8CV2

Human excitatory amino acid transporter 3 (EAAT3) in an outward facing sodium-bound state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001662	biological_process	behavioral fear response
A	0001975	biological_process	response to amphetamine
A	0002027	biological_process	regulation of heart rate
A	0005253	molecular_function	monoatomic anion channel activity
A	0005313	molecular_function	L-glutamate transmembrane transporter activity
A	0005314	molecular_function	high-affinity L-glutamate transmembrane transporter activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005768	cellular_component	endosome
A	0005886	cellular_component	plasma membrane
A	0006749	biological_process	glutathione metabolic process
A	0006750	biological_process	glutathione biosynthetic process
A	0006801	biological_process	superoxide metabolic process
A	0006811	biological_process	monoatomic ion transport
A	0006836	biological_process	neurotransmitter transport
A	0006865	biological_process	amino acid transport
A	0006882	biological_process	intracellular zinc ion homeostasis
A	0007212	biological_process	G protein-coupled dopamine receptor signaling pathway
A	0007215	biological_process	glutamate receptor signaling pathway
A	0007268	biological_process	chemical synaptic transmission
A	0007420	biological_process	brain development
A	0007611	biological_process	learning or memory
A	0007613	biological_process	memory
A	0007625	biological_process	grooming behavior
A	0007626	biological_process	locomotory behavior
A	0009410	biological_process	response to xenobiotic stimulus
A	0009986	cellular_component	cell surface
A	0010460	biological_process	positive regulation of heart rate
A	0010467	biological_process	gene expression
A	0010842	biological_process	retina layer formation
A	0015108	molecular_function	chloride transmembrane transporter activity
A	0015183	molecular_function	L-aspartate transmembrane transporter activity
A	0015293	molecular_function	symporter activity
A	0015501	molecular_function	glutamate:sodium symporter activity
A	0015813	biological_process	L-glutamate transmembrane transport
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0019221	biological_process	cytokine-mediated signaling pathway
A	0022008	biological_process	neurogenesis
A	0030424	cellular_component	axon
A	0030425	cellular_component	dendrite
A	0030534	biological_process	adult behavior
A	0031901	cellular_component	early endosome membrane
A	0031902	cellular_component	late endosome membrane
A	0031982	cellular_component	vesicle
A	0032279	cellular_component	asymmetric synapse
A	0033229	molecular_function	cysteine transmembrane transporter activity
A	0034599	biological_process	cellular response to oxidative stress
A	0035633	biological_process	maintenance of blood-brain barrier
A	0036293	biological_process	response to decreased oxygen levels
A	0042417	biological_process	dopamine metabolic process
A	0042802	molecular_function	identical protein binding
A	0042883	biological_process	cysteine transport
A	0043005	cellular_component	neuron projection
A	0043025	cellular_component	neuronal cell body
A	0043083	cellular_component	synaptic cleft
A	0043197	cellular_component	dendritic spine
A	0043198	cellular_component	dendritic shaft
A	0043204	cellular_component	perikaryon
A	0043278	biological_process	response to morphine
A	0043524	biological_process	negative regulation of neuron apoptotic process
A	0043679	cellular_component	axon terminus
A	0045121	cellular_component	membrane raft
A	0045184	biological_process	establishment of protein localization
A	0045202	cellular_component	synapse
A	0046872	molecular_function	metal ion binding
A	0048514	biological_process	blood vessel morphogenesis
A	0048678	biological_process	response to axon injury
A	0050808	biological_process	synapse organization
A	0051402	biological_process	neuron apoptotic process
A	0051938	biological_process	L-glutamate import
A	0055037	cellular_component	recycling endosome
A	0055038	cellular_component	recycling endosome membrane
A	0060013	biological_process	righting reflex
A	0060041	biological_process	retina development in camera-type eye
A	0060047	biological_process	heart contraction
A	0060291	biological_process	long-term synaptic potentiation
A	0061744	biological_process	motor behavior
A	0070062	cellular_component	extracellular exosome
A	0070633	biological_process	transepithelial transport
A	0070777	biological_process	D-aspartate transmembrane transport
A	0070778	biological_process	L-aspartate transmembrane transport
A	0070779	biological_process	D-aspartate import across plasma membrane
A	0071242	biological_process	cellular response to ammonium ion
A	0071288	biological_process	cellular response to mercury ion
A	0071314	biological_process	cellular response to cocaine
A	0071407	biological_process	obsolete cellular response to organic cyclic compound
A	0071577	biological_process	zinc ion transmembrane transport
A	0071944	cellular_component	cell periphery
A	0072347	biological_process	response to anesthetic
