8S88

Crystal structure of human L-lactate Dehydrogenase B protein in complex with NADH, oxamate and fluoxetine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004457	molecular_function	lactate dehydrogenase activity
A	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005829	cellular_component	cytosol
A	0006089	biological_process	lactate metabolic process
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019674	biological_process	NAD+ metabolic process
A	0019752	biological_process	carboxylic acid metabolic process
A	0019900	molecular_function	kinase binding
A	0042802	molecular_function	identical protein binding
A	0042867	biological_process	pyruvate catabolic process
A	0045121	cellular_component	membrane raft
A	0051287	molecular_function	NAD binding
A	0070062	cellular_component	extracellular exosome
A	1990204	cellular_component	oxidoreductase complex
B	0003824	molecular_function	catalytic activity
B	0004457	molecular_function	lactate dehydrogenase activity
B	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005829	cellular_component	cytosol
B	0006089	biological_process	lactate metabolic process
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019674	biological_process	NAD+ metabolic process
B	0019752	biological_process	carboxylic acid metabolic process
B	0019900	molecular_function	kinase binding
B	0042802	molecular_function	identical protein binding
B	0042867	biological_process	pyruvate catabolic process
B	0045121	cellular_component	membrane raft
B	0051287	molecular_function	NAD binding
B	0070062	cellular_component	extracellular exosome
B	1990204	cellular_component	oxidoreductase complex
C	0003824	molecular_function	catalytic activity
C	0004457	molecular_function	lactate dehydrogenase activity
C	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005829	cellular_component	cytosol
C	0006089	biological_process	lactate metabolic process
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019674	biological_process	NAD+ metabolic process
C	0019752	biological_process	carboxylic acid metabolic process
C	0019900	molecular_function	kinase binding
C	0042802	molecular_function	identical protein binding
C	0042867	biological_process	pyruvate catabolic process
C	0045121	cellular_component	membrane raft
C	0051287	molecular_function	NAD binding
C	0070062	cellular_component	extracellular exosome
C	1990204	cellular_component	oxidoreductase complex
D	0003824	molecular_function	catalytic activity
D	0004457	molecular_function	lactate dehydrogenase activity
D	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005829	cellular_component	cytosol
D	0006089	biological_process	lactate metabolic process
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019674	biological_process	NAD+ metabolic process
D	0019752	biological_process	carboxylic acid metabolic process
D	0019900	molecular_function	kinase binding
D	0042802	molecular_function	identical protein binding
D	0042867	biological_process	pyruvate catabolic process
D	0045121	cellular_component	membrane raft
D	0051287	molecular_function	NAD binding
D	0070062	cellular_component	extracellular exosome
D	1990204	cellular_component	oxidoreductase complex
E	0003824	molecular_function	catalytic activity
E	0004457	molecular_function	lactate dehydrogenase activity
E	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005829	cellular_component	cytosol
E	0006089	biological_process	lactate metabolic process
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0019674	biological_process	NAD+ metabolic process
E	0019752	biological_process	carboxylic acid metabolic process
E	0019900	molecular_function	kinase binding
E	0042802	molecular_function	identical protein binding
E	0042867	biological_process	pyruvate catabolic process
E	0045121	cellular_component	membrane raft
E	0051287	molecular_function	NAD binding
E	0070062	cellular_component	extracellular exosome
E	1990204	cellular_component	oxidoreductase complex
F	0003824	molecular_function	catalytic activity
F	0004457	molecular_function	lactate dehydrogenase activity
F	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005829	cellular_component	cytosol
F	0006089	biological_process	lactate metabolic process
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0019674	biological_process	NAD+ metabolic process
F	0019752	biological_process	carboxylic acid metabolic process
F	0019900	molecular_function	kinase binding
F	0042802	molecular_function	identical protein binding
F	0042867	biological_process	pyruvate catabolic process
F	0045121	cellular_component	membrane raft
F	0051287	molecular_function	NAD binding
F	0070062	cellular_component	extracellular exosome
F	1990204	cellular_component	oxidoreductase complex
G	0003824	molecular_function	catalytic activity
G	0004457	molecular_function	lactate dehydrogenase activity
G	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005829	cellular_component	cytosol
G	0006089	biological_process	lactate metabolic process
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0019674	biological_process	NAD+ metabolic process
G	0019752	biological_process	carboxylic acid metabolic process
