Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8ORE

Cryo-EM structure of SH-SY5Y seeded with filaments from Alzheimer's Disease

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008017	molecular_function	microtubule binding
A	0015631	molecular_function	tubulin binding
B	0008017	molecular_function	microtubule binding
B	0015631	molecular_function	tubulin binding
C	0008017	molecular_function	microtubule binding
C	0015631	molecular_function	tubulin binding

Functional Information from PROSITE/UniProt

site_id	PS00229
Number of Residues	13
Details	TAU_MAP_1 Tau and MAP proteins tubulin-binding repeat signature. GSteNlkHqPGGG

Chain	Residue	Details
A	GLY261-GLY273
A	GLY323-GLY335
A	GLY355-GLY367

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	84
Details	SITE: Not glycated => ECO:0000269\|PubMed:9326300

Chain	Residue	Details
A	LYS274
A	LYS321
A	LEU325
A	HIS329
A	GLU342
A	ASP348
A	SER352
A	HIS362
A	ILE371
A	HIS374
A	GLY401
A	ARG406
A	THR414
A	SER416
A	ALA426
B	LYS53
B	LYS73
B	LYS96
B	LEU441
B	LYS148
B	LYS180
B	LYS190
B	LYS224
B	LYS240
B	LYS254
B	LYS257
B	LYS267
B	LYS274
B	LYS321
B	LEU325
B	HIS329
B	GLU342
B	ASP348
B	SER352
B	HIS362
B	ILE371
B	HIS374
B	GLY401
B	ARG406
B	THR414
B	SER416
B	ALA426
C	LYS53
C	LYS73
C	LYS96
C	LEU441
C	LYS148
C	LYS180
C	LYS190
C	LYS224
C	LYS240
C	LYS254
C	LYS257
C	LYS267
C	LYS274
C	LYS321
C	LEU325
C	HIS329
C	GLU342
C	ASP348
C	SER352
C	HIS362
C	ILE371
C	HIS374
C	GLY401
C	ARG406
C	THR414
C	SER416
C	ALA426
A	LYS254
A	LYS257
A	LYS148
A	LYS180
A	LYS190
A	LYS224
A	LYS240
A	LYS267
A	LYS53
A	LYS73
A	LYS96
A	LEU441

site_id	SWS_FT_FI2
Number of Residues	3
Details	MOD_RES: N-acetylalanine => ECO:0000269\|PubMed:1512244

Chain	Residue	Details
A	ALA31
B	ALA31
C	ALA31

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: Phosphotyrosine; by FYN => ECO:0000269\|PubMed:14999081

Chain	Residue	Details
A	TYR47
B	TYR47
C	TYR47

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
B	LEU425
C	TYR58
C	LEU425
A	LEU425
B	TYR58
A	TYR58

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P19332

Chain	Residue	Details
A	SER75
A	SER90
B	SER75
B	SER90
C	SER75
C	SER90

site_id	SWS_FT_FI6
Number of Residues	15
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
B	LYS140
B	THR169
B	THR175
B	ALA434
C	THR98
C	LYS140
C	THR169
C	THR175
C	ALA434
A	THR169
A	THR175
A	ALA434
B	THR98
A	THR98
A	LYS140

site_id	SWS_FT_FI7
Number of Residues	3
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P19332

Chain	Residue	Details
A	THR100
B	THR100
C	THR100

site_id	SWS_FT_FI8
Number of Residues	3
Details	MOD_RES: Phosphoserine; by SGK1 => ECO:0000269\|PubMed:16982696

Chain	Residue	Details
A	LEU243
B	LEU243
C	LEU243

site_id	SWS_FT_FI9
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269\|PubMed:9614189

Chain	Residue	Details
A	THR153
B	THR153
C	THR153

site_id	SWS_FT_FI10
Number of Residues	6
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	GLU380
B	GLU380
C	GLU380
A	ARG155
B	ARG155
C	ARG155

site_id	SWS_FT_FI11
Number of Residues	33
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	LYS163
A	PRO312
A	HIS329
A	GLU342
A	ASP348
A	SER352
A	HIS362
A	HIS374
A	PHE378
A	SER400
A	SER416
B	LYS163
B	PRO312
B	HIS329
B	GLU342
B	ASP348
B	SER352
B	HIS362
B	HIS374
B	PHE378
B	SER400
B	SER416
C	LYS163
C	PRO312
C	HIS329
C	GLU342
C	ASP348
C	SER352
C	HIS362
C	HIS374
C	PHE378
C	SER400
C	SER416

site_id	SWS_FT_FI12
Number of Residues	6
Details	MOD_RES: Deamidated asparagine; in tau and PHF-tau; partial => ECO:0000269\|PubMed:1512244

