8FPS

GluA2 flip Q isoform N619K mutant of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg/N619K)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0006811	biological_process	monoatomic ion transport
A	0015276	molecular_function	ligand-gated monoatomic ion channel activity
A	0016020	cellular_component	membrane
A	0038023	molecular_function	signaling receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0006811	biological_process	monoatomic ion transport
B	0015276	molecular_function	ligand-gated monoatomic ion channel activity
B	0016020	cellular_component	membrane
B	0038023	molecular_function	signaling receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0006811	biological_process	monoatomic ion transport
C	0015276	molecular_function	ligand-gated monoatomic ion channel activity
C	0016020	cellular_component	membrane
C	0038023	molecular_function	signaling receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0015276	molecular_function	ligand-gated monoatomic ion channel activity
D	0016020	cellular_component	membrane
D	0038023	molecular_function	signaling receptor activity
E	0005245	molecular_function	voltage-gated calcium channel activity
E	0005262	molecular_function	calcium channel activity
E	0005515	molecular_function	protein binding
E	0005891	cellular_component	voltage-gated calcium channel complex
E	0006612	biological_process	protein targeting to membrane
E	0006816	biological_process	calcium ion transport
E	0007528	biological_process	neuromuscular junction development
E	0009986	cellular_component	cell surface
E	0016020	cellular_component	membrane
E	0016247	molecular_function	channel regulator activity
E	0019226	biological_process	transmission of nerve impulse
E	0032281	cellular_component	AMPA glutamate receptor complex
E	0034702	cellular_component	monoatomic ion channel complex
E	0035255	molecular_function	ionotropic glutamate receptor binding
E	0036477	cellular_component	somatodendritic compartment
E	0042391	biological_process	regulation of membrane potential
E	0043005	cellular_component	neuron projection
E	0044300	cellular_component	cerebellar mossy fiber
E	0045202	cellular_component	synapse
E	0050877	biological_process	nervous system process
E	0051592	biological_process	response to calcium ion
E	0051899	biological_process	membrane depolarization
E	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
E	0060081	biological_process	membrane hyperpolarization
E	0060082	biological_process	eye blink reflex
E	0070588	biological_process	calcium ion transmembrane transport
E	0098685	cellular_component	Schaffer collateral - CA1 synapse
E	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
E	0098839	cellular_component	postsynaptic density membrane
E	0098943	biological_process	neurotransmitter receptor transport, postsynaptic endosome to lysosome
E	0098962	biological_process	regulation of postsynaptic neurotransmitter receptor activity
E	0098970	biological_process	postsynaptic neurotransmitter receptor diffusion trapping
E	0098978	cellular_component	glutamatergic synapse
E	0099072	biological_process	regulation of postsynaptic membrane neurotransmitter receptor levels
E	0099590	biological_process	neurotransmitter receptor internalization
E	0099645	biological_process	neurotransmitter receptor localization to postsynaptic specialization membrane
E	1904510	biological_process	positive regulation of protein localization to basolateral plasma membrane
E	2000311	biological_process	regulation of AMPA receptor activity
F	0005245	molecular_function	voltage-gated calcium channel activity
F	0005262	molecular_function	calcium channel activity
F	0005515	molecular_function	protein binding
F	0005891	cellular_component	voltage-gated calcium channel complex
F	0006612	biological_process	protein targeting to membrane
F	0006816	biological_process	calcium ion transport
F	0007528	biological_process	neuromuscular junction development
F	0009986	cellular_component	cell surface
F	0016020	cellular_component	membrane
F	0016247	molecular_function	channel regulator activity
F	0019226	biological_process	transmission of nerve impulse
F	0032281	cellular_component	AMPA glutamate receptor complex
F	0034702	cellular_component	monoatomic ion channel complex
F	0035255	molecular_function	ionotropic glutamate receptor binding
F	0036477	cellular_component	somatodendritic compartment
F	0042391	biological_process	regulation of membrane potential
F	0043005	cellular_component	neuron projection
F	0044300	cellular_component	cerebellar mossy fiber
F	0045202	cellular_component	synapse
F	0050877	biological_process	nervous system process
F	0051592	biological_process	response to calcium ion
F	0051899	biological_process	membrane depolarization
F	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
F	0060081	biological_process	membrane hyperpolarization
F	0060082	biological_process	eye blink reflex
F	0070588	biological_process	calcium ion transmembrane transport
F	0098685	cellular_component	Schaffer collateral - CA1 synapse
F	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
F	0098839	cellular_component	postsynaptic density membrane
F	0098943	biological_process	neurotransmitter receptor transport, postsynaptic endosome to lysosome
F	0098962	biological_process	regulation of postsynaptic neurotransmitter receptor activity
F	0098970	biological_process	postsynaptic neurotransmitter receptor diffusion trapping
F	0098978	cellular_component	glutamatergic synapse
F	0099072	biological_process	regulation of postsynaptic membrane neurotransmitter receptor levels
