8AGC

Structure of yeast oligosaccharylransferase complex with lipid-linked oligosaccharide and non-acceptor peptide bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004576	molecular_function	oligosaccharyl transferase activity
A	0004579	molecular_function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
A	0005515	molecular_function	protein binding
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0006486	biological_process	protein glycosylation
A	0006487	biological_process	protein N-linked glycosylation
A	0008250	cellular_component	oligosaccharyltransferase complex
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0018279	biological_process	protein N-linked glycosylation via asparagine
A	0043687	biological_process	post-translational protein modification
A	0046872	molecular_function	metal ion binding
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0006486	biological_process	protein glycosylation
B	0006487	biological_process	protein N-linked glycosylation
B	0008250	cellular_component	oligosaccharyltransferase complex
B	0016020	cellular_component	membrane
B	0030674	molecular_function	protein-macromolecule adaptor activity
C	0005198	molecular_function	structural molecule activity
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0006486	biological_process	protein glycosylation
C	0006487	biological_process	protein N-linked glycosylation
C	0008250	cellular_component	oligosaccharyltransferase complex
C	0016020	cellular_component	membrane
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0006486	biological_process	protein glycosylation
D	0006487	biological_process	protein N-linked glycosylation
D	0008250	cellular_component	oligosaccharyltransferase complex
D	0016020	cellular_component	membrane
E	0006486	biological_process	protein glycosylation
E	0016020	cellular_component	membrane
F	0008250	cellular_component	oligosaccharyltransferase complex
F	0016020	cellular_component	membrane
G	0005789	cellular_component	endoplasmic reticulum membrane
G	0018279	biological_process	protein N-linked glycosylation via asparagine

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	215
Details	TOPO_DOM: Lumenal => ECO:0000305

Chain	Residue	Details
H	MET23-LEU182
H	GLN239-MET273
H	THR328-LYS350
A	MET228-SER232
A	GLY287-VAL300
A	LEU379-GLU384
A	TYR428-LYS441

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
H	PRO183-PHE203
A	HIS385-VAL401
A	MET406-ILE427
A	PRO442-PHE464
F	VAL228-ALA248
F	LEU252-LEU272
A	ALA169-THR188
A	ILE191-VAL205
A	TYR211-LEU227
A	SER233-PHE258
A	MET267-LYS286
A	TRP356-PHE378

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
H	LYS204-ARG217
H	ARG295-TYR306
A	SER206-GLY210
A	LEU259-HIS266
A	ALA323-SER355
A	MET402-LEU405
A	HIS465-VAL718

---

site_id	SWS_FT_FI4
Number of Residues	60
Details	TRANSMEM: Helical => ECO:0000269|PubMed:29301962

Chain	Residue	Details
H	ILE218-ASN238
H	VAL274-LEU294
H	PHE307-LEU327

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:29301962

Chain	Residue	Details
E	ASN336
E	ASN400
A	GLU168
A	ARG404
A	TYR521

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	SITE: Interacts with target acceptor peptide in protein substrate => ECO:0000250|UniProtKB:B9KDD4

Chain	Residue	Details
A	ASP47
A	GLU350
A	LYS586

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Important for catalytic activity => ECO:0000250|UniProtKB:B9KDD4

Chain	Residue	Details
A	ARG159

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498

Chain	Residue	Details
A	ASN60
A	ASN535

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	CARBOHYD: N-linked (GlcNAc...) (high mannose) asparagine => ECO:0000269|PubMed:29301962

Chain	Residue	Details
A	ASN539

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