8SFR
WT CRISPR-Cas12a post nontarget strand cleavage.
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0003677 | molecular_function | DNA binding |
A | 0003723 | molecular_function | RNA binding |
A | 0004519 | molecular_function | endonuclease activity |
A | 0004530 | molecular_function | deoxyribonuclease I activity |
A | 0006259 | biological_process | DNA metabolic process |
A | 0016829 | molecular_function | lyase activity |
A | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 8 |
Details | DNA_BIND: PAM-binding on target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | PRO599-LYS607 |
site_id | SWS_FT_FI2 |
Number of Residues | 22 |
Details | DNA_BIND: Target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | LYS780-GLY783 | |
A | ARG951-LYS968 | |
A | SER1051-ALA1053 |
site_id | SWS_FT_FI3 |
Number of Residues | 3 |
Details | ACT_SITE: For pre-crRNA processing => ECO:0000250|UniProtKB:A0Q7Q2 |
Chain | Residue | Details |
A | HIS800 | |
A | LYS809 | |
A | LYS860 |
site_id | SWS_FT_FI4 |
Number of Residues | 3 |
Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000269|PubMed:27114038 |
Chain | Residue | Details |
A | ASP908 | |
A | GLU993 | |
A | ASP1263 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | ACT_SITE: For DNase activity of nuclease domain => ECO:0000269|PubMed:27114038 |
Chain | Residue | Details |
A | ARG1226 |
site_id | SWS_FT_FI6 |
Number of Residues | 4 |
Details | BINDING: BINDING => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | TYR47 | |
A | ASN175 | |
A | LYS307 | |
A | LYS752 |
site_id | SWS_FT_FI7 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:27114038 |
Chain | Residue | Details |
A | MET806 |
site_id | SWS_FT_FI8 |
Number of Residues | 3 |
Details | SITE: Binds crRNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | ARG18 | |
A | ARG192 | |
A | HIS872 |
site_id | SWS_FT_FI9 |
Number of Residues | 2 |
Details | SITE: Binds PAM on target DNA => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | THR167 | |
A | LYS548 |
site_id | SWS_FT_FI10 |
Number of Residues | 1 |
Details | SITE: Binds crRNA-target DNA heteroduplex => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | TRP382 |
site_id | SWS_FT_FI11 |
Number of Residues | 1 |
Details | SITE: Binds sequence-specific recognition of both target and non-target strand bases in PAM => ECO:0000269|PubMed:27114038, ECO:0000269|PubMed:27444870 |
Chain | Residue | Details |
A | LYS607 |
site_id | SWS_FT_FI12 |
Number of Residues | 1 |
Details | SITE: Binds target DNA => ECO:0000269|PubMed:27114038 |
Chain | Residue | Details |
A | GLN1014 |