8AVF

Human leptin in complex with the human LEP-R ectodomain fused to a C-terminal trimeric isoleucine GCN4 zipper (closed 3:3 model)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0001525	biological_process	angiogenesis
A	0001542	biological_process	ovulation from ovarian follicle
A	0001666	biological_process	response to hypoxia
A	0001819	biological_process	positive regulation of cytokine production
A	0001890	biological_process	placenta development
A	0001934	biological_process	positive regulation of protein phosphorylation
A	0001936	biological_process	regulation of endothelial cell proliferation
A	0002021	biological_process	response to dietary excess
A	0003300	biological_process	cardiac muscle hypertrophy
A	0003677	molecular_function	DNA binding
A	0005102	molecular_function	signaling receptor binding
A	0005179	molecular_function	hormone activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006006	biological_process	glucose metabolic process
A	0006111	biological_process	regulation of gluconeogenesis
A	0006112	biological_process	energy reserve metabolic process
A	0006114	biological_process	glycerol biosynthetic process
A	0006629	biological_process	lipid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0006909	biological_process	phagocytosis
A	0007165	biological_process	signal transduction
A	0007259	biological_process	cell surface receptor signaling pathway via JAK-STAT
A	0007565	biological_process	female pregnancy
A	0007584	biological_process	response to nutrient
A	0007623	biological_process	circadian rhythm
A	0008203	biological_process	cholesterol metabolic process
A	0008206	biological_process	bile acid metabolic process
A	0008217	biological_process	regulation of blood pressure
A	0008284	biological_process	positive regulation of cell population proliferation
A	0008340	biological_process	determination of adult lifespan
A	0008343	biological_process	adult feeding behavior
A	0009062	biological_process	fatty acid catabolic process
A	0009892	biological_process	negative regulation of metabolic process
A	0010507	biological_process	negative regulation of autophagy
A	0010888	biological_process	negative regulation of lipid storage
A	0014823	biological_process	response to activity
A	0019222	biological_process	regulation of metabolic process
A	0019953	biological_process	sexual reproduction
A	0021954	biological_process	central nervous system neuron development
A	0030073	biological_process	insulin secretion
A	0030217	biological_process	T cell differentiation
A	0030300	biological_process	regulation of intestinal cholesterol absorption
A	0031667	biological_process	response to nutrient levels
A	0032008	biological_process	positive regulation of TOR signaling
A	0032099	biological_process	negative regulation of appetite
A	0032310	biological_process	prostaglandin secretion
A	0032355	biological_process	response to estradiol
A	0032735	biological_process	positive regulation of interleukin-12 production
A	0032755	biological_process	positive regulation of interleukin-6 production
A	0032757	biological_process	positive regulation of interleukin-8 production
A	0032760	biological_process	positive regulation of tumor necrosis factor production
A	0032814	biological_process	regulation of natural killer cell activation
A	0032817	biological_process	regulation of natural killer cell proliferation
A	0032868	biological_process	response to insulin
A	0032869	biological_process	cellular response to insulin stimulus
A	0033197	biological_process	response to vitamin E
A	0033210	biological_process	leptin-mediated signaling pathway
A	0033686	biological_process	positive regulation of luteinizing hormone secretion
A	0035360	biological_process	positive regulation of peroxisome proliferator activated receptor signaling pathway
A	0035556	biological_process	intracellular signal transduction
A	0035630	biological_process	bone mineralization involved in bone maturation
A	0035904	biological_process	aorta development
A	0038108	biological_process	negative regulation of appetite by leptin-mediated signaling pathway
A	0042102	biological_process	positive regulation of T cell proliferation
A	0042269	biological_process	regulation of natural killer cell mediated cytotoxicity
A	0042307	biological_process	positive regulation of protein import into nucleus
A	0042445	biological_process	hormone metabolic process
A	0042593	biological_process	glucose homeostasis
A	0042755	biological_process	eating behavior
A	0043066	biological_process	negative regulation of apoptotic process
A	0043270	biological_process	positive regulation of monoatomic ion transport
A	0043410	biological_process	positive regulation of MAPK cascade
A	0044320	biological_process	cellular response to leptin stimulus
A	0045471	biological_process	response to ethanol
A	0045598	biological_process	regulation of fat cell differentiation
A	0045765	biological_process	regulation of angiogenesis
A	0045906	biological_process	negative regulation of vasoconstriction
A	0046325	biological_process	negative regulation of D-glucose import
A	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
A	0046628	biological_process	positive regulation of insulin receptor signaling pathway
A	0046850	biological_process	regulation of bone remodeling
A	0046881	biological_process	positive regulation of follicle-stimulating hormone secretion
A	0046890	biological_process	regulation of lipid biosynthetic process
A	0048639	biological_process	positive regulation of developmental growth
A	0050796	biological_process	regulation of insulin secretion
A	0050810	biological_process	regulation of steroid biosynthetic process
A	0050892	biological_process	intestinal absorption