A	0090313	biological_process	regulation of protein targeting to membrane
A	0090461	biological_process	intracellular glutamate homeostasis
A	0097049	biological_process	motor neuron apoptotic process
A	0097386	cellular_component	glial cell projection
A	0097440	cellular_component	apical dendrite
A	0098657	biological_process	import into cell
A	0098685	cellular_component	Schaffer collateral - CA1 synapse
A	0098712	biological_process	L-glutamate import across plasma membrane
A	0098793	cellular_component	presynapse
A	0098877	biological_process	neurotransmitter receptor transport to plasma membrane
A	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
A	0099544	cellular_component	perisynaptic space
A	0140009	biological_process	L-aspartate import across plasma membrane
A	0140010	molecular_function	D-aspartate transmembrane transporter activity
A	0150002	cellular_component	distal dendrite
A	0150104	biological_process	transport across blood-brain barrier
A	1902476	biological_process	chloride transmembrane transport
A	1903712	biological_process	cysteine transmembrane transport
A	1903926	biological_process	cellular response to bisphenol A
A	1990635	cellular_component	proximal dendrite
A	1990708	biological_process	conditioned place preference
B	0001662	biological_process	behavioral fear response
B	0001975	biological_process	response to amphetamine
B	0002027	biological_process	regulation of heart rate
B	0005253	molecular_function	monoatomic anion channel activity
B	0005313	molecular_function	L-glutamate transmembrane transporter activity
B	0005314	molecular_function	high-affinity L-glutamate transmembrane transporter activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005768	cellular_component	endosome
B	0005886	cellular_component	plasma membrane
B	0006749	biological_process	glutathione metabolic process
B	0006750	biological_process	glutathione biosynthetic process
B	0006801	biological_process	superoxide metabolic process
B	0006811	biological_process	monoatomic ion transport
B	0006836	biological_process	neurotransmitter transport
B	0006865	biological_process	amino acid transport
B	0006882	biological_process	intracellular zinc ion homeostasis
B	0007212	biological_process	G protein-coupled dopamine receptor signaling pathway
B	0007215	biological_process	glutamate receptor signaling pathway
B	0007268	biological_process	chemical synaptic transmission
B	0007420	biological_process	brain development
B	0007611	biological_process	learning or memory
B	0007613	biological_process	memory
B	0007625	biological_process	grooming behavior
B	0007626	biological_process	locomotory behavior
B	0009410	biological_process	response to xenobiotic stimulus
B	0009986	cellular_component	cell surface
B	0010460	biological_process	positive regulation of heart rate
B	0010467	biological_process	gene expression
B	0010842	biological_process	retina layer formation
B	0015108	molecular_function	chloride transmembrane transporter activity
B	0015183	molecular_function	L-aspartate transmembrane transporter activity
B	0015293	molecular_function	symporter activity
B	0015501	molecular_function	glutamate:sodium symporter activity
B	0015813	biological_process	L-glutamate transmembrane transport
B	0016020	cellular_component	membrane
B	0016324	cellular_component	apical plasma membrane
B	0019221	biological_process	cytokine-mediated signaling pathway
B	0022008	biological_process	neurogenesis
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0030534	biological_process	adult behavior
B	0031901	cellular_component	early endosome membrane
B	0031902	cellular_component	late endosome membrane
B	0031982	cellular_component	vesicle
B	0032279	cellular_component	asymmetric synapse
B	0033229	molecular_function	cysteine transmembrane transporter activity
B	0034599	biological_process	cellular response to oxidative stress
B	0035633	biological_process	maintenance of blood-brain barrier
B	0036293	biological_process	response to decreased oxygen levels
B	0042417	biological_process	dopamine metabolic process
B	0042802	molecular_function	identical protein binding
B	0042883	biological_process	cysteine transport
B	0043005	cellular_component	neuron projection
B	0043025	cellular_component	neuronal cell body
B	0043083	cellular_component	synaptic cleft
B	0043197	cellular_component	dendritic spine
B	0043198	cellular_component	dendritic shaft
B	0043204	cellular_component	perikaryon
B	0043278	biological_process	response to morphine
B	0043524	biological_process	negative regulation of neuron apoptotic process
B	0043679	cellular_component	axon terminus
B	0045121	cellular_component	membrane raft
B	0045184	biological_process	establishment of protein localization
B	0045202	cellular_component	synapse
B	0046872	molecular_function	metal ion binding
B	0048514	biological_process	blood vessel morphogenesis
B	0048678	biological_process	response to axon injury
B	0050808	biological_process	synapse organization
B	0051402	biological_process	neuron apoptotic process
B	0051938	biological_process	L-glutamate import
