8Q25

Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
A	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
A	0045271	cellular_component	respiratory chain complex I
A	1902600	biological_process	proton transmembrane transport
B	0003954	molecular_function	NADH dehydrogenase activity
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
B	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
B	0009060	biological_process	aerobic respiration
B	0015990	biological_process	electron transport coupled proton transport
B	0016491	molecular_function	oxidoreductase activity
B	0032981	biological_process	mitochondrial respiratory chain complex I assembly
B	0045271	cellular_component	respiratory chain complex I
B	0046872	molecular_function	metal ion binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	0003954	molecular_function	NADH dehydrogenase activity
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
C	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
C	0016491	molecular_function	oxidoreductase activity
C	0031966	cellular_component	mitochondrial membrane
C	0032981	biological_process	mitochondrial respiratory chain complex I assembly
C	0045271	cellular_component	respiratory chain complex I
C	0072593	biological_process	reactive oxygen species metabolic process
C	1902600	biological_process	proton transmembrane transport
D	0003954	molecular_function	NADH dehydrogenase activity
D	0005654	cellular_component	nucleoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
D	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
D	0016491	molecular_function	oxidoreductase activity
D	0019826	molecular_function	oxygen sensor activity
D	0022008	biological_process	neurogenesis
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032981	biological_process	mitochondrial respiratory chain complex I assembly
D	0042063	biological_process	gliogenesis
D	0042775	biological_process	mitochondrial ATP synthesis coupled electron transport
D	0045271	cellular_component	respiratory chain complex I
D	0046872	molecular_function	metal ion binding
D	0051539	molecular_function	4 iron, 4 sulfur cluster binding
D	0061351	biological_process	neural precursor cell proliferation
D	0071453	biological_process	cellular response to oxygen levels
D	1902600	biological_process	proton transmembrane transport
E	0003954	molecular_function	NADH dehydrogenase activity
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
E	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
E	0016491	molecular_function	oxidoreductase activity
E	0045271	cellular_component	respiratory chain complex I
E	0046872	molecular_function	metal ion binding
E	0051537	molecular_function	2 iron, 2 sulfur cluster binding
E	1902600	biological_process	proton transmembrane transport
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
F	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
F	0016491	molecular_function	oxidoreductase activity
F	0045271	cellular_component	respiratory chain complex I
F	0046872	molecular_function	metal ion binding
F	0051539	molecular_function	4 iron, 4 sulfur cluster binding
F	1902600	biological_process	proton transmembrane transport
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005758	cellular_component	mitochondrial intermembrane space
G	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
G	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
G	0009055	molecular_function	electron transfer activity
G	0016491	molecular_function	oxidoreductase activity
G	0032981	biological_process	mitochondrial respiratory chain complex I assembly
G	0045271	cellular_component	respiratory chain complex I
G	0045333	biological_process	cellular respiration
G	0046872	molecular_function	metal ion binding
G	0051537	molecular_function	2 iron, 2 sulfur cluster binding
G	0051539	molecular_function	4 iron, 4 sulfur cluster binding
G	1902600	biological_process	proton transmembrane transport
H	0003954	molecular_function	NADH dehydrogenase activity
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
H	0009060	biological_process	aerobic respiration
H	0032981	biological_process	mitochondrial respiratory chain complex I assembly
H	0045271	cellular_component	respiratory chain complex I
H	1902600	biological_process	proton transmembrane transport
I	0003954	molecular_function	NADH dehydrogenase activity
I	0005739	cellular_component	mitochondrion
I	0005743	cellular_component	mitochondrial inner membrane
I	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
I	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
I	0016491	molecular_function	oxidoreductase activity
I	0032981	biological_process	mitochondrial respiratory chain complex I assembly
I	0045271	cellular_component	respiratory chain complex I
I	0046872	molecular_function	metal ion binding
I	0051539	molecular_function	4 iron, 4 sulfur cluster binding
I	1902600	biological_process	proton transmembrane transport
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
J	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
J	0032981	biological_process	mitochondrial respiratory chain complex I assembly
J	1902600	biological_process	proton transmembrane transport
K	0005739	cellular_component	mitochondrion
K	0005743	cellular_component	mitochondrial inner membrane
K	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
K	0045271	cellular_component	respiratory chain complex I
K	1902600	biological_process	proton transmembrane transport
L	0003954	molecular_function	NADH dehydrogenase activity
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
L	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
L	0015990	biological_process	electron transport coupled proton transport
L	0032981	biological_process	mitochondrial respiratory chain complex I assembly
