Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8S88

Crystal structure of human L-lactate Dehydrogenase B protein in complex with NADH, oxamate and fluoxetine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004457	molecular_function	lactate dehydrogenase activity
A	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005829	cellular_component	cytosol
A	0006089	biological_process	lactate metabolic process
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0019674	biological_process	NAD+ metabolic process
A	0019900	molecular_function	kinase binding
A	0042802	molecular_function	identical protein binding
A	0042867	biological_process	pyruvate catabolic process
A	0045121	cellular_component	membrane raft
A	0051287	molecular_function	NAD binding
A	0070062	cellular_component	extracellular exosome
A	1990204	cellular_component	oxidoreductase complex
B	0004457	molecular_function	lactate dehydrogenase activity
B	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005829	cellular_component	cytosol
B	0006089	biological_process	lactate metabolic process
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0019674	biological_process	NAD+ metabolic process
B	0019900	molecular_function	kinase binding
B	0042802	molecular_function	identical protein binding
B	0042867	biological_process	pyruvate catabolic process
B	0045121	cellular_component	membrane raft
B	0051287	molecular_function	NAD binding
B	0070062	cellular_component	extracellular exosome
B	1990204	cellular_component	oxidoreductase complex
C	0004457	molecular_function	lactate dehydrogenase activity
C	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005829	cellular_component	cytosol
C	0006089	biological_process	lactate metabolic process
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0019674	biological_process	NAD+ metabolic process
C	0019900	molecular_function	kinase binding
C	0042802	molecular_function	identical protein binding
C	0042867	biological_process	pyruvate catabolic process
C	0045121	cellular_component	membrane raft
C	0051287	molecular_function	NAD binding
C	0070062	cellular_component	extracellular exosome
C	1990204	cellular_component	oxidoreductase complex
D	0004457	molecular_function	lactate dehydrogenase activity
D	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005829	cellular_component	cytosol
D	0006089	biological_process	lactate metabolic process
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0019674	biological_process	NAD+ metabolic process
D	0019900	molecular_function	kinase binding
D	0042802	molecular_function	identical protein binding
D	0042867	biological_process	pyruvate catabolic process
D	0045121	cellular_component	membrane raft
D	0051287	molecular_function	NAD binding
D	0070062	cellular_component	extracellular exosome
D	1990204	cellular_component	oxidoreductase complex
E	0004457	molecular_function	lactate dehydrogenase activity
E	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005829	cellular_component	cytosol
E	0006089	biological_process	lactate metabolic process
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0019674	biological_process	NAD+ metabolic process
E	0019900	molecular_function	kinase binding
E	0042802	molecular_function	identical protein binding
E	0042867	biological_process	pyruvate catabolic process
E	0045121	cellular_component	membrane raft
E	0051287	molecular_function	NAD binding
E	0070062	cellular_component	extracellular exosome
E	1990204	cellular_component	oxidoreductase complex
F	0004457	molecular_function	lactate dehydrogenase activity
F	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005829	cellular_component	cytosol
F	0006089	biological_process	lactate metabolic process
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0019674	biological_process	NAD+ metabolic process
F	0019900	molecular_function	kinase binding
F	0042802	molecular_function	identical protein binding
F	0042867	biological_process	pyruvate catabolic process
F	0045121	cellular_component	membrane raft
F	0051287	molecular_function	NAD binding
F	0070062	cellular_component	extracellular exosome
F	1990204	cellular_component	oxidoreductase complex
G	0004457	molecular_function	lactate dehydrogenase activity
G	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005829	cellular_component	cytosol
G	0006089	biological_process	lactate metabolic process
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0019674	biological_process	NAD+ metabolic process
G	0019900	molecular_function	kinase binding
G	0042802	molecular_function	identical protein binding
G	0042867	biological_process	pyruvate catabolic process
G	0045121	cellular_component	membrane raft
G	0051287	molecular_function	NAD binding
G	0070062	cellular_component	extracellular exosome
G	1990204	cellular_component	oxidoreductase complex
H	0004457	molecular_function	lactate dehydrogenase activity
H	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0005829	cellular_component	cytosol
H	0006089	biological_process	lactate metabolic process
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0019674	biological_process	NAD+ metabolic process
H	0019900	molecular_function	kinase binding
H	0042802	molecular_function	identical protein binding
H	0042867	biological_process	pyruvate catabolic process
H	0045121	cellular_component	membrane raft
H	0051287	molecular_function	NAD binding
H	0070062	cellular_component	extracellular exosome
H	1990204	cellular_component	oxidoreductase complex
I	0004457	molecular_function	lactate dehydrogenase activity
I	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005739	cellular_component	mitochondrion
I	0005743	cellular_component	mitochondrial inner membrane
I	0005829	cellular_component	cytosol
I	0006089	biological_process	lactate metabolic process
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0019674	biological_process	NAD+ metabolic process
I	0019900	molecular_function	kinase binding
I	0042802	molecular_function	identical protein binding
I	0042867	biological_process	pyruvate catabolic process
I	0045121	cellular_component	membrane raft
I	0051287	molecular_function	NAD binding
I	0070062	cellular_component	extracellular exosome
I	1990204	cellular_component	oxidoreductase complex
J	0004457	molecular_function	lactate dehydrogenase activity
J	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0005829	cellular_component	cytosol
J	0006089	biological_process	lactate metabolic process
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0019674	biological_process	NAD+ metabolic process
J	0019900	molecular_function	kinase binding
J	0042802	molecular_function	identical protein binding
J	0042867	biological_process	pyruvate catabolic process
J	0045121	cellular_component	membrane raft
J	0051287	molecular_function	NAD binding
J	0070062	cellular_component	extracellular exosome
J	1990204	cellular_component	oxidoreductase complex
K	0004457	molecular_function	lactate dehydrogenase activity
K	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005739	cellular_component	mitochondrion
K	0005743	cellular_component	mitochondrial inner membrane
K	0005829	cellular_component	cytosol
K	0006089	biological_process	lactate metabolic process
K	0016020	cellular_component	membrane
K	0016491	molecular_function	oxidoreductase activity
K	0019674	biological_process	NAD+ metabolic process
K	0019900	molecular_function	kinase binding
K	0042802	molecular_function	identical protein binding
K	0042867	biological_process	pyruvate catabolic process
K	0045121	cellular_component	membrane raft
K	0051287	molecular_function	NAD binding
K	0070062	cellular_component	extracellular exosome
K	1990204	cellular_component	oxidoreductase complex
L	0004457	molecular_function	lactate dehydrogenase activity
L	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0005829	cellular_component	cytosol
L	0006089	biological_process	lactate metabolic process
L	0016020	cellular_component	membrane
L	0016491	molecular_function	oxidoreductase activity
L	0019674	biological_process	NAD+ metabolic process
L	0019900	molecular_function	kinase binding
L	0042802	molecular_function	identical protein binding
L	0042867	biological_process	pyruvate catabolic process
L	0045121	cellular_component	membrane raft
L	0051287	molecular_function	NAD binding
L	0070062	cellular_component	extracellular exosome
L	1990204	cellular_component	oxidoreductase complex