Chain	Residue	Details
A	ASN167
A	TYR310
B	ASN167
B	TYR310
C	ASN167
C	TYR310

site_id	SWS_FT_FI13
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269\|PubMed:15546861

Chain	Residue	Details
A	THR181
B	THR181
C	THR181

site_id	SWS_FT_FI14
Number of Residues	9
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	SER185
A	SER191
A	SER195
B	SER185
B	SER191
B	SER195
C	SER185
C	SER191
C	SER195

site_id	SWS_FT_FI15
Number of Residues	3
Details	MOD_RES: Phosphotyrosine; by TTBK1 => ECO:0000269\|PubMed:16923168

Chain	Residue	Details
A	TYR197
B	TYR197
C	TYR197

site_id	SWS_FT_FI16
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PDPK1 and TTBK1 => ECO:0000269\|PubMed:16923168

Chain	Residue	Details
A	SER198
B	SER198
C	SER198

site_id	SWS_FT_FI17
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PDPK1 and TTBK1 => ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16923168, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:9614189

Chain	Residue	Details
A	SER199
B	SER199
C	SER199

site_id	SWS_FT_FI18
Number of Residues	3
Details	MOD_RES: Phosphoserine; by CK1, PDPK1 and TTBK1 => ECO:0000269\|PubMed:14761950, ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16923168, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:21327254, ECO:0000269\|PubMed:9614189, ECO:0007744\|PubMed:18220336, ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
A	SER202
B	SER202
C	SER202

site_id	SWS_FT_FI19
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by CK1 and PDPK1 => ECO:0000269\|PubMed:14761950, ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:19451179

Chain	Residue	Details
A	THR205
B	THR205
C	THR205

site_id	SWS_FT_FI20
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by BRSK1, BRSK2, DYRK2 and PDPK1 => ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:18599021, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:21985311, ECO:0000269\|PubMed:9614189

Chain	Residue	Details
A	THR212
B	THR212
C	THR212

site_id	SWS_FT_FI21
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:9614189

Chain	Residue	Details
A	SER214
B	SER214
C	SER214

site_id	SWS_FT_FI22
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:19451179

Chain	Residue	Details
A	THR217
B	THR217
C	THR217

site_id	SWS_FT_FI23
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	LYS225
A	LYS321
B	LYS225
B	LYS321
C	LYS225
C	LYS321

site_id	SWS_FT_FI24
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by GSK3-beta and PDPK1 => ECO:0000269\|PubMed:14690523, ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16443603, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	THR231
B	THR231
C	THR231

site_id	SWS_FT_FI25
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PDPK1 => ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:9614189, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER235
B	SER235
C	SER235

site_id	SWS_FT_FI26
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PHK => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:8999860

Chain	Residue	Details
A	SER237
B	SER237
C	SER237

site_id	SWS_FT_FI27
Number of Residues	3
Details	MOD_RES: N6-methyllysine; alternate => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	LYS259
B	LYS259
C	LYS259

site_id	SWS_FT_FI28
Number of Residues	3
Details	MOD_RES: Phosphoserine; by MARK1, MARK2, MARK3, MARK4, BRSK1, BRSK2 and PHK => ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:21985311, ECO:0000269\|PubMed:23666762, ECO:0000269\|PubMed:7706316, ECO:0000269\|PubMed:8999860, ECO:0000269\|PubMed:9614189

Chain	Residue	Details
A	SER262
B	SER262
C	SER262

site_id	SWS_FT_FI29
Number of Residues	6
Details	MOD_RES: Phosphoserine; by PHK => ECO:0000269\|PubMed:8999860

Chain	Residue	Details
A	SER316
A	LYS383
B	SER316
B	LYS383
C	SER316
C	LYS383

site_id	SWS_FT_FI30
Number of Residues	9
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:7706316

Chain	Residue	Details
A	SER324
A	GLY355
A	ASP387
B	SER324
B	GLY355
B	ASP387
C	SER324
C	GLY355
C	ASP387

site_id	SWS_FT_FI31
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PHK => ECO:0000269\|PubMed:7706316, ECO:0000269\|PubMed:8999860