L	0045271	cellular_component	respiratory chain complex I
M	0003954	molecular_function	NADH dehydrogenase activity
M	0005739	cellular_component	mitochondrion
M	0005743	cellular_component	mitochondrial inner membrane
M	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
M	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
M	0009060	biological_process	aerobic respiration
M	0015990	biological_process	electron transport coupled proton transport
M	0032981	biological_process	mitochondrial respiratory chain complex I assembly
M	0045271	cellular_component	respiratory chain complex I
M	0048039	molecular_function	ubiquinone binding
N	0005739	cellular_component	mitochondrion
N	0005743	cellular_component	mitochondrial inner membrane
N	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
N	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
N	0032981	biological_process	mitochondrial respiratory chain complex I assembly
N	0045271	cellular_component	respiratory chain complex I
N	1902600	biological_process	proton transmembrane transport
O	0005737	cellular_component	cytoplasm
O	0005739	cellular_component	mitochondrion
O	0005759	cellular_component	mitochondrial matrix
O	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
O	0032981	biological_process	mitochondrial respiratory chain complex I assembly
O	0045271	cellular_component	respiratory chain complex I
P	0005739	cellular_component	mitochondrion
P	0005759	cellular_component	mitochondrial matrix
P	0032981	biological_process	mitochondrial respiratory chain complex I assembly
P	0044877	molecular_function	protein-containing complex binding
P	0045271	cellular_component	respiratory chain complex I
P	1901006	biological_process	ubiquinone-6 biosynthetic process
Q	0001932	biological_process	regulation of protein phosphorylation
Q	0005739	cellular_component	mitochondrion
Q	0005743	cellular_component	mitochondrial inner membrane
Q	0007420	biological_process	brain development
Q	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
Q	0032981	biological_process	mitochondrial respiratory chain complex I assembly
Q	0045271	cellular_component	respiratory chain complex I
Q	0051591	biological_process	response to cAMP
Q	0072593	biological_process	reactive oxygen species metabolic process
Q	1902600	biological_process	proton transmembrane transport
R	0005739	cellular_component	mitochondrion
R	0005743	cellular_component	mitochondrial inner membrane
R	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
R	0045271	cellular_component	respiratory chain complex I
S	0005739	cellular_component	mitochondrion
S	0005743	cellular_component	mitochondrial inner membrane
S	0031966	cellular_component	mitochondrial membrane
S	0032981	biological_process	mitochondrial respiratory chain complex I assembly
S	0045271	cellular_component	respiratory chain complex I
T	0000035	molecular_function	acyl binding
T	0000036	molecular_function	acyl carrier activity
T	0005504	molecular_function	fatty acid binding
T	0005739	cellular_component	mitochondrion
T	0005759	cellular_component	mitochondrial matrix
T	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
T	0006633	biological_process	fatty acid biosynthetic process
T	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
T	0016491	molecular_function	oxidoreductase activity
T	0031966	cellular_component	mitochondrial membrane
T	0032981	biological_process	mitochondrial respiratory chain complex I assembly
T	0044571	biological_process	[2Fe-2S] cluster assembly
T	0045271	cellular_component	respiratory chain complex I
T	0140978	molecular_function	mitochondrial large ribosomal subunit binding
T	1902494	cellular_component	catalytic complex
T	1902600	biological_process	proton transmembrane transport
U	0000035	molecular_function	acyl binding
U	0000036	molecular_function	acyl carrier activity
U	0005504	molecular_function	fatty acid binding
U	0005739	cellular_component	mitochondrion
U	0005759	cellular_component	mitochondrial matrix
U	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
U	0006633	biological_process	fatty acid biosynthetic process
U	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
U	0016491	molecular_function	oxidoreductase activity
U	0031966	cellular_component	mitochondrial membrane
U	0032981	biological_process	mitochondrial respiratory chain complex I assembly
U	0044571	biological_process	[2Fe-2S] cluster assembly
U	0045271	cellular_component	respiratory chain complex I
U	0140978	molecular_function	mitochondrial large ribosomal subunit binding
U	1902494	cellular_component	catalytic complex
U	1902600	biological_process	proton transmembrane transport
V	0005739	cellular_component	mitochondrion
V	0005743	cellular_component	mitochondrial inner membrane
V	0022904	biological_process	respiratory electron transport chain
V	0032981	biological_process	mitochondrial respiratory chain complex I assembly
V	0045271	cellular_component	respiratory chain complex I
W	0005739	cellular_component	mitochondrion
W	0005743	cellular_component	mitochondrial inner membrane
W	0006979	biological_process	response to oxidative stress
W	0032981	biological_process	mitochondrial respiratory chain complex I assembly
W	0045271	cellular_component	respiratory chain complex I
X	0005739	cellular_component	mitochondrion
X	0005743	cellular_component	mitochondrial inner membrane
X	0005758	cellular_component	mitochondrial intermembrane space
X	0032981	biological_process	mitochondrial respiratory chain complex I assembly
X	0045271	cellular_component	respiratory chain complex I
Y	0005739	cellular_component	mitochondrion
Y	0005743	cellular_component	mitochondrial inner membrane
Y	0032981	biological_process	mitochondrial respiratory chain complex I assembly
