Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8QP8

Cryo-EM Structure of Pre-B Complex (core part)

Functional Information from GO Data

Chain	GOid	namespace	contents
7	0000389	biological_process	mRNA 3'-splice site recognition
7	0000398	biological_process	mRNA splicing, via spliceosome
7	0003723	molecular_function	RNA binding
7	0005515	molecular_function	protein binding
7	0005634	cellular_component	nucleus
7	0005654	cellular_component	nucleoplasm
7	0005681	cellular_component	spliceosomal complex
7	0005684	cellular_component	U2-type spliceosomal complex
7	0005686	cellular_component	U2 snRNP
7	0006397	biological_process	mRNA processing
7	0008380	biological_process	RNA splicing
7	0016607	cellular_component	nuclear speck
7	0071004	cellular_component	U2-type prespliceosome
7	0071005	cellular_component	U2-type precatalytic spliceosome
7	0071013	cellular_component	catalytic step 2 spliceosome
7	1903241	biological_process	U2-type prespliceosome assembly
A	0000244	biological_process	spliceosomal tri-snRNP complex assembly
A	0000375	biological_process	RNA splicing, via transesterification reactions
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005681	cellular_component	spliceosomal complex
A	0005682	cellular_component	U5 snRNP
A	0006397	biological_process	mRNA processing
A	0008233	molecular_function	peptidase activity
A	0008380	biological_process	RNA splicing
A	0016020	cellular_component	membrane
A	0016607	cellular_component	nuclear speck
A	0017070	molecular_function	U6 snRNA binding
A	0030532	cellular_component	small nuclear ribonucleoprotein complex
A	0030619	molecular_function	U1 snRNA binding
A	0030620	molecular_function	U2 snRNA binding
A	0030623	molecular_function	U5 snRNA binding
A	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
A	0070530	molecular_function	K63-linked polyubiquitin modification-dependent protein binding
A	0071005	cellular_component	U2-type precatalytic spliceosome
A	0071006	cellular_component	U2-type catalytic step 1 spliceosome
A	0071007	cellular_component	U2-type catalytic step 2 spliceosome
A	0071013	cellular_component	catalytic step 2 spliceosome
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0097157	molecular_function	pre-mRNA intronic binding
A	0140492	molecular_function	metal-dependent deubiquitinase activity
C	0000398	biological_process	mRNA splicing, via spliceosome
C	0003723	molecular_function	RNA binding
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005681	cellular_component	spliceosomal complex
C	0005829	cellular_component	cytosol
C	0006397	biological_process	mRNA processing
C	0008380	biological_process	RNA splicing
C	0015030	cellular_component	Cajal body
C	0016020	cellular_component	membrane
C	0016607	cellular_component	nuclear speck
C	0030623	molecular_function	U5 snRNA binding
C	0042220	biological_process	response to cocaine
C	0043231	cellular_component	intracellular membrane-bounded organelle
C	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
C	0071005	cellular_component	U2-type precatalytic spliceosome
C	0071007	cellular_component	U2-type catalytic step 2 spliceosome
C	0071013	cellular_component	catalytic step 2 spliceosome
C	0071466	biological_process	cellular response to xenobiotic stimulus
D	0000245	biological_process	spliceosomal complex assembly
D	0000375	biological_process	RNA splicing, via transesterification reactions
D	0000398	biological_process	mRNA splicing, via spliceosome
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005681	cellular_component	spliceosomal complex
D	0005682	cellular_component	U5 snRNP
D	0005829	cellular_component	cytosol
D	0006397	biological_process	mRNA processing
D	0008380	biological_process	RNA splicing
D	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
D	0051301	biological_process	cell division
D	0071005	cellular_component	U2-type precatalytic spliceosome
G	0000354	biological_process	cis assembly of pre-catalytic spliceosome
G	0000375	biological_process	RNA splicing, via transesterification reactions
G	0000398	biological_process	mRNA splicing, via spliceosome
G	0000785	cellular_component	chromatin
G	0003723	molecular_function	RNA binding
G	0003724	molecular_function	RNA helicase activity
G	0003729	molecular_function	mRNA binding
G	0004386	molecular_function	helicase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005681	cellular_component	spliceosomal complex
G	0005682	cellular_component	U5 snRNP
G	0005694	cellular_component	chromosome
G	0005730	cellular_component	nucleolus
G	0006397	biological_process	mRNA processing
G	0008380	biological_process	RNA splicing
G	0016787	molecular_function	hydrolase activity
G	0016887	molecular_function	ATP hydrolysis activity
G	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
G	0062176	biological_process	R-loop processing
G	0070062	cellular_component	extracellular exosome
G	0071013	cellular_component	catalytic step 2 spliceosome
J	0000244	biological_process	spliceosomal tri-snRNP complex assembly
J	0000375	biological_process	RNA splicing, via transesterification reactions
J	0000398	biological_process	mRNA splicing, via spliceosome
J	0003723	molecular_function	RNA binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005681	cellular_component	spliceosomal complex
J	0005829	cellular_component	cytosol
J	0006397	biological_process	mRNA processing
J	0008380	biological_process	RNA splicing
J	0015030	cellular_component	Cajal body
J	0016607	cellular_component	nuclear speck
J	0032991	cellular_component	protein-containing complex
J	0042802	molecular_function	identical protein binding
J	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
J	0071005	cellular_component	U2-type precatalytic spliceosome
L	0000244	biological_process	spliceosomal tri-snRNP complex assembly
L	0000398	biological_process	mRNA splicing, via spliceosome
L	0003723	molecular_function	RNA binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005681	cellular_component	spliceosomal complex
L	0005684	cellular_component	U2-type spliceosomal complex
L	0005687	cellular_component	U4 snRNP
L	0005690	cellular_component	U4atac snRNP
L	0006397	biological_process	mRNA processing
L	0008380	biological_process	RNA splicing
L	0015030	cellular_component	Cajal body
L	0016607	cellular_component	nuclear speck
L	0030621	molecular_function	U4 snRNA binding
L	0030622	molecular_function	U4atac snRNA binding
L	0030674	molecular_function	protein-macromolecule adaptor activity
L	0042802	molecular_function	identical protein binding
L	0043021	molecular_function	ribonucleoprotein complex binding
L	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
L	0048254	biological_process	snoRNA localization
L	0070990	molecular_function	snRNP binding
L	0071005	cellular_component	U2-type precatalytic spliceosome
L	0071011	cellular_component	precatalytic spliceosome
L	0071166	biological_process	ribonucleoprotein complex localization
L	0071339	cellular_component	MLL1 complex
L	0097526	cellular_component	spliceosomal tri-snRNP complex
N	0000244	biological_process	spliceosomal tri-snRNP complex assembly
N	0000245	biological_process	spliceosomal complex assembly
N	0000375	biological_process	RNA splicing, via transesterification reactions
N	0000398	biological_process	mRNA splicing, via spliceosome
N	0003723	molecular_function	RNA binding
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005681	cellular_component	spliceosomal complex
N	0005682	cellular_component	U5 snRNP
N	0006397	biological_process	mRNA processing
N	0006403	biological_process	RNA localization
N	0008380	biological_process	RNA splicing
N	0016020	cellular_component	membrane
N	0016607	cellular_component	nuclear speck
N	0030674	molecular_function	protein-macromolecule adaptor activity
N	0042802	molecular_function	identical protein binding
N	0043021	molecular_function	ribonucleoprotein complex binding
N	0045944	biological_process	positive regulation of transcription by RNA polymerase II
N	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
N	0071005	cellular_component	U2-type precatalytic spliceosome
N	0071013	cellular_component	catalytic step 2 spliceosome
R	0000398	biological_process	mRNA splicing, via spliceosome
R	0003723	molecular_function	RNA binding
R	0003729	molecular_function	mRNA binding
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
R	0048025	biological_process	negative regulation of mRNA splicing, via spliceosome
S	0000387	biological_process	spliceosomal snRNP assembly
S	0000398	biological_process	mRNA splicing, via spliceosome
S	0000481	biological_process	maturation of 5S rRNA
S	0003723	molecular_function	RNA binding
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005681	cellular_component	spliceosomal complex
S	0005737	cellular_component	cytoplasm
S	0005794	cellular_component	Golgi apparatus
S	0005829	cellular_component	cytosol
S	0006397	biological_process	mRNA processing
S	0008380	biological_process	RNA splicing
S	0015030	cellular_component	Cajal body
S	0016607	cellular_component	nuclear speck
S	0045292	biological_process	mRNA cis splicing, via spliceosome
S	0045585	biological_process	positive regulation of cytotoxic T cell differentiation
S	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
S	0071005	cellular_component	U2-type precatalytic spliceosome
S	0071013	cellular_component	catalytic step 2 spliceosome
U	0000245	biological_process	spliceosomal complex assembly
U	0000398	biological_process	mRNA splicing, via spliceosome
U	0004843	molecular_function	cysteine-type deubiquitinase activity
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005681	cellular_component	spliceosomal complex
U	0006397	biological_process	mRNA processing
U	0008380	biological_process	RNA splicing
U	0016787	molecular_function	hydrolase activity
U	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
U	0046872	molecular_function	metal ion binding
U	0051301	biological_process	cell division
X	0000398	biological_process	mRNA splicing, via spliceosome
X	0003676	molecular_function	nucleic acid binding
X	0005515	molecular_function	protein binding
X	0005575	cellular_component	cellular_component
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0006397	biological_process	mRNA processing
X	0008150	biological_process	biological_process
X	0008380	biological_process	RNA splicing
X	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADRmI

