8EWV

DNA-encoded library (DEL)-enabled discovery of proximity inducing small molecules

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000151	cellular_component	ubiquitin ligase complex
A	0001222	molecular_function	transcription corepressor binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006351	biological_process	DNA-templated transcription
A	0006367	biological_process	transcription initiation at RNA polymerase II promoter
A	0006368	biological_process	transcription elongation by RNA polymerase II
A	0016567	biological_process	protein ubiquitination
A	0030891	cellular_component	VCB complex
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0065003	biological_process	protein-containing complex assembly
A	0070449	cellular_component	elongin complex
A	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A	0140958	biological_process	target-directed miRNA degradation
A	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
A	2000104	biological_process	negative regulation of DNA-templated DNA replication
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0000151	cellular_component	ubiquitin ligase complex
E	0001222	molecular_function	transcription corepressor binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006351	biological_process	DNA-templated transcription
E	0006367	biological_process	transcription initiation at RNA polymerase II promoter
E	0006368	biological_process	transcription elongation by RNA polymerase II
E	0016567	biological_process	protein ubiquitination
E	0030891	cellular_component	VCB complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0065003	biological_process	protein-containing complex assembly
E	0070449	cellular_component	elongin complex
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0140958	biological_process	target-directed miRNA degradation
E	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
E	2000104	biological_process	negative regulation of DNA-templated DNA replication
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
I	0000122	biological_process	negative regulation of transcription by RNA polymerase II
I	0000151	cellular_component	ubiquitin ligase complex
I	0001222	molecular_function	transcription corepressor binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006351	biological_process	DNA-templated transcription
I	0006367	biological_process	transcription initiation at RNA polymerase II promoter
I	0006368	biological_process	transcription elongation by RNA polymerase II
I	0016567	biological_process	protein ubiquitination
I	0030891	cellular_component	VCB complex
I	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
I	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
I	0031625	molecular_function	ubiquitin protein ligase binding
I	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0065003	biological_process	protein-containing complex assembly
I	0070449	cellular_component	elongin complex
I	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
I	0140958	biological_process	target-directed miRNA degradation
I	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
I	2000104	biological_process	negative regulation of DNA-templated DNA replication
J	0006511	biological_process	ubiquitin-dependent protein catabolic process
M	0000122	biological_process	negative regulation of transcription by RNA polymerase II
M	0000151	cellular_component	ubiquitin ligase complex
M	0001222	molecular_function	transcription corepressor binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006351	biological_process	DNA-templated transcription
M	0006367	biological_process	transcription initiation at RNA polymerase II promoter
M	0006368	biological_process	transcription elongation by RNA polymerase II
M	0016567	biological_process	protein ubiquitination
M	0030891	cellular_component	VCB complex
M	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
M	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
M	0031625	molecular_function	ubiquitin protein ligase binding
M	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0065003	biological_process	protein-containing complex assembly
M	0070449	cellular_component	elongin complex
M	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
M	0140958	biological_process	target-directed miRNA degradation
M	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
M	2000104	biological_process	negative regulation of DNA-templated DNA replication
N	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0000122	biological_process	negative regulation of transcription by RNA polymerase II
Q	0000151	cellular_component	ubiquitin ligase complex
Q	0001222	molecular_function	transcription corepressor binding
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006351	biological_process	DNA-templated transcription
Q	0006367	biological_process	transcription initiation at RNA polymerase II promoter
Q	0006368	biological_process	transcription elongation by RNA polymerase II
Q	0016567	biological_process	protein ubiquitination
Q	0030891	cellular_component	VCB complex
Q	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
Q	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
Q	0031625	molecular_function	ubiquitin protein ligase binding
Q	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0065003	biological_process	protein-containing complex assembly
Q	0070449	cellular_component	elongin complex
Q	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
Q	0140958	biological_process	target-directed miRNA degradation
Q	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
Q	2000104	biological_process	negative regulation of DNA-templated DNA replication
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0000122	biological_process	negative regulation of transcription by RNA polymerase II
U	0000151	cellular_component	ubiquitin ligase complex
U	0001222	molecular_function	transcription corepressor binding
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0006351	biological_process	DNA-templated transcription
U	0006367	biological_process	transcription initiation at RNA polymerase II promoter
U	0006368	biological_process	transcription elongation by RNA polymerase II
U	0016567	biological_process	protein ubiquitination
U	0030891	cellular_component	VCB complex
U	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
U	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
U	0031625	molecular_function	ubiquitin protein ligase binding
U	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0065003	biological_process	protein-containing complex assembly
U	0070449	cellular_component	elongin complex
U	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
U	0140958	biological_process	target-directed miRNA degradation
U	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
U	2000104	biological_process	negative regulation of DNA-templated DNA replication
V	0006511	biological_process	ubiquitin-dependent protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00633
Number of Residues	60
Details	BROMODOMAIN_1 Bromodomain signature. AwpFqqpvDavklnlpDYYkiIktpMdmgtIkkrlenny..Ywnaqeciqdfnt.MftNCyiY

Chain	Residue	Details
D	ALA80-TYR139

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	390
Details	Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62869","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	330
Details	Region: {"description":"Involved in binding to CCT complex"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	54
Details	Region: {"description":"Interaction with Elongin BC complex"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Site: {"description":"Acetylated histone binding","evidences":[{"source":"PubMed","id":"22464331","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---