8JAS

Structure of CRL2APPBP2 bound with RxxGPAA degron (tetramer)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003777	molecular_function	microtubule motor activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005875	cellular_component	microtubule associated complex
A	0006886	biological_process	intracellular protein transport
A	0015031	biological_process	protein transport
A	0016020	cellular_component	membrane
A	0016567	biological_process	protein ubiquitination
A	0030659	cellular_component	cytoplasmic vesicle membrane
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0046907	biological_process	intracellular transport
A	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
B	0003777	molecular_function	microtubule motor activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0005875	cellular_component	microtubule associated complex
B	0006886	biological_process	intracellular protein transport
B	0015031	biological_process	protein transport
B	0016020	cellular_component	membrane
B	0016567	biological_process	protein ubiquitination
B	0030659	cellular_component	cytoplasmic vesicle membrane
B	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0046907	biological_process	intracellular transport
B	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
C	0001222	molecular_function	transcription corepressor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005829	cellular_component	cytosol
C	0006367	biological_process	transcription initiation at RNA polymerase II promoter
C	0006368	biological_process	transcription elongation by RNA polymerase II
C	0016567	biological_process	protein ubiquitination
C	0030891	cellular_component	VCB complex
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0065003	biological_process	protein-containing complex assembly
C	0070449	cellular_component	elongin complex
C	0140958	biological_process	target-directed miRNA degradation
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005730	cellular_component	nucleolus
E	0005829	cellular_component	cytosol
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0030163	biological_process	protein catabolic process
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0030891	cellular_component	VCB complex
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0044877	molecular_function	protein-containing complex binding
E	0097193	biological_process	intrinsic apoptotic signaling pathway
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0160072	molecular_function	ubiquitin ligase complex scaffold activity
G	0001222	molecular_function	transcription corepressor binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005829	cellular_component	cytosol
G	0006367	biological_process	transcription initiation at RNA polymerase II promoter
G	0006368	biological_process	transcription elongation by RNA polymerase II
G	0016567	biological_process	protein ubiquitination
G	0030891	cellular_component	VCB complex
G	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
G	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
G	0065003	biological_process	protein-containing complex assembly
G	0070449	cellular_component	elongin complex
G	0140958	biological_process	target-directed miRNA degradation
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
I	0000082	biological_process	G1/S transition of mitotic cell cycle
I	0004842	molecular_function	ubiquitin-protein transferase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005730	cellular_component	nucleolus
I	0005829	cellular_component	cytosol
I	0006511	biological_process	ubiquitin-dependent protein catabolic process
I	0016567	biological_process	protein ubiquitination
I	0019005	cellular_component	SCF ubiquitin ligase complex
I	0030163	biological_process	protein catabolic process
I	0030674	molecular_function	protein-macromolecule adaptor activity
I	0030891	cellular_component	VCB complex
I	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
I	0031461	cellular_component	cullin-RING ubiquitin ligase complex
I	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
I	0031625	molecular_function	ubiquitin protein ligase binding
I	0044877	molecular_function	protein-containing complex binding
I	0097193	biological_process	intrinsic apoptotic signaling pathway
I	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
I	0160072	molecular_function	ubiquitin ligase complex scaffold activity
J	0003777	molecular_function	microtubule motor activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005874	cellular_component	microtubule
J	0005875	cellular_component	microtubule associated complex
J	0006886	biological_process	intracellular protein transport
J	0015031	biological_process	protein transport
J	0016020	cellular_component	membrane
J	0016567	biological_process	protein ubiquitination
J	0030659	cellular_component	cytoplasmic vesicle membrane
J	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0046907	biological_process	intracellular transport
J	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
K	0003777	molecular_function	microtubule motor activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005856	cellular_component	cytoskeleton
K	0005874	cellular_component	microtubule
K	0005875	cellular_component	microtubule associated complex
K	0006886	biological_process	intracellular protein transport
K	0015031	biological_process	protein transport
K	0016020	cellular_component	membrane
K	0016567	biological_process	protein ubiquitination
