8FY2
E3:PROTAC:target ternary complex structure (VCB/WH244/BCL-2)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| B | 0000122 | biological_process | negative regulation of transcription by RNA polymerase II |
| B | 0000151 | cellular_component | ubiquitin ligase complex |
| B | 0001222 | molecular_function | transcription corepressor binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006351 | biological_process | DNA-templated transcription |
| B | 0006367 | biological_process | transcription initiation at RNA polymerase II promoter |
| B | 0006368 | biological_process | transcription elongation by RNA polymerase II |
| B | 0016567 | biological_process | protein ubiquitination |
| B | 0030891 | cellular_component | VCB complex |
| B | 0031462 | cellular_component | Cul2-RING ubiquitin ligase complex |
| B | 0031466 | cellular_component | Cul5-RING ubiquitin ligase complex |
| B | 0031625 | molecular_function | ubiquitin protein ligase binding |
| B | 0032436 | biological_process | positive regulation of proteasomal ubiquitin-dependent protein catabolic process |
| B | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| B | 0065003 | biological_process | protein-containing complex assembly |
| B | 0070449 | cellular_component | elongin complex |
| B | 0140627 | biological_process | ubiquitin-dependent protein catabolic process via the C-end degron rule pathway |
| B | 0140958 | biological_process | target-directed miRNA degradation |
| B | 1990116 | biological_process | ribosome-associated ubiquitin-dependent protein catabolic process |
| B | 2000104 | biological_process | negative regulation of DNA-templated DNA replication |
| C | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0042981 | biological_process | regulation of apoptotic process |
| D | 0043066 | biological_process | negative regulation of apoptotic process |
Functional Information from PROSITE/UniProt
| site_id | PS01080 |
| Number of Residues | 19 |
| Details | BH1 Apoptosis regulator, Bcl-2 family BH1 motif signature. LFrDGv.NWGRIVAFFeFGG |
| Chain | Residue | Details |
| D | LEU137-GLY155 |
| site_id | PS01258 |
| Number of Residues | 12 |
| Details | BH2 Apoptosis regulator, Bcl-2 family BH2 motif signature. WIqdnGGWDaFV |
| Chain | Residue | Details |
| D | TRP188-VAL199 |
| site_id | PS01259 |
| Number of Residues | 15 |
| Details | BH3 Apoptosis regulator, Bcl-2 family BH3 motif signature. VhltLRqAGDDFSRR |
| Chain | Residue | Details |
| D | VAL93-ARG107 |
| site_id | PS01260 |
| Number of Residues | 21 |
| Details | BH4_1 Apoptosis regulator, Bcl-2 family BH4 motif signature. DNREIVmKYIHYKLSQRGYeW |
| Chain | Residue | Details |
| D | ASP10-TRP30 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 55 |
| Details | Region: {"description":"Involved in binding to CCT complex"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 9 |
| Details | Region: {"description":"Interaction with Elongin BC complex"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 65 |
| Details | Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62869","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 15 |
| Details | Region: {"description":"Required for interaction with SEPTIN4 isoform ARTS. Required XIAP-mediated ubiquitination and apoptosis","evidences":[{"source":"PubMed","id":"29020630","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 20 |
| Details | Motif: {"description":"BH4","evidences":[{"source":"PROSITE-ProRule","id":"PRU00025","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 14 |
| Details | Motif: {"description":"BH3","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 19 |
| Details | Motif: {"description":"BH1","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 15 |
| Details | Motif: {"description":"BH2","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by MAPK8","evidences":[{"source":"PubMed","id":"18570871","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