B	0055037	cellular_component	recycling endosome
B	0055038	cellular_component	recycling endosome membrane
B	0060013	biological_process	righting reflex
B	0060041	biological_process	retina development in camera-type eye
B	0060047	biological_process	heart contraction
B	0060291	biological_process	long-term synaptic potentiation
B	0061744	biological_process	motor behavior
B	0070062	cellular_component	extracellular exosome
B	0070633	biological_process	transepithelial transport
B	0070777	biological_process	D-aspartate transmembrane transport
B	0070778	biological_process	L-aspartate transmembrane transport
B	0070779	biological_process	D-aspartate import across plasma membrane
B	0071242	biological_process	cellular response to ammonium ion
B	0071288	biological_process	cellular response to mercury ion
B	0071314	biological_process	cellular response to cocaine
B	0071407	biological_process	obsolete cellular response to organic cyclic compound
B	0071577	biological_process	zinc ion transmembrane transport
B	0071944	cellular_component	cell periphery
B	0072347	biological_process	response to anesthetic
B	0090313	biological_process	regulation of protein targeting to membrane
B	0090461	biological_process	intracellular glutamate homeostasis
B	0097049	biological_process	motor neuron apoptotic process
B	0097386	cellular_component	glial cell projection
B	0097440	cellular_component	apical dendrite
B	0098657	biological_process	import into cell
B	0098685	cellular_component	Schaffer collateral - CA1 synapse
B	0098712	biological_process	L-glutamate import across plasma membrane
B	0098793	cellular_component	presynapse
B	0098877	biological_process	neurotransmitter receptor transport to plasma membrane
B	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
B	0099544	cellular_component	perisynaptic space
B	0140009	biological_process	L-aspartate import across plasma membrane
B	0140010	molecular_function	D-aspartate transmembrane transporter activity
B	0150002	cellular_component	distal dendrite
B	0150104	biological_process	transport across blood-brain barrier
B	1902476	biological_process	chloride transmembrane transport
B	1903712	biological_process	cysteine transmembrane transport
B	1903926	biological_process	cellular response to bisphenol A
B	1990635	cellular_component	proximal dendrite
B	1990708	biological_process	conditioned place preference
C	0001662	biological_process	behavioral fear response
C	0001975	biological_process	response to amphetamine
C	0002027	biological_process	regulation of heart rate
C	0005253	molecular_function	monoatomic anion channel activity
C	0005313	molecular_function	L-glutamate transmembrane transporter activity
C	0005314	molecular_function	high-affinity L-glutamate transmembrane transporter activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005768	cellular_component	endosome
C	0005886	cellular_component	plasma membrane
C	0006749	biological_process	glutathione metabolic process
C	0006750	biological_process	glutathione biosynthetic process
C	0006801	biological_process	superoxide metabolic process
C	0006811	biological_process	monoatomic ion transport
C	0006836	biological_process	neurotransmitter transport
C	0006865	biological_process	amino acid transport
C	0006882	biological_process	intracellular zinc ion homeostasis
C	0007212	biological_process	G protein-coupled dopamine receptor signaling pathway
C	0007215	biological_process	glutamate receptor signaling pathway
C	0007268	biological_process	chemical synaptic transmission
C	0007420	biological_process	brain development
C	0007611	biological_process	learning or memory
C	0007613	biological_process	memory
C	0007625	biological_process	grooming behavior
C	0007626	biological_process	locomotory behavior
C	0009410	biological_process	response to xenobiotic stimulus
C	0009986	cellular_component	cell surface
C	0010460	biological_process	positive regulation of heart rate
C	0010467	biological_process	gene expression
C	0010842	biological_process	retina layer formation
C	0015108	molecular_function	chloride transmembrane transporter activity
C	0015183	molecular_function	L-aspartate transmembrane transporter activity
C	0015293	molecular_function	symporter activity
C	0015501	molecular_function	glutamate:sodium symporter activity
C	0015813	biological_process	L-glutamate transmembrane transport
C	0016020	cellular_component	membrane
C	0016324	cellular_component	apical plasma membrane
C	0019221	biological_process	cytokine-mediated signaling pathway
C	0022008	biological_process	neurogenesis
C	0030424	cellular_component	axon
C	0030425	cellular_component	dendrite
C	0030534	biological_process	adult behavior
C	0031901	cellular_component	early endosome membrane
C	0031902	cellular_component	late endosome membrane
C	0031982	cellular_component	vesicle
C	0032279	cellular_component	asymmetric synapse
C	0033229	molecular_function	cysteine transmembrane transporter activity
C	0034599	biological_process	cellular response to oxidative stress
C	0035633	biological_process	maintenance of blood-brain barrier
