8E5T

Yeast co-transcriptional Noc1-Noc2 RNP assembly checkpoint intermediate

Functional Information from GO Data

Chain	GOid	namespace	contents
5	0000027	biological_process	ribosomal large subunit assembly
5	0003729	molecular_function	mRNA binding
5	0005515	molecular_function	protein binding
5	0005634	cellular_component	nucleus
5	0005730	cellular_component	nucleolus
5	0030687	cellular_component	preribosome, large subunit precursor
5	0030690	cellular_component	Noc1p-Noc2p complex
5	0042254	biological_process	ribosome biogenesis
5	0042273	biological_process	ribosomal large subunit biogenesis
5	0042802	molecular_function	identical protein binding
5	0043231	cellular_component	intracellular membrane-bounded organelle
6	0003729	molecular_function	mRNA binding
6	0005515	molecular_function	protein binding
6	0005634	cellular_component	nucleus
6	0005654	cellular_component	nucleoplasm
6	0005730	cellular_component	nucleolus
6	0005739	cellular_component	mitochondrion
6	0030687	cellular_component	preribosome, large subunit precursor
6	0030690	cellular_component	Noc1p-Noc2p complex
6	0030691	cellular_component	Noc2p-Noc3p complex
6	0042254	biological_process	ribosome biogenesis
6	0042273	biological_process	ribosomal large subunit biogenesis
b	0000027	biological_process	ribosomal large subunit assembly
b	0000464	biological_process	endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
b	0000465	biological_process	exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
b	0003723	molecular_function	RNA binding
b	0005515	molecular_function	protein binding
b	0005634	cellular_component	nucleus
b	0005730	cellular_component	nucleolus
b	0006364	biological_process	rRNA processing
b	0008097	molecular_function	5S rRNA binding
b	0019843	molecular_function	rRNA binding
b	0030687	cellular_component	preribosome, large subunit precursor
b	0042134	molecular_function	rRNA primary transcript binding
b	0042254	biological_process	ribosome biogenesis
C	0002181	biological_process	cytoplasmic translation
C	0003723	molecular_function	RNA binding
C	0003735	molecular_function	structural constituent of ribosome
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005840	cellular_component	ribosome
C	0006412	biological_process	translation
C	0022625	cellular_component	cytosolic large ribosomal subunit
C	1990904	cellular_component	ribonucleoprotein complex
D	0000460	biological_process	maturation of 5.8S rRNA
D	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
D	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
D	0000470	biological_process	maturation of LSU-rRNA
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005730	cellular_component	nucleolus
D	0030687	cellular_component	preribosome, large subunit precursor
D	0042273	biological_process	ribosomal large subunit biogenesis
e	0002181	biological_process	cytoplasmic translation
e	0003735	molecular_function	structural constituent of ribosome
e	0005737	cellular_component	cytoplasm
e	0005829	cellular_component	cytosol
e	0005840	cellular_component	ribosome
e	0006412	biological_process	translation
e	0022625	cellular_component	cytosolic large ribosomal subunit
e	1990904	cellular_component	ribonucleoprotein complex
E	0000027	biological_process	ribosomal large subunit assembly
E	0002181	biological_process	cytoplasmic translation
E	0003723	molecular_function	RNA binding
E	0003735	molecular_function	structural constituent of ribosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005840	cellular_component	ribosome
E	0006412	biological_process	translation
E	0022625	cellular_component	cytosolic large ribosomal subunit
E	1990904	cellular_component	ribonucleoprotein complex
f	0000027	biological_process	ribosomal large subunit assembly
f	0002181	biological_process	cytoplasmic translation
f	0003735	molecular_function	structural constituent of ribosome
f	0005737	cellular_component	cytoplasm
f	0005829	cellular_component	cytosol
f	0005840	cellular_component	ribosome
f	0006364	biological_process	rRNA processing
f	0006412	biological_process	translation
f	0022625	cellular_component	cytosolic large ribosomal subunit
f	0042273	biological_process	ribosomal large subunit biogenesis
f	1990904	cellular_component	ribonucleoprotein complex
F	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
F	0000470	biological_process	maturation of LSU-rRNA
F	0002181	biological_process	cytoplasmic translation
F	0003723	molecular_function	RNA binding
F	0003735	molecular_function	structural constituent of ribosome
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005840	cellular_component	ribosome
F	0022625	cellular_component	cytosolic large ribosomal subunit
F	0042273	biological_process	ribosomal large subunit biogenesis
F	1990904	cellular_component	ribonucleoprotein complex
G	0000470	biological_process	maturation of LSU-rRNA
G	0002181	biological_process	cytoplasmic translation
G	0003723	molecular_function	RNA binding
G	0003735	molecular_function	structural constituent of ribosome