F	0099590	biological_process	neurotransmitter receptor internalization
F	0099645	biological_process	neurotransmitter receptor localization to postsynaptic specialization membrane
F	1904510	biological_process	positive regulation of protein localization to basolateral plasma membrane
F	2000311	biological_process	regulation of AMPA receptor activity
G	0005245	molecular_function	voltage-gated calcium channel activity
G	0005262	molecular_function	calcium channel activity
G	0005515	molecular_function	protein binding
G	0005891	cellular_component	voltage-gated calcium channel complex
G	0006612	biological_process	protein targeting to membrane
G	0006816	biological_process	calcium ion transport
G	0007528	biological_process	neuromuscular junction development
G	0009986	cellular_component	cell surface
G	0016020	cellular_component	membrane
G	0016247	molecular_function	channel regulator activity
G	0019226	biological_process	transmission of nerve impulse
G	0032281	cellular_component	AMPA glutamate receptor complex
G	0034702	cellular_component	monoatomic ion channel complex
G	0035255	molecular_function	ionotropic glutamate receptor binding
G	0036477	cellular_component	somatodendritic compartment
G	0042391	biological_process	regulation of membrane potential
G	0043005	cellular_component	neuron projection
G	0044300	cellular_component	cerebellar mossy fiber
G	0045202	cellular_component	synapse
G	0050877	biological_process	nervous system process
G	0051592	biological_process	response to calcium ion
G	0051899	biological_process	membrane depolarization
G	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
G	0060081	biological_process	membrane hyperpolarization
G	0060082	biological_process	eye blink reflex
G	0070588	biological_process	calcium ion transmembrane transport
G	0098685	cellular_component	Schaffer collateral - CA1 synapse
G	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
G	0098839	cellular_component	postsynaptic density membrane
G	0098943	biological_process	neurotransmitter receptor transport, postsynaptic endosome to lysosome
G	0098962	biological_process	regulation of postsynaptic neurotransmitter receptor activity
G	0098970	biological_process	postsynaptic neurotransmitter receptor diffusion trapping
G	0098978	cellular_component	glutamatergic synapse
G	0099072	biological_process	regulation of postsynaptic membrane neurotransmitter receptor levels
G	0099590	biological_process	neurotransmitter receptor internalization
G	0099645	biological_process	neurotransmitter receptor localization to postsynaptic specialization membrane
G	1904510	biological_process	positive regulation of protein localization to basolateral plasma membrane
G	2000311	biological_process	regulation of AMPA receptor activity
H	0005245	molecular_function	voltage-gated calcium channel activity
H	0005262	molecular_function	calcium channel activity
H	0005515	molecular_function	protein binding
H	0005891	cellular_component	voltage-gated calcium channel complex
H	0006612	biological_process	protein targeting to membrane
H	0006816	biological_process	calcium ion transport
H	0007528	biological_process	neuromuscular junction development
H	0009986	cellular_component	cell surface
H	0016020	cellular_component	membrane
H	0016247	molecular_function	channel regulator activity
H	0019226	biological_process	transmission of nerve impulse
H	0032281	cellular_component	AMPA glutamate receptor complex
H	0034702	cellular_component	monoatomic ion channel complex
H	0035255	molecular_function	ionotropic glutamate receptor binding
H	0036477	cellular_component	somatodendritic compartment
H	0042391	biological_process	regulation of membrane potential
H	0043005	cellular_component	neuron projection
H	0044300	cellular_component	cerebellar mossy fiber
H	0045202	cellular_component	synapse
H	0050877	biological_process	nervous system process
H	0051592	biological_process	response to calcium ion
H	0051899	biological_process	membrane depolarization
H	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
H	0060081	biological_process	membrane hyperpolarization
H	0060082	biological_process	eye blink reflex
H	0070588	biological_process	calcium ion transmembrane transport
H	0098685	cellular_component	Schaffer collateral - CA1 synapse
H	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
H	0098839	cellular_component	postsynaptic density membrane
H	0098943	biological_process	neurotransmitter receptor transport, postsynaptic endosome to lysosome
H	0098962	biological_process	regulation of postsynaptic neurotransmitter receptor activity
H	0098970	biological_process	postsynaptic neurotransmitter receptor diffusion trapping
H	0098978	cellular_component	glutamatergic synapse
H	0099072	biological_process	regulation of postsynaptic membrane neurotransmitter receptor levels
H	0099590	biological_process	neurotransmitter receptor internalization
H	0099645	biological_process	neurotransmitter receptor localization to postsynaptic specialization membrane
H	1904510	biological_process	positive regulation of protein localization to basolateral plasma membrane
H	2000311	biological_process	regulation of AMPA receptor activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	320
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
E	MET10-THR30
F	SER104-ALA124
F	ILE134-ILE154
F	PHE182-ILE202
H	MET10-THR30
H	SER104-ALA124
H	ILE134-ILE154
H	PHE182-ILE202
E	SER104-ALA124
E	ILE134-ILE154
E	PHE182-ILE202
G	MET10-THR30
G	SER104-ALA124
G	ILE134-ILE154
G	PHE182-ILE202
F	MET10-THR30

