Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8FHT

Cryo-EM structure of human NCC

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002021	biological_process	response to dietary excess
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0006814	biological_process	sodium ion transport
A	0006884	biological_process	cell volume homeostasis
A	0007165	biological_process	signal transduction
A	0008511	molecular_function	sodium:potassium:chloride symporter activity
A	0015081	molecular_function	sodium ion transmembrane transporter activity
A	0015293	molecular_function	symporter activity
A	0015377	molecular_function	chloride:monoatomic cation symporter activity
A	0015378	molecular_function	sodium:chloride symporter activity
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0035725	biological_process	sodium ion transmembrane transport
A	0046873	molecular_function	metal ion transmembrane transporter activity
A	0055064	biological_process	chloride ion homeostasis
A	0055075	biological_process	potassium ion homeostasis
A	0055078	biological_process	sodium ion homeostasis
A	0055085	biological_process	transmembrane transport
A	0070062	cellular_component	extracellular exosome
A	0070294	biological_process	renal sodium ion absorption
A	0071241	biological_process	obsolete cellular response to inorganic substance
A	1902476	biological_process	chloride transmembrane transport
A	1904044	biological_process	response to aldosterone
A	1990573	biological_process	potassium ion import across plasma membrane
B	0002021	biological_process	response to dietary excess
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006811	biological_process	monoatomic ion transport
B	0006814	biological_process	sodium ion transport
B	0006884	biological_process	cell volume homeostasis
B	0007165	biological_process	signal transduction
B	0008511	molecular_function	sodium:potassium:chloride symporter activity
B	0015081	molecular_function	sodium ion transmembrane transporter activity
B	0015293	molecular_function	symporter activity
B	0015377	molecular_function	chloride:monoatomic cation symporter activity
B	0015378	molecular_function	sodium:chloride symporter activity
B	0016020	cellular_component	membrane
B	0016324	cellular_component	apical plasma membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0035725	biological_process	sodium ion transmembrane transport
B	0046873	molecular_function	metal ion transmembrane transporter activity
B	0055064	biological_process	chloride ion homeostasis
B	0055075	biological_process	potassium ion homeostasis
B	0055078	biological_process	sodium ion homeostasis
B	0055085	biological_process	transmembrane transport
B	0070062	cellular_component	extracellular exosome
B	0070294	biological_process	renal sodium ion absorption
B	0071241	biological_process	obsolete cellular response to inorganic substance
B	1902476	biological_process	chloride transmembrane transport
B	1904044	biological_process	response to aldosterone
B	1990573	biological_process	potassium ion import across plasma membrane

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1206
Details	TOPO_DOM: Cytoplasmic => ECO:0000269\|PubMed:36351028, ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:7Y6I, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR, ECO:0007744\|PDB:8FHT

Chain	Residue	Details
A	MET1-GLY137
B	GLY278
B	SER361-ASP366
B	ASP486-PRO505
B	SER555-TYR565
B	LYS607-GLN1021
A	THR194-GLY212
A	GLY278
A	SER361-ASP366
A	ASP486-PRO505
A	SER555-TYR565
A	LYS607-GLN1021
B	MET1-GLY137
B	THR194-GLY212

site_id	SWS_FT_FI2
Number of Residues	56
Details	TRANSMEM: Discontinuously helical => ECO:0000269\|PubMed:36351028, ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:7Y6I, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR, ECO:0007744\|PDB:8FHT

Chain	Residue	Details
A	TRP138-ALA166
B	TRP138-ALA166

site_id	SWS_FT_FI3
Number of Residues	198
Details	TOPO_DOM: Extracellular => ECO:0000269\|PubMed:36351028, ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:7Y6I, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR, ECO:0007744\|PDB:8FHT

Chain	Residue	Details
A	GLY167
B	VAL397-PHE453
B	GLU524-LEU525
B	THR585
A	GLY250-ILE257
A	ILE306-THR339
A	VAL397-PHE453
A	GLU524-LEU525
A	THR585
B	GLY167
B	GLY250-ILE257
B	ILE306-THR339

site_id	SWS_FT_FI4
Number of Residues	538
Details	TRANSMEM: Helical => ECO:0000269\|PubMed:36351028, ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:7Y6I, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR, ECO:0007744\|PDB:8FHT

Chain	Residue	Details
A	ILE168-SER193
A	ASN566-LEU584
A	TRP586-LYS606
B	ILE168-SER193
B	PRO213-TYR249
B	ASN258-ALA277
B	MET279-LEU305
B	PHE340-ILE360
B	PRO367-CYS396
B	ALA454-GLU485
B	VAL506-ALA523
A	PRO213-TYR249
B	ASN526-ASN554
B	ASN566-LEU584
B	TRP586-LYS606
A	ASN258-ALA277
A	MET279-LEU305
A	PHE340-ILE360
A	PRO367-CYS396
A	ALA454-GLU485
A	VAL506-ALA523
A	ASN526-ASN554

site_id	SWS_FT_FI5
Number of Residues	10
Details	BINDING: BINDING => ECO:0000269\|PubMed:36351028, ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:7Y6I, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR, ECO:0007744\|PDB:8FHT

Chain	Residue	Details
A	LEU148
B	SER468
A	TRP151
A	ALA464
A	SER467
A	SER468
B	LEU148
B	TRP151
B	ALA464
B	SER467

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR

Chain	Residue	Details
A	ASN149
B	ASN149

site_id	SWS_FT_FI7
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ

Chain	Residue	Details
A	ASN227
B	ASN227

site_id	SWS_FT_FI8
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHO, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHQ, ECO:0007744\|PDB:8FHR

Chain	Residue	Details
A	HIS234
B	HIS234

site_id	SWS_FT_FI9
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHP, ECO:0007744\|PDB:8FHR

Chain	Residue	Details
A	THR352
B	THR352

site_id	SWS_FT_FI10
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305\|PubMed:36351028, ECO:0000305\|PubMed:36792826

Chain	Residue	Details
A	GLY353
A	ILE354
A	LEU355
A	TYR540
B	GLY353
B	ILE354
B	LEU355
B	TYR540

site_id	SWS_FT_FI11
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:8FHN

Chain	Residue	Details
A	ASN359
B	ASN359

site_id	SWS_FT_FI12
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:36792826, ECO:0007744\|PDB:8FHN, ECO:0007744\|PDB:8FHO

Chain	Residue	Details
A	LEU648
B	GLY741
B	LEU780
B	ASN781
A	ARG655
A	VAL677
A	GLY741
A	LEU780
A	ASN781
B	LEU648
B	ARG655
B	VAL677

site_id	SWS_FT_FI13
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P59158

Chain	Residue	Details
A	SER43
A	SER49
A	ASP73
A	SER126
B	SER43
B	SER49
B	ASP73
B	SER126

site_id	SWS_FT_FI14
Number of Residues	6
Details	MOD_RES: Phosphothreonine; by OXSR1 and STK39 => ECO:0000269\|PubMed:18270262

Chain	Residue	Details
A	THR46
A	ASP55
A	ASP60
B	THR46
B	ASP55
B	ASP60

site_id	SWS_FT_FI15
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P59158

Chain	Residue	Details
A	THR50
A	THR124
B	THR50
B	THR124

site_id	SWS_FT_FI16
Number of Residues	2
Details	MOD_RES: Phosphoserine; by OXSR1 and STK39 => ECO:0000305\|PubMed:18270262, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER91
B	SER91

site_id	SWS_FT_FI17
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:36351028, ECO:0007744\|PDB:7Y6I

Chain	Residue	Details
A	ASN406
A	ASN426
B	ASN406
B	ASN426

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)