Chain	Residue	Details
G	VAL547-ILE555

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269\|Ref.4, ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:22223895

Chain	Residue	Details
A	ALA2
C	ASP204
C	ASN258

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
A	SER859
U	CYS139
U	HIS155
U	HIS161

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER1358
R	ARG158
R	ARG168
R	ARG181

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0007744\|PubMed:24129315

Chain	Residue	Details
A	LYS1425
G	SER109
J	LYS244
J	LYS252

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS1463
G	LYS811

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
S	SER474
S	SER621

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648

Chain	Residue	Details
S	SER486

site_id	SWS_FT_FI8
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
S	SER521
S	SER596
S	SER598

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
S	SER591

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17525332

Chain	Residue	Details
S	THR695
7	LYS131
7	LYS424
7	LYS499

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:19690332

Chain	Residue	Details
S	THR764
7	LYS686
L	LYS478

site_id	SWS_FT_FI12
Number of Residues	20
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
S	LYS125
S	LYS648
S	LYS657
S	LYS684
S	LYS699
S	LYS709
S	LYS723
S	LYS749
S	LYS758
S	LYS775
S	LYS780
S	LYS791
S	LYS133
S	LYS277
S	LYS329
S	LYS336
S	LYS400
S	LYS414
S	LYS548

site_id	SWS_FT_FI13
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:25114211, ECO:0007744\|PubMed:25218447, ECO:0007744\|PubMed:25755297, ECO:0007744\|PubMed:25772364, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
S	LYS141

site_id	SWS_FT_FI14
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:25218447, ECO:0007744\|PubMed:25755297, ECO:0007744\|PubMed:25772364, ECO:0007744\|PubMed:28112733

Chain	Residue	Details
S	LYS147
S	LYS188

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)