Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8CBM

Structure of human mitochondrial CCA-adding enzyme in complex with mitochondrial pre-tRNA-Ile

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0003723	molecular_function	RNA binding
A	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
A	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)+] activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005886	cellular_component	plasma membrane
A	0006550	biological_process	isoleucine catabolic process
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0006699	biological_process	bile acid biosynthetic process
A	0007005	biological_process	mitochondrion organization
A	0008033	biological_process	tRNA processing
A	0008202	biological_process	steroid metabolic process
A	0008207	biological_process	C21-steroid hormone metabolic process
A	0008209	biological_process	androgen metabolic process
A	0008210	biological_process	estrogen metabolic process
A	0008709	molecular_function	cholate 7-alpha-dehydrogenase activity
A	0016491	molecular_function	oxidoreductase activity
A	0030283	molecular_function	testosterone dehydrogenase [NAD(P)+] activity
A	0030678	cellular_component	mitochondrial ribonuclease P complex
A	0042645	cellular_component	mitochondrial nucleoid
A	0043527	cellular_component	tRNA methyltransferase complex
A	0044594	molecular_function	17-beta-hydroxysteroid dehydrogenase (NAD+) activity
A	0047015	molecular_function	3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
A	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
A	0047044	molecular_function	androstan-3-alpha,17-beta-diol dehydrogenase activity
A	0051289	biological_process	protein homotetramerization
A	0062173	biological_process	brexanolone metabolic process
A	0070901	biological_process	mitochondrial tRNA methylation
A	0097745	biological_process	mitochondrial tRNA 5'-end processing
A	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
A	0106282	molecular_function	isoursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
A	0106283	molecular_function	ursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
A	1990180	biological_process	mitochondrial tRNA 3'-end processing
B	0000049	molecular_function	tRNA binding
B	0003723	molecular_function	RNA binding
B	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
B	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)+] activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005886	cellular_component	plasma membrane
B	0006550	biological_process	isoleucine catabolic process
B	0006629	biological_process	lipid metabolic process
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0006699	biological_process	bile acid biosynthetic process
B	0007005	biological_process	mitochondrion organization
B	0008033	biological_process	tRNA processing
B	0008202	biological_process	steroid metabolic process
B	0008207	biological_process	C21-steroid hormone metabolic process
B	0008209	biological_process	androgen metabolic process
B	0008210	biological_process	estrogen metabolic process
B	0008709	molecular_function	cholate 7-alpha-dehydrogenase activity
B	0016491	molecular_function	oxidoreductase activity
B	0030283	molecular_function	testosterone dehydrogenase [NAD(P)+] activity
B	0030678	cellular_component	mitochondrial ribonuclease P complex
B	0042645	cellular_component	mitochondrial nucleoid
B	0043527	cellular_component	tRNA methyltransferase complex
B	0044594	molecular_function	17-beta-hydroxysteroid dehydrogenase (NAD+) activity
B	0047015	molecular_function	3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
B	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
B	0047044	molecular_function	androstan-3-alpha,17-beta-diol dehydrogenase activity
B	0051289	biological_process	protein homotetramerization
B	0062173	biological_process	brexanolone metabolic process
B	0070901	biological_process	mitochondrial tRNA methylation
B	0097745	biological_process	mitochondrial tRNA 5'-end processing
B	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
B	0106282	molecular_function	isoursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
B	0106283	molecular_function	ursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
B	1990180	biological_process	mitochondrial tRNA 3'-end processing
C	0000049	molecular_function	tRNA binding
C	0003723	molecular_function	RNA binding
C	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
C	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)+] activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005886	cellular_component	plasma membrane
C	0006550	biological_process	isoleucine catabolic process
C	0006629	biological_process	lipid metabolic process
C	0006631	biological_process	fatty acid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0006699	biological_process	bile acid biosynthetic process
C	0007005	biological_process	mitochondrion organization
C	0008033	biological_process	tRNA processing
C	0008202	biological_process	steroid metabolic process
C	0008207	biological_process	C21-steroid hormone metabolic process
C	0008209	biological_process	androgen metabolic process
C	0008210	biological_process	estrogen metabolic process
C	0008709	molecular_function	cholate 7-alpha-dehydrogenase activity
C	0016491	molecular_function	oxidoreductase activity
C	0030283	molecular_function	testosterone dehydrogenase [NAD(P)+] activity
C	0030678	cellular_component	mitochondrial ribonuclease P complex
C	0042645	cellular_component	mitochondrial nucleoid
C	0043527	cellular_component	tRNA methyltransferase complex
C	0044594	molecular_function	17-beta-hydroxysteroid dehydrogenase (NAD+) activity
C	0047015	molecular_function	3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
C	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
C	0047044	molecular_function	androstan-3-alpha,17-beta-diol dehydrogenase activity
C	0051289	biological_process	protein homotetramerization
C	0062173	biological_process	brexanolone metabolic process
C	0070901	biological_process	mitochondrial tRNA methylation
C	0097745	biological_process	mitochondrial tRNA 5'-end processing
C	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
C	0106282	molecular_function	isoursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
C	0106283	molecular_function	ursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
C	1990180	biological_process	mitochondrial tRNA 3'-end processing
D	0000049	molecular_function	tRNA binding
D	0003723	molecular_function	RNA binding
D	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
D	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)+] activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0005886	cellular_component	plasma membrane
D	0006550	biological_process	isoleucine catabolic process
D	0006629	biological_process	lipid metabolic process
D	0006631	biological_process	fatty acid metabolic process
D	0006635	biological_process	fatty acid beta-oxidation
D	0006699	biological_process	bile acid biosynthetic process
D	0007005	biological_process	mitochondrion organization
D	0008033	biological_process	tRNA processing
D	0008202	biological_process	steroid metabolic process
D	0008207	biological_process	C21-steroid hormone metabolic process
D	0008209	biological_process	androgen metabolic process
D	0008210	biological_process	estrogen metabolic process
D	0008709	molecular_function	cholate 7-alpha-dehydrogenase activity
D	0016491	molecular_function	oxidoreductase activity
D	0030283	molecular_function	testosterone dehydrogenase [NAD(P)+] activity
D	0030678	cellular_component	mitochondrial ribonuclease P complex
D	0042645	cellular_component	mitochondrial nucleoid
D	0043527	cellular_component	tRNA methyltransferase complex
D	0044594	molecular_function	17-beta-hydroxysteroid dehydrogenase (NAD+) activity
D	0047015	molecular_function	3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
D	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
D	0047044	molecular_function	androstan-3-alpha,17-beta-diol dehydrogenase activity
D	0051289	biological_process	protein homotetramerization
D	0062173	biological_process	brexanolone metabolic process
D	0070901	biological_process	mitochondrial tRNA methylation
D	0097745	biological_process	mitochondrial tRNA 5'-end processing
D	0106281	molecular_function	chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
D	0106282	molecular_function	isoursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
D	0106283	molecular_function	ursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
D	1990180	biological_process	mitochondrial tRNA 3'-end processing
E	0000049	molecular_function	tRNA binding
E	0001680	biological_process	tRNA 3'-terminal CCA addition
E	0003723	molecular_function	RNA binding
E	0004810	molecular_function	CCA tRNA nucleotidyltransferase activity
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0005829	cellular_component	cytosol
E	0008033	biological_process	tRNA processing
E	0016779	molecular_function	nucleotidyltransferase activity
E	0034062	molecular_function	5'-3' RNA polymerase activity
E	0042780	biological_process	tRNA 3'-end processing
E	0042803	molecular_function	protein homodimerization activity
E	0046872	molecular_function	metal ion binding
E	0072344	biological_process	rescue of stalled ribosome
E	0106354	biological_process	tRNA surveillance
E	0160016	molecular_function	CCACCA tRNA nucleotidyltransferase activity
E	1990180	biological_process	mitochondrial tRNA 3'-end processing
F	0000049	molecular_function	tRNA binding
F	0000964	biological_process	mitochondrial RNA 5'-end processing
F	0003723	molecular_function	RNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0006397	biological_process	mRNA processing
F	0008033	biological_process	tRNA processing
F	0008168	molecular_function	methyltransferase activity
F	0030678	cellular_component	mitochondrial ribonuclease P complex
F	0032259	biological_process	methylation
F	0042645	cellular_component	mitochondrial nucleoid
F	0042802	molecular_function	identical protein binding
F	0043527	cellular_component	tRNA methyltransferase complex
F	0052905	molecular_function	tRNA (guanosine(9)-N1)-methyltransferase activity
F	0070131	biological_process	positive regulation of mitochondrial translation
F	0070901	biological_process	mitochondrial tRNA methylation
F	0090646	biological_process	mitochondrial tRNA processing
F	0097745	biological_process	mitochondrial tRNA 5'-end processing
F	0160106	molecular_function	tRNA (adenine(9)-N1)-methyltransferase activity
F	1990180	biological_process	mitochondrial tRNA 3'-end processing

