8EP1

Eag Kv channel with voltage sensor in the down conformation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0005249	molecular_function	voltage-gated potassium channel activity
A	0006811	biological_process	monoatomic ion transport
A	0006813	biological_process	potassium ion transport
A	0016020	cellular_component	membrane
A	0055085	biological_process	transmembrane transport
B	0005216	molecular_function	monoatomic ion channel activity
B	0005249	molecular_function	voltage-gated potassium channel activity
B	0006811	biological_process	monoatomic ion transport
B	0006813	biological_process	potassium ion transport
B	0016020	cellular_component	membrane
B	0055085	biological_process	transmembrane transport
C	0005216	molecular_function	monoatomic ion channel activity
C	0005249	molecular_function	voltage-gated potassium channel activity
C	0006811	biological_process	monoatomic ion transport
C	0006813	biological_process	potassium ion transport
C	0016020	cellular_component	membrane
C	0055085	biological_process	transmembrane transport
D	0005216	molecular_function	monoatomic ion channel activity
D	0005249	molecular_function	voltage-gated potassium channel activity
D	0006811	biological_process	monoatomic ion transport
D	0006813	biological_process	potassium ion transport
D	0016020	cellular_component	membrane
D	0055085	biological_process	transmembrane transport
E	0000086	biological_process	G2/M transition of mitotic cell cycle
E	0000922	cellular_component	spindle pole
E	0002027	biological_process	regulation of heart rate
E	0005509	molecular_function	calcium ion binding
E	0005513	biological_process	detection of calcium ion
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005819	cellular_component	spindle
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005876	cellular_component	spindle microtubule
E	0005886	cellular_component	plasma membrane
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0008076	cellular_component	voltage-gated potassium channel complex
E	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
E	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
E	0010856	molecular_function	adenylate cyclase activator activity
E	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
E	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
E	0016020	cellular_component	membrane
E	0016240	biological_process	autophagosome membrane docking
E	0019855	molecular_function	calcium channel inhibitor activity
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0030017	cellular_component	sarcomere
E	0031432	molecular_function	titin binding
E	0031514	cellular_component	motile cilium
E	0031954	biological_process	positive regulation of protein autophosphorylation
E	0031982	cellular_component	vesicle
E	0032465	biological_process	regulation of cytokinesis
E	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
E	0032991	cellular_component	protein-containing complex
E	0034704	cellular_component	calcium channel complex
E	0035307	biological_process	positive regulation of protein dephosphorylation
E	0035458	biological_process	cellular response to interferon-beta
E	0043209	cellular_component	myelin sheath
E	0043539	molecular_function	protein serine/threonine kinase activator activity
E	0044325	molecular_function	transmembrane transporter binding
E	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
E	0046872	molecular_function	metal ion binding
E	0048306	molecular_function	calcium-dependent protein binding
E	0050848	biological_process	regulation of calcium-mediated signaling
E	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
E	0051592	biological_process	response to calcium ion
E	0055117	biological_process	regulation of cardiac muscle contraction
E	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
E	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
E	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
E	0071346	biological_process	cellular response to type II interferon
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0072542	molecular_function	protein phosphatase activator activity
E	0097225	cellular_component	sperm midpiece
E	0098901	biological_process	regulation of cardiac muscle cell action potential
E	0140056	biological_process	organelle localization by membrane tethering
E	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
E	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
E	1902494	cellular_component	catalytic complex
E	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
E	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
F	0000086	biological_process	G2/M transition of mitotic cell cycle
F	0000922	cellular_component	spindle pole
F	0002027	biological_process	regulation of heart rate
F	0005509	molecular_function	calcium ion binding
F	0005513	biological_process	detection of calcium ion
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005819	cellular_component	spindle
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005876	cellular_component	spindle microtubule
F	0005886	cellular_component	plasma membrane
F	0007186	biological_process	G protein-coupled receptor signaling pathway
F	0008076	cellular_component	voltage-gated potassium channel complex
F	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
F	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
F	0010856	molecular_function	adenylate cyclase activator activity
F	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
F	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
F	0016020	cellular_component	membrane
F	0016240	biological_process	autophagosome membrane docking
F	0019855	molecular_function	calcium channel inhibitor activity
F	0019901	molecular_function	protein kinase binding
F	0021762	biological_process	substantia nigra development
F	0030017	cellular_component	sarcomere
F	0031432	molecular_function	titin binding
F	0031514	cellular_component	motile cilium
F	0031954	biological_process	positive regulation of protein autophosphorylation
F	0031982	cellular_component	vesicle
F	0032465	biological_process	regulation of cytokinesis
F	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
F	0032991	cellular_component	protein-containing complex
F	0034704	cellular_component	calcium channel complex
F	0035307	biological_process	positive regulation of protein dephosphorylation
F	0035458	biological_process	cellular response to interferon-beta
F	0043209	cellular_component	myelin sheath
F	0043539	molecular_function	protein serine/threonine kinase activator activity
F	0044325	molecular_function	transmembrane transporter binding
F	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
F	0046872	molecular_function	metal ion binding
F	0048306	molecular_function	calcium-dependent protein binding
F	0050848	biological_process	regulation of calcium-mediated signaling
F	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
F	0051592	biological_process	response to calcium ion
F	0055117	biological_process	regulation of cardiac muscle contraction
F	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
F	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
F	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
F	0071346	biological_process	cellular response to type II interferon