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU190-SER196

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	HIS193
J	HIS193
K	HIS193
L	HIS193
B	HIS193
C	HIS193
D	HIS193
E	HIS193
F	HIS193
G	HIS193
H	HIS193
I	HIS193

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	GLN30
J	GLN30
K	GLN30
L	GLN30
B	GLN30
C	GLN30
D	GLN30
E	GLN30
F	GLN30
G	GLN30
H	GLN30
I	GLN30

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:11276087

Chain	Residue	Details
A	ARG99
J	ARG99
K	ARG99
L	ARG99
B	ARG99
C	ARG99
D	ARG99
E	ARG99
F	ARG99
G	ARG99
H	ARG99
I	ARG99

site_id	SWS_FT_FI4
Number of Residues	48
Details	BINDING:

Chain	Residue	Details
A	ARG106
C	ASN138
C	ARG169
C	THR248
D	ARG106
D	ASN138
D	ARG169
D	THR248
E	ARG106
E	ASN138
E	ARG169
A	ASN138
E	THR248
F	ARG106
F	ASN138
F	ARG169
F	THR248
G	ARG106
G	ASN138
G	ARG169
G	THR248
H	ARG106
A	ARG169
H	ASN138
H	ARG169
H	THR248
I	ARG106
I	ASN138
I	ARG169
I	THR248
J	ARG106
J	ASN138
J	ARG169
A	THR248
J	THR248
K	ARG106
K	ASN138
K	ARG169
K	THR248
L	ARG106
L	ASN138
L	ARG169
L	THR248
B	ARG106
B	ASN138
B	ARG169
B	THR248
C	ARG106

site_id	SWS_FT_FI5
Number of Residues	12
Details	MOD_RES: N-acetylalanine => ECO:0000269\|Ref.4

Chain	Residue	Details
A	ALA1
J	ALA1
K	ALA1
L	ALA1
B	ALA1
C	ALA1
D	ALA1
E	ALA1
F	ALA1
G	ALA1
H	ALA1
I	ALA1

site_id	SWS_FT_FI6
Number of Residues	48
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS6
C	LYS57
C	LYS118
C	LYS328
D	LYS6
D	LYS57
D	LYS118
D	LYS328
E	LYS6
E	LYS57
E	LYS118
A	LYS57
E	LYS328
F	LYS6
F	LYS57
F	LYS118
F	LYS328
G	LYS6
G	LYS57
G	LYS118
G	LYS328
H	LYS6
A	LYS118
H	LYS57
H	LYS118
H	LYS328
I	LYS6
I	LYS57
I	LYS118
I	LYS328
J	LYS6
J	LYS57
J	LYS118
A	LYS328
J	LYS328
K	LYS6
K	LYS57
K	LYS118
K	LYS328
L	LYS6
L	LYS57
L	LYS118
L	LYS328
B	LYS6
B	LYS57
B	LYS118
B	LYS328
C	LYS6

site_id	SWS_FT_FI7
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19690332

Chain	Residue	Details
A	SER43
J	SER43
K	SER43
L	SER43
B	SER43
C	SER43
D	SER43
E	SER43
F	SER43
G	SER43
H	SER43
I	SER43

site_id	SWS_FT_FI8
Number of Residues	12
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
A	TYR239
J	TYR239
K	TYR239
L	TYR239
B	TYR239
C	TYR239
D	TYR239
E	TYR239
F	TYR239
G	TYR239
H	TYR239
I	TYR239

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)