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005840	cellular_component	ribosome
G	0022625	cellular_component	cytosolic large ribosomal subunit
G	0042254	biological_process	ribosome biogenesis
G	1990904	cellular_component	ribonucleoprotein complex
i	0002181	biological_process	cytoplasmic translation
i	0003723	molecular_function	RNA binding
i	0003735	molecular_function	structural constituent of ribosome
i	0005737	cellular_component	cytoplasm
i	0005829	cellular_component	cytosol
i	0005840	cellular_component	ribosome
i	0006412	biological_process	translation
i	0022625	cellular_component	cytosolic large ribosomal subunit
i	1990904	cellular_component	ribonucleoprotein complex
K	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
K	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
K	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
K	0000502	cellular_component	proteasome complex
K	0003723	molecular_function	RNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005730	cellular_component	nucleolus
K	0019843	molecular_function	rRNA binding
K	0030163	biological_process	protein catabolic process
K	0030674	molecular_function	protein-macromolecule adaptor activity
K	0030687	cellular_component	preribosome, large subunit precursor
K	0042254	biological_process	ribosome biogenesis
K	0042802	molecular_function	identical protein binding
K	0070180	molecular_function	large ribosomal subunit rRNA binding
K	0070628	molecular_function	proteasome binding
K	1990904	cellular_component	ribonucleoprotein complex
L	0002181	biological_process	cytoplasmic translation
L	0003723	molecular_function	RNA binding
L	0003735	molecular_function	structural constituent of ribosome
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005840	cellular_component	ribosome
L	0006412	biological_process	translation
L	0022625	cellular_component	cytosolic large ribosomal subunit
L	1990904	cellular_component	ribonucleoprotein complex
m	0000280	biological_process	nuclear division
m	0003729	molecular_function	mRNA binding
m	0005515	molecular_function	protein binding
m	0005634	cellular_component	nucleus
m	0005730	cellular_component	nucleolus
m	0006364	biological_process	rRNA processing
m	0030687	cellular_component	preribosome, large subunit precursor
m	0034399	cellular_component	nuclear periphery
m	0042254	biological_process	ribosome biogenesis
m	0042273	biological_process	ribosomal large subunit biogenesis
m	0042802	molecular_function	identical protein binding
M	0000027	biological_process	ribosomal large subunit assembly
M	0000470	biological_process	maturation of LSU-rRNA
M	0002181	biological_process	cytoplasmic translation
M	0003723	molecular_function	RNA binding
M	0003735	molecular_function	structural constituent of ribosome
M	0005730	cellular_component	nucleolus
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005840	cellular_component	ribosome
M	0006412	biological_process	translation
M	0016236	biological_process	macroautophagy
M	0022625	cellular_component	cytosolic large ribosomal subunit
M	0032991	cellular_component	protein-containing complex
M	0042273	biological_process	ribosomal large subunit biogenesis
M	0043229	cellular_component	intracellular organelle
M	1990904	cellular_component	ribonucleoprotein complex
n	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
n	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
n	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
n	0003723	molecular_function	RNA binding
n	0003729	molecular_function	mRNA binding
n	0005515	molecular_function	protein binding
n	0005634	cellular_component	nucleus
n	0005654	cellular_component	nucleoplasm
n	0005730	cellular_component	nucleolus
n	0006364	biological_process	rRNA processing
n	0030687	cellular_component	preribosome, large subunit precursor
n	0042254	biological_process	ribosome biogenesis
n	0042273	biological_process	ribosomal large subunit biogenesis
n	0043021	molecular_function	ribonucleoprotein complex binding
n	0070180	molecular_function	large ribosomal subunit rRNA binding
n	0070545	cellular_component	PeBoW complex
N	0002181	biological_process	cytoplasmic translation
N	0003723	molecular_function	RNA binding
N	0003735	molecular_function	structural constituent of ribosome
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005840	cellular_component	ribosome
N	0006412	biological_process	translation
N	0022625	cellular_component	cytosolic large ribosomal subunit
N	0022626	cellular_component	cytosolic ribosome
N	1990904	cellular_component	ribonucleoprotein complex
o	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
o	0003674	molecular_function	molecular_function
o	0003676	molecular_function	nucleic acid binding
o	0003723	molecular_function	RNA binding
o	0005515	molecular_function	protein binding
o	0005634	cellular_component	nucleus
o	0005730	cellular_component	nucleolus
o	0005737	cellular_component	cytoplasm
o	0006364	biological_process	rRNA processing