F	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
F	0072542	molecular_function	protein phosphatase activator activity
F	0097225	cellular_component	sperm midpiece
F	0098901	biological_process	regulation of cardiac muscle cell action potential
F	0140056	biological_process	organelle localization by membrane tethering
F	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
F	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
F	1902494	cellular_component	catalytic complex
F	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
F	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
G	0000086	biological_process	G2/M transition of mitotic cell cycle
G	0000922	cellular_component	spindle pole
G	0002027	biological_process	regulation of heart rate
G	0005509	molecular_function	calcium ion binding
G	0005513	biological_process	detection of calcium ion
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005813	cellular_component	centrosome
G	0005819	cellular_component	spindle
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005876	cellular_component	spindle microtubule
G	0005886	cellular_component	plasma membrane
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0008076	cellular_component	voltage-gated potassium channel complex
G	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
G	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
G	0010856	molecular_function	adenylate cyclase activator activity
G	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
G	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
G	0016020	cellular_component	membrane
G	0016240	biological_process	autophagosome membrane docking
G	0019855	molecular_function	calcium channel inhibitor activity
G	0019901	molecular_function	protein kinase binding
G	0021762	biological_process	substantia nigra development
G	0030017	cellular_component	sarcomere
G	0031432	molecular_function	titin binding
G	0031514	cellular_component	motile cilium
G	0031954	biological_process	positive regulation of protein autophosphorylation
G	0031982	cellular_component	vesicle
G	0032465	biological_process	regulation of cytokinesis
G	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
G	0032991	cellular_component	protein-containing complex
G	0034704	cellular_component	calcium channel complex
G	0035307	biological_process	positive regulation of protein dephosphorylation
G	0035458	biological_process	cellular response to interferon-beta
G	0043209	cellular_component	myelin sheath
G	0043539	molecular_function	protein serine/threonine kinase activator activity
G	0044325	molecular_function	transmembrane transporter binding
G	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
G	0046872	molecular_function	metal ion binding
G	0048306	molecular_function	calcium-dependent protein binding
G	0050848	biological_process	regulation of calcium-mediated signaling
G	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
G	0051592	biological_process	response to calcium ion
G	0055117	biological_process	regulation of cardiac muscle contraction
G	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
G	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
G	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
G	0071346	biological_process	cellular response to type II interferon
G	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
G	0072542	molecular_function	protein phosphatase activator activity
G	0097225	cellular_component	sperm midpiece
G	0098901	biological_process	regulation of cardiac muscle cell action potential
G	0140056	biological_process	organelle localization by membrane tethering
G	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
G	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
G	1902494	cellular_component	catalytic complex
G	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
G	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
H	0000086	biological_process	G2/M transition of mitotic cell cycle
H	0000922	cellular_component	spindle pole
H	0002027	biological_process	regulation of heart rate
H	0005509	molecular_function	calcium ion binding
H	0005513	biological_process	detection of calcium ion
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005813	cellular_component	centrosome
H	0005819	cellular_component	spindle
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0005876	cellular_component	spindle microtubule
H	0005886	cellular_component	plasma membrane
H	0007186	biological_process	G protein-coupled receptor signaling pathway
H	0008076	cellular_component	voltage-gated potassium channel complex
H	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
H	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
H	0010856	molecular_function	adenylate cyclase activator activity
H	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
H	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
H	0016020	cellular_component	membrane
H	0016240	biological_process	autophagosome membrane docking
H	0019855	molecular_function	calcium channel inhibitor activity
H	0019901	molecular_function	protein kinase binding
H	0021762	biological_process	substantia nigra development
H	0030017	cellular_component	sarcomere
H	0031432	molecular_function	titin binding
H	0031514	cellular_component	motile cilium
H	0031954	biological_process	positive regulation of protein autophosphorylation
H	0031982	cellular_component	vesicle
H	0032465	biological_process	regulation of cytokinesis
H	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
H	0032991	cellular_component	protein-containing complex
H	0034704	cellular_component	calcium channel complex
H	0035307	biological_process	positive regulation of protein dephosphorylation
H	0035458	biological_process	cellular response to interferon-beta
H	0043209	cellular_component	myelin sheath
H	0043539	molecular_function	protein serine/threonine kinase activator activity
H	0044325	molecular_function	transmembrane transporter binding
H	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
H	0046872	molecular_function	metal ion binding
H	0048306	molecular_function	calcium-dependent protein binding
H	0050848	biological_process	regulation of calcium-mediated signaling
H	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
H	0051592	biological_process	response to calcium ion
H	0055117	biological_process	regulation of cardiac muscle contraction
H	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
H	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
H	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
H	0071346	biological_process	cellular response to type II interferon
H	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
H	0072542	molecular_function	protein phosphatase activator activity
H	0097225	cellular_component	sperm midpiece
H	0098901	biological_process	regulation of cardiac muscle cell action potential
H	0140056	biological_process	organelle localization by membrane tethering
H	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
H	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
H	1902494	cellular_component	catalytic complex
H	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
H	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
E	ASP20-LEU32
E	ASP56-PHE68
E	ASP93-LEU105
E	ASP129-PHE141