Y	0045271	cellular_component	respiratory chain complex I
Z	0005634	cellular_component	nucleus
Z	0005654	cellular_component	nucleoplasm
Z	0005737	cellular_component	cytoplasm
Z	0005739	cellular_component	mitochondrion
Z	0005743	cellular_component	mitochondrial inner membrane
Z	0006915	biological_process	apoptotic process
Z	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
Z	0032981	biological_process	mitochondrial respiratory chain complex I assembly
Z	0045271	cellular_component	respiratory chain complex I
Z	0045892	biological_process	negative regulation of DNA-templated transcription
Z	1902600	biological_process	proton transmembrane transport
a	0005739	cellular_component	mitochondrion
a	0005743	cellular_component	mitochondrial inner membrane
a	0032981	biological_process	mitochondrial respiratory chain complex I assembly
a	0045271	cellular_component	respiratory chain complex I
b	0005739	cellular_component	mitochondrion
b	0005743	cellular_component	mitochondrial inner membrane
b	0032981	biological_process	mitochondrial respiratory chain complex I assembly
b	0045271	cellular_component	respiratory chain complex I
c	0005739	cellular_component	mitochondrion
c	0005743	cellular_component	mitochondrial inner membrane
c	0032981	biological_process	mitochondrial respiratory chain complex I assembly
c	0045271	cellular_component	respiratory chain complex I
d	0005739	cellular_component	mitochondrion
d	0005743	cellular_component	mitochondrial inner membrane
d	0032981	biological_process	mitochondrial respiratory chain complex I assembly
d	0045271	cellular_component	respiratory chain complex I
e	0005739	cellular_component	mitochondrion
e	0005743	cellular_component	mitochondrial inner membrane
e	0005758	cellular_component	mitochondrial intermembrane space
e	0032981	biological_process	mitochondrial respiratory chain complex I assembly
e	0045271	cellular_component	respiratory chain complex I
f	0005739	cellular_component	mitochondrion
f	0005743	cellular_component	mitochondrial inner membrane
f	0032981	biological_process	mitochondrial respiratory chain complex I assembly
f	0045271	cellular_component	respiratory chain complex I
g	0005739	cellular_component	mitochondrion
g	0005743	cellular_component	mitochondrial inner membrane
g	0032981	biological_process	mitochondrial respiratory chain complex I assembly
g	0045271	cellular_component	respiratory chain complex I
h	0005739	cellular_component	mitochondrion
h	0005743	cellular_component	mitochondrial inner membrane
h	0032981	biological_process	mitochondrial respiratory chain complex I assembly
h	0045271	cellular_component	respiratory chain complex I
i	0005654	cellular_component	nucleoplasm
i	0005739	cellular_component	mitochondrion
i	0005743	cellular_component	mitochondrial inner membrane
i	0031966	cellular_component	mitochondrial membrane
i	0032981	biological_process	mitochondrial respiratory chain complex I assembly
i	0042775	biological_process	mitochondrial ATP synthesis coupled electron transport
i	0045271	cellular_component	respiratory chain complex I
j	0005739	cellular_component	mitochondrion
j	0005743	cellular_component	mitochondrial inner membrane
j	0032981	biological_process	mitochondrial respiratory chain complex I assembly
j	0045271	cellular_component	respiratory chain complex I
k	0005739	cellular_component	mitochondrion
k	0005743	cellular_component	mitochondrial inner membrane
k	0032981	biological_process	mitochondrial respiratory chain complex I assembly
k	0045271	cellular_component	respiratory chain complex I
l	0005739	cellular_component	mitochondrion
l	0005743	cellular_component	mitochondrial inner membrane
l	0032981	biological_process	mitochondrial respiratory chain complex I assembly
l	0045271	cellular_component	respiratory chain complex I
m	0005739	cellular_component	mitochondrion
m	0005743	cellular_component	mitochondrial inner membrane
m	0032981	biological_process	mitochondrial respiratory chain complex I assembly
m	0045271	cellular_component	respiratory chain complex I
n	0005739	cellular_component	mitochondrion
n	0005743	cellular_component	mitochondrial inner membrane
n	0032981	biological_process	mitochondrial respiratory chain complex I assembly
n	0045271	cellular_component	respiratory chain complex I
o	0005739	cellular_component	mitochondrion
o	0005743	cellular_component	mitochondrial inner membrane
o	0005758	cellular_component	mitochondrial intermembrane space
o	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
o	0032981	biological_process	mitochondrial respiratory chain complex I assembly
o	0045271	cellular_component	respiratory chain complex I
o	1902600	biological_process	proton transmembrane transport
p	0005739	cellular_component	mitochondrion
p	0005743	cellular_component	mitochondrial inner membrane
p	0032981	biological_process	mitochondrial respiratory chain complex I assembly
p	0045271	cellular_component	respiratory chain complex I
q	0005739	cellular_component	mitochondrion
q	0005743	cellular_component	mitochondrial inner membrane
q	0006979	biological_process	response to oxidative stress
q	0045271	cellular_component	respiratory chain complex I
r	0005739	cellular_component	mitochondrion
r	0005743	cellular_component	mitochondrial inner membrane
r	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
r	0016491	molecular_function	oxidoreductase activity
r	0042773	biological_process	ATP synthesis coupled electron transport
r	0045271	cellular_component	respiratory chain complex I
s	0005739	cellular_component	mitochondrion
s	0005743	cellular_component	mitochondrial inner membrane
s	0042775	biological_process	mitochondrial ATP synthesis coupled electron transport
s	0045271	cellular_component	respiratory chain complex I