K	0030659	cellular_component	cytoplasmic vesicle membrane
K	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0046907	biological_process	intracellular transport
K	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
L	0000082	biological_process	G1/S transition of mitotic cell cycle
L	0004842	molecular_function	ubiquitin-protein transferase activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005730	cellular_component	nucleolus
L	0005829	cellular_component	cytosol
L	0006511	biological_process	ubiquitin-dependent protein catabolic process
L	0016567	biological_process	protein ubiquitination
L	0019005	cellular_component	SCF ubiquitin ligase complex
L	0030163	biological_process	protein catabolic process
L	0030674	molecular_function	protein-macromolecule adaptor activity
L	0030891	cellular_component	VCB complex
L	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
L	0031461	cellular_component	cullin-RING ubiquitin ligase complex
L	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
L	0031625	molecular_function	ubiquitin protein ligase binding
L	0044877	molecular_function	protein-containing complex binding
L	0097193	biological_process	intrinsic apoptotic signaling pathway
L	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
L	0160072	molecular_function	ubiquitin ligase complex scaffold activity
M	0001222	molecular_function	transcription corepressor binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005829	cellular_component	cytosol
M	0006367	biological_process	transcription initiation at RNA polymerase II promoter
M	0006368	biological_process	transcription elongation by RNA polymerase II
M	0016567	biological_process	protein ubiquitination
M	0030891	cellular_component	VCB complex
M	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
M	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
M	0031625	molecular_function	ubiquitin protein ligase binding
M	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
M	0065003	biological_process	protein-containing complex assembly
M	0070449	cellular_component	elongin complex
M	0140958	biological_process	target-directed miRNA degradation
N	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0001222	molecular_function	transcription corepressor binding
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005829	cellular_component	cytosol
Q	0006367	biological_process	transcription initiation at RNA polymerase II promoter
Q	0006368	biological_process	transcription elongation by RNA polymerase II
Q	0016567	biological_process	protein ubiquitination
Q	0030891	cellular_component	VCB complex
Q	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
Q	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
Q	0031625	molecular_function	ubiquitin protein ligase binding
Q	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Q	0065003	biological_process	protein-containing complex assembly
Q	0070449	cellular_component	elongin complex
Q	0140958	biological_process	target-directed miRNA degradation
R	0000165	biological_process	MAPK cascade
R	0000209	biological_process	protein polyubiquitination
R	0004842	molecular_function	ubiquitin-protein transferase activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006281	biological_process	DNA repair
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0006513	biological_process	protein monoubiquitination
R	0006974	biological_process	DNA damage response
R	0007283	biological_process	spermatogenesis
R	0008270	molecular_function	zinc ion binding
R	0016567	biological_process	protein ubiquitination
R	0016740	molecular_function	transferase activity
R	0019005	cellular_component	SCF ubiquitin ligase complex
R	0019788	molecular_function	NEDD8 transferase activity
R	0030163	biological_process	protein catabolic process
R	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R	0031461	cellular_component	cullin-RING ubiquitin ligase complex
R	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
R	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
R	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
R	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
R	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
R	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
R	0031625	molecular_function	ubiquitin protein ligase binding
R	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
R	0032480	biological_process	negative regulation of type I interferon production
R	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
R	0034644	biological_process	cellular response to UV
R	0042110	biological_process	T cell activation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0043687	biological_process	post-translational protein modification
R	0045116	biological_process	protein neddylation
R	0045732	biological_process	positive regulation of protein catabolic process
R	0046872	molecular_function	metal ion binding
R	0060090	molecular_function	molecular adaptor activity
R	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
R	0061630	molecular_function	ubiquitin protein ligase activity
R	0061663	molecular_function	NEDD8 ligase activity
R	0062197	biological_process	cellular response to chemical stress
R	0070936	biological_process	protein K48-linked ubiquitination
R	0071230	biological_process	cellular response to amino acid stimulus
R	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
R	0097602	molecular_function	cullin family protein binding
