
B	LEU770-PHE792

---

site_id	SWS_FT_FI8
Number of Residues	22
Details	TRANSMEM: Helical; Name=6 => ECO:0000255

Chain	Residue	Details
B	PHE806-THR828

---

site_id	SWS_FT_FI9
Number of Residues	25
Details	TRANSMEM: Helical; Name=7 => ECO:0000255

Chain	Residue	Details
B	GLU837-PHE862

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5K5S

Chain	Residue	Details
B	ARG66
B	THR145
B	GLU231
B	ASP234
B	ARG415
B	GLY557

---

site_id	SWS_FT_FI11
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0000305|PubMed:27386547, ECO:0007744|PDB:5FBH, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S

Chain	Residue	Details
B	ILE81
B	LEU87
B	LEU88

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0000305|PubMed:27386547, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S

Chain	Residue	Details
B	SER84

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5K5S, ECO:0007744|PDB:5K5T

Chain	Residue	Details
B	THR100

---

site_id	SWS_FT_FI14
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:27386547, ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5FBH, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S

Chain	Residue	Details
B	SER147
B	ALA168
B	SER170
B	GLU297

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	SITE: Important for ability of agonist AMG 416 to activate G-protein-coupled receptor activity => ECO:0000269|PubMed:26290606

Chain	Residue	Details
B	CYS482

---

site_id	SWS_FT_FI16
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
B	ASN90
B	ASN130
B	ASN386
B	ASN400

---

site_id	SWS_FT_FI17
Number of Residues	7
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255, ECO:0000269|PubMed:27434672

Chain	Residue	Details
B	ASN261
B	ASN287
B	ASN446
B	ASN468
B	ASN488
B	ASN541
B	ASN594

---