8QJ7

Cryo-EM structure of human DNA polymerase alpha-primase in pre-initiation stage 1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000731	biological_process	DNA synthesis involved in DNA repair
A	0000785	cellular_component	chromatin
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003688	molecular_function	DNA replication origin binding
A	0003697	molecular_function	single-stranded DNA binding
A	0003887	molecular_function	DNA-directed DNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005635	cellular_component	nuclear envelope
A	0005654	cellular_component	nucleoplasm
A	0005658	cellular_component	alpha DNA polymerase:primase complex
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006260	biological_process	DNA replication
A	0006261	biological_process	DNA-templated DNA replication
A	0006269	biological_process	DNA replication, synthesis of primer
A	0006270	biological_process	DNA replication initiation
A	0006271	biological_process	DNA strand elongation involved in DNA replication
A	0006272	biological_process	leading strand elongation
A	0006273	biological_process	lagging strand elongation
A	0006281	biological_process	DNA repair
A	0006289	biological_process	nucleotide-excision repair
A	0006303	biological_process	double-strand break repair via nonhomologous end joining
A	0008270	molecular_function	zinc ion binding
A	0016363	cellular_component	nuclear matrix
A	0019901	molecular_function	protein kinase binding
A	0031981	cellular_component	nuclear lumen
A	0032479	biological_process	regulation of type I interferon production
A	0046872	molecular_function	metal ion binding
A	1902975	biological_process	mitotic DNA replication initiation
A	1904161	biological_process	DNA synthesis involved in UV-damage excision repair
B	0003674	molecular_function	molecular_function
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005658	cellular_component	alpha DNA polymerase:primase complex
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006269	biological_process	DNA replication, synthesis of primer
B	0006270	biological_process	DNA replication initiation
B	0006606	biological_process	protein import into nucleus
C	0000287	molecular_function	magnesium ion binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003896	molecular_function	DNA primase activity
C	0005515	molecular_function	protein binding
C	0005654	cellular_component	nucleoplasm
C	0005658	cellular_component	alpha DNA polymerase:primase complex
C	0006260	biological_process	DNA replication
C	0006269	biological_process	DNA replication, synthesis of primer
C	0006270	biological_process	DNA replication initiation
C	0008270	molecular_function	zinc ion binding
C	0016020	cellular_component	membrane
C	0016779	molecular_function	nucleotidyltransferase activity
C	0032553	molecular_function	ribonucleotide binding
C	0046872	molecular_function	metal ion binding
C	1990077	cellular_component	primosome complex
D	0003677	molecular_function	DNA binding
D	0005515	molecular_function	protein binding
D	0005654	cellular_component	nucleoplasm
D	0005658	cellular_component	alpha DNA polymerase:primase complex
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006269	biological_process	DNA replication, synthesis of primer
D	0006270	biological_process	DNA replication initiation
D	0046872	molecular_function	metal ion binding
D	0051539	molecular_function	4 iron, 4 sulfur cluster binding
D	0071667	molecular_function	DNA/RNA hybrid binding
D	1903934	biological_process	positive regulation of DNA primase activity
D	1990077	cellular_component	primosome complex

Functional Information from PROSITE/UniProt

site_id	PS00116
Number of Residues	9
Details	DNA_POLYMERASE_B DNA polymerase family B signature. YGDTDSIMI

Chain	Residue	Details
A	TYR1000-ILE1008

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:17893144, ECO:0000269|PubMed:25550159, ECO:0000269|PubMed:26975377

Chain	Residue	Details
D	CYS287
D	CYS367
D	CYS424

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site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:17893144, ECO:0000269|PubMed:25550159

Chain	Residue	Details
D	CYS384
B	THR130
A	CYS1310
A	CYS1315
A	CYS1348
A	CYS1353
A	CYS1371
A	CYS1374

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
D	THR470
C	CYS122
C	CYS128
C	CYS131

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER147

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER152

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site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER154

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site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER209

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site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P33609

Chain	Residue	Details
A	LYS224

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site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	THR406

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site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P33609

Chain	Residue	Details
A	LYS970

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