8Q6T

Helical reconstruction of the relaxed thick filament from FIB milled left ventricular mouse myofibrils

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000146	molecular_function	microfilament motor activity
A	0001725	cellular_component	stress fiber
A	0002026	biological_process	regulation of the force of heart contraction
A	0002027	biological_process	regulation of heart rate
A	0003009	biological_process	skeletal muscle contraction
A	0003774	molecular_function	cytoskeletal motor activity
A	0003779	molecular_function	actin binding
A	0005516	molecular_function	calmodulin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005859	cellular_component	muscle myosin complex
A	0006936	biological_process	muscle contraction
A	0006941	biological_process	striated muscle contraction
A	0007512	biological_process	adult heart development
A	0014728	biological_process	regulation of the force of skeletal muscle contraction
A	0014883	biological_process	transition between fast and slow fiber
A	0014898	biological_process	cardiac muscle hypertrophy in response to stress
A	0016459	cellular_component	myosin complex
A	0016460	cellular_component	myosin II complex
A	0016887	molecular_function	ATP hydrolysis activity
A	0030016	cellular_component	myofibril
A	0030017	cellular_component	sarcomere
A	0030018	cellular_component	Z disc
A	0030049	biological_process	muscle filament sliding
A	0031449	biological_process	regulation of slow-twitch skeletal muscle fiber contraction
A	0032982	cellular_component	myosin filament
A	0042802	molecular_function	identical protein binding
A	0044877	molecular_function	protein-containing complex binding
A	0045214	biological_process	sarcomere organization
A	0046034	biological_process	ATP metabolic process
A	0051015	molecular_function	actin filament binding
A	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
A	0060048	biological_process	cardiac muscle contraction
B	0000146	molecular_function	microfilament motor activity
B	0001725	cellular_component	stress fiber
B	0002026	biological_process	regulation of the force of heart contraction
B	0002027	biological_process	regulation of heart rate
B	0003009	biological_process	skeletal muscle contraction
B	0003774	molecular_function	cytoskeletal motor activity
B	0003779	molecular_function	actin binding
B	0005516	molecular_function	calmodulin binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005859	cellular_component	muscle myosin complex
B	0006936	biological_process	muscle contraction
B	0006941	biological_process	striated muscle contraction
B	0007512	biological_process	adult heart development
B	0014728	biological_process	regulation of the force of skeletal muscle contraction
B	0014883	biological_process	transition between fast and slow fiber
B	0014898	biological_process	cardiac muscle hypertrophy in response to stress
B	0016459	cellular_component	myosin complex
B	0016460	cellular_component	myosin II complex
B	0016887	molecular_function	ATP hydrolysis activity
B	0030016	cellular_component	myofibril
B	0030017	cellular_component	sarcomere
B	0030018	cellular_component	Z disc
B	0030049	biological_process	muscle filament sliding
B	0031449	biological_process	regulation of slow-twitch skeletal muscle fiber contraction
B	0032982	cellular_component	myosin filament
B	0042802	molecular_function	identical protein binding
B	0044877	molecular_function	protein-containing complex binding
B	0045214	biological_process	sarcomere organization
B	0046034	biological_process	ATP metabolic process
B	0051015	molecular_function	actin filament binding
B	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
B	0060048	biological_process	cardiac muscle contraction
C	0005509	molecular_function	calcium ion binding
D	0005509	molecular_function	calcium ion binding
E	0002026	biological_process	regulation of the force of heart contraction
E	0003007	biological_process	heart morphogenesis
E	0005509	molecular_function	calcium ion binding
E	0005737	cellular_component	cytoplasm
E	0007507	biological_process	heart development
E	0008307	molecular_function	structural constituent of muscle
E	0009791	biological_process	post-embryonic development
E	0016459	cellular_component	myosin complex
E	0030016	cellular_component	myofibril
E	0031672	cellular_component	A band
E	0042694	biological_process	muscle cell fate specification
E	0043292	cellular_component	contractile muscle fiber
E	0046872	molecular_function	metal ion binding
E	0055001	biological_process	muscle cell development
E	0055003	biological_process	cardiac myofibril assembly
E	0060047	biological_process	heart contraction
E	0060048	biological_process	cardiac muscle contraction
E	0098735	biological_process	positive regulation of the force of heart contraction
F	0002026	biological_process	regulation of the force of heart contraction
F	0003007	biological_process	heart morphogenesis
F	0005509	molecular_function	calcium ion binding
F	0005737	cellular_component	cytoplasm
F	0007507	biological_process	heart development
F	0008307	molecular_function	structural constituent of muscle
F	0009791	biological_process	post-embryonic development
F	0016459	cellular_component	myosin complex
F	0030016	cellular_component	myofibril
F	0031672	cellular_component	A band
F	0042694	biological_process	muscle cell fate specification
F	0043292	cellular_component	contractile muscle fiber
F	0046872	molecular_function	metal ion binding
F	0055001	biological_process	muscle cell development
F	0055003	biological_process	cardiac myofibril assembly
F	0060047	biological_process	heart contraction
F	0060048	biological_process	cardiac muscle contraction
