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7ZR0

CryoEM structure of HSP90-CDC37-BRAF(V600E) complex.

Functional Information from GO Data
ChainGOidnamespacecontents
A0001890biological_processplacenta development
A0003723molecular_functionRNA binding
A0003725molecular_functiondouble-stranded RNA binding
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005576cellular_componentextracellular region
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005829cellular_componentcytosol
A0005886cellular_componentplasma membrane
A0006457biological_processprotein folding
A0006986biological_processresponse to unfolded protein
A0007004biological_processtelomere maintenance via telomerase
A0008180cellular_componentCOP9 signalosome
A0009986cellular_componentcell surface
A0010033biological_processobsolete response to organic substance
A0016020cellular_componentmembrane
A0016887molecular_functionATP hydrolysis activity
A0019062biological_processvirion attachment to host cell
A0019887molecular_functionprotein kinase regulator activity
A0019900molecular_functionkinase binding
A0019901molecular_functionprotein kinase binding
A0023026molecular_functionMHC class II protein complex binding
A0030235molecular_functionnitric-oxide synthase regulator activity
A0030511biological_processpositive regulation of transforming growth factor beta receptor signaling pathway
A0030911molecular_functionTPR domain binding
A0031072molecular_functionheat shock protein binding
A0031396biological_processregulation of protein ubiquitination
A0031625molecular_functionubiquitin protein ligase binding
A0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
A0032516biological_processpositive regulation of phosphoprotein phosphatase activity
A0032880biological_processregulation of protein localization
A0032991cellular_componentprotein-containing complex
A0034605biological_processcellular response to heat
A0034751cellular_componentaryl hydrocarbon receptor complex
A0034774cellular_componentsecretory granule lumen
A0042277molecular_functionpeptide binding
A0042470cellular_componentmelanosome
A0042802molecular_functionidentical protein binding
A0042803molecular_functionprotein homodimerization activity
A0042826molecular_functionhistone deacetylase binding
A0043008molecular_functionATP-dependent protein binding
A0043025cellular_componentneuronal cell body
A0043066biological_processnegative regulation of apoptotic process
A0044183molecular_functionprotein folding chaperone
A0044294cellular_componentdendritic growth cone
A0044295cellular_componentaxonal growth cone
A0045296molecular_functioncadherin binding
A0045429biological_processpositive regulation of nitric oxide biosynthetic process
A0045597biological_processpositive regulation of cell differentiation
A0046983molecular_functionprotein dimerization activity
A0048156molecular_functiontau protein binding
A0048471cellular_componentperinuclear region of cytoplasm
A0050821biological_processprotein stabilization
A0051082molecular_functionunfolded protein binding
A0051131biological_processchaperone-mediated protein complex assembly
A0051726biological_processregulation of cell cycle
A0061077biological_processchaperone-mediated protein folding
A0070062cellular_componentextracellular exosome
A0070182molecular_functionDNA polymerase binding
A0071353biological_processcellular response to interleukin-4
A0097435biological_processsupramolecular fiber organization
A0097718molecular_functiondisordered domain specific binding
A0101031cellular_componentprotein folding chaperone complex
A0120293cellular_componentdynein axonemal particle
A0140662molecular_functionATP-dependent protein folding chaperone
A0141069molecular_functionreceptor ligand inhibitor activity
A1901799biological_processnegative regulation of proteasomal protein catabolic process
A1904813cellular_componentficolin-1-rich granule lumen
A1905323biological_processtelomerase holoenzyme complex assembly
A1990226molecular_functionhistone methyltransferase binding
A1990565cellular_componentHSP90-CDC37 chaperone complex
A2000010biological_processpositive regulation of protein localization to cell surface
B0001890biological_processplacenta development
B0003723molecular_functionRNA binding
B0003725molecular_functiondouble-stranded RNA binding
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005576cellular_componentextracellular region
B0005634cellular_componentnucleus
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005829cellular_componentcytosol
B0005886cellular_componentplasma membrane
B0006457biological_processprotein folding
B0006986biological_processresponse to unfolded protein
B0007004biological_processtelomere maintenance via telomerase
B0008180cellular_componentCOP9 signalosome
B0009986cellular_componentcell surface
B0010033biological_processobsolete response to organic substance
B0016020cellular_componentmembrane
B0016887molecular_functionATP hydrolysis activity
B0019062biological_processvirion attachment to host cell
B0019887molecular_functionprotein kinase regulator activity
B0019900molecular_functionkinase binding
B0019901molecular_functionprotein kinase binding
B0023026molecular_functionMHC class II protein complex binding
B0030235molecular_functionnