8KAH
Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 18nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.214, 130.605, 148.734 |
| Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
| Resolution | 48.230 - 3.360 |
| R-factor | 0.2307 |
| Rwork | 0.230 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.340 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rpim | 0.096 | 0.457 |
| Number of reflections | 89919 | 4072 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.0 M Sodium chloride, 0.1 M Sodium citrate pH 6.0, 13.25% PEG4000 |
