8CX2

Cryo-EM structure of human APOBEC3G/HIV-1 Vif/CBFbeta/ELOB/ELOC dimeric complex in State 2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000932	cellular_component	P-body
A	0002230	biological_process	positive regulation of defense response to virus by host
A	0003723	molecular_function	RNA binding
A	0003824	molecular_function	catalytic activity
A	0004126	molecular_function	cytidine deaminase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008270	molecular_function	zinc ion binding
A	0008829	molecular_function	dCTP deaminase activity
A	0009972	biological_process	cytidine deamination
A	0010526	biological_process	retrotransposon silencing
A	0016553	biological_process	base conversion or substitution editing
A	0016554	biological_process	cytidine to uridine editing
A	0016787	molecular_function	hydrolase activity
A	0030895	cellular_component	apolipoprotein B mRNA editing enzyme complex
A	0042802	molecular_function	identical protein binding
A	0045071	biological_process	negative regulation of viral genome replication
A	0045087	biological_process	innate immune response
A	0045869	biological_process	negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
A	0046872	molecular_function	metal ion binding
A	0047844	molecular_function	obsolete deoxycytidine deaminase activity
A	0048525	biological_process	negative regulation of viral process
A	0051607	biological_process	defense response to virus
A	0070383	biological_process	DNA cytosine deamination
A	0080111	biological_process	DNA demethylation
A	1990904	cellular_component	ribonucleoprotein complex
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0008289	molecular_function	lipid binding
B	0016020	cellular_component	membrane
B	0019058	biological_process	viral life cycle
B	0019079	biological_process	viral genome replication
B	0020002	cellular_component	host cell plasma membrane
B	0030430	cellular_component	host cell cytoplasm
B	0042802	molecular_function	identical protein binding
B	0044423	cellular_component	virion component
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000209	biological_process	protein polyubiquitination
C	0001503	biological_process	ossification
C	0001649	biological_process	osteoblast differentiation
C	0003677	molecular_function	DNA binding
C	0003713	molecular_function	transcription coactivator activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006366	biological_process	transcription by RNA polymerase II
C	0016020	cellular_component	membrane
C	0016513	cellular_component	core-binding factor complex
C	0030098	biological_process	lymphocyte differentiation
C	0030099	biological_process	myeloid cell differentiation
C	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
C	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
C	0043565	molecular_function	sequence-specific DNA binding
C	0045944	biological_process	positive regulation of transcription by RNA polymerase II
C	0048469	biological_process	cell maturation
C	0060216	biological_process	definitive hemopoiesis
D	0001222	molecular_function	transcription corepressor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005829	cellular_component	cytosol
D	0006367	biological_process	transcription initiation at RNA polymerase II promoter
D	0006368	biological_process	transcription elongation by RNA polymerase II
D	0016567	biological_process	protein ubiquitination
D	0030891	cellular_component	VCB complex
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0065003	biological_process	protein-containing complex assembly
D	0070449	cellular_component	elongin complex
D	0140958	biological_process	target-directed miRNA degradation
E	0001222	molecular_function	transcription corepressor binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005829	cellular_component	cytosol
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0006367	biological_process	transcription initiation at RNA polymerase II promoter
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0070449	cellular_component	elongin complex
E	0140958	biological_process	target-directed miRNA degradation
F	0000932	cellular_component	P-body
F	0002230	biological_process	positive regulation of defense response to virus by host
F	0003723	molecular_function	RNA binding
F	0003824	molecular_function	catalytic activity
F	0004126	molecular_function	cytidine deaminase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0008270	molecular_function	zinc ion binding
F	0008829	molecular_function	dCTP deaminase activity
F	0009972	biological_process	cytidine deamination
F	0010526	biological_process	retrotransposon silencing
F	0016553	biological_process	base conversion or substitution editing
F	0016554	biological_process	cytidine to uridine editing
F	0016787	molecular_function	hydrolase activity
F	0030895	cellular_component	apolipoprotein B mRNA editing enzyme complex
F	0042802	molecular_function	identical protein binding
F	0045071	biological_process	negative regulation of viral genome replication
F	0045087	biological_process	innate immune response
F	0045869	biological_process	negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
F	0046872	molecular_function	metal ion binding
F	0047844	molecular_function	obsolete deoxycytidine deaminase activity
F	0048525	biological_process	negative regulation of viral process
F	0051607	biological_process	defense response to virus
F	0070383	biological_process	DNA cytosine deamination
F	0080111	biological_process	DNA demethylation
F	1990904	cellular_component	ribonucleoprotein complex
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0008289	molecular_function	lipid binding
G	0016020	cellular_component	membrane
G	0019058	biological_process	viral life cycle
G	0019079	biological_process	viral genome replication
G	0020002	cellular_component	host cell plasma membrane
G	0030430	cellular_component	host cell cytoplasm
G	0042802	molecular_function	identical protein binding
G	0044423	cellular_component	virion component
H	0000122	biological_process	negative regulation of transcription by RNA polymerase II
H	0000209	biological_process	protein polyubiquitination
H	0001503	biological_process	ossification
H	0001649	biological_process	osteoblast differentiation
H	0003677	molecular_function	DNA binding
H	0003713	molecular_function	transcription coactivator activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0006357	biological_process	regulation of transcription by RNA polymerase II
H	0006366	biological_process	transcription by RNA polymerase II
H	0016020	cellular_component	membrane
H	0016513	cellular_component	core-binding factor complex
H	0030098	biological_process	lymphocyte differentiation
H	0030099	biological_process	myeloid cell differentiation
H	0043371	biological_process	negative regulation of CD4-positive, alpha-beta T cell differentiation
H	0043378	biological_process	positive regulation of CD8-positive, alpha-beta T cell differentiation
H	0043565	molecular_function	sequence-specific DNA binding
H	0045944	biological_process	positive regulation of transcription by RNA polymerase II
H	0048469	biological_process	cell maturation
H	0060216	biological_process	definitive hemopoiesis
I	0001222	molecular_function	transcription corepressor binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005829	cellular_component	cytosol
I	0006367	biological_process	transcription initiation at RNA polymerase II promoter
I	0006368	biological_process	transcription elongation by RNA polymerase II
I	0016567	biological_process	protein ubiquitination
I	0030891	cellular_component	VCB complex
I	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
I	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
I	0031625	molecular_function	ubiquitin protein ligase binding
I	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
I	0065003	biological_process	protein-containing complex assembly
I	0070449	cellular_component	elongin complex
I	0140958	biological_process	target-directed miRNA degradation
J	0001222	molecular_function	transcription corepressor binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005829	cellular_component	cytosol
J	0006357	biological_process	regulation of transcription by RNA polymerase II
J	0006367	biological_process	transcription initiation at RNA polymerase II promoter
J	0006511	biological_process	ubiquitin-dependent protein catabolic process
J	0016567	biological_process	protein ubiquitination
J	0030674	molecular_function	protein-macromolecule adaptor activity
J	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
J	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
J	0070449	cellular_component	elongin complex
J	0140958	biological_process	target-directed miRNA degradation

