8HFF
Cryo-EM structure of human norepinephrine transporter NET in the presence of norepinephrine in an inward-open state at resolution of 2.9 angstrom.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003779 | molecular_function | actin binding |
| A | 0005326 | molecular_function | neurotransmitter transmembrane transporter activity |
| A | 0005328 | molecular_function | neurotransmitter:sodium symporter activity |
| A | 0005330 | molecular_function | dopamine:sodium symporter activity |
| A | 0005334 | molecular_function | norepinephrine:sodium symporter activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006836 | biological_process | neurotransmitter transport |
| A | 0006865 | biological_process | amino acid transport |
| A | 0007268 | biological_process | chemical synaptic transmission |
| A | 0008504 | molecular_function | monoamine transmembrane transporter activity |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009986 | cellular_component | cell surface |
| A | 0015293 | molecular_function | symporter activity |
| A | 0015378 | molecular_function | sodium:chloride symporter activity |
| A | 0015844 | biological_process | monoamine transport |
| A | 0015874 | biological_process | norepinephrine transport |
| A | 0016020 | cellular_component | membrane |
| A | 0030424 | cellular_component | axon |
| A | 0030672 | cellular_component | synaptic vesicle membrane |
| A | 0032809 | cellular_component | neuronal cell body membrane |
| A | 0035725 | biological_process | sodium ion transmembrane transport |
| A | 0042734 | cellular_component | presynaptic membrane |
| A | 0042995 | cellular_component | cell projection |
| A | 0043005 | cellular_component | neuron projection |
| A | 0043014 | molecular_function | alpha-tubulin binding |
| A | 0045202 | cellular_component | synapse |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048265 | biological_process | response to pain |
| A | 0048487 | molecular_function | beta-tubulin binding |
| A | 0051583 | biological_process | dopamine uptake involved in synaptic transmission |
| A | 0051620 | biological_process | norepinephrine uptake |
| A | 0070050 | biological_process | neuron cellular homeostasis |
| A | 0090493 | biological_process | catecholamine uptake |
| A | 0098810 | biological_process | neurotransmitter reuptake |
| A | 1902476 | biological_process | chloride transmembrane transport |
Functional Information from PROSITE/UniProt
| site_id | PS00610 |
| Number of Residues | 15 |
| Details | NA_NEUROTRAN_SYMP_1 Sodium:neurotransmitter symporter family signature 1. WRFPYlcykNGGGaF |
| Chain | Residue | Details |
| A | TRP80-PHE94 |
| site_id | PS00754 |
| Number of Residues | 21 |
| Details | NA_NEUROTRAN_SYMP_2 Sodium:neurotransmitter symporter family signature 2. YLfsSFTlnLPWtdCghtwNS |
| Chain | Residue | Details |
| A | TYR162-SER182 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 68 |
| Details | Topological domain: {"description":"Extracellular","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 23 |
| Details | Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 115 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 30 |
| Details | Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 20 |
| Details | Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 24 |
| Details | Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 23 |
| Details | Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 25 |
| Details | Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 20 |
| Details | Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 26 |
| Details | Transmembrane: {"description":"Helical; Name=10","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 22 |
| Details | Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 20 |
| Details | Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"UniProtKB","id":"Q7K4Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"38750358","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8YR2","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"38750358","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Y95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YR2","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"39048818","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8HFF","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"38750358","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Y95","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
