8GX3
The crystal structure of human Calpain-1 protease core in complex with 14c
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001533 | cellular_component | cornified envelope |
| A | 0004198 | molecular_function | calcium-dependent cysteine-type endopeptidase activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005764 | cellular_component | lysosome |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0006508 | biological_process | proteolysis |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008234 | molecular_function | cysteine-type peptidase activity |
| A | 0008284 | biological_process | positive regulation of cell population proliferation |
| A | 0016020 | cellular_component | membrane |
| A | 0016241 | biological_process | regulation of macroautophagy |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0032801 | biological_process | receptor catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050790 | biological_process | regulation of catalytic activity |
| A | 0060056 | biological_process | mammary gland involution |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0097264 | biological_process | self proteolysis |
| A | 0098989 | biological_process | NMDA selective glutamate receptor signaling pathway |
| A | 0110158 | cellular_component | calpain complex |
| A | 1904813 | cellular_component | ficolin-1-rich granule lumen |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DRDGNGKLGlvEF |
| Chain | Residue | Details |
| A | ASP598-PHE610 | |
| A | ASP628-MET640 |
| site_id | PS00139 |
| Number of Residues | 12 |
| Details | THIOL_PROTEASE_CYS Eukaryotic thiol (cysteine) proteases cysteine active site. QGaLGDCWLlAA |
| Chain | Residue | Details |
| A | GLN109-ALA120 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | ACT_SITE: ACT_SITE => ECO:0000250 |
| Chain | Residue | Details |
| A | CYS115 | |
| A | HIS272 | |
| A | ASN296 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000305 |
| Chain | Residue | Details |
| A | GLN109 | |
| A | ASP114 | |
| A | ASN316 | |
| A | ASP318 | |
| A | ASP323 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448 |
| Chain | Residue | Details |
| A | ASP598 | |
| A | GLU639 | |
| A | ASP600 | |
| A | ASN602 | |
| A | LYS604 | |
| A | GLU609 | |
| A | ASP628 | |
| A | ASP630 | |
| A | SER632 | |
| A | SER634 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | SITE: Cleavage; for 78 kDa form |
| Chain | Residue | Details |
| A | SER15 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | SITE: Cleavage; for 75 kDa form |
| Chain | Residue | Details |
| A | GLY27 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylserine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| A | SER2 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | THR354 |
