8FHT
Cryo-EM structure of human NCC
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0006814 | biological_process | sodium ion transport |
| A | 0006884 | biological_process | cell volume homeostasis |
| A | 0008511 | molecular_function | sodium:potassium:chloride symporter activity |
| A | 0015081 | molecular_function | sodium ion transmembrane transporter activity |
| A | 0015293 | molecular_function | symporter activity |
| A | 0015377 | molecular_function | chloride:monoatomic cation symporter activity |
| A | 0015378 | molecular_function | sodium:chloride symporter activity |
| A | 0016020 | cellular_component | membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0035725 | biological_process | sodium ion transmembrane transport |
| A | 0055064 | biological_process | chloride ion homeostasis |
| A | 0055075 | biological_process | potassium ion homeostasis |
| A | 0055078 | biological_process | sodium ion homeostasis |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070294 | biological_process | renal sodium ion absorption |
| A | 1902074 | biological_process | response to salt |
| A | 1902476 | biological_process | chloride transmembrane transport |
| A | 1904044 | biological_process | response to aldosterone |
| A | 1990573 | biological_process | potassium ion import across plasma membrane |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0006814 | biological_process | sodium ion transport |
| B | 0006884 | biological_process | cell volume homeostasis |
| B | 0008511 | molecular_function | sodium:potassium:chloride symporter activity |
| B | 0015081 | molecular_function | sodium ion transmembrane transporter activity |
| B | 0015293 | molecular_function | symporter activity |
| B | 0015377 | molecular_function | chloride:monoatomic cation symporter activity |
| B | 0015378 | molecular_function | sodium:chloride symporter activity |
| B | 0016020 | cellular_component | membrane |
| B | 0016324 | cellular_component | apical plasma membrane |
| B | 0022857 | molecular_function | transmembrane transporter activity |
| B | 0035725 | biological_process | sodium ion transmembrane transport |
| B | 0055064 | biological_process | chloride ion homeostasis |
| B | 0055075 | biological_process | potassium ion homeostasis |
| B | 0055078 | biological_process | sodium ion homeostasis |
| B | 0055085 | biological_process | transmembrane transport |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0070294 | biological_process | renal sodium ion absorption |
| B | 1902074 | biological_process | response to salt |
| B | 1902476 | biological_process | chloride transmembrane transport |
| B | 1904044 | biological_process | response to aldosterone |
| B | 1990573 | biological_process | potassium ion import across plasma membrane |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1296 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | MET1-GLY137 | |
| B | LYS478-ARG507 | |
| B | PHE545-LYS567 | |
| B | LEU600-GLN1021 | |
| A | SER190-GLY220 | |
| A | SER361-ILE371 | |
| A | LYS478-ARG507 | |
| A | PHE545-LYS567 | |
| A | LEU600-GLN1021 | |
| B | MET1-GLY137 | |
| B | SER190-GLY220 | |
| B | SER361-ILE371 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 58 |
| Details | TRANSMEM: Discontinuously helical; Name=1 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | TRP138-GLY167 | |
| B | TRP138-GLY167 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ILE168-ILE189 | |
| B | ILE168-ILE189 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | LEU221-ARG243 | |
| B | LEU221-ARG243 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 148 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ASP244-ASP255 | |
| A | GLY394-PHE453 | |
| A | ALA523-THR527 | |
| B | ASP244-ASP255 | |
| B | GLY394-PHE453 | |
| B | ALA523-THR527 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | PRO256-GLU280 | |
| B | PRO256-GLU280 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | TRP281-GLY303 | |
| B | TRP281-GLY303 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 68 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0000305, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | THR304-GLY338 | |
| B | THR304-GLY338 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 42 |
| Details | TRANSMEM: Discontinuously helical; Name=6 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | THR339-ILE360 | |
| B | THR339-ILE360 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | PRO372-ILE393 | |
| B | PRO372-ILE393 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=8 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ALA454-ALA477 | |
| B | ALA454-ALA477 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=9 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | GLY508-ILE522 | |
| B | GLY508-ILE522 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 32 |
| Details | TRANSMEM: Helical; Name=10 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ILE528-ASN544 | |
| B | ILE528-ASN544 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 38 |
| Details | TRANSMEM: Helical; Name=11 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | TRP568-TRP587 | |
| B | TRP568-TRP587 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=12 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ALA588-LEU599 | |
| B | ALA588-LEU599 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | LEU148 | |
| B | SER468 | |
| A | TRP151 | |
| A | ALA464 | |
| A | SER467 | |
| A | SER468 | |
| B | LEU148 | |
| B | TRP151 | |
| B | ALA464 | |
| B | SER467 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR |
| Chain | Residue | Details |
| A | ASN149 | |
| B | ASN149 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ |
| Chain | Residue | Details |
| A | ASN227 | |
| B | ASN227 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR |
| Chain | Residue | Details |
| A | HIS234 | |
| B | HIS234 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHR |
| Chain | Residue | Details |
| A | THR352 | |
| B | THR352 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:36351028, ECO:0000305|PubMed:36792826, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | GLY353 | |
| A | ILE354 | |
| A | LEU355 | |
| A | TYR540 | |
| B | GLY353 | |
| B | ILE354 | |
| B | LEU355 | |
| B | TYR540 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN |
| Chain | Residue | Details |
| A | ASN359 | |
| B | ASN359 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN, ECO:0007744|PDB:8FHO |
| Chain | Residue | Details |
| A | LEU648 | |
| B | GLY741 | |
| B | LEU780 | |
| B | ASN781 | |
| A | ARG655 | |
| A | VAL677 | |
| A | GLY741 | |
| A | LEU780 | |
| A | ASN781 | |
| B | LEU648 | |
| B | ARG655 | |
| B | VAL677 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P59158 |
| Chain | Residue | Details |
| A | SER43 | |
| A | SER49 | |
| A | ASP73 | |
| A | SER126 | |
| B | SER43 | |
| B | SER49 | |
| B | ASP73 | |
| B | SER126 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphothreonine; by OXSR1 and STK39 => ECO:0000269|PubMed:18270262 |
| Chain | Residue | Details |
| A | THR46 | |
| A | ASP55 | |
| A | ASP60 | |
| B | THR46 | |
| B | ASP55 | |
| B | ASP60 |
| site_id | SWS_FT_FI26 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P59158 |
| Chain | Residue | Details |
| A | THR50 | |
| A | THR124 | |
| B | THR50 | |
| B | THR124 |
| site_id | SWS_FT_FI27 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by OXSR1 and STK39 => ECO:0000305|PubMed:18270262, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER91 | |
| B | SER91 |
| site_id | SWS_FT_FI28 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:36351028, ECO:0007744|PDB:7Y6I |
| Chain | Residue | Details |
| A | ASN406 | |
| A | ASN426 | |
| B | ASN406 | |
| B | ASN426 |