A	0050901	biological_process	leukocyte tethering or rolling
A	0050999	biological_process	regulation of nitric-oxide synthase activity
A	0051428	molecular_function	peptide hormone receptor binding
A	0051541	biological_process	elastin metabolic process
A	0051726	biological_process	regulation of cell cycle
A	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
A	0060587	biological_process	regulation of lipoprotein lipid oxidation
A	0060612	biological_process	adipose tissue development
A	0061037	biological_process	negative regulation of cartilage development
A	0070093	biological_process	negative regulation of glucagon secretion
A	0071298	biological_process	cellular response to L-ascorbic acid
A	0071300	biological_process	cellular response to retinoic acid
A	0090335	biological_process	regulation of brown fat cell differentiation
A	0097009	biological_process	energy homeostasis
A	0098868	biological_process	bone growth
A	0120162	biological_process	positive regulation of cold-induced thermogenesis
A	1900015	biological_process	regulation of cytokine production involved in inflammatory response
A	1900180	biological_process	regulation of protein localization to nucleus
A	1900745	biological_process	positive regulation of p38MAPK cascade
A	1904651	biological_process	positive regulation of fat cell apoptotic process
A	1990051	biological_process	activation of protein kinase C activity
A	1990460	molecular_function	leptin receptor binding
A	2000379	biological_process	positive regulation of reactive oxygen species metabolic process
A	2000486	biological_process	negative regulation of glutamine transport
A	2000491	biological_process	positive regulation of hepatic stellate cell activation
B	0003700	molecular_function	DNA-binding transcription factor activity
B	0004896	molecular_function	cytokine receptor activity
B	0006355	biological_process	regulation of DNA-templated transcription
B	0016020	cellular_component	membrane
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0001525	biological_process	angiogenesis
C	0001542	biological_process	ovulation from ovarian follicle
C	0001666	biological_process	response to hypoxia
C	0001819	biological_process	positive regulation of cytokine production
C	0001890	biological_process	placenta development
C	0001934	biological_process	positive regulation of protein phosphorylation
C	0001936	biological_process	regulation of endothelial cell proliferation
C	0002021	biological_process	response to dietary excess
C	0003300	biological_process	cardiac muscle hypertrophy
C	0003677	molecular_function	DNA binding
C	0005102	molecular_function	signaling receptor binding
C	0005179	molecular_function	hormone activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006006	biological_process	glucose metabolic process
C	0006111	biological_process	regulation of gluconeogenesis
C	0006112	biological_process	energy reserve metabolic process
C	0006114	biological_process	glycerol biosynthetic process
C	0006629	biological_process	lipid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0006909	biological_process	phagocytosis
C	0007165	biological_process	signal transduction
C	0007259	biological_process	cell surface receptor signaling pathway via JAK-STAT
C	0007565	biological_process	female pregnancy
C	0007584	biological_process	response to nutrient
C	0007623	biological_process	circadian rhythm
C	0008203	biological_process	cholesterol metabolic process
C	0008206	biological_process	bile acid metabolic process
C	0008217	biological_process	regulation of blood pressure
C	0008284	biological_process	positive regulation of cell population proliferation
C	0008340	biological_process	determination of adult lifespan
C	0008343	biological_process	adult feeding behavior
C	0009062	biological_process	fatty acid catabolic process
C	0009892	biological_process	negative regulation of metabolic process
C	0010507	biological_process	negative regulation of autophagy
C	0010888	biological_process	negative regulation of lipid storage
C	0014823	biological_process	response to activity
C	0019222	biological_process	regulation of metabolic process
C	0019953	biological_process	sexual reproduction
C	0021954	biological_process	central nervous system neuron development
C	0030073	biological_process	insulin secretion
C	0030217	biological_process	T cell differentiation
C	0030300	biological_process	regulation of intestinal cholesterol absorption
C	0031667	biological_process	response to nutrient levels
C	0032008	biological_process	positive regulation of TOR signaling
C	0032099	biological_process	negative regulation of appetite
C	0032310	biological_process	prostaglandin secretion
C	0032355	biological_process	response to estradiol
C	0032735	biological_process	positive regulation of interleukin-12 production
C	0032755	biological_process	positive regulation of interleukin-6 production
C	0032757	biological_process	positive regulation of interleukin-8 production
C	0032760	biological_process	positive regulation of tumor necrosis factor production
C	0032814	biological_process	regulation of natural killer cell activation
C	0032817	biological_process	regulation of natural killer cell proliferation
C	0032868	biological_process	response to insulin
C	0032869	biological_process	cellular response to insulin stimulus
C	0033197	biological_process	response to vitamin E
C	0033210	biological_process	leptin-mediated signaling pathway
C	0033686	biological_process	positive regulation of luteinizing hormone secretion
C	0035360	biological_process	positive regulation of peroxisome proliferator activated receptor signaling pathway
C	0035556	biological_process	intracellular signal transduction
