8OVW

Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
A	0001227	molecular_function	DNA-binding transcription repressor activity, RNA polymerase II-specific
A	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
A	0003677	molecular_function	DNA binding
A	0003700	molecular_function	DNA-binding transcription factor activity
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0005739	cellular_component	mitochondrion
A	0006338	biological_process	chromatin remodeling
A	0006357	biological_process	regulation of transcription by RNA polymerase II
A	0007059	biological_process	chromosome segregation
A	0019237	molecular_function	centromeric DNA binding
A	0042762	biological_process	regulation of sulfur metabolic process
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0046983	molecular_function	protein dimerization activity
A	0061431	biological_process	cellular response to methionine
A	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
A	0089713	cellular_component	Cbf1-Met4-Met28 complex
A	1900059	biological_process	positive regulation of sulfate assimilation
A	1900060	biological_process	negative regulation of ceramide biosynthetic process
A	1900090	biological_process	positive regulation of inositol biosynthetic process
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000775	cellular_component	chromosome, centromeric region
B	0000776	cellular_component	kinetochore
B	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
B	0001227	molecular_function	DNA-binding transcription repressor activity, RNA polymerase II-specific
B	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
B	0003677	molecular_function	DNA binding
B	0003700	molecular_function	DNA-binding transcription factor activity
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0005739	cellular_component	mitochondrion
B	0006338	biological_process	chromatin remodeling
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0007059	biological_process	chromosome segregation
B	0019237	molecular_function	centromeric DNA binding
B	0042762	biological_process	regulation of sulfur metabolic process
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0046983	molecular_function	protein dimerization activity
B	0061431	biological_process	cellular response to methionine
B	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
B	0089713	cellular_component	Cbf1-Met4-Met28 complex
B	1900059	biological_process	positive regulation of sulfate assimilation
B	1900060	biological_process	negative regulation of ceramide biosynthetic process
B	1900090	biological_process	positive regulation of inositol biosynthetic process
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0003674	molecular_function	molecular_function
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005694	cellular_component	chromosome
H	0007059	biological_process	chromosome segregation
H	0008608	biological_process	attachment of spindle microtubules to kinetochore
H	0034087	biological_process	establishment of mitotic sister chromatid cohesion
H	0051301	biological_process	cell division
H	0051321	biological_process	meiotic cell cycle
I	0000775	cellular_component	chromosome, centromeric region
I	0000776	cellular_component	kinetochore
I	0003674	molecular_function	molecular_function
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005694	cellular_component	chromosome
I	0006270	biological_process	DNA replication initiation
I	0007059	biological_process	chromosome segregation
I	0007094	biological_process	mitotic spindle assembly checkpoint signaling
I	0008608	biological_process	attachment of spindle microtubules to kinetochore
I	0034087	biological_process	establishment of mitotic sister chromatid cohesion
I	0051301	biological_process	cell division
I	0051321	biological_process	meiotic cell cycle
K	0000775	cellular_component	chromosome, centromeric region
K	0000776	cellular_component	kinetochore
K	0003674	molecular_function	molecular_function
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005694	cellular_component	chromosome
K	0007059	biological_process	chromosome segregation
K	0008608	biological_process	attachment of spindle microtubules to kinetochore
K	0034087	biological_process	establishment of mitotic sister chromatid cohesion
K	0051301	biological_process	cell division
K	0051321	biological_process	meiotic cell cycle
L	0000775	cellular_component	chromosome, centromeric region
L	0000776	cellular_component	kinetochore
L	0000940	cellular_component	outer kinetochore
L	0003674	molecular_function	molecular_function
L	0005634	cellular_component	nucleus
L	0005694	cellular_component	chromosome
L	0008608	biological_process	attachment of spindle microtubules to kinetochore
L	0030437	biological_process	ascospore formation
L	0034087	biological_process	establishment of mitotic sister chromatid cohesion
L	0034089	biological_process	establishment of meiotic sister chromatid cohesion
L	0034090	biological_process	maintenance of meiotic sister chromatid cohesion
L	0045144	biological_process	meiotic sister chromatid segregation
L	0051301	biological_process	cell division
L	0051321	biological_process	meiotic cell cycle
L	0071459	biological_process	protein localization to chromosome, centromeric region
N	0000775	cellular_component	chromosome, centromeric region
N	0000776	cellular_component	kinetochore
N	0000940	cellular_component	outer kinetochore
N	0005198	molecular_function	structural molecule activity
N	0005634	cellular_component	nucleus
N	0005694	cellular_component	chromosome
N	0007059	biological_process	chromosome segregation
N	0007094	biological_process	mitotic spindle assembly checkpoint signaling
N	0008608	biological_process	attachment of spindle microtubules to kinetochore
N	0034080	biological_process	CENP-A containing chromatin assembly