G	0019900	molecular_function	kinase binding
G	0042802	molecular_function	identical protein binding
G	0042867	biological_process	pyruvate catabolic process
G	0045121	cellular_component	membrane raft
G	0051287	molecular_function	NAD binding
G	0070062	cellular_component	extracellular exosome
G	1990204	cellular_component	oxidoreductase complex
H	0003824	molecular_function	catalytic activity
H	0004457	molecular_function	lactate dehydrogenase activity
H	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0005829	cellular_component	cytosol
H	0006089	biological_process	lactate metabolic process
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0019674	biological_process	NAD+ metabolic process
H	0019752	biological_process	carboxylic acid metabolic process
H	0019900	molecular_function	kinase binding
H	0042802	molecular_function	identical protein binding
H	0042867	biological_process	pyruvate catabolic process
H	0045121	cellular_component	membrane raft
H	0051287	molecular_function	NAD binding
H	0070062	cellular_component	extracellular exosome
H	1990204	cellular_component	oxidoreductase complex
I	0003824	molecular_function	catalytic activity
I	0004457	molecular_function	lactate dehydrogenase activity
I	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005739	cellular_component	mitochondrion
I	0005743	cellular_component	mitochondrial inner membrane
I	0005829	cellular_component	cytosol
I	0006089	biological_process	lactate metabolic process
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
I	0019674	biological_process	NAD+ metabolic process
I	0019752	biological_process	carboxylic acid metabolic process
I	0019900	molecular_function	kinase binding
I	0042802	molecular_function	identical protein binding
I	0042867	biological_process	pyruvate catabolic process
I	0045121	cellular_component	membrane raft
I	0051287	molecular_function	NAD binding
I	0070062	cellular_component	extracellular exosome
I	1990204	cellular_component	oxidoreductase complex
J	0003824	molecular_function	catalytic activity
J	0004457	molecular_function	lactate dehydrogenase activity
J	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0005829	cellular_component	cytosol
J	0006089	biological_process	lactate metabolic process
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
J	0019674	biological_process	NAD+ metabolic process
J	0019752	biological_process	carboxylic acid metabolic process
J	0019900	molecular_function	kinase binding
J	0042802	molecular_function	identical protein binding
J	0042867	biological_process	pyruvate catabolic process
J	0045121	cellular_component	membrane raft
J	0051287	molecular_function	NAD binding
J	0070062	cellular_component	extracellular exosome
J	1990204	cellular_component	oxidoreductase complex
K	0003824	molecular_function	catalytic activity
K	0004457	molecular_function	lactate dehydrogenase activity
K	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005739	cellular_component	mitochondrion
K	0005743	cellular_component	mitochondrial inner membrane
K	0005829	cellular_component	cytosol
K	0006089	biological_process	lactate metabolic process
K	0016020	cellular_component	membrane
K	0016491	molecular_function	oxidoreductase activity
K	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
K	0019674	biological_process	NAD+ metabolic process
K	0019752	biological_process	carboxylic acid metabolic process
K	0019900	molecular_function	kinase binding
K	0042802	molecular_function	identical protein binding
K	0042867	biological_process	pyruvate catabolic process
K	0045121	cellular_component	membrane raft
K	0051287	molecular_function	NAD binding
K	0070062	cellular_component	extracellular exosome
K	1990204	cellular_component	oxidoreductase complex
L	0003824	molecular_function	catalytic activity
L	0004457	molecular_function	lactate dehydrogenase activity
L	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0005829	cellular_component	cytosol
L	0006089	biological_process	lactate metabolic process
L	0016020	cellular_component	membrane
L	0016491	molecular_function	oxidoreductase activity
L	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
L	0019674	biological_process	NAD+ metabolic process
L	0019752	biological_process	carboxylic acid metabolic process
L	0019900	molecular_function	kinase binding
L	0042802	molecular_function	identical protein binding
L	0042867	biological_process	pyruvate catabolic process
L	0045121	cellular_component	membrane raft
L	0051287	molecular_function	NAD binding
L	0070062	cellular_component	extracellular exosome
L	1990204	cellular_component	oxidoreductase complex

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU190-SER196

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	264
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11276087","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	48
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	11
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAY-2005","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	48
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	12
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---