Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8RX0

(NEDD8)-CRL2VHL-MZ1-Brd4BD2-Ub(G76S, K48C)-UBE2R1(C93K, S138C, C191S, C223S)-Ub

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0006368	biological_process	transcription elongation by RNA polymerase II
C	0030891	cellular_component	VCB complex
C	0070449	cellular_component	elongin complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005730	cellular_component	nucleolus
E	0005829	cellular_component	cytosol
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0030163	biological_process	protein catabolic process
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0030891	cellular_component	VCB complex
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0044877	molecular_function	protein-containing complex binding
E	0097193	biological_process	intrinsic apoptotic signaling pathway
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0160072	molecular_function	ubiquitin ligase complex scaffold activity
G	0000082	biological_process	G1/S transition of mitotic cell cycle
G	0000209	biological_process	protein polyubiquitination
G	0004842	molecular_function	ubiquitin-protein transferase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006270	biological_process	DNA replication initiation
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0016567	biological_process	protein ubiquitination
G	0016607	cellular_component	nuclear speck
G	0016740	molecular_function	transferase activity
G	0019787	molecular_function	ubiquitin-like protein transferase activity
G	0032446	biological_process	protein modification by small protein conjugation
G	0035458	biological_process	cellular response to interferon-beta
G	0036211	biological_process	protein modification process
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0043525	biological_process	positive regulation of neuron apoptotic process
G	0061631	molecular_function	ubiquitin conjugating enzyme activity
G	0070848	biological_process	response to growth factor
G	0070936	biological_process	protein K48-linked ubiquitination
G	0090261	biological_process	positive regulation of inclusion body assembly
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005829	cellular_component	cytosol
N	0006357	biological_process	regulation of transcription by RNA polymerase II
N	0006508	biological_process	proteolysis
N	0006511	biological_process	ubiquitin-dependent protein catabolic process
N	0008104	biological_process	protein localization
N	0009653	biological_process	anatomical structure morphogenesis
N	0016567	biological_process	protein ubiquitination
N	0019941	biological_process	modification-dependent protein catabolic process
N	0030162	biological_process	regulation of proteolysis
N	0031386	molecular_function	protein tag activity
N	0031625	molecular_function	ubiquitin protein ligase binding
N	0036211	biological_process	protein modification process
N	0045116	biological_process	protein neddylation
N	0070062	cellular_component	extracellular exosome
N	0098978	cellular_component	glutamatergic synapse
N	0150052	biological_process	regulation of postsynapse assembly
R	0000165	biological_process	MAPK cascade
R	0000209	biological_process	protein polyubiquitination
R	0004842	molecular_function	ubiquitin-protein transferase activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006281	biological_process	DNA repair
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0006513	biological_process	protein monoubiquitination
R	0006974	biological_process	DNA damage response
R	0007283	biological_process	spermatogenesis
R	0008270	molecular_function	zinc ion binding
R	0016567	biological_process	protein ubiquitination
R	0016740	molecular_function	transferase activity
R	0019005	cellular_component	SCF ubiquitin ligase complex
R	0019788	molecular_function	NEDD8 transferase activity
R	0030163	biological_process	protein catabolic process
R	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R	0031461	cellular_component	cullin-RING ubiquitin ligase complex
R	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
R	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
R	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
R	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
R	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
R	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
R	0031625	molecular_function	ubiquitin protein ligase binding
R	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
R	0032480	biological_process	negative regulation of type I interferon production
R	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
R	0034644	biological_process	cellular response to UV
R	0042110	biological_process	T cell activation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0043687	biological_process	post-translational protein modification
R	0045116	biological_process	protein neddylation
R	0045732	biological_process	positive regulation of protein catabolic process
R	0046872	molecular_function	metal ion binding
R	0060090	molecular_function	molecular adaptor activity
R	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
R	0061630	molecular_function	ubiquitin protein ligase activity
R	0061663	molecular_function	NEDD8 ligase activity
R	0062197	biological_process	cellular response to chemical stress
R	0070936	biological_process	protein K48-linked ubiquitination
R	0071230	biological_process	cellular response to amino acid stimulus
R	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
R	0097602	molecular_function	cullin family protein binding
R	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
R	1900076	biological_process	regulation of cellular response to insulin stimulus
R	1902499	biological_process	positive regulation of protein autoubiquitination
R	1902883	biological_process	negative regulation of response to oxidative stress
R	1904263	biological_process	positive regulation of TORC1 signaling

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. WHPNIyet.GdVCIsiL

Chain	Residue	Details
G	TRP82-LEU97

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
U	LYS27-ASP52
N	LYS27-ASP52

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
E	ILE718-ALA745

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	45
Details	ZN_FING: RING-type => ECO:0000255\|PROSITE-ProRule:PRU00175

Chain	Residue	Details
R	CYS53-ASN98
N	ILE44

site_id	SWS_FT_FI2
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269\|PubMed:11961546, ECO:0007744\|PDB:1LDJ, ECO:0007744\|PDB:1LDK, ECO:0007744\|PDB:1U6G, ECO:0007744\|PDB:2HYE, ECO:0007744\|PDB:3DPL, ECO:0007744\|PDB:3DQV, ECO:0007744\|PDB:3RTR, ECO:0007744\|PDB:4F52, ECO:0007744\|PDB:4P5O

Chain	Residue	Details
R	CYS42
R	CYS45
R	CYS75
R	HIS77
R	HIS80
R	CYS94
R	ASP97

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:11961546, ECO:0007744\|PDB:1LDJ, ECO:0007744\|PDB:1LDK, ECO:0007744\|PDB:1U6G, ECO:0007744\|PDB:2HYE, ECO:0007744\|PDB:3DPL, ECO:0007744\|PDB:3DQV, ECO:0007744\|PDB:3RTR, ECO:0007744\|PDB:4F52, ECO:0007744\|PDB:4P5O, ECO:0007744\|PDB:7Z8B

Chain	Residue	Details
R	CYS53
R	CYS56
R	CYS68
R	HIS82

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:11961546, ECO:0007744\|PDB:1LDJ, ECO:0007744\|PDB:1LDK, ECO:0007744\|PDB:1U6G, ECO:0007744\|PDB:2HYE, ECO:0007744\|PDB:3DQV, ECO:0007744\|PDB:3RTR, ECO:0007744\|PDB:4F52, ECO:0007744\|PDB:4P5O

Chain	Residue	Details
R	CYS83

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by CK2 => ECO:0000269\|PubMed:11546811

Chain	Residue	Details
G	SER236

site_id	SWS_FT_FI6
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
U	LYS6

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
U	GLY76

site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144

Chain	Residue	Details
U	LYS11
U	LYS48

site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860

Chain	Residue	Details
U	LYS27

site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127

Chain	Residue	Details
U	LYS29

site_id	SWS_FT_FI11
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577

Chain	Residue	Details
U	LYS33

site_id	SWS_FT_FI12
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106

Chain	Residue	Details
U	LYS63

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)