Functional Information from PROSITE/UniProt

site_id	PS00903
Number of Residues	39
Details	CYT_DCMP_DEAMINASES_1 Cytidine and deoxycytidylate deaminases zinc-binding region signature. HAElcFLdvipfwkldldqdyrvtcftsws..........PCfs......CaqeM

Chain	Residue	Details
A	HIS257-MET295

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Cleavage in virion (by viral protease) => ECO:0000255|HAMAP-Rule:MF_04081

Chain	Residue	Details
B	LEU150
G	LEU150

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site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphothreonine; by host MAP4K1 => ECO:0000255|HAMAP-Rule:MF_04081, ECO:0000269|PubMed:9792705

Chain	Residue	Details
B	THR96
G	THR96
A	CYS100
F	HIS65
F	CYS97
F	CYS100

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site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine; by host => ECO:0000255|HAMAP-Rule:MF_04081, ECO:0000269|PubMed:8626571

Chain	Residue	Details
B	SER144
G	SER144
I	SER108
I	SER111
F	CYS288
F	CYS291

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site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by host => ECO:0000255|HAMAP-Rule:MF_04081, ECO:0000269|PubMed:8626571

Chain	Residue	Details
B	THR155
B	THR188
G	THR155
G	THR188

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site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine; by host MAP4K1 => ECO:0000255|HAMAP-Rule:MF_04081, ECO:0000269|PubMed:9792705

Chain	Residue	Details
B	SER165
G	SER165

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site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine; by PKA and CAMK2 => ECO:0000269|PubMed:21659520

Chain	Residue	Details
A	THR218
F	THR218

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