8JIS
Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0003924 | molecular_function | GTPase activity |
A | 0005525 | molecular_function | GTP binding |
A | 0007165 | biological_process | signal transduction |
A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
A | 0019001 | molecular_function | guanyl nucleotide binding |
A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
B | 0001750 | cellular_component | photoreceptor outer segment |
B | 0001917 | cellular_component | photoreceptor inner segment |
B | 0003924 | molecular_function | GTPase activity |
B | 0005515 | molecular_function | protein binding |
B | 0005737 | cellular_component | cytoplasm |
B | 0005834 | cellular_component | heterotrimeric G-protein complex |
B | 0005886 | cellular_component | plasma membrane |
B | 0007165 | biological_process | signal transduction |
B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
B | 0007200 | biological_process | phospholipase C-activating G protein-coupled receptor signaling pathway |
B | 0007204 | biological_process | positive regulation of cytosolic calcium ion concentration |
B | 0008283 | biological_process | cell population proliferation |
B | 0010659 | biological_process | cardiac muscle cell apoptotic process |
B | 0030159 | molecular_function | signaling receptor complex adaptor activity |
B | 0030425 | cellular_component | dendrite |
B | 0030507 | molecular_function | spectrin binding |
B | 0042622 | cellular_component | photoreceptor outer segment membrane |
B | 0044297 | cellular_component | cell body |
B | 0044877 | molecular_function | protein-containing complex binding |
B | 0045202 | cellular_component | synapse |
B | 0047391 | molecular_function | alkylglycerophosphoethanolamine phosphodiesterase activity |
B | 0050909 | biological_process | sensory perception of taste |
B | 0051020 | molecular_function | GTPase binding |
B | 0060041 | biological_process | retina development in camera-type eye |
B | 0071456 | biological_process | cellular response to hypoxia |
G | 0005834 | cellular_component | heterotrimeric G-protein complex |
G | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
G | 0031681 | molecular_function | G-protein beta-subunit binding |
P | 0005179 | molecular_function | hormone activity |
P | 0005576 | cellular_component | extracellular region |
R | 0004888 | molecular_function | transmembrane signaling receptor activity |
R | 0004930 | molecular_function | G protein-coupled receptor activity |
R | 0004967 | molecular_function | glucagon receptor activity |
R | 0005515 | molecular_function | protein binding |
R | 0005886 | cellular_component | plasma membrane |
R | 0007166 | biological_process | cell surface receptor signaling pathway |
R | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
R | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
R | 0007190 | biological_process | activation of adenylate cyclase activity |
R | 0007204 | biological_process | positive regulation of cytosolic calcium ion concentration |
R | 0007611 | biological_process | learning or memory |
R | 0008016 | biological_process | regulation of heart contraction |
R | 0008528 | molecular_function | G protein-coupled peptide receptor activity |
R | 0016020 | cellular_component | membrane |
R | 0017046 | molecular_function | peptide hormone binding |
R | 0019933 | biological_process | cAMP-mediated signaling |
R | 0031204 | biological_process | post-translational protein targeting to membrane, translocation |
R | 0038023 | molecular_function | signaling receptor activity |
R | 0044508 | molecular_function | glucagon-like peptide 1 receptor activity |
R | 0045776 | biological_process | negative regulation of blood pressure |
R | 0045777 | biological_process | positive regulation of blood pressure |
R | 0046879 | biological_process | hormone secretion |
R | 0071377 | biological_process | cellular response to glucagon stimulus |
R | 1990911 | biological_process | response to psychosocial stress |
Functional Information from PROSITE/UniProt
site_id | PS00260 |
Number of Residues | 23 |
Details | GLUCAGON Glucagon / GIP / secretin / VIP family signature. HSQGTFtSDYskyldeqaAKEFI |
Chain | Residue | Details |
P | HIS1-ILE23 |
site_id | PS00649 |
Number of Residues | 25 |
Details | G_PROTEIN_RECEP_F2_1 G-protein coupled receptors family 2 signature 1. CnrtFDeya.CWpdGepgsfvnvsCP |
Chain | Residue | Details |
R | CYS62-PRO86 |
site_id | PS00650 |
Number of Residues | 16 |
Details | G_PROTEIN_RECEP_F2_2 G-protein coupled receptors family 2 signature 2. QGLMVaILYCFvNneV |
Chain | Residue | Details |
R | GLN394-VAL409 |
site_id | PS00678 |
Number of Residues | 15 |
Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS |
Chain | Residue | Details |
B | LEU70-SER84 | |
B | ILE157-ILE171 | |
B | LEU285-ALA299 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:4G5Q |
Chain | Residue | Details |
A | SER151 |
site_id | SWS_FT_FI2 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0007744|PDB:1KJY, ECO:0007744|PDB:1Y3A, ECO:0007744|PDB:2G83, ECO:0007744|PDB:2GTP, ECO:0007744|PDB:2IK8, ECO:0007744|PDB:2OM2, ECO:0007744|PDB:2XNS, ECO:0007744|PDB:3ONW, ECO:0007744|PDB:3QE0, ECO:0007744|PDB:3QI2, ECO:0007744|PDB:3UMR, ECO:0007744|PDB:3UMS, ECO:0007744|PDB:4G5Q |
Chain | Residue | Details |
A | LEU175 | |
R | TYR252-ILE265 | |
R | CYS329-ARG348 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:18434541, ECO:0000269|PubMed:22383884, ECO:0007744|PDB:3QE0 |
Chain | Residue | Details |
A | THR181 |
site_id | SWS_FT_FI4 |
Number of Residues | 1 |
Details | MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250 |
Chain | Residue | Details |
A | ARG178 | |
R | TYR291-TYR305 | |
R | MET371-LYS383 |
site_id | SWS_FT_FI5 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P04896 |
Chain | Residue | Details |
A | GLY40 | |
A | SER47 | |
A | ASP200 | |
A | ASN259 | |
A | SER333 |
site_id | SWS_FT_FI6 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231 |
Chain | Residue | Details |
A | SER319 |
site_id | SWS_FT_FI7 |
Number of Residues | 2 |
Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) |
Chain | Residue | Details |
A | LYS267 |
site_id | SWS_FT_FI8 |
Number of Residues | 21 |
Details | TRANSMEM: Helical; Name=6 => ECO:0000269|PubMed:28514449 |
Chain | Residue | Details |
R | LEU349-VAL370 |
site_id | SWS_FT_FI9 |
Number of Residues | 20 |
Details | TRANSMEM: Helical; Name=7 => ECO:0000269|PubMed:28514449 |
Chain | Residue | Details |
R | LEU384-PHE404 |
site_id | SWS_FT_FI10 |
Number of Residues | 2 |
Details | SITE: Interaction with the endogenous ligand GLP-1 => ECO:0000269|PubMed:19861722 |
Chain | Residue | Details |
R | ARG121 | |
R | GLU128 |
site_id | SWS_FT_FI11 |
Number of Residues | 1 |
Details | MOD_RES: ADP-ribosylcysteine => ECO:0000269|PubMed:21901419 |
Chain | Residue | Details |
R | CYS341 |
site_id | SWS_FT_FI12 |
Number of Residues | 1 |
Details | MOD_RES: ADP-ribosylarginine => ECO:0000269|PubMed:21901419 |
Chain | Residue | Details |
R | ARG348 |
site_id | SWS_FT_FI13 |
Number of Residues | 3 |
Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:22412906 |
Chain | Residue | Details |
R | ASN63 | |
R | ASN82 | |
R | ASN115 |