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8SFQ

WT CRISPR-Cas12a post nontarget strand-cleavage with the the RuvC active site exposed.

EM specimen
VitrificationYes
InstrumentFEI VITROBOT MARK IV
EM Data Collection
MicroscopeTFS KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
DetectorGATAN K3 (6k x 4k)
3D Reconstruction
MethodSINGLE PARTICLE
Resolution (Å)3.5
SoftwarecryoSPARC,SerialEM
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION

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PDB entries from 2024-07-24

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