8GHD

The structure of h12-LOX in hexameric form bound to inhibitor ML355 and arachidonic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001676	biological_process	long-chain fatty acid metabolic process
A	0004052	molecular_function	arachidonate 12(S)-lipoxygenase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006690	biological_process	icosanoid metabolic process
A	0010656	biological_process	negative regulation of muscle cell apoptotic process
A	0016020	cellular_component	membrane
A	0016165	molecular_function	linoleate 13S-lipoxygenase activity
A	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
A	0016787	molecular_function	hydrolase activity
A	0019369	biological_process	arachidonate metabolic process
A	0019372	biological_process	lipoxygenase pathway
A	0019395	biological_process	fatty acid oxidation
A	0033559	biological_process	unsaturated fatty acid metabolic process
A	0034440	biological_process	lipid oxidation
A	0042383	cellular_component	sarcolemma
A	0042554	biological_process	superoxide anion generation
A	0043651	biological_process	linoleic acid metabolic process
A	0046872	molecular_function	metal ion binding
A	0047977	molecular_function	hepoxilin-epoxide hydrolase activity
A	0050473	molecular_function	arachidonate 15-lipoxygenase activity
A	0051122	biological_process	hepoxilin biosynthetic process
A	0051213	molecular_function	dioxygenase activity
A	0061436	biological_process	establishment of skin barrier
A	0070062	cellular_component	extracellular exosome
A	0090331	biological_process	negative regulation of platelet aggregation
A	1901751	biological_process	leukotriene A4 metabolic process
A	2001303	biological_process	lipoxin A4 biosynthetic process
A	2001306	biological_process	lipoxin B4 biosynthetic process
B	0001676	biological_process	long-chain fatty acid metabolic process
B	0004052	molecular_function	arachidonate 12(S)-lipoxygenase activity
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0006631	biological_process	fatty acid metabolic process
B	0006690	biological_process	icosanoid metabolic process
B	0010656	biological_process	negative regulation of muscle cell apoptotic process
B	0016020	cellular_component	membrane
B	0016165	molecular_function	linoleate 13S-lipoxygenase activity
B	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
B	0016787	molecular_function	hydrolase activity
B	0019369	biological_process	arachidonate metabolic process
B	0019372	biological_process	lipoxygenase pathway
B	0019395	biological_process	fatty acid oxidation
B	0033559	biological_process	unsaturated fatty acid metabolic process
B	0034440	biological_process	lipid oxidation
B	0042383	cellular_component	sarcolemma
B	0042554	biological_process	superoxide anion generation
B	0043651	biological_process	linoleic acid metabolic process
B	0046872	molecular_function	metal ion binding
B	0047977	molecular_function	hepoxilin-epoxide hydrolase activity
B	0050473	molecular_function	arachidonate 15-lipoxygenase activity
B	0051122	biological_process	hepoxilin biosynthetic process
B	0051213	molecular_function	dioxygenase activity
B	0061436	biological_process	establishment of skin barrier
B	0070062	cellular_component	extracellular exosome
B	0090331	biological_process	negative regulation of platelet aggregation
B	1901751	biological_process	leukotriene A4 metabolic process
B	2001303	biological_process	lipoxin A4 biosynthetic process
B	2001306	biological_process	lipoxin B4 biosynthetic process
C	0001676	biological_process	long-chain fatty acid metabolic process
C	0004052	molecular_function	arachidonate 12(S)-lipoxygenase activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006629	biological_process	lipid metabolic process
C	0006631	biological_process	fatty acid metabolic process
C	0006690	biological_process	icosanoid metabolic process
C	0010656	biological_process	negative regulation of muscle cell apoptotic process
C	0016020	cellular_component	membrane
C	0016165	molecular_function	linoleate 13S-lipoxygenase activity
C	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
C	0016787	molecular_function	hydrolase activity
C	0019369	biological_process	arachidonate metabolic process
C	0019372	biological_process	lipoxygenase pathway
C	0019395	biological_process	fatty acid oxidation
C	0033559	biological_process	unsaturated fatty acid metabolic process
C	0034440	biological_process	lipid oxidation
C	0042383	cellular_component	sarcolemma
C	0042554	biological_process	superoxide anion generation
C	0043651	biological_process	linoleic acid metabolic process
C	0046872	molecular_function	metal ion binding
C	0047977	molecular_function	hepoxilin-epoxide hydrolase activity
C	0050473	molecular_function	arachidonate 15-lipoxygenase activity
C	0051122	biological_process	hepoxilin biosynthetic process
C	0051213	molecular_function	dioxygenase activity
C	0061436	biological_process	establishment of skin barrier
C	0070062	cellular_component	extracellular exosome
C	0090331	biological_process	negative regulation of platelet aggregation
C	1901751	biological_process	leukotriene A4 metabolic process
C	2001303	biological_process	lipoxin A4 biosynthetic process
C	2001306	biological_process	lipoxin B4 biosynthetic process
D	0001676	biological_process	long-chain fatty acid metabolic process
D	0004052	molecular_function	arachidonate 12(S)-lipoxygenase activity
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006629	biological_process	lipid metabolic process
D	0006631	biological_process	fatty acid metabolic process
D	0006690	biological_process	icosanoid metabolic process
D	0010656	biological_process	negative regulation of muscle cell apoptotic process
D	0016020	cellular_component	membrane
D	0016165	molecular_function	linoleate 13S-lipoxygenase activity