C	0035630	biological_process	bone mineralization involved in bone maturation
C	0035904	biological_process	aorta development
C	0038108	biological_process	negative regulation of appetite by leptin-mediated signaling pathway
C	0042102	biological_process	positive regulation of T cell proliferation
C	0042269	biological_process	regulation of natural killer cell mediated cytotoxicity
C	0042307	biological_process	positive regulation of protein import into nucleus
C	0042445	biological_process	hormone metabolic process
C	0042593	biological_process	glucose homeostasis
C	0042755	biological_process	eating behavior
C	0043066	biological_process	negative regulation of apoptotic process
C	0043270	biological_process	positive regulation of monoatomic ion transport
C	0043410	biological_process	positive regulation of MAPK cascade
C	0044320	biological_process	cellular response to leptin stimulus
C	0045471	biological_process	response to ethanol
C	0045598	biological_process	regulation of fat cell differentiation
C	0045765	biological_process	regulation of angiogenesis
C	0045906	biological_process	negative regulation of vasoconstriction
C	0046325	biological_process	negative regulation of D-glucose import
C	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
C	0046628	biological_process	positive regulation of insulin receptor signaling pathway
C	0046850	biological_process	regulation of bone remodeling
C	0046881	biological_process	positive regulation of follicle-stimulating hormone secretion
C	0046890	biological_process	regulation of lipid biosynthetic process
C	0048639	biological_process	positive regulation of developmental growth
C	0050796	biological_process	regulation of insulin secretion
C	0050810	biological_process	regulation of steroid biosynthetic process
C	0050892	biological_process	intestinal absorption
C	0050901	biological_process	leukocyte tethering or rolling
C	0050999	biological_process	regulation of nitric-oxide synthase activity
C	0051428	molecular_function	peptide hormone receptor binding
C	0051541	biological_process	elastin metabolic process
C	0051726	biological_process	regulation of cell cycle
C	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
C	0060587	biological_process	regulation of lipoprotein lipid oxidation
C	0060612	biological_process	adipose tissue development
C	0061037	biological_process	negative regulation of cartilage development
C	0070093	biological_process	negative regulation of glucagon secretion
C	0071298	biological_process	cellular response to L-ascorbic acid
C	0071300	biological_process	cellular response to retinoic acid
C	0090335	biological_process	regulation of brown fat cell differentiation
C	0097009	biological_process	energy homeostasis
C	0098868	biological_process	bone growth
C	0120162	biological_process	positive regulation of cold-induced thermogenesis
C	1900015	biological_process	regulation of cytokine production involved in inflammatory response
C	1900180	biological_process	regulation of protein localization to nucleus
C	1900745	biological_process	positive regulation of p38MAPK cascade
C	1904651	biological_process	positive regulation of fat cell apoptotic process
C	1990051	biological_process	activation of protein kinase C activity
C	1990460	molecular_function	leptin receptor binding
C	2000379	biological_process	positive regulation of reactive oxygen species metabolic process
C	2000486	biological_process	negative regulation of glutamine transport
C	2000491	biological_process	positive regulation of hepatic stellate cell activation
D	0003700	molecular_function	DNA-binding transcription factor activity
D	0004896	molecular_function	cytokine receptor activity
D	0006355	biological_process	regulation of DNA-templated transcription
D	0016020	cellular_component	membrane
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0001525	biological_process	angiogenesis
E	0001542	biological_process	ovulation from ovarian follicle
E	0001666	biological_process	response to hypoxia
E	0001819	biological_process	positive regulation of cytokine production
E	0001890	biological_process	placenta development
E	0001934	biological_process	positive regulation of protein phosphorylation
E	0001936	biological_process	regulation of endothelial cell proliferation
E	0002021	biological_process	response to dietary excess
E	0003300	biological_process	cardiac muscle hypertrophy
E	0003677	molecular_function	DNA binding
E	0005102	molecular_function	signaling receptor binding
E	0005179	molecular_function	hormone activity
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006006	biological_process	glucose metabolic process
E	0006111	biological_process	regulation of gluconeogenesis
E	0006112	biological_process	energy reserve metabolic process
E	0006114	biological_process	glycerol biosynthetic process
E	0006629	biological_process	lipid metabolic process
E	0006635	biological_process	fatty acid beta-oxidation
E	0006909	biological_process	phagocytosis
E	0007165	biological_process	signal transduction
E	0007259	biological_process	cell surface receptor signaling pathway via JAK-STAT
E	0007565	biological_process	female pregnancy
E	0007584	biological_process	response to nutrient
E	0007623	biological_process	circadian rhythm
E	0008203	biological_process	cholesterol metabolic process
E	0008206	biological_process	bile acid metabolic process
E	0008217	biological_process	regulation of blood pressure
E	0008284	biological_process	positive regulation of cell population proliferation
E	0008340	biological_process	determination of adult lifespan
E	0008343	biological_process	adult feeding behavior