F	0098735	biological_process	positive regulation of the force of heart contraction
H	0000146	molecular_function	microfilament motor activity
H	0001725	cellular_component	stress fiber
H	0002026	biological_process	regulation of the force of heart contraction
H	0002027	biological_process	regulation of heart rate
H	0003009	biological_process	skeletal muscle contraction
H	0003774	molecular_function	cytoskeletal motor activity
H	0003779	molecular_function	actin binding
H	0005516	molecular_function	calmodulin binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005859	cellular_component	muscle myosin complex
H	0006936	biological_process	muscle contraction
H	0006941	biological_process	striated muscle contraction
H	0007512	biological_process	adult heart development
H	0014728	biological_process	regulation of the force of skeletal muscle contraction
H	0014883	biological_process	transition between fast and slow fiber
H	0014898	biological_process	cardiac muscle hypertrophy in response to stress
H	0016459	cellular_component	myosin complex
H	0016460	cellular_component	myosin II complex
H	0016887	molecular_function	ATP hydrolysis activity
H	0030016	cellular_component	myofibril
H	0030017	cellular_component	sarcomere
H	0030018	cellular_component	Z disc
H	0030049	biological_process	muscle filament sliding
H	0031449	biological_process	regulation of slow-twitch skeletal muscle fiber contraction
H	0032982	cellular_component	myosin filament
H	0042802	molecular_function	identical protein binding
H	0044877	molecular_function	protein-containing complex binding
H	0045214	biological_process	sarcomere organization
H	0046034	biological_process	ATP metabolic process
H	0051015	molecular_function	actin filament binding
H	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
H	0060048	biological_process	cardiac muscle contraction
J	0005509	molecular_function	calcium ion binding
K	0005509	molecular_function	calcium ion binding
L	0002026	biological_process	regulation of the force of heart contraction
L	0003007	biological_process	heart morphogenesis
L	0005509	molecular_function	calcium ion binding
L	0005737	cellular_component	cytoplasm
L	0007507	biological_process	heart development
L	0008307	molecular_function	structural constituent of muscle
L	0009791	biological_process	post-embryonic development
L	0016459	cellular_component	myosin complex
L	0030016	cellular_component	myofibril
L	0031672	cellular_component	A band
L	0042694	biological_process	muscle cell fate specification
L	0043292	cellular_component	contractile muscle fiber
L	0046872	molecular_function	metal ion binding
L	0055001	biological_process	muscle cell development
L	0055003	biological_process	cardiac myofibril assembly
L	0060047	biological_process	heart contraction
L	0060048	biological_process	cardiac muscle contraction
L	0098735	biological_process	positive regulation of the force of heart contraction
M	0002026	biological_process	regulation of the force of heart contraction
M	0003007	biological_process	heart morphogenesis
M	0005509	molecular_function	calcium ion binding
M	0005737	cellular_component	cytoplasm
M	0007507	biological_process	heart development
M	0008307	molecular_function	structural constituent of muscle
M	0009791	biological_process	post-embryonic development
M	0016459	cellular_component	myosin complex
M	0030016	cellular_component	myofibril
M	0031672	cellular_component	A band
M	0042694	biological_process	muscle cell fate specification
M	0043292	cellular_component	contractile muscle fiber
M	0046872	molecular_function	metal ion binding
M	0055001	biological_process	muscle cell development
M	0055003	biological_process	cardiac myofibril assembly
M	0060047	biological_process	heart contraction
M	0060048	biological_process	cardiac muscle contraction
M	0098735	biological_process	positive regulation of the force of heart contraction
N	0000146	molecular_function	microfilament motor activity
N	0001725	cellular_component	stress fiber
N	0002026	biological_process	regulation of the force of heart contraction
N	0002027	biological_process	regulation of heart rate
N	0003009	biological_process	skeletal muscle contraction
N	0003774	molecular_function	cytoskeletal motor activity
N	0003779	molecular_function	actin binding
N	0005516	molecular_function	calmodulin binding
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0005859	cellular_component	muscle myosin complex
N	0006936	biological_process	muscle contraction
N	0006941	biological_process	striated muscle contraction
N	0007512	biological_process	adult heart development
N	0014728	biological_process	regulation of the force of skeletal muscle contraction
N	0014883	biological_process	transition between fast and slow fiber
N	0014898	biological_process	cardiac muscle hypertrophy in response to stress
N	0016459	cellular_component	myosin complex
N	0016460	cellular_component	myosin II complex
N	0016887	molecular_function	ATP hydrolysis activity
N	0030016	cellular_component	myofibril
N	0030017	cellular_component	sarcomere
N	0030018	cellular_component	Z disc
N	0030049	biological_process	muscle filament sliding
N	0031449	biological_process	regulation of slow-twitch skeletal muscle fiber contraction
N	0032982	cellular_component	myosin filament
N	0042802	molecular_function	identical protein binding
N	0044877	molecular_function	protein-containing complex binding
N	0045214	biological_process	sarcomere organization
N	0046034	biological_process	ATP metabolic process
N	0051015	molecular_function	actin filament binding
N	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
N	0060048	biological_process	cardiac muscle contraction
O	0000146	molecular_function	microfilament motor activity
O	0001725	cellular_component	stress fiber
O	0002026	biological_process	regulation of the force of heart contraction
O	0002027	biological_process	regulation of heart rate
O	0003009	biological_process	skeletal muscle contraction
O	0003774	molecular_function	cytoskeletal motor activity
O	0003779	molecular_function	actin binding
O	0005516	molecular_function	calmodulin binding
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0005859	cellular_component	muscle myosin complex
O	0006936	biological_process	muscle contraction
O	0006941	biological_process	striated muscle contraction
O	0007512	biological_process	adult heart development
O	0014728	biological_process	regulation of the force of skeletal muscle contraction
O	0014883	biological_process	transition between fast and slow fiber
O	0014898	biological_process	cardiac muscle hypertrophy in response to stress
O	0016459	cellular_component	myosin complex
O	0016460	cellular_component	myosin II complex
O	0016887	molecular_function	ATP hydrolysis activity
O	0030016	cellular_component	myofibril
O	0030017	cellular_component	sarcomere
O	0030018	cellular_component	Z disc
O	0030049	biological_process	muscle filament sliding
O	0031449	biological_process	regulation of slow-twitch skeletal muscle fiber contraction
O	0032982	cellular_component	myosin filament
O	0042802	molecular_function	identical protein binding
O	0044877	molecular_function	protein-containing complex binding
O	0045214	biological_process	sarcomere organization
O	0046034	biological_process	ATP metabolic process
O	0051015	molecular_function	actin filament binding
O	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
O	0060048	biological_process	cardiac muscle contraction
Q	0000146	molecular_function	microfilament motor activity
Q	0001725	cellular_component	stress fiber
Q	0002026	biological_process	regulation of the force of heart contraction
Q	0002027	biological_process	regulation of heart rate
Q	0003009	biological_process	skeletal muscle contraction
Q	0003774	molecular_function	cytoskeletal motor activity
Q	0003779	molecular_function	actin binding
Q	0005516	molecular_function	calmodulin binding
Q	0005524	molecular_function	ATP binding
Q	0005737	cellular_component	cytoplasm
Q	0005859	cellular_component	muscle myosin complex
Q	0006936	biological_process	muscle contraction
Q	0006941	biological_process	striated muscle contraction
Q	0007512	biological_process	adult heart development
Q	0014728	biological_process	regulation of the force of skeletal muscle contraction
Q	0014883	biological_process	transition between fast and slow fiber
Q	0014898	biological_process	cardiac muscle hypertrophy in response to stress
Q	0016459	cellular_component	myosin complex
Q	0016460	cellular_component	myosin II complex
Q	0016887	molecular_function	ATP hydrolysis activity
Q	0030016	cellular_component	myofibril
Q	0030017	cellular_component	sarcomere
Q	0030018	cellular_component	Z disc
Q	0030049	biological_process	muscle filament sliding
Q	0031449	biological_process	regulation of slow-twitch skeletal muscle fiber contraction
Q	0032982	cellular_component	myosin filament
Q	0042802	molecular_function	identical protein binding
Q	0044877	molecular_function	protein-containing complex binding
Q	0045214	biological_process	sarcomere organization
Q	0046034	biological_process	ATP metabolic process
Q	0051015	molecular_function	actin filament binding
Q	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
Q	0060048	biological_process	cardiac muscle contraction
R	0005509	molecular_function	calcium ion binding
S	0005509	molecular_function	calcium ion binding
T	0002026	biological_process	regulation of the force of heart contraction
T	0003007	biological_process	heart morphogenesis
T	0005509	molecular_function	calcium ion binding
T	0005737	cellular_component	cytoplasm
T	0007507	biological_process	heart development
T	0008307	molecular_function	structural constituent of muscle
T	0009791	biological_process	post-embryonic development
T	0016459	cellular_component	myosin complex
T	0030016	cellular_component	myofibril
T	0031672	cellular_component	A band
T	0042694	biological_process	muscle cell fate specification
T	0043292	cellular_component	contractile muscle fiber
T	0046872	molecular_function	metal ion binding
T	0055001	biological_process	muscle cell development
T	0055003	biological_process	cardiac myofibril assembly
T	0060047	biological_process	heart contraction
T	0060048	biological_process	cardiac muscle contraction
T	0098735	biological_process	positive regulation of the force of heart contraction
U	0002026	biological_process	regulation of the force of heart contraction
U	0003007	biological_process	heart morphogenesis
U	0005509	molecular_function	calcium ion binding
U	0005737	cellular_component	cytoplasm
U	0007507	biological_process	heart development
U	0008307	molecular_function	structural constituent of muscle
U	0009791	biological_process	post-embryonic development
U	0016459	cellular_component	myosin complex
U	0030016	cellular_component	myofibril
U	0031672	cellular_component	A band
U	0042694	biological_process	muscle cell fate specification
U	0043292	cellular_component	contractile muscle fiber
U	0046872	molecular_function	metal ion binding
U	0055001	biological_process	muscle cell development
U	0055003	biological_process	cardiac myofibril assembly
U	0060047	biological_process	heart contraction
U	0060048	biological_process	cardiac muscle contraction
U	0098735	biological_process	positive regulation of the force of heart contraction

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DQNRDGFIDknDL

Chain	Residue	Details
E	ASP37-LEU49

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448

Chain	Residue	Details
E	ASP37
L	ASN39
L	ASP41
L	ASP48
M	ASP37
M	ASN39
M	ASP41
M	ASP48
T	ASP37
T	ASN39
T	ASP41
E	ASN39
T	ASP48
U	ASP37
U	ASN39
U	ASP41
U	ASP48
E	ASP41
E	ASP48
F	ASP37
F	ASN39
F	ASP41
F	ASP48
L	ASP37

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	MOD_RES: Phosphoserine; by MLCK => ECO:0000269|PubMed:20445002, ECO:0000269|PubMed:22426213, ECO:0007744|PubMed:21183079

Chain	Residue	Details
E	SER14
T	SER15
U	SER14
U	SER15
E	SER15
F	SER14
F	SER15
L	SER14
L	SER15
M	SER14
M	SER15
T	SER14

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:22426213, ECO:0007744|PubMed:21183079

Chain	Residue	Details
E	SER19
H	THR1282
H	THR1309
H	THR1513
N	THR378
N	THR1282
N	THR1309
N	THR1513
O	THR378
O	THR1282
O	THR1309
F	SER19
O	THR1513
Q	THR378
Q	THR1282
Q	THR1309
Q	THR1513
L	SER19
M	SER19
T	SER19
U	SER19
B	THR1309
B	THR1513
H	THR378

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P08733

Chain	Residue	Details
E	THR52
F	THR52
L	THR52
M	THR52
T	THR52
U	THR52

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q02566

Chain	Residue	Details
A	SER1269
B	SER1269
H	SER1269
N	SER1269
O	SER1269
Q	SER1269

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P02563

Chain	Residue	Details
A	TYR1308
B	TYR1308
H	TYR1308
N	TYR1308
O	TYR1308
Q	TYR1308

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P02564

Chain	Residue	Details
A	SER1510
B	SER1510
H	SER1510
N	SER1510
O	SER1510
Q	SER1510

---