D	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
D	0016787	molecular_function	hydrolase activity
D	0019369	biological_process	arachidonate metabolic process
D	0019372	biological_process	lipoxygenase pathway
D	0019395	biological_process	fatty acid oxidation
D	0033559	biological_process	unsaturated fatty acid metabolic process
D	0034440	biological_process	lipid oxidation
D	0042383	cellular_component	sarcolemma
D	0042554	biological_process	superoxide anion generation
D	0043651	biological_process	linoleic acid metabolic process
D	0046872	molecular_function	metal ion binding
D	0047977	molecular_function	hepoxilin-epoxide hydrolase activity
D	0050473	molecular_function	arachidonate 15-lipoxygenase activity
D	0051122	biological_process	hepoxilin biosynthetic process
D	0051213	molecular_function	dioxygenase activity
D	0061436	biological_process	establishment of skin barrier
D	0070062	cellular_component	extracellular exosome
D	0090331	biological_process	negative regulation of platelet aggregation
D	1901751	biological_process	leukotriene A4 metabolic process
D	2001303	biological_process	lipoxin A4 biosynthetic process
D	2001306	biological_process	lipoxin B4 biosynthetic process
E	0001676	biological_process	long-chain fatty acid metabolic process
E	0004052	molecular_function	arachidonate 12(S)-lipoxygenase activity
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006629	biological_process	lipid metabolic process
E	0006631	biological_process	fatty acid metabolic process
E	0006690	biological_process	icosanoid metabolic process
E	0010656	biological_process	negative regulation of muscle cell apoptotic process
E	0016020	cellular_component	membrane
E	0016165	molecular_function	linoleate 13S-lipoxygenase activity
E	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
E	0016787	molecular_function	hydrolase activity
E	0019369	biological_process	arachidonate metabolic process
E	0019372	biological_process	lipoxygenase pathway
E	0019395	biological_process	fatty acid oxidation
E	0033559	biological_process	unsaturated fatty acid metabolic process
E	0034440	biological_process	lipid oxidation
E	0042383	cellular_component	sarcolemma
E	0042554	biological_process	superoxide anion generation
E	0043651	biological_process	linoleic acid metabolic process
E	0046872	molecular_function	metal ion binding
E	0047977	molecular_function	hepoxilin-epoxide hydrolase activity
E	0050473	molecular_function	arachidonate 15-lipoxygenase activity
E	0051122	biological_process	hepoxilin biosynthetic process
E	0051213	molecular_function	dioxygenase activity
E	0061436	biological_process	establishment of skin barrier
E	0070062	cellular_component	extracellular exosome
E	0090331	biological_process	negative regulation of platelet aggregation
E	1901751	biological_process	leukotriene A4 metabolic process
E	2001303	biological_process	lipoxin A4 biosynthetic process
E	2001306	biological_process	lipoxin B4 biosynthetic process
F	0001676	biological_process	long-chain fatty acid metabolic process
F	0004052	molecular_function	arachidonate 12(S)-lipoxygenase activity
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006629	biological_process	lipid metabolic process
F	0006631	biological_process	fatty acid metabolic process
F	0006690	biological_process	icosanoid metabolic process
F	0010656	biological_process	negative regulation of muscle cell apoptotic process
F	0016020	cellular_component	membrane
F	0016165	molecular_function	linoleate 13S-lipoxygenase activity
F	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
F	0016787	molecular_function	hydrolase activity
F	0019369	biological_process	arachidonate metabolic process
F	0019372	biological_process	lipoxygenase pathway
F	0019395	biological_process	fatty acid oxidation
F	0033559	biological_process	unsaturated fatty acid metabolic process
F	0034440	biological_process	lipid oxidation
F	0042383	cellular_component	sarcolemma
F	0042554	biological_process	superoxide anion generation
F	0043651	biological_process	linoleic acid metabolic process
F	0046872	molecular_function	metal ion binding
F	0047977	molecular_function	hepoxilin-epoxide hydrolase activity
F	0050473	molecular_function	arachidonate 15-lipoxygenase activity
F	0051122	biological_process	hepoxilin biosynthetic process
F	0051213	molecular_function	dioxygenase activity
F	0061436	biological_process	establishment of skin barrier
F	0070062	cellular_component	extracellular exosome
F	0090331	biological_process	negative regulation of platelet aggregation
F	1901751	biological_process	leukotriene A4 metabolic process
F	2001303	biological_process	lipoxin A4 biosynthetic process
F	2001306	biological_process	lipoxin B4 biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00081
Number of Residues	11
Details	LIPOXYGENASE_2 Lipoxygenases iron-binding region signature 2. LHPIfKFLiPH

Chain	Residue	Details
A	LEU382-HIS392

---

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. FTCTAQH

Chain	Residue	Details
A	PHE534-HIS540

---

site_id	PS00711
Number of Residues	15
Details	LIPOXYGENASE_1 Lipoxygenases iron-binding region signature 1. HEiqyHlLNTHLVaE

Chain	Residue	Details
A	HIS355-GLU369

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	BINDING:

Chain	Residue	Details
A	HIS360
D	HIS360
D	HIS365
D	HIS540
E	HIS360
E	HIS365
E	HIS540
F	HIS360
F	HIS365
F	HIS540
A	HIS365
A	HIS540
B	HIS360
B	HIS365
B	HIS540
C	HIS360
C	HIS365
C	HIS540

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00726

Chain	Residue	Details
A	ASN544
E	ILE663
F	ASN544
F	ILE663
A	ILE663
B	ASN544
B	ILE663
C	ASN544
C	ILE663
D	ASN544
D	ILE663
E	ASN544

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:F1LQ70

Chain	Residue	Details
A	SER246
B	SER246
C	SER246
D	SER246
E	SER246
F	SER246

---