o	0019843	molecular_function	rRNA binding
o	0030687	cellular_component	preribosome, large subunit precursor
o	0042254	biological_process	ribosome biogenesis
o	0042273	biological_process	ribosomal large subunit biogenesis
o	1990904	cellular_component	ribonucleoprotein complex
O	0000470	biological_process	maturation of LSU-rRNA
O	0002181	biological_process	cytoplasmic translation
O	0003723	molecular_function	RNA binding
O	0003729	molecular_function	mRNA binding
O	0003735	molecular_function	structural constituent of ribosome
O	0005730	cellular_component	nucleolus
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005840	cellular_component	ribosome
O	0006412	biological_process	translation
O	0015934	cellular_component	large ribosomal subunit
O	0017148	biological_process	negative regulation of translation
O	0022625	cellular_component	cytosolic large ribosomal subunit
O	1990904	cellular_component	ribonucleoprotein complex
p	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
p	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
p	0003676	molecular_function	nucleic acid binding
p	0003723	molecular_function	RNA binding
p	0003724	molecular_function	RNA helicase activity
p	0004386	molecular_function	helicase activity
p	0005515	molecular_function	protein binding
p	0005524	molecular_function	ATP binding
p	0005634	cellular_component	nucleus
p	0005635	cellular_component	nuclear envelope
p	0005730	cellular_component	nucleolus
p	0006364	biological_process	rRNA processing
p	0006396	biological_process	RNA processing
p	0008186	molecular_function	ATP-dependent activity, acting on RNA
p	0016787	molecular_function	hydrolase activity
p	0016887	molecular_function	ATP hydrolysis activity
p	0022613	biological_process	ribonucleoprotein complex biogenesis
p	0030490	biological_process	maturation of SSU-rRNA
p	0030686	cellular_component	90S preribosome
p	0030687	cellular_component	preribosome, large subunit precursor
p	0032040	cellular_component	small-subunit processome
p	0042254	biological_process	ribosome biogenesis
p	0042273	biological_process	ribosomal large subunit biogenesis
p	0042274	biological_process	ribosomal small subunit biogenesis
p	0042802	molecular_function	identical protein binding
p	1990417	biological_process	snoRNA release from pre-rRNA
Q	0002181	biological_process	cytoplasmic translation
Q	0003723	molecular_function	RNA binding
Q	0003735	molecular_function	structural constituent of ribosome
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005840	cellular_component	ribosome
Q	0006412	biological_process	translation
Q	0022625	cellular_component	cytosolic large ribosomal subunit
Q	0043232	cellular_component	intracellular non-membrane-bounded organelle
Q	1990904	cellular_component	ribonucleoprotein complex
s	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
s	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
s	0003674	molecular_function	molecular_function
s	0003677	molecular_function	DNA binding
s	0005515	molecular_function	protein binding
s	0005634	cellular_component	nucleus
s	0005654	cellular_component	nucleoplasm
s	0005730	cellular_component	nucleolus
s	0006364	biological_process	rRNA processing
s	0030687	cellular_component	preribosome, large subunit precursor
s	0042254	biological_process	ribosome biogenesis
s	0042273	biological_process	ribosomal large subunit biogenesis
s	0043021	molecular_function	ribonucleoprotein complex binding
s	0070180	molecular_function	large ribosomal subunit rRNA binding
s	0070545	cellular_component	PeBoW complex
S	0002181	biological_process	cytoplasmic translation
S	0003735	molecular_function	structural constituent of ribosome
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0005840	cellular_component	ribosome
S	0006412	biological_process	translation
S	0022625	cellular_component	cytosolic large ribosomal subunit
S	1990904	cellular_component	ribonucleoprotein complex
t	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
t	0000465	biological_process	exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
t	0003723	molecular_function	RNA binding
t	0003729	molecular_function	mRNA binding
t	0003735	molecular_function	structural constituent of ribosome
t	0005515	molecular_function	protein binding
t	0005634	cellular_component	nucleus
t	0005730	cellular_component	nucleolus
t	0022625	cellular_component	cytosolic large ribosomal subunit
t	0030687	cellular_component	preribosome, large subunit precursor
t	0042134	molecular_function	rRNA primary transcript binding
t	0042254	biological_process	ribosome biogenesis
t	0042273	biological_process	ribosomal large subunit biogenesis
z	0003674	molecular_function	molecular_function
z	0005515	molecular_function	protein binding
z	0005634	cellular_component	nucleus
z	0005730	cellular_component	nucleolus
z	0006364	biological_process	rRNA processing
z	0030687	cellular_component	preribosome, large subunit precursor
z	0030688	cellular_component	preribosome, small subunit precursor