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGLDSLDQVEIIMAM

Chain	Residue	Details
T	ASP39-MET54

---

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DIDAEKLMCpqEI

Chain	Residue	Details
T	ASP64-ILE76

---

site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiACKlCEaVCP

Chain	Residue	Details
I	CYS77-PRO88
I	CYS116-PRO127

---

site_id	PS00535
Number of Residues	12
Details	COMPLEX1_49K Respiratory chain NADH dehydrogenase 49 Kd subunit signature. LHRGtEKLiEyK

Chain	Residue	Details
D	LEU83-LYS94

---

site_id	PS00542
Number of Residues	22
Details	COMPLEX1_30K Respiratory chain NADH dehydrogenase 30 Kd subunit signature. EREiwDMFgvffanHpdlRrIL

Chain	Residue	Details
C	GLU131-LEU152

---

site_id	PS00641
Number of Residues	18
Details	COMPLEX1_75K_1 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. PrfCYherlsvaGnCRmC

Chain	Residue	Details
G	PRO38-CYS55

---

site_id	PS00642
Number of Residues	13
Details	COMPLEX1_75K_2 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. CPiCDqGGeCdLQ

Chain	Residue	Details
G	CYS105-GLN117

---

site_id	PS00643
Number of Residues	11
Details	COMPLEX1_75K_3 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. RCIqCtRCIrF

Chain	Residue	Details
G	ARG152-PHE162

---

site_id	PS00644
Number of Residues	16
Details	COMPLEX1_51K_1 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. GAGAYICGEETALIES

Chain	Residue	Details
F	GLY180-SER195

---

site_id	PS00645
Number of Residues	12
Details	COMPLEX1_51K_2 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. ESCGqCtPCReG

Chain	Residue	Details
F	GLU357-GLY368

---

site_id	PS00667
Number of Residues	16
Details	COMPLEX1_ND1_1 Respiratory-chain NADH dehydrogenase subunit 1 signature 1. GLLQpIaDAIKLFiKE

Chain	Residue	Details
H	GLY44-GLU59

---

site_id	PS00668
Number of Residues	14
Details	COMPLEX1_ND1_2 Respiratory-chain NADH dehydrogenase subunit 1 signature 2. PFDLTEGEseLVs.G

Chain	Residue	Details
H	PRO197-GLY210

---

site_id	PS01099
Number of Residues	19
Details	COMPLEX1_24K Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. DklFTlieveCLGaCvnAP

Chain	Residue	Details
E	ASP134-PRO152

---

site_id	PS01150
Number of Residues	17
Details	COMPLEX1_20K Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. GcDRIVPVDIYvPgCPP

Chain	Residue	Details
B	GLY135-PRO151

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	60
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
A	LEU3-TRP23
N	ASN274-MET294
N	PHE325-VAL345
M	ILE393-MET413
M	ALA435-LEU455
L	MET482-LEU502
L	GLY582-PHE602
A	PHE55-LEU75
A	LEU84-TYR104
I	CYS87
I	CYS116
I	CYS119
I	CYS122
I	CYS126
N	ILE239-GLY259

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-formylmethionine => ECO:0000269|PubMed:17060615

Chain	Residue	Details
A	FME1
G	CYS105
G	CYS108
G	CYS114

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q91VD9

Chain	Residue	Details
G	LYS61
G	LYS476
G	LYS686

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q91YT0

Chain	Residue	Details
F	LYS84
F	LYS355

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:Q91YT0

Chain	Residue	Details
F	ARG237

---