8SFR
WT CRISPR-Cas12a post nontarget strand cleavage.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Software | cryoSPARC,SerialEM |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |