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8KAH

Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 18nt target DNA

Functional Information from GO Data
ChainGOidnamespacecontents
B0003677molecular_functionDNA binding
B0003723molecular_functionRNA binding
B0004519molecular_functionendonuclease activity
B0004520molecular_functionDNA endonuclease activity
B0004527molecular_functionexonuclease activity
B0008408molecular_function3'-5' exonuclease activity
B0043571biological_processmaintenance of CRISPR repeat elements
B0046872molecular_functionmetal ion binding
B0051607biological_processdefense response to virus
F0003677molecular_functionDNA binding
F0003723molecular_functionRNA binding
F0004519molecular_functionendonuclease activity
F0004520molecular_functionDNA endonuclease activity
F0004527molecular_functionexonuclease activity
F0008408molecular_function3'-5' exonuclease activity
F0043571biological_processmaintenance of CRISPR repeat elements
F0046872molecular_functionmetal ion binding
F0051607biological_processdefense response to virus
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477
ChainResidueDetails
BALA10
FALA10

site_idSWS_FT_FI2
Number of Residues2
DetailsACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477
ChainResidueDetails
BALA840
FALA840

site_idSWS_FT_FI3
Number of Residues12
DetailsBINDING: BINDING => ECO:0000269|PubMed:24505130
ChainResidueDetails
BALA10
FHIS983
FHIS1297
FASP1328
BGLU762
BGLU766
BHIS983
BHIS1297
BASP1328
FALA10
FGLU762
FGLU766

227344

PDB entries from 2024-11-13

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