8DW7
DNA glycosylase MutY variant N146S in complex with DNA containing the transition state analog 1N paired with d(8-oxo-G)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000701 | molecular_function | purine-specific mismatch base pair DNA N-glycosylase activity |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0006281 | biological_process | DNA repair |
| A | 0006284 | biological_process | base-excision repair |
| A | 0006298 | biological_process | mismatch repair |
| A | 0006950 | biological_process | response to stress |
| A | 0006974 | biological_process | DNA damage response |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0019104 | molecular_function | DNA N-glycosylase activity |
| A | 0032357 | molecular_function | oxidized purine DNA binding |
| A | 0034039 | molecular_function | 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity |
| A | 0035485 | molecular_function | adenine/guanine mispair binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051536 | molecular_function | iron-sulfur cluster binding |
| A | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| D | 0000701 | molecular_function | purine-specific mismatch base pair DNA N-glycosylase activity |
| D | 0003677 | molecular_function | DNA binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0006281 | biological_process | DNA repair |
| D | 0006284 | biological_process | base-excision repair |
| D | 0006298 | biological_process | mismatch repair |
| D | 0006950 | biological_process | response to stress |
| D | 0006974 | biological_process | DNA damage response |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| D | 0019104 | molecular_function | DNA N-glycosylase activity |
| D | 0032357 | molecular_function | oxidized purine DNA binding |
| D | 0034039 | molecular_function | 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity |
| D | 0035485 | molecular_function | adenine/guanine mispair binding |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051536 | molecular_function | iron-sulfur cluster binding |
| D | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:19841264 |
| Chain | Residue | Details |
| A | GLU43 | |
| D | GLU43 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:19841264 |
| Chain | Residue | Details |
| A | TRP30 | |
| A | TYR126 | |
| A | GLU188 | |
| D | TRP30 | |
| D | TYR126 | |
| D | GLU188 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14961129, ECO:0000269|PubMed:19841264 |
| Chain | Residue | Details |
| A | GLN48 | |
| A | LEU86 | |
| A | SER308 | |
| D | GLN48 | |
| D | LEU86 | |
| D | SER308 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14961129, ECO:0000269|PubMed:19841264, ECO:0000269|PubMed:25995449, ECO:0000269|Ref.3, ECO:0000269|Ref.5, ECO:0007744|PDB:1RRQ, ECO:0007744|PDB:1RRS, ECO:0007744|PDB:1VRL |
| Chain | Residue | Details |
| A | CYS198 | |
| A | CYS205 | |
| A | CYS208 | |
| A | CYS214 | |
| D | CYS198 | |
| D | CYS205 | |
| D | CYS208 | |
| D | CYS214 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | SITE: Transition state stabilizer => ECO:0000305|PubMed:19841264, ECO:0000305|PubMed:25995449 |
| Chain | Residue | Details |
| A | ASP144 | |
| D | ASP144 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 807 |
| Chain | Residue | Details |
| A | GLU43 | activator, proton acceptor, proton donor |
| A | TYR126 | electrostatic stabiliser |
| A | ASP144 | covalently attached, nucleofuge, nucleophile |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 807 |
| Chain | Residue | Details |
| D | GLU43 | activator, proton acceptor, proton donor |
| D | TYR126 | electrostatic stabiliser |
| D | ASP144 | covalently attached, nucleofuge, nucleophile |