Chain	Residue	Details
A	GLN336
B	GLN336
C	GLN336

site_id	SWS_FT_FI32
Number of Residues	3
Details	MOD_RES: Phosphoserine; by CK1 and PDPK1 => ECO:0000269\|PubMed:14761950, ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:21327254, ECO:0000269\|PubMed:9614189, ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	THR427
B	THR427
C	THR427

site_id	SWS_FT_FI33
Number of Residues	3
Details	MOD_RES: Phosphoserine; alternate => ECO:0007744\|PubMed:19690332

Chain	Residue	Details
A	GLU431
B	GLU431
C	GLU431

site_id	SWS_FT_FI34
Number of Residues	3
Details	MOD_RES: Phosphoserine; by CK1 and PDPK1 => ECO:0000269\|PubMed:14761950, ECO:0000269\|PubMed:15546861, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:21327254, ECO:0000269\|PubMed:9614189, ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER435
B	SER435
C	SER435

site_id	SWS_FT_FI35
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:15546861, ECO:0007744\|PubMed:19690332

Chain	Residue	Details
A	GLY440
B	GLY440
C	GLY440

site_id	SWS_FT_FI36
Number of Residues	3
Details	MOD_RES: Phosphoserine; by CaMK2 and TTBK1 => ECO:0000269\|PubMed:16923168

Chain

Residue

Details

site_id	SWS_FT_FI37
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PDPK1 and TTBK1 => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16923168, ECO:0000269\|PubMed:19451179, ECO:0000269\|PubMed:9614189

Chain

Residue

Details

site_id	SWS_FT_FI38
Number of Residues	3
Details	MOD_RES: Phosphothreonine; by TTBK1 => ECO:0000269\|PubMed:16923168

Chain

Residue

Details

site_id	SWS_FT_FI39
Number of Residues	36
Details	CARBOHYD: N-linked (Glc) (glycation) lysine; in PHF-tau; in vitro => ECO:0000269\|PubMed:9326300

Chain	Residue	Details
A	ASP116
A	LYS150
A	LYS163
A	LYS174
A	LYS225
A	LYS234
A	LYS259
A	LYS311
A	PRO312
A	PHE378
A	ALA384
A	SER400
B	ASP116
B	LYS150
B	LYS163
B	LYS174
B	LYS225
B	LYS234
B	LYS259
B	LYS311
B	PRO312
B	PHE378
B	ALA384
B	SER400
C	ASP116
C	LYS150
C	LYS163
C	LYS174
C	LYS225
C	LYS234
C	LYS259
C	LYS311
C	PRO312
C	PHE378
C	ALA384
C	SER400

site_id	SWS_FT_FI40
Number of Residues	6
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000269\|PubMed:21327254

Chain	Residue	Details
A	SER208
A	SER238
B	SER208
B	SER238
C	SER208
C	SER238

site_id	SWS_FT_FI41
Number of Residues	3
Details	CARBOHYD: O-linked (GlcNAc) serine; alternate => ECO:0000269\|PubMed:21327254

Chain	Residue	Details
A	GLU431
B	GLU431
C	GLU431

site_id	SWS_FT_FI42
Number of Residues	9
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	LYS73
A	LYS267
A	ARG406
B	LYS73
B	LYS267
B	ARG406
C	LYS73
C	LYS267
C	ARG406

site_id	SWS_FT_FI43
Number of Residues	33
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250\|UniProtKB:P10637

Chain	Residue	Details
A	LYS259
A	PRO312
A	HIS329
A	ASP348
A	SER352
A	HIS362
A	HIS374
A	PHE378
A	SER400
A	SER416
B	LYS259
B	PRO312
B	HIS329
B	ASP348
B	SER352
B	HIS362
B	HIS374
B	PHE378
B	SER400
B	SER416
C	LYS259
C	PRO312
C	HIS329
C	ASP348
C	SER352
C	HIS362
C	HIS374
C	PHE378
C	SER400
C	SER416

site_id	SWS_FT_FI44
Number of Residues	9
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); in PHF-tau => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
A	LYS254
A	GLU342
A	ALA384
B	LYS254
B	GLU342
B	ALA384
C	LYS254
C	GLU342
C	ALA384

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)