8HRP

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NAD and G3P

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
A	0005737	cellular_component	cytoplasm
A	0006006	biological_process	glucose metabolic process
A	0006096	biological_process	glycolytic process
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
B	0000166	molecular_function	nucleotide binding
B	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
B	0005737	cellular_component	cytoplasm
B	0006006	biological_process	glucose metabolic process
B	0006096	biological_process	glycolytic process
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
C	0000166	molecular_function	nucleotide binding
C	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
C	0005737	cellular_component	cytoplasm
C	0006006	biological_process	glucose metabolic process
C	0006096	biological_process	glycolytic process
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
D	0000166	molecular_function	nucleotide binding
D	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
D	0005737	cellular_component	cytoplasm
D	0006006	biological_process	glucose metabolic process
D	0006096	biological_process	glycolytic process
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
E	0000166	molecular_function	nucleotide binding
E	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
E	0005737	cellular_component	cytoplasm
E	0006006	biological_process	glucose metabolic process
E	0006096	biological_process	glycolytic process
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0050661	molecular_function	NADP binding
E	0051287	molecular_function	NAD binding
F	0000166	molecular_function	nucleotide binding
F	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
F	0005737	cellular_component	cytoplasm
F	0006006	biological_process	glucose metabolic process
F	0006096	biological_process	glycolytic process
F	0016491	molecular_function	oxidoreductase activity
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0050661	molecular_function	NADP binding
F	0051287	molecular_function	NAD binding
G	0000166	molecular_function	nucleotide binding
G	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
G	0005737	cellular_component	cytoplasm
G	0006006	biological_process	glucose metabolic process
G	0006096	biological_process	glycolytic process
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0050661	molecular_function	NADP binding
G	0051287	molecular_function	NAD binding
H	0000166	molecular_function	nucleotide binding
H	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
H	0005737	cellular_component	cytoplasm
H	0006006	biological_process	glucose metabolic process
H	0006096	biological_process	glycolytic process
H	0016491	molecular_function	oxidoreductase activity
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0050661	molecular_function	NADP binding
H	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
A	ALA151-LEU158

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P00362

Chain	Residue	Details
A	CYS153
B	CYS153
C	CYS153
D	CYS153
E	CYS153
F	CYS153
G	CYS153
H	CYS153

---

site_id	SWS_FT_FI2
Number of Residues	80
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P00362

Chain	Residue	Details
A	ARG12
A	ASN315
B	ARG12
B	ASP35
B	ARG79
B	SER121
B	SER152
B	THR183
B	ARG198
B	THR211
B	ARG234
A	ASP35
B	ASN315
C	ARG12
C	ASP35
C	ARG79
C	SER121
C	SER152
C	THR183
C	ARG198
C	THR211
C	ARG234
A	ARG79
C	ASN315
D	ARG12
D	ASP35
D	ARG79
D	SER121
D	SER152
D	THR183
D	ARG198
D	THR211
D	ARG234
A	SER121
D	ASN315
E	ARG12
E	ASP35
E	ARG79
E	SER121
E	SER152
E	THR183
E	ARG198
E	THR211
E	ARG234
A	SER152
E	ASN315
F	ARG12
F	ASP35
F	ARG79
F	SER121
F	SER152
F	THR183
F	ARG198
F	THR211
F	ARG234
A	THR183
F	ASN315
G	ARG12
G	ASP35
G	ARG79
G	SER121
G	SER152
G	THR183
G	ARG198
G	THR211
G	ARG234
A	ARG198
G	ASN315
H	ARG12
H	ASP35
H	ARG79
H	SER121
H	SER152
H	THR183
H	ARG198
H	THR211
H	ARG234
A	THR211
H	ASN315
A	ARG234

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Activates thiol group during catalysis => ECO:0000250|UniProtKB:P00362

Chain	Residue	Details
A	HIS180
B	HIS180
C	HIS180
D	HIS180
E	HIS180
F	HIS180
G	HIS180
H	HIS180

---