8HQC

Structure of a GPCR-G protein in complex with a natural peptide agonist

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001774	biological_process	microglial cell activation
A	0002430	biological_process	complement receptor mediated signaling pathway
A	0002684	biological_process	positive regulation of immune system process
A	0004875	molecular_function	complement receptor activity
A	0004878	molecular_function	complement component C5a receptor activity
A	0004930	molecular_function	G protein-coupled receptor activity
A	0005886	cellular_component	plasma membrane
A	0006935	biological_process	chemotaxis
A	0006954	biological_process	inflammatory response
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
A	0007204	biological_process	positive regulation of cytosolic calcium ion concentration
A	0010575	biological_process	positive regulation of vascular endothelial growth factor production
A	0010759	biological_process	positive regulation of macrophage chemotaxis
A	0016020	cellular_component	membrane
A	0016323	cellular_component	basolateral plasma membrane
A	0030593	biological_process	neutrophil chemotaxis
A	0031410	cellular_component	cytoplasmic vesicle
A	0032494	biological_process	response to peptidoglycan
A	0038178	biological_process	complement component C5a signaling pathway
A	0042789	biological_process	mRNA transcription by RNA polymerase II
A	0045177	cellular_component	apical part of cell
A	0045766	biological_process	positive regulation of angiogenesis
A	0048143	biological_process	astrocyte activation
A	0050679	biological_process	positive regulation of epithelial cell proliferation
A	0050830	biological_process	defense response to Gram-positive bacterium
A	0050890	biological_process	cognition
A	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
A	0090023	biological_process	positive regulation of neutrophil chemotaxis
A	0097242	biological_process	amyloid-beta clearance
A	0099172	biological_process	presynapse organization
B	0003924	molecular_function	GTPase activity
B	0005525	molecular_function	GTP binding
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
B	0019001	molecular_function	guanyl nucleotide binding
B	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0001750	cellular_component	photoreceptor outer segment
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005829	cellular_component	cytosol
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
C	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
C	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
C	0007265	biological_process	Ras protein signal transduction
C	0008283	biological_process	cell population proliferation
C	0016020	cellular_component	membrane
C	0030159	molecular_function	signaling receptor complex adaptor activity
C	0044877	molecular_function	protein-containing complex binding
C	0045202	cellular_component	synapse
C	0050909	biological_process	sensory perception of taste
C	0051020	molecular_function	GTPase binding
C	0060041	biological_process	retina development in camera-type eye
C	0070062	cellular_component	extracellular exosome
C	0071380	biological_process	cellular response to prostaglandin E stimulus
C	0071870	biological_process	cellular response to catecholamine stimulus
C	0097381	cellular_component	photoreceptor disc membrane
C	1903561	cellular_component	extracellular vesicle
D	0005576	cellular_component	extracellular region
D	0006954	biological_process	inflammatory response
D	0006956	biological_process	complement activation
G	0005515	molecular_function	protein binding
G	0005834	cellular_component	heterotrimeric G-protein complex
G	0005886	cellular_component	plasma membrane
G	0007165	biological_process	signal transduction
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
G	0016020	cellular_component	membrane
G	0031681	molecular_function	G-protein beta-subunit binding
G	0045202	cellular_component	synapse
G	0048144	biological_process	fibroblast proliferation
G	0070062	cellular_component	extracellular exosome
G	0071380	biological_process	cellular response to prostaglandin E stimulus
G	0071870	biological_process	cellular response to catecholamine stimulus

Functional Information from PROSITE/UniProt

site_id	PS00237
Number of Residues	17
Details	G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASIlLLATISADRFLlV

Chain	Residue	Details
A	ALA122-VAL138

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
C	LEU70-SER84
C	ILE157-ILE171
C	LEU285-ALA299

---

site_id	PS01177
Number of Residues	35
Details	ANAPHYLATOXIN_1 Anaphylatoxin domain signature. CCydGacvnnde.TCEqraarisl.GprCikafte.CC

Chain	Residue	Details
D	CYS21-CYS55

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	39
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	16
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P21730","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	13
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	9
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	7
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	5
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P18872","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	1
Details	Modified residue: {"description":"5-glutamyl histamine","evidences":[{"source":"UniProtKB","id":"P18872","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	Modified residue: {"description":"ADP-ribosylcysteine; by pertussis toxin","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"33408414","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	48
Details	Repeat: {"description":"WD 1","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	45
Details	Repeat: {"description":"WD 2","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	40
Details	Repeat: {"description":"WD 3","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	41
Details	Repeat: {"description":"WD 4","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	43
Details	Repeat: {"description":"WD 5","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	41
Details	Repeat: {"description":"WD 6","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	30
Details	Repeat: {"description":"WD 7","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	1
Details	Modified residue: {"description":"Phosphohistidine","evidences":[{"source":"UniProtKB","id":"P62871","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	34
Details	Domain: {"description":"Anaphylatoxin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00022","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	29
Details	Region: {"description":"Involved in C5AR1 binding","evidences":[{"source":"PubMed","id":"9553099","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"16335952","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18536718","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3CU7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3KLS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3KM9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---