8E78

Cryo-EM structure of human ME3 in the presence of citrate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004470	molecular_function	malic enzyme activity
A	0004473	molecular_function	malate dehydrogenase (decarboxylating) (NADP+) activity
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006108	biological_process	malate metabolic process
A	0008948	molecular_function	oxaloacetate decarboxylase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0042866	biological_process	pyruvate biosynthetic process
A	0046872	molecular_function	metal ion binding
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
A	0070401	molecular_function	NADP+ binding
B	0004470	molecular_function	malic enzyme activity
B	0004473	molecular_function	malate dehydrogenase (decarboxylating) (NADP+) activity
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006108	biological_process	malate metabolic process
B	0008948	molecular_function	oxaloacetate decarboxylase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0042866	biological_process	pyruvate biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
B	0070401	molecular_function	NADP+ binding
C	0004470	molecular_function	malic enzyme activity
C	0004473	molecular_function	malate dehydrogenase (decarboxylating) (NADP+) activity
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0006108	biological_process	malate metabolic process
C	0008948	molecular_function	oxaloacetate decarboxylase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0042866	biological_process	pyruvate biosynthetic process
C	0046872	molecular_function	metal ion binding
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
C	0070401	molecular_function	NADP+ binding
D	0004470	molecular_function	malic enzyme activity
D	0004473	molecular_function	malate dehydrogenase (decarboxylating) (NADP+) activity
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0006108	biological_process	malate metabolic process
D	0008948	molecular_function	oxaloacetate decarboxylase activity
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0042866	biological_process	pyruvate biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
D	0070401	molecular_function	NADP+ binding

Functional Information from PROSITE/UniProt

site_id	PS00331
Number of Residues	17
Details	MALIC_ENZYMES Malic enzymes signature. FnDDiqGTAsVaVAGIL

Chain	Residue	Details
A	PHE253-LEU269

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000250

Chain	Residue	Details
A	TYR89
B	TYR89
C	TYR89
D	TYR89

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000250

Chain	Residue	Details
A	LYS160
B	LYS160
C	LYS160
D	LYS160

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site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ARG142
B	ASN395
C	ARG142
C	GLU232
C	ASP233
C	ASP256
C	ASN395
D	ARG142
D	GLU232
D	ASP233
D	ASP256
A	GLU232
D	ASN395
A	ASP233
A	ASP256
A	ASN395
B	ARG142
B	GLU232
B	ASP233
B	ASP256

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site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Important for activity => ECO:0000250

Chain	Residue	Details
A	ASP256
B	ASP256
C	ASP256
D	ASP256

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site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P06801

Chain	Residue	Details
A	SER323
B	SER323
C	SER323
D	SER323

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