Functional Information from PROSITE/UniProt

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. SvaafegqvgQaaYSASKGGIvGMTlPIA

Chain	Residue	Details
A	SER155-ALA183

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q3UFY8

Chain	Residue	Details
F	SER84
E	ARG67
E	ARG151
E	ASP194
E	ARG197
E	ARG200
E	ARG203

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:O66728

Chain	Residue	Details
E	ASP77
A	THR203
B	SER20
B	LEU22
B	ASP41
B	ASP64
B	VAL65
B	CYS91
B	TYR168
B	LYS172
B	PHE201
E	ASP79
B	THR203
C	SER20
C	LEU22
C	ASP41
C	ASP64
C	VAL65
C	CYS91
C	TYR168
C	LYS172
C	PHE201
A	ASP41
C	THR203
D	SER20
D	LEU22
D	ASP41
D	ASP64
D	VAL65
D	CYS91
D	TYR168
D	LYS172
D	PHE201
A	ASP64
D	THR203
A	VAL65
A	CYS91
A	TYR168
A	LYS172
A	PHE201

site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: May assist in discriminating ATP from CTP => ECO:0000250\|UniProtKB:Q7SIB1

Chain	Residue	Details
E	ASP152
B	SER155
C	SER155
D	SER155

site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Involved in nucleotide selection => ECO:0000255

Chain	Residue	Details
E	GLU193
B	ALA2
C	ALA2
D	ALA2

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
E	SER400
A	LYS212
B	LYS53
B	LYS212
C	LYS53
C	LYS212
D	LYS53
D	LYS212

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
E	LYS402
D	LYS69
D	LYS99
D	LYS105
A	LYS99
A	LYS105
B	LYS69
B	LYS99
B	LYS105
C	LYS69
C	LYS99
C	LYS105

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)