E	0009062	biological_process	fatty acid catabolic process
E	0009892	biological_process	negative regulation of metabolic process
E	0010507	biological_process	negative regulation of autophagy
E	0010888	biological_process	negative regulation of lipid storage
E	0014823	biological_process	response to activity
E	0019222	biological_process	regulation of metabolic process
E	0019953	biological_process	sexual reproduction
E	0021954	biological_process	central nervous system neuron development
E	0030073	biological_process	insulin secretion
E	0030217	biological_process	T cell differentiation
E	0030300	biological_process	regulation of intestinal cholesterol absorption
E	0031667	biological_process	response to nutrient levels
E	0032008	biological_process	positive regulation of TOR signaling
E	0032099	biological_process	negative regulation of appetite
E	0032310	biological_process	prostaglandin secretion
E	0032355	biological_process	response to estradiol
E	0032735	biological_process	positive regulation of interleukin-12 production
E	0032755	biological_process	positive regulation of interleukin-6 production
E	0032757	biological_process	positive regulation of interleukin-8 production
E	0032760	biological_process	positive regulation of tumor necrosis factor production
E	0032814	biological_process	regulation of natural killer cell activation
E	0032817	biological_process	regulation of natural killer cell proliferation
E	0032868	biological_process	response to insulin
E	0032869	biological_process	cellular response to insulin stimulus
E	0033197	biological_process	response to vitamin E
E	0033210	biological_process	leptin-mediated signaling pathway
E	0033686	biological_process	positive regulation of luteinizing hormone secretion
E	0035360	biological_process	positive regulation of peroxisome proliferator activated receptor signaling pathway
E	0035556	biological_process	intracellular signal transduction
E	0035630	biological_process	bone mineralization involved in bone maturation
E	0035904	biological_process	aorta development
E	0038108	biological_process	negative regulation of appetite by leptin-mediated signaling pathway
E	0042102	biological_process	positive regulation of T cell proliferation
E	0042269	biological_process	regulation of natural killer cell mediated cytotoxicity
E	0042307	biological_process	positive regulation of protein import into nucleus
E	0042445	biological_process	hormone metabolic process
E	0042593	biological_process	glucose homeostasis
E	0042755	biological_process	eating behavior
E	0043066	biological_process	negative regulation of apoptotic process
E	0043270	biological_process	positive regulation of monoatomic ion transport
E	0043410	biological_process	positive regulation of MAPK cascade
E	0044320	biological_process	cellular response to leptin stimulus
E	0045471	biological_process	response to ethanol
E	0045598	biological_process	regulation of fat cell differentiation
E	0045765	biological_process	regulation of angiogenesis
E	0045906	biological_process	negative regulation of vasoconstriction
E	0046325	biological_process	negative regulation of D-glucose import
E	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
E	0046628	biological_process	positive regulation of insulin receptor signaling pathway
E	0046850	biological_process	regulation of bone remodeling
E	0046881	biological_process	positive regulation of follicle-stimulating hormone secretion
E	0046890	biological_process	regulation of lipid biosynthetic process
E	0048639	biological_process	positive regulation of developmental growth
E	0050796	biological_process	regulation of insulin secretion
E	0050810	biological_process	regulation of steroid biosynthetic process
E	0050892	biological_process	intestinal absorption
E	0050901	biological_process	leukocyte tethering or rolling
E	0050999	biological_process	regulation of nitric-oxide synthase activity
E	0051428	molecular_function	peptide hormone receptor binding
E	0051541	biological_process	elastin metabolic process
E	0051726	biological_process	regulation of cell cycle
E	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
E	0060587	biological_process	regulation of lipoprotein lipid oxidation
E	0060612	biological_process	adipose tissue development
E	0061037	biological_process	negative regulation of cartilage development
E	0070093	biological_process	negative regulation of glucagon secretion
E	0071298	biological_process	cellular response to L-ascorbic acid
E	0071300	biological_process	cellular response to retinoic acid
E	0090335	biological_process	regulation of brown fat cell differentiation
E	0097009	biological_process	energy homeostasis
E	0098868	biological_process	bone growth
E	0120162	biological_process	positive regulation of cold-induced thermogenesis
E	1900015	biological_process	regulation of cytokine production involved in inflammatory response
E	1900180	biological_process	regulation of protein localization to nucleus
E	1900745	biological_process	positive regulation of p38MAPK cascade
E	1904651	biological_process	positive regulation of fat cell apoptotic process
E	1990051	biological_process	activation of protein kinase C activity
E	1990460	molecular_function	leptin receptor binding
E	2000379	biological_process	positive regulation of reactive oxygen species metabolic process
E	2000486	biological_process	negative regulation of glutamine transport
E	2000491	biological_process	positive regulation of hepatic stellate cell activation
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0004896	molecular_function	cytokine receptor activity
F	0006355	biological_process	regulation of DNA-templated transcription
F	0016020	cellular_component	membrane