C	0036293	biological_process	response to decreased oxygen levels
C	0042417	biological_process	dopamine metabolic process
C	0042802	molecular_function	identical protein binding
C	0042883	biological_process	cysteine transport
C	0043005	cellular_component	neuron projection
C	0043025	cellular_component	neuronal cell body
C	0043083	cellular_component	synaptic cleft
C	0043197	cellular_component	dendritic spine
C	0043198	cellular_component	dendritic shaft
C	0043204	cellular_component	perikaryon
C	0043278	biological_process	response to morphine
C	0043524	biological_process	negative regulation of neuron apoptotic process
C	0043679	cellular_component	axon terminus
C	0045121	cellular_component	membrane raft
C	0045184	biological_process	establishment of protein localization
C	0045202	cellular_component	synapse
C	0046872	molecular_function	metal ion binding
C	0048514	biological_process	blood vessel morphogenesis
C	0048678	biological_process	response to axon injury
C	0050808	biological_process	synapse organization
C	0051402	biological_process	neuron apoptotic process
C	0051938	biological_process	L-glutamate import
C	0055037	cellular_component	recycling endosome
C	0055038	cellular_component	recycling endosome membrane
C	0060013	biological_process	righting reflex
C	0060041	biological_process	retina development in camera-type eye
C	0060047	biological_process	heart contraction
C	0060291	biological_process	long-term synaptic potentiation
C	0061744	biological_process	motor behavior
C	0070062	cellular_component	extracellular exosome
C	0070633	biological_process	transepithelial transport
C	0070777	biological_process	D-aspartate transmembrane transport
C	0070778	biological_process	L-aspartate transmembrane transport
C	0070779	biological_process	D-aspartate import across plasma membrane
C	0071242	biological_process	cellular response to ammonium ion
C	0071288	biological_process	cellular response to mercury ion
C	0071314	biological_process	cellular response to cocaine
C	0071407	biological_process	obsolete cellular response to organic cyclic compound
C	0071577	biological_process	zinc ion transmembrane transport
C	0071944	cellular_component	cell periphery
C	0072347	biological_process	response to anesthetic
C	0090313	biological_process	regulation of protein targeting to membrane
C	0090461	biological_process	intracellular glutamate homeostasis
C	0097049	biological_process	motor neuron apoptotic process
C	0097386	cellular_component	glial cell projection
C	0097440	cellular_component	apical dendrite
C	0098657	biological_process	import into cell
C	0098685	cellular_component	Schaffer collateral - CA1 synapse
C	0098712	biological_process	L-glutamate import across plasma membrane
C	0098793	cellular_component	presynapse
C	0098877	biological_process	neurotransmitter receptor transport to plasma membrane
C	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
C	0099544	cellular_component	perisynaptic space
C	0140009	biological_process	L-aspartate import across plasma membrane
C	0140010	molecular_function	D-aspartate transmembrane transporter activity
C	0150002	cellular_component	distal dendrite
C	0150104	biological_process	transport across blood-brain barrier
C	1902476	biological_process	chloride transmembrane transport
C	1903712	biological_process	cysteine transmembrane transport
C	1903926	biological_process	cellular response to bisphenol A
C	1990635	cellular_component	proximal dendrite
C	1990708	biological_process	conditioned place preference

Functional Information from PROSITE/UniProt

site_id	PS00713
Number of Residues	15
Details	NA_DICARBOXYL_SYMP_1 Sodium:dicarboxylate symporter family signature 1. P.GEiLMrMLKLIIlP

Chain	Residue	Details
A	PRO55-PRO69

---

site_id	PS00714
Number of Residues	24
Details	NA_DICARBOXYL_SYMP_2 Sodium:dicarboxylate symporter family signature 2. PvGaTiNMDGTaLYeaVaaVFIAQ

Chain	Residue	Details
A	PRO360-GLN383

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	177
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	195
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	90
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	69
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	81
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	63
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	189
Details	Intramembrane: {"description":"Discontinuously helical","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	78
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	63
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	39
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33658209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6X2Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33658209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6X2Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6X3E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33658209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6X3E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---