R	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
R	1900076	biological_process	regulation of cellular response to insulin stimulus
R	1902499	biological_process	positive regulation of protein autoubiquitination
R	1902883	biological_process	negative regulation of response to oxidative stress
R	1904263	biological_process	positive regulation of TORC1 signaling
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0000082	biological_process	G1/S transition of mitotic cell cycle
U	0004842	molecular_function	ubiquitin-protein transferase activity
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005730	cellular_component	nucleolus
U	0005829	cellular_component	cytosol
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0016567	biological_process	protein ubiquitination
U	0019005	cellular_component	SCF ubiquitin ligase complex
U	0030163	biological_process	protein catabolic process
U	0030674	molecular_function	protein-macromolecule adaptor activity
U	0030891	cellular_component	VCB complex
U	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
U	0031461	cellular_component	cullin-RING ubiquitin ligase complex
U	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
U	0031625	molecular_function	ubiquitin protein ligase binding
U	0044877	molecular_function	protein-containing complex binding
U	0097193	biological_process	intrinsic apoptotic signaling pathway
U	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
U	0160072	molecular_function	ubiquitin ligase complex scaffold activity
V	0000165	biological_process	MAPK cascade
V	0000209	biological_process	protein polyubiquitination
V	0004842	molecular_function	ubiquitin-protein transferase activity
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005654	cellular_component	nucleoplasm
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0006281	biological_process	DNA repair
V	0006511	biological_process	ubiquitin-dependent protein catabolic process
V	0006513	biological_process	protein monoubiquitination
V	0006974	biological_process	DNA damage response
V	0007283	biological_process	spermatogenesis
V	0008270	molecular_function	zinc ion binding
V	0016567	biological_process	protein ubiquitination
V	0016740	molecular_function	transferase activity
V	0019005	cellular_component	SCF ubiquitin ligase complex
V	0019788	molecular_function	NEDD8 transferase activity
V	0030163	biological_process	protein catabolic process
V	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
V	0031461	cellular_component	cullin-RING ubiquitin ligase complex
V	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
V	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
V	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
V	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
V	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
V	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
V	0031625	molecular_function	ubiquitin protein ligase binding
V	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
V	0032480	biological_process	negative regulation of type I interferon production
V	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
V	0034644	biological_process	cellular response to UV
V	0042110	biological_process	T cell activation
V	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0043687	biological_process	post-translational protein modification
V	0045116	biological_process	protein neddylation
V	0045732	biological_process	positive regulation of protein catabolic process
V	0046872	molecular_function	metal ion binding
V	0060090	molecular_function	molecular adaptor activity
V	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
V	0061630	molecular_function	ubiquitin protein ligase activity
V	0061663	molecular_function	NEDD8 ligase activity
V	0062197	biological_process	cellular response to chemical stress
V	0070936	biological_process	protein K48-linked ubiquitination
V	0071230	biological_process	cellular response to amino acid stimulus
V	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
V	0097602	molecular_function	cullin family protein binding
V	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
V	1900076	biological_process	regulation of cellular response to insulin stimulus
V	1902499	biological_process	positive regulation of protein autoubiquitination
V	1902883	biological_process	negative regulation of response to oxidative stress
V	1904263	biological_process	positive regulation of TORC1 signaling

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
E	ILE718-ALA745

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	90
Details	ZN_FING: RING-type => ECO:0000255|PROSITE-ProRule:PRU00175

Chain	Residue	Details
R	CYS53-ASN98
V	CYS53-ASN98
M	MET1
Q	MET1

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DPL, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O

Chain	Residue	Details
R	CYS42
R	CYS94
R	ASP97
V	CYS42
V	CYS45
V	CYS53
V	CYS56
V	CYS68
V	CYS75
V	HIS77
V	HIS80
R	CYS45
V	HIS82
V	CYS94
V	ASP97
R	CYS53
R	CYS56
R	CYS68
R	CYS75
R	HIS77
R	HIS80
R	HIS82

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O

Chain	Residue	Details
R	CYS83
V	CYS83
G	SER108
G	SER111
M	SER108
M	SER111
Q	SER108
Q	SER111

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
R	MET1
V	MET1

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed => ECO:0000269|Ref.8, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
R	ALA2
V	ALA2

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
R	THR9
V	THR9

---