8SFQ
WT CRISPR-Cas12a post nontarget strand-cleavage with the the RuvC active site exposed.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | DNA binding: {"description":"PAM-binding on target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 22 |
| Details | DNA binding: {"description":"Target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 34 |
| Details | Region: {"description":"WED-I (OBD-I)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 284 |
| Details | Region: {"description":"REC1 (helical-I)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 71 |
| Details | Region: {"description":"WED-II (OBD-I)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 119 |
| Details | Region: {"description":"PI (LHD)","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 55 |
| Details | Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 16 |
| Details | Region: {"description":"Bridge helix","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 108 |
| Details | Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 195 |
| Details | Region: {"description":"Nuclease domain","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 44 |
| Details | Region: {"description":"RuvC-III","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000303"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 32 |
| Details | Coiled coil: {"evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Active site: {"description":"For pre-crRNA processing","evidences":[{"source":"UniProtKB","id":"A0Q7Q2","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 3 |
| Details | Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | Active site: {"description":"For DNase activity of nuclease domain","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 17 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 3 |
| Details | Site: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds PAM on target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds sequence-specific recognition of both target and non-target strand bases in PAM","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27444870","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds target DNA","evidences":[{"source":"PubMed","id":"27114038","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
