8RB2

The crystal structure of DNA-bound human MutSbeta (MSH2_E749A/MSH3_E976A) in the canonical mismatch bound conformation with ADP bound in MSH2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0000400	molecular_function	four-way junction DNA binding
A	0000781	cellular_component	chromosome, telomeric region
A	0002204	biological_process	somatic recombination of immunoglobulin genes involved in immune response
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003684	molecular_function	damaged DNA binding
A	0003690	molecular_function	double-stranded DNA binding
A	0003697	molecular_function	single-stranded DNA binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0006281	biological_process	DNA repair
A	0006298	biological_process	mismatch repair
A	0006301	biological_process	DNA damage tolerance
A	0006312	biological_process	mitotic recombination
A	0006974	biological_process	DNA damage response
A	0008047	molecular_function	enzyme activator activity
A	0008094	molecular_function	ATP-dependent activity, acting on DNA
A	0008584	biological_process	male gonad development
A	0010165	biological_process	response to X-ray
A	0010224	biological_process	response to UV-B
A	0016020	cellular_component	membrane
A	0016447	biological_process	somatic recombination of immunoglobulin gene segments
A	0016887	molecular_function	ATP hydrolysis activity
A	0019237	molecular_function	centromeric DNA binding
A	0019724	biological_process	B cell mediated immunity
A	0030183	biological_process	B cell differentiation
A	0030983	molecular_function	mismatched DNA binding
A	0032137	molecular_function	guanine/thymine mispair binding
A	0032139	molecular_function	dinucleotide insertion or deletion binding
A	0032142	molecular_function	single guanine insertion binding
A	0032143	molecular_function	single thymine insertion binding
A	0032181	molecular_function	dinucleotide repeat insertion binding
A	0032301	cellular_component	MutSalpha complex
A	0032302	cellular_component	MutSbeta complex
A	0032357	molecular_function	oxidized purine DNA binding
A	0032405	molecular_function	MutLalpha complex binding
A	0042803	molecular_function	protein homodimerization activity
A	0043524	biological_process	negative regulation of neuron apoptotic process
A	0043531	molecular_function	ADP binding
A	0043570	biological_process	maintenance of DNA repeat elements
A	0045190	biological_process	isotype switching
A	0045910	biological_process	negative regulation of DNA recombination
A	0048298	biological_process	positive regulation of isotype switching to IgA isotypes
A	0048304	biological_process	positive regulation of isotype switching to IgG isotypes
A	0140664	molecular_function	ATP-dependent DNA damage sensor activity
B	0000166	molecular_function	nucleotide binding
B	0003677	molecular_function	DNA binding
B	0003690	molecular_function	double-stranded DNA binding
B	0003697	molecular_function	single-stranded DNA binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0006281	biological_process	DNA repair
B	0006298	biological_process	mismatch repair
B	0006312	biological_process	mitotic recombination
B	0006974	biological_process	DNA damage response
B	0016020	cellular_component	membrane
B	0016447	biological_process	somatic recombination of immunoglobulin gene segments
B	0019899	molecular_function	enzyme binding
B	0030983	molecular_function	mismatched DNA binding
B	0032137	molecular_function	guanine/thymine mispair binding
B	0032139	molecular_function	dinucleotide insertion or deletion binding
B	0032142	molecular_function	single guanine insertion binding
B	0032181	molecular_function	dinucleotide repeat insertion binding
B	0032302	cellular_component	MutSbeta complex
B	0032357	molecular_function	oxidized purine DNA binding
B	0042803	molecular_function	protein homodimerization activity
B	0043570	biological_process	maintenance of DNA repeat elements
B	0045910	biological_process	negative regulation of DNA recombination
B	0140664	molecular_function	ATP-dependent DNA damage sensor activity
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0000400	molecular_function	four-way junction DNA binding
E	0000781	cellular_component	chromosome, telomeric region
E	0002204	biological_process	somatic recombination of immunoglobulin genes involved in immune response
E	0003677	molecular_function	DNA binding
E	0003682	molecular_function	chromatin binding
E	0003684	molecular_function	damaged DNA binding
E	0003690	molecular_function	double-stranded DNA binding
E	0003697	molecular_function	single-stranded DNA binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0006281	biological_process	DNA repair
E	0006298	biological_process	mismatch repair
E	0006301	biological_process	DNA damage tolerance
E	0006312	biological_process	mitotic recombination
E	0006974	biological_process	DNA damage response
E	0008047	molecular_function	enzyme activator activity
E	0008094	molecular_function	ATP-dependent activity, acting on DNA
E	0008584	biological_process	male gonad development
E	0010165	biological_process	response to X-ray
E	0010224	biological_process	response to UV-B
E	0016020	cellular_component	membrane
E	0016447	biological_process	somatic recombination of immunoglobulin gene segments
E	0016887	molecular_function	ATP hydrolysis activity
E	0019237	molecular_function	centromeric DNA binding
E	0019724	biological_process	B cell mediated immunity
E	0030183	biological_process	B cell differentiation
E	0030983	molecular_function	mismatched DNA binding
E	0032137	molecular_function	guanine/thymine mispair binding
E	0032139	molecular_function	dinucleotide insertion or deletion binding
E	0032142	molecular_function	single guanine insertion binding
E	0032143	molecular_function	single thymine insertion binding
E	0032181	molecular_function	dinucleotide repeat insertion binding
E	0032301	cellular_component	MutSalpha complex
E	0032302	cellular_component	MutSbeta complex
E	0032357	molecular_function	oxidized purine DNA binding
E	0032405	molecular_function	MutLalpha complex binding
E	0042803	molecular_function	protein homodimerization activity
E	0043524	biological_process	negative regulation of neuron apoptotic process
E	0043531	molecular_function	ADP binding
E	0043570	biological_process	maintenance of DNA repeat elements
E	0045190	biological_process	isotype switching
E	0045910	biological_process	negative regulation of DNA recombination
E	0048298	biological_process	positive regulation of isotype switching to IgA isotypes
E	0048304	biological_process	positive regulation of isotype switching to IgG isotypes
E	0140664	molecular_function	ATP-dependent DNA damage sensor activity
F	0000166	molecular_function	nucleotide binding
F	0003677	molecular_function	DNA binding
F	0003690	molecular_function	double-stranded DNA binding
F	0003697	molecular_function	single-stranded DNA binding
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0006281	biological_process	DNA repair
F	0006298	biological_process	mismatch repair
F	0006312	biological_process	mitotic recombination
F	0006974	biological_process	DNA damage response
F	0016020	cellular_component	membrane
F	0016447	biological_process	somatic recombination of immunoglobulin gene segments
F	0019899	molecular_function	enzyme binding
F	0030983	molecular_function	mismatched DNA binding
F	0032137	molecular_function	guanine/thymine mispair binding
F	0032139	molecular_function	dinucleotide insertion or deletion binding
F	0032142	molecular_function	single guanine insertion binding
F	0032181	molecular_function	dinucleotide repeat insertion binding
F	0032302	cellular_component	MutSbeta complex
F	0032357	molecular_function	oxidized purine DNA binding
F	0042803	molecular_function	protein homodimerization activity
F	0043570	biological_process	maintenance of DNA repeat elements
F	0045910	biological_process	negative regulation of DNA recombination
F	0140664	molecular_function	ATP-dependent DNA damage sensor activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	140
Details	Region: {"description":"Interaction with EXO1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	28
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P43247","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"24882211","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	10
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"24882211","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---