Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8GRB

Crystal structure of a constitutively active mutant of the alpha beta heterodimer of human IDH3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005975	biological_process	carbohydrate metabolic process
A	0006099	biological_process	tricarboxylic acid cycle
A	0006102	biological_process	isocitrate metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
B	0000287	molecular_function	magnesium ion binding
B	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005975	biological_process	carbohydrate metabolic process
B	0006099	biological_process	tricarboxylic acid cycle
B	0006102	biological_process	isocitrate metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
C	0000287	molecular_function	magnesium ion binding
C	0006099	biological_process	tricarboxylic acid cycle
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0051287	molecular_function	NAD binding
D	0000287	molecular_function	magnesium ion binding
D	0006099	biological_process	tricarboxylic acid cycle
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0051287	molecular_function	NAD binding
E	0000287	molecular_function	magnesium ion binding
E	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0005975	biological_process	carbohydrate metabolic process
E	0006099	biological_process	tricarboxylic acid cycle
E	0006102	biological_process	isocitrate metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
E	0046872	molecular_function	metal ion binding
E	0051287	molecular_function	NAD binding
F	0000287	molecular_function	magnesium ion binding
F	0006099	biological_process	tricarboxylic acid cycle
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0051287	molecular_function	NAD binding
G	0000287	molecular_function	magnesium ion binding
G	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005739	cellular_component	mitochondrion
G	0005759	cellular_component	mitochondrial matrix
G	0005975	biological_process	carbohydrate metabolic process
G	0006099	biological_process	tricarboxylic acid cycle
G	0006102	biological_process	isocitrate metabolic process
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
G	0046872	molecular_function	metal ion binding
G	0051287	molecular_function	NAD binding
H	0000287	molecular_function	magnesium ion binding
H	0006099	biological_process	tricarboxylic acid cycle
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0051287	molecular_function	NAD binding
I	0000287	molecular_function	magnesium ion binding
I	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005739	cellular_component	mitochondrion
I	0005759	cellular_component	mitochondrial matrix
I	0005975	biological_process	carbohydrate metabolic process
I	0006099	biological_process	tricarboxylic acid cycle
I	0006102	biological_process	isocitrate metabolic process
I	0016491	molecular_function	oxidoreductase activity
I	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
I	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
I	0046872	molecular_function	metal ion binding
I	0051287	molecular_function	NAD binding
J	0000287	molecular_function	magnesium ion binding
J	0006099	biological_process	tricarboxylic acid cycle
J	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
J	0051287	molecular_function	NAD binding
K	0000287	molecular_function	magnesium ion binding
K	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005739	cellular_component	mitochondrion
K	0005759	cellular_component	mitochondrial matrix
K	0005975	biological_process	carbohydrate metabolic process
K	0006099	biological_process	tricarboxylic acid cycle
K	0006102	biological_process	isocitrate metabolic process
K	0016491	molecular_function	oxidoreductase activity
K	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
K	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
K	0046872	molecular_function	metal ion binding
K	0051287	molecular_function	NAD binding
L	0000287	molecular_function	magnesium ion binding
L	0006099	biological_process	tricarboxylic acid cycle
L	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
L	0051287	molecular_function	NAD binding
M	0000287	molecular_function	magnesium ion binding
M	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005739	cellular_component	mitochondrion
M	0005759	cellular_component	mitochondrial matrix
M	0005975	biological_process	carbohydrate metabolic process
M	0006099	biological_process	tricarboxylic acid cycle
M	0006102	biological_process	isocitrate metabolic process
M	0016491	molecular_function	oxidoreductase activity
M	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
M	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
M	0046872	molecular_function	metal ion binding
M	0051287	molecular_function	NAD binding
N	0000287	molecular_function	magnesium ion binding
N	0006099	biological_process	tricarboxylic acid cycle
N	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
N	0051287	molecular_function	NAD binding
O	0000287	molecular_function	magnesium ion binding
O	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005739	cellular_component	mitochondrion
O	0005759	cellular_component	mitochondrial matrix
O	0005975	biological_process	carbohydrate metabolic process
O	0006099	biological_process	tricarboxylic acid cycle
O	0006102	biological_process	isocitrate metabolic process
O	0016491	molecular_function	oxidoreductase activity
O	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
O	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
O	0046872	molecular_function	metal ion binding
O	0051287	molecular_function	NAD binding
P	0000287	molecular_function	magnesium ion binding
P	0006099	biological_process	tricarboxylic acid cycle
P	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
P	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	20
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NLYGNIiDNlaAglv.GGAGV

Chain	Residue	Details
C	ASN237-VAL256
B	ASN226-VAL245

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
C	LYS165
A	ASP206
A	ASP230
A	ASP234
E	ARG88
E	ARG98
E	ARG119
E	ASP206
E	ASP230
E	ASP234
G	ARG88
D	LYS165
G	ARG98
G	ARG119
G	ASP206
G	ASP230
G	ASP234
I	ARG88
I	ARG98
I	ARG119
I	ASP206
I	ASP230
F	LYS165
I	ASP234
K	ARG88
K	ARG98
K	ARG119
K	ASP206
K	ASP230
K	ASP234
M	ARG88
M	ARG98
M	ARG119
H	LYS165
M	ASP206
M	ASP230
M	ASP234
O	ARG88
O	ARG98
O	ARG119
O	ASP206
O	ASP230
O	ASP234
J	LYS165
L	LYS165
N	LYS165
P	LYS165
A	ARG119

site_id	SWS_FT_FI2
Number of Residues	8
Details	SITE: Critical for catalysis => ECO:0000269\|PubMed:28098230, ECO:0000269\|PubMed:28139779

Chain	Residue	Details
B	TYR126
A	TYR126
E	TYR126
G	TYR126
I	TYR126
K	TYR126
M	TYR126
O	TYR126

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Critical for catalysis => ECO:0000269\|PubMed:28098230

Chain	Residue	Details
B	LYS173
A	LYS173
E	LYS173
G	LYS173
I	LYS173
K	LYS173
M	LYS173
O	LYS173

site_id	SWS_FT_FI4
Number of Residues	16
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:Q9D6R2

Chain	Residue	Details
B	LYS50
I	LYS323
K	LYS50
K	LYS323
M	LYS50
M	LYS323
O	LYS50
O	LYS323
B	LYS323
A	LYS50
A	LYS323
E	LYS50
E	LYS323
G	LYS50
G	LYS323
I	LYS50

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:Q9D6R2

Chain	Residue	Details
B	THR74
A	THR74
E	THR74
G	THR74
I	THR74
K	THR74
M	THR74
O	THR74

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q9D6R2

Chain	Residue	Details
B	LYS196
A	LYS196
E	LYS196
G	LYS196
I	LYS196
K	LYS196
M	LYS196
O	LYS196

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:Q9D6R2

Chain	Residue	Details
B	LYS316
A	LYS316
E	LYS316
G	LYS316
I	LYS316
K	LYS316
M	LYS316
O	LYS316

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)