8I3V
Cryo-EM structure of human norepinephrine transporter NET in the presence of the antidepressant escitalopram in an inward-open state at resolution of 2.85 angstrom.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003779 | molecular_function | actin binding |
| A | 0005326 | molecular_function | neurotransmitter transmembrane transporter activity |
| A | 0005330 | molecular_function | dopamine:sodium symporter activity |
| A | 0005334 | molecular_function | norepinephrine:sodium symporter activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006836 | biological_process | neurotransmitter transport |
| A | 0006865 | biological_process | amino acid transport |
| A | 0007268 | biological_process | chemical synaptic transmission |
| A | 0008504 | molecular_function | monoamine transmembrane transporter activity |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009986 | cellular_component | cell surface |
| A | 0015293 | molecular_function | symporter activity |
| A | 0015844 | biological_process | monoamine transport |
| A | 0015874 | biological_process | norepinephrine transport |
| A | 0016020 | cellular_component | membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0030424 | cellular_component | axon |
| A | 0030672 | cellular_component | synaptic vesicle membrane |
| A | 0032809 | cellular_component | neuronal cell body membrane |
| A | 0035725 | biological_process | sodium ion transmembrane transport |
| A | 0042734 | cellular_component | presynaptic membrane |
| A | 0043014 | molecular_function | alpha-tubulin binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048265 | biological_process | response to pain |
| A | 0048487 | molecular_function | beta-tubulin binding |
| A | 0051583 | biological_process | dopamine uptake involved in synaptic transmission |
| A | 0051620 | biological_process | norepinephrine uptake |
| A | 0070050 | biological_process | neuron cellular homeostasis |
| A | 0098810 | biological_process | neurotransmitter reuptake |
Functional Information from PROSITE/UniProt
| site_id | PS00610 |
| Number of Residues | 15 |
| Details | NA_NEUROTRAN_SYMP_1 Sodium:neurotransmitter symporter family signature 1. WRFPYlcykNGGGaF |
| Chain | Residue | Details |
| A | TRP80-PHE94 |
| site_id | PS00754 |
| Number of Residues | 21 |
| Details | NA_NEUROTRAN_SYMP_2 Sodium:neurotransmitter symporter family signature 2. YLfsSFTlnLPWtdCghtwNS |
| Chain | Residue | Details |
| A | TYR162-SER182 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 176 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | MET1-LYS62 | |
| A | GLY117-LYS135 | |
| A | GLY255-LYS257 | |
| A | ASN333-ASN338 | |
| A | THR429-LYS443 | |
| A | SER493-LYS522 | |
| A | PRO570-ILE617 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=1 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | ILE63-LYS88 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 134 |
| Details | TOPO_DOM: Extracellular => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | ASN89-GLY92 | |
| A | PHE167-PRO233 | |
| A | THR283-THR306 | |
| A | SER363-THR402 | |
| A | GLY465 | |
| A | ASP546-TYR548 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | ALA93-LEU116 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 30 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | GLY136-SER166 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | GLN234-LYS254 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 24 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | THR258-VAL282 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | VAL307-TYR332 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | CYS339-PHE362 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=8 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | PHE403-ILE428 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=9 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | LEU444-GLY464 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 26 |
| Details | TRANSMEM: Helical; Name=10 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | ILE466-VAL492 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=11 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | PHE523-TYR545 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=12 => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | ILE549-VAL569 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 9 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:Q7K4Y6 |
| Chain | Residue | Details |
| A | GLY71 | |
| A | ALA73 | |
| A | VAL74 | |
| A | ASN78 | |
| A | SER318 | |
| A | ASN350 | |
| A | LEU415 | |
| A | ASP418 | |
| A | SER419 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN184 | |
| A | ASN192 | |
| A | ASN198 |
