8GV3

The cryo-EM structure of GSNOR with NYY001

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001523	biological_process	retinoid metabolic process
A	0003016	biological_process	respiratory system process
A	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
A	0004024	molecular_function	alcohol dehydrogenase (NAD+) activity, zinc-dependent
A	0005504	molecular_function	fatty acid binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0008270	molecular_function	zinc ion binding
A	0009055	molecular_function	electron transfer activity
A	0010430	biological_process	fatty acid omega-oxidation
A	0016491	molecular_function	oxidoreductase activity
A	0018467	molecular_function	formaldehyde dehydrogenase (NAD+) activity
A	0032496	biological_process	response to lipopolysaccharide
A	0042802	molecular_function	identical protein binding
A	0044281	biological_process	small molecule metabolic process
A	0045777	biological_process	positive regulation of blood pressure
A	0046294	biological_process	formaldehyde catabolic process
A	0046872	molecular_function	metal ion binding
A	0051409	biological_process	response to nitrosative stress
A	0051775	biological_process	response to redox state
A	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
A	0070062	cellular_component	extracellular exosome
A	0106321	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity
A	0106322	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity
B	0001523	biological_process	retinoid metabolic process
B	0003016	biological_process	respiratory system process
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0004024	molecular_function	alcohol dehydrogenase (NAD+) activity, zinc-dependent
B	0005504	molecular_function	fatty acid binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0008270	molecular_function	zinc ion binding
B	0009055	molecular_function	electron transfer activity
B	0010430	biological_process	fatty acid omega-oxidation
B	0016491	molecular_function	oxidoreductase activity
B	0018467	molecular_function	formaldehyde dehydrogenase (NAD+) activity
B	0032496	biological_process	response to lipopolysaccharide
B	0042802	molecular_function	identical protein binding
B	0044281	biological_process	small molecule metabolic process
B	0045777	biological_process	positive regulation of blood pressure
B	0046294	biological_process	formaldehyde catabolic process
B	0046872	molecular_function	metal ion binding
B	0051409	biological_process	response to nitrosative stress
B	0051775	biological_process	response to redox state
B	0051903	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
B	0070062	cellular_component	extracellular exosome
B	0106321	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity
B	0106322	molecular_function	S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity

Functional Information from PROSITE/UniProt

site_id	PS00059
Number of Residues	15
Details	ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEgAGIvesvGegV

Chain	Residue	Details
A	GLY65-VAL79

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	BINDING: BINDING => ECO:0000269|PubMed:3365377

Chain	Residue	Details
A	CYS44
B	CYS96
B	CYS99
B	CYS102
B	CYS110
B	CYS173
A	HIS66
A	CYS96
A	CYS99
A	CYS102
A	CYS110
A	CYS173
B	CYS44
B	HIS66

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site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Important for FDH activity and activation by fatty acids => ECO:0000269|PubMed:8460164, ECO:0000269|PubMed:8494891

Chain	Residue	Details
A	ARG114
B	ARG114

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site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:19413330

Chain	Residue	Details
A	ALA1
B	ALA1

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site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P28474

Chain	Residue	Details
A	LYS232
A	LYS314
B	LYS232
B	LYS314

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site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER246
B	SER246

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site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER323
A	SER350
B	SER323
B	SER350

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	12
Details	M-CSA 464

Chain	Residue	Details
A	CYS44	metal ligand
A	ARG114	activator
A	CYS173	metal ligand
A	ARG368	steric role
A	HIS45	proton shuttle (general acid/base)
A	THR46	proton shuttle (general acid/base)
A	HIS66	metal ligand
A	GLU67	metal ligand
A	CYS96	metal ligand
A	CYS99	metal ligand
A	CYS102	metal ligand
A	CYS110	metal ligand

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site_id	MCSA2
Number of Residues	12
Details	M-CSA 464

Chain	Residue	Details
B	CYS44	metal ligand
B	ARG114	activator
B	CYS173	metal ligand
B	ARG368	steric role
B	HIS45	proton shuttle (general acid/base)
B	THR46	proton shuttle (general acid/base)
B	HIS66	metal ligand
B	GLU67	metal ligand
B	CYS96	metal ligand
B	CYS99	metal ligand
B	CYS102	metal ligand
B	CYS110	metal ligand

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