Functional Information from PROSITE/UniProt

site_id	PS01353
Number of Residues	57
Details	HEMATOPO_REC_L_F2 Long hematopoietin receptor, gp130 family signature. NsigaSvanfnltfswpmskvnivqslsayplnsscvi...VsWilsPsdyklmy..Fi.IeW

Chain	Residue	Details
B	ASN718-TRP774

---

site_id	PS01355
Number of Residues	31
Details	HEMATOPO_REC_S_F1 Short hematopoietin receptor family 1 signature. IlpgssyevqVRgkrldgpgi...........WSdWStprvF

Chain	Residue	Details
B	ILE298-PHE328

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2451
Details	TOPO_DOM: Extracellular => ECO:0000255

Chain	Residue	Details
B	PHE22-ASP839
D	PHE22-ASP839
F	PHE22-ASP839

---

site_id	SWS_FT_FI2
Number of Residues	45
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:9786864

Chain	Residue	Details
B	ASN23
B	ASN624
B	ASN659
B	ASN688
B	ASN697
B	ASN728
B	ASN750
D	ASN23
D	ASN41
D	ASN56
D	ASN73
B	ASN41
D	ASN81
D	ASN98
D	ASN187
D	ASN206
D	ASN347
D	ASN624
D	ASN659
D	ASN688
D	ASN697
D	ASN728
B	ASN56
D	ASN750
F	ASN23
F	ASN41
F	ASN56
F	ASN73
F	ASN81
F	ASN98
F	ASN187
F	ASN206
F	ASN347
B	ASN73
F	ASN624
F	ASN659
F	ASN688
F	ASN697
F	ASN728
F	ASN750
B	ASN81
B	ASN98
B	ASN187
B	ASN206
B	ASN347

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:9786864

Chain	Residue	Details
B	ASN276
D	ASN276
F	ASN276

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:9786864

Chain	Residue	Details
B	ASN397
D	ASN397
F	ASN397

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:9786864

Chain	Residue	Details
B	ASN516
D	ASN516
F	ASN516

---