8FJ0

Crystal Structure of the Tick Evasin EVA-AAM1001(Y44A) Complexed to Human Chemokine CCL2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0019957	molecular_function	C-C chemokine binding
A	1900137	biological_process	negative regulation of chemokine activity
B	0019957	molecular_function	C-C chemokine binding
B	1900137	biological_process	negative regulation of chemokine activity
C	0019957	molecular_function	C-C chemokine binding
C	1900137	biological_process	negative regulation of chemokine activity
D	0001525	biological_process	angiogenesis
D	0002548	biological_process	monocyte chemotaxis
D	0004672	molecular_function	protein kinase activity
D	0005102	molecular_function	signaling receptor binding
D	0005125	molecular_function	cytokine activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0006468	biological_process	protein phosphorylation
D	0006935	biological_process	chemotaxis
D	0006954	biological_process	inflammatory response
D	0006955	biological_process	immune response
D	0006959	biological_process	humoral immune response
D	0007010	biological_process	cytoskeleton organization
D	0007155	biological_process	cell adhesion
D	0007165	biological_process	signal transduction
D	0007166	biological_process	cell surface receptor signaling pathway
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007187	biological_process	G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
D	0007259	biological_process	cell surface receptor signaling pathway via JAK-STAT
D	0008009	molecular_function	chemokine activity
D	0008360	biological_process	regulation of cell shape
D	0009617	biological_process	response to bacterium
D	0009887	biological_process	animal organ morphogenesis
D	0019079	biological_process	viral genome replication
D	0019221	biological_process	cytokine-mediated signaling pathway
D	0019233	biological_process	sensory perception of pain
D	0019725	biological_process	cellular homeostasis
D	0030335	biological_process	positive regulation of cell migration
D	0031727	molecular_function	CCR2 chemokine receptor binding
D	0034351	biological_process	negative regulation of glial cell apoptotic process
D	0035684	biological_process	helper T cell extravasation
D	0043524	biological_process	negative regulation of neuron apoptotic process
D	0043615	biological_process	astrocyte cell migration
D	0044344	biological_process	cellular response to fibroblast growth factor stimulus
D	0048020	molecular_function	CCR chemokine receptor binding
D	0048245	biological_process	eosinophil chemotaxis
D	0048246	biological_process	macrophage chemotaxis
D	0050870	biological_process	positive regulation of T cell activation
D	0051770	biological_process	positive regulation of nitric-oxide synthase biosynthetic process
D	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
D	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
D	0070098	biological_process	chemokine-mediated signaling pathway
D	0071222	biological_process	cellular response to lipopolysaccharide
D	0071346	biological_process	cellular response to type II interferon
D	0071347	biological_process	cellular response to interleukin-1
D	0071356	biological_process	cellular response to tumor necrosis factor
D	0090280	biological_process	positive regulation of calcium ion import
D	1904783	biological_process	positive regulation of NMDA glutamate receptor activity
D	1905563	biological_process	negative regulation of vascular endothelial cell proliferation
D	2000134	biological_process	negative regulation of G1/S transition of mitotic cell cycle
D	2000353	biological_process	positive regulation of endothelial cell apoptotic process
D	2000427	biological_process	positive regulation of apoptotic cell clearance
D	2000502	biological_process	negative regulation of natural killer cell chemotaxis
E	0001525	biological_process	angiogenesis
E	0002548	biological_process	monocyte chemotaxis
E	0004672	molecular_function	protein kinase activity
E	0005102	molecular_function	signaling receptor binding
E	0005125	molecular_function	cytokine activity
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0006468	biological_process	protein phosphorylation
E	0006935	biological_process	chemotaxis
E	0006954	biological_process	inflammatory response
E	0006955	biological_process	immune response
E	0006959	biological_process	humoral immune response
E	0007010	biological_process	cytoskeleton organization
E	0007155	biological_process	cell adhesion
E	0007165	biological_process	signal transduction
E	0007166	biological_process	cell surface receptor signaling pathway
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0007187	biological_process	G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
E	0007259	biological_process	cell surface receptor signaling pathway via JAK-STAT
E	0008009	molecular_function	chemokine activity
E	0008360	biological_process	regulation of cell shape
E	0009617	biological_process	response to bacterium
E	0009887	biological_process	animal organ morphogenesis
E	0019079	biological_process	viral genome replication
E	0019221	biological_process	cytokine-mediated signaling pathway
E	0019233	biological_process	sensory perception of pain
E	0019725	biological_process	cellular homeostasis
E	0030335	biological_process	positive regulation of cell migration
E	0031727	molecular_function	CCR2 chemokine receptor binding
E	0034351	biological_process	negative regulation of glial cell apoptotic process
E	0035684	biological_process	helper T cell extravasation
E	0043524	biological_process	negative regulation of neuron apoptotic process
E	0043615	biological_process	astrocyte cell migration
E	0044344	biological_process	cellular response to fibroblast growth factor stimulus
E	0048020	molecular_function	CCR chemokine receptor binding
E	0048245	biological_process	eosinophil chemotaxis
E	0048246	biological_process	macrophage chemotaxis
E	0050870	biological_process	positive regulation of T cell activation
E	0051770	biological_process	positive regulation of nitric-oxide synthase biosynthetic process
E	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
E	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
E	0070098	biological_process	chemokine-mediated signaling pathway
E	0071222	biological_process	cellular response to lipopolysaccharide
E	0071346	biological_process	cellular response to type II interferon
E	0071347	biological_process	cellular response to interleukin-1
E	0071356	biological_process	cellular response to tumor necrosis factor
E	0090280	biological_process	positive regulation of calcium ion import
E	1904783	biological_process	positive regulation of NMDA glutamate receptor activity
E	1905563	biological_process	negative regulation of vascular endothelial cell proliferation
E	2000134	biological_process	negative regulation of G1/S transition of mitotic cell cycle
E	2000353	biological_process	positive regulation of endothelial cell apoptotic process
E	2000427	biological_process	positive regulation of apoptotic cell clearance
E	2000502	biological_process	negative regulation of natural killer cell chemotaxis
F	0001525	biological_process	angiogenesis
F	0002548	biological_process	monocyte chemotaxis
F	0004672	molecular_function	protein kinase activity
F	0005102	molecular_function	signaling receptor binding
F	0005125	molecular_function	cytokine activity
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0006468	biological_process	protein phosphorylation
F	0006935	biological_process	chemotaxis
F	0006954	biological_process	inflammatory response
F	0006955	biological_process	immune response
F	0006959	biological_process	humoral immune response
F	0007010	biological_process	cytoskeleton organization
F	0007155	biological_process	cell adhesion
F	0007165	biological_process	signal transduction
F	0007166	biological_process	cell surface receptor signaling pathway
F	0007186	biological_process	G protein-coupled receptor signaling pathway
F	0007187	biological_process	G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
F	0007259	biological_process	cell surface receptor signaling pathway via JAK-STAT
F	0008009	molecular_function	chemokine activity
F	0008360	biological_process	regulation of cell shape
F	0009617	biological_process	response to bacterium
F	0009887	biological_process	animal organ morphogenesis
F	0019079	biological_process	viral genome replication
F	0019221	biological_process	cytokine-mediated signaling pathway
F	0019233	biological_process	sensory perception of pain
F	0019725	biological_process	cellular homeostasis
F	0030335	biological_process	positive regulation of cell migration
F	0031727	molecular_function	CCR2 chemokine receptor binding
F	0034351	biological_process	negative regulation of glial cell apoptotic process
F	0035684	biological_process	helper T cell extravasation
F	0043524	biological_process	negative regulation of neuron apoptotic process
F	0043615	biological_process	astrocyte cell migration
F	0044344	biological_process	cellular response to fibroblast growth factor stimulus
F	0048020	molecular_function	CCR chemokine receptor binding
F	0048245	biological_process	eosinophil chemotaxis
F	0048246	biological_process	macrophage chemotaxis
F	0050870	biological_process	positive regulation of T cell activation
F	0051770	biological_process	positive regulation of nitric-oxide synthase biosynthetic process
F	0051968	biological_process	positive regulation of synaptic transmission, glutamatergic
F	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
F	0070098	biological_process	chemokine-mediated signaling pathway
F	0071222	biological_process	cellular response to lipopolysaccharide
F	0071346	biological_process	cellular response to type II interferon
F	0071347	biological_process	cellular response to interleukin-1
F	0071356	biological_process	cellular response to tumor necrosis factor
F	0090280	biological_process	positive regulation of calcium ion import
F	1904783	biological_process	positive regulation of NMDA glutamate receptor activity
F	1905563	biological_process	negative regulation of vascular endothelial cell proliferation
F	2000134	biological_process	negative regulation of G1/S transition of mitotic cell cycle
F	2000353	biological_process	positive regulation of endothelial cell apoptotic process
F	2000427	biological_process	positive regulation of apoptotic cell clearance
F	2000502	biological_process	negative regulation of natural killer cell chemotaxis

Functional Information from PROSITE/UniProt

site_id	PS00472
Number of Residues	43
Details	SMALL_CYTOKINES_CC Small cytokines (intercrine/chemokine) C-C subfamily signature. CCYnftnrk..IsvqrLasYrritssk.Cpke.AVIFktivakei.CA

Chain	Residue	Details
D	CYS11-ALA53

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	9
Details	SITE: Involved in dimerization

Chain	Residue	Details
D	ALA8
B	ASN12
B	ASN32
B	ASN35
B	ASN49
B	ASN63
B	ASN77
B	ASN93
C	ASN7
C	ASN12
C	ASN32
D	LYS35
C	ASN35
C	ASN49
C	ASN63
C	ASN77
C	ASN93
D	LYS38
F	ALA8
F	LYS35
F	LYS38
E	ALA8
E	LYS35
E	LYS38

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site_id	SWS_FT_FI2
Number of Residues	3
Details	SITE: Involved in dimerization, receptor binding and signaling

Chain	Residue	Details
D	TYR13
F	TYR13
E	TYR13

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site_id	SWS_FT_FI3
Number of Residues	12
Details	SITE: Involved in GAG binding

Chain	Residue	Details
D	ARG18
E	LYS19
E	LYS58
E	HIS66
D	LYS19
D	LYS58
D	HIS66
F	ARG18
F	LYS19
F	LYS58
F	HIS66
E	ARG18

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site_id	SWS_FT_FI4
Number of Residues	6
Details	SITE: Involved in GAG binding and receptor binding

Chain	Residue	Details
D	ARG24
D	LYS49
F	ARG24
F	LYS49
E	ARG24
E	LYS49

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site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: Pyrrolidone carboxylic acid => ECO:0000269|PubMed:1661560, ECO:0000269|PubMed:2648385

Chain	Residue	Details
D	GLN1
F	GLN1
E	GLN1

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site_id	SWS_FT_FI6
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
D	ASN14
F	ASN14
E	ASN14

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