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. IIDEADRiL

Chain	Residue	Details
p	ILE194-LEU202

---

site_id	PS00580
Number of Residues	21
Details	RIBOSOMAL_L32E Ribosomal protein L32e signature. FkRhhsdRyhRVaen..WRKqkG

Chain	Residue	Details
e	PHE17-GLY37

---

site_id	PS00634
Number of Residues	33
Details	RIBOSOMAL_L30 Ribosomal protein L30 signature. VLqlLrLtRinSgTfvkvtkatleLLklIepYV

Chain	Residue	Details
F	VAL102-VAL134
t	ILE162-VAL194

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LATGsdDgTVRVWEI

Chain	Residue	Details
s	LEU452-ILE466

---

site_id	PS00783
Number of Residues	24
Details	RIBOSOMAL_L13 Ribosomal protein L13 signature. LRGMVShktarGKaalerLkVFeG

Chain	Residue	Details
O	LEU84-GLY107

---

site_id	PS00939
Number of Residues	27
Details	RIBOSOMAL_L1E Ribosomal protein L1e signature. NhneKryATaSAIaATAvaslvlaRGH

Chain	Residue	Details
C	ASN114-HIS140

---

site_id	PS01082
Number of Residues	18
Details	RIBOSOMAL_L7AE Ribosomal protein L7Ae signature. CkkmgVPYaiVkGKarLG

Chain	Residue	Details
G	CYS170-GLY187

---

site_id	PS01104
Number of Residues	15
Details	RIBOSOMAL_L13E Ribosomal protein L13e signature. RaGRGFTLaEVkaaG

Chain	Residue	Details
L	ARG70-GLY84

---

site_id	PS01105
Number of Residues	22
Details	RIBOSOMAL_L35AE Ribosomal protein L35Ae signature. GKVtRtHGnsGvVrAtFrnnLP

Chain	Residue	Details
f	GLY69-PRO90

---

site_id	PS01106
Number of Residues	18
Details	RIBOSOMAL_L18E Ribosomal protein L18e signature. KaLflSKinRPpVSVsRI

Chain	Residue	Details
Q	LYS50-ILE67

---

site_id	PS01170
Number of Residues	19
Details	RIBOSOMAL_L6E Ribosomal protein L6e signature. NgvPLRRvnarYVIATSt.K

Chain	Residue	Details
E	ASN72-LYS90

---

site_id	PS01190
Number of Residues	11
Details	RIBOSOMAL_L36E Ribosomal protein L36e signature. PYERRlIDLIR

Chain	Residue	Details
i	PRO52-ARG62

---

site_id	PS01194
Number of Residues	24
Details	RIBOSOMAL_L15E Ribosomal protein L15e signature. DKARrLGYkAkqGFVIYRvRVrRG

Chain	Residue	Details
N	ASP46-GLY69

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956

Chain	Residue	Details
6	SER70
m	SER177
m	SER183

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
6	SER149
s	SER76
S	LYS131
S	LYS149

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
6	SER160
6	SER698
6	SER708

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950

Chain	Residue	Details
6	SER166

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
s	LYS127
5	SER874
5	SER1024

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
5	TYR708

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
5	SER710
5	SER878

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950

Chain	Residue	Details
5	SER977
5	SER978

---