N	0034087	biological_process	establishment of mitotic sister chromatid cohesion
N	0034089	biological_process	establishment of meiotic sister chromatid cohesion
N	0034090	biological_process	maintenance of meiotic sister chromatid cohesion
N	0051301	biological_process	cell division
N	0051321	biological_process	meiotic cell cycle
N	0051382	biological_process	kinetochore assembly
N	0071459	biological_process	protein localization to chromosome, centromeric region
O	0000775	cellular_component	chromosome, centromeric region
O	0000776	cellular_component	kinetochore
O	0000817	cellular_component	COMA complex
O	0003674	molecular_function	molecular_function
O	0005634	cellular_component	nucleus
O	0005694	cellular_component	chromosome
O	0008608	biological_process	attachment of spindle microtubules to kinetochore
O	0034087	biological_process	establishment of mitotic sister chromatid cohesion
O	0034508	biological_process	centromere complex assembly
O	0051301	biological_process	cell division
O	0051321	biological_process	meiotic cell cycle
O	0071459	biological_process	protein localization to chromosome, centromeric region
O	1905262	biological_process	negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination
P	0000775	cellular_component	chromosome, centromeric region
P	0000776	cellular_component	kinetochore
P	0000817	cellular_component	COMA complex
P	0003674	molecular_function	molecular_function
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005694	cellular_component	chromosome
P	0007059	biological_process	chromosome segregation
P	0007094	biological_process	mitotic spindle assembly checkpoint signaling
P	0008608	biological_process	attachment of spindle microtubules to kinetochore
P	0034087	biological_process	establishment of mitotic sister chromatid cohesion
P	0051301	biological_process	cell division
P	0051321	biological_process	meiotic cell cycle
P	0071459	biological_process	protein localization to chromosome, centromeric region
Q	0000775	cellular_component	chromosome, centromeric region
Q	0000776	cellular_component	kinetochore
Q	0000817	cellular_component	COMA complex
Q	0003674	molecular_function	molecular_function
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005694	cellular_component	chromosome
Q	0008608	biological_process	attachment of spindle microtubules to kinetochore
Q	0051301	biological_process	cell division
Q	0051321	biological_process	meiotic cell cycle
T	0000775	cellular_component	chromosome, centromeric region
T	0000776	cellular_component	kinetochore
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005694	cellular_component	chromosome
T	0007059	biological_process	chromosome segregation
T	0019237	molecular_function	centromeric DNA binding
T	0044877	molecular_function	protein-containing complex binding
T	0051301	biological_process	cell division
T	1905560	biological_process	negative regulation of kinetochore assembly
U	0000775	cellular_component	chromosome, centromeric region
U	0000776	cellular_component	kinetochore
U	0000817	cellular_component	COMA complex
U	0003674	molecular_function	molecular_function
U	0005634	cellular_component	nucleus
U	0005694	cellular_component	chromosome
U	0005737	cellular_component	cytoplasm
U	0005816	cellular_component	spindle pole body
U	0008608	biological_process	attachment of spindle microtubules to kinetochore
U	0034501	biological_process	protein localization to kinetochore
U	0051301	biological_process	cell division
U	0051321	biological_process	meiotic cell cycle
W	0000775	cellular_component	chromosome, centromeric region
W	0000776	cellular_component	kinetochore
W	0005634	cellular_component	nucleus
W	0005694	cellular_component	chromosome
W	0007059	biological_process	chromosome segregation
W	0019237	molecular_function	centromeric DNA binding
Y	0000775	cellular_component	chromosome, centromeric region
Y	0000776	cellular_component	kinetochore
Y	0003674	molecular_function	molecular_function
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005694	cellular_component	chromosome
Y	0008150	biological_process	biological_process
Y	0008608	biological_process	attachment of spindle microtubules to kinetochore
Y	0051301	biological_process	cell division
Y	0051321	biological_process	meiotic cell cycle
Z	0000775	cellular_component	chromosome, centromeric region
Z	0000776	cellular_component	kinetochore
Z	0003674	molecular_function	molecular_function
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005694	cellular_component	chromosome
Z	0007059	biological_process	chromosome segregation
Z	0008608	biological_process	attachment of spindle microtubules to kinetochore
Z	0031511	cellular_component	Mis6-Sim4 complex
Z	0051301	biological_process	cell division
Z	0051321	biological_process	meiotic cell cycle

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
O	THR88
T	SER52
T	SER55
T	SER115
T	SER177
T	SER192
T	SER268

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site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
U	SER45
T	THR91
T	THR129
T	THR139

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
U	SER53
T	SER50
T	SER74

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site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
U	SER59
U	SER101
T	THR191

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site_id	SWS_FT_FI5
Number of Residues	5
Details	MOD_RES: Phosphothreonine; by MPS1 => ECO:0000269|PubMed:23334295

Chain	Residue	Details
T	THR53
T	THR86
T	THR88
T	THR134
T	THR174

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site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine; by MPS1 => ECO:0000269|PubMed:23334295

Chain	Residue	Details
T	SER135
T	SER153

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