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:25441029, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:4UMO, ECO:0007744|PDB:4V0C, ECO:0007744|PDB:5J03

Chain	Residue	Details
E	ASP20
E	GLU67
H	ASP20
H	ASP22
H	ASP24
H	THR26
H	GLU31
H	ASP56
H	ASP58
H	ASN60
H	THR62
E	ASP22
H	GLU67
G	ASP20
G	ASP22
G	ASP24
G	THR26
G	GLU31
G	ASP56
G	ASP58
G	ASN60
G	THR62
E	ASP24
G	GLU67
F	ASP20
F	ASP22
F	ASP24
F	THR26
F	GLU31
F	ASP56
F	ASP58
F	ASN60
F	THR62
E	THR26
F	GLU67
E	GLU31
E	ASP56
E	ASP58
E	ASN60
E	THR62

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03

Chain	Residue	Details
E	ASP93
E	GLU140
H	ASP93
H	ASP95
H	ASN97
H	TYR99
H	GLU104
H	ASP129
H	ASP131
H	ASP133
H	GLN135
E	ASP95
H	GLU140
G	ASP93
G	ASP95
G	ASN97
G	TYR99
G	GLU104
G	ASP129
G	ASP131
G	ASP133
G	GLN135
E	ASN97
G	GLU140
F	ASP93
F	ASP95
F	ASN97
F	TYR99
F	GLU104
F	ASP129
F	ASP131
F	ASP133
F	GLN135
E	TYR99
F	GLU140
E	GLU104
E	ASP129
E	ASP131
E	ASP133
E	GLN135

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
E	LYS21
H	LYS21
G	LYS21
F	LYS21

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29

Chain	Residue	Details
E	THR44
H	THR44
G	THR44
F	THR44
C	HIS270-ASN290
C	ASP342-ALA350
B	HIS270-ASN290
B	ASP342-ALA350

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
E	SER81
H	SER81
G	SER81
F	SER81

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
E	LYS94
H	LYS94
G	LYS94
F	LYS94

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
E	TYR99
H	TYR99
G	TYR99
F	TYR99

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
E	SER101
H	SER101
G	SER101
F	SER101

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
E	THR110
H	THR110
G	THR110
F	THR110

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315

Chain	Residue	Details
E	LYS115
H	LYS115
G	LYS115
F	LYS115

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
E	TYR138
H	TYR138
G	TYR138
F	TYR138

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157

Chain	Residue	Details
E	LYS21
H	LYS21
G	LYS21
F	LYS21

---