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q71RJ2

Chain	Residue	Details
E	SER253
G	SER253
F	SER253
H	SER253
C	TYR523-VAL543
C	LEU596-TYR616
D	TYR523-VAL543
D	LEU596-TYR616

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18034455

Chain	Residue	Details
E	TYR271
D	SER544-GLU570
D	ASP590-SER595
D	GLU813-GLY862
G	TYR271
F	TYR271
H	TYR271
B	ASP590-SER595
B	GLU813-GLY862
C	SER544-GLU570
C	ASP590-SER595
C	GLU813-GLY862

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by PKA => ECO:0000269|PubMed:11805122

Chain	Residue	Details
E	THR321
G	THR321
F	THR321
H	THR321

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
E	ASN48
G	ASN48
F	ASN48
H	ASN48

---

site_id	SWS_FT_FI6
Number of Residues	80
Details	TRANSMEM: Helical; Name=M4

Chain	Residue	Details
A	VAL792-ILE812
B	VAL792-ILE812
C	VAL792-ILE812
D	VAL792-ILE812

---

site_id	SWS_FT_FI7
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269|PubMed:11086992, ECO:0000269|PubMed:16483599, ECO:0007744|PDB:1FTJ, ECO:0007744|PDB:2CMO

Chain	Residue	Details
A	PRO478
B	SER654
B	THR655
B	GLU705
C	PRO478
C	THR480
C	ARG485
C	SER654
C	THR655
C	GLU705
D	PRO478
A	THR480
D	THR480
D	ARG485
D	SER654
D	THR655
D	GLU705
A	ARG485
A	SER654
A	THR655
A	GLU705
B	PRO478
B	THR480
B	ARG485

---

site_id	SWS_FT_FI8
Number of Residues	12
Details	SITE: Interaction with the cone snail toxin Con-ikot-ikot => ECO:0000269|PubMed:25103405

Chain	Residue	Details
A	ARG453
D	ARG453
D	ARG660
D	LYS752
A	ARG660
A	LYS752
B	ARG453
B	ARG660
B	LYS752
C	ARG453
C	ARG660
C	LYS752

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	SITE: Crucial to convey clamshell closure to channel opening => ECO:0000269|PubMed:25103405

Chain	Residue	Details
A	ILE633
B	ILE633
C	ILE633
D	ILE633

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:8848293

Chain	Residue	Details
A	SER662
B	SER662
C	SER662
D	SER662

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: Phosphoserine; by PKG => ECO:0000269|PubMed:8848293

Chain	Residue	Details
A	SER696
B	SER696
C	SER696
D	SER696

---

site_id	SWS_FT_FI12
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P23819

Chain	Residue	Details
A	SER839
D	SER839
D	SER842
D	SER865
A	SER842
A	SER865
B	SER839
B	SER842
B	SER865
C	SER839
C	SER842
C	SER865

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:15240807, ECO:0000269|PubMed:20547133

Chain	Residue	Details
A	TYR861
B	TYR861
C	TYR861
D	TYR861

---

site_id	SWS_FT_FI14
Number of Residues	8
Details	LIPID: S-palmitoyl cysteine => ECO:0000250

Chain	Residue	Details
A	CYS589
A	CYS815
B	CYS589
B	CYS815
C	CYS589
C	CYS815
D	CYS589
D	CYS815

---

site_id	SWS_FT_FI15
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:21317873

Chain	Residue	Details
A	ASN235
B	ASN235
C	ASN235
D	ASN235

---

site_id	SWS_FT_FI16
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19946266, ECO:0000269|PubMed:21317873, ECO:0000269|PubMed:25103405

Chain	Residue	Details
A	ASN349
B	ASN349
C	ASN349
D	ASN349

---

site_id	SWS_FT_FI17
Number of Residues	8
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN385
A	ASN392
B	ASN385
B	ASN392
C	ASN385
C	ASN392
D	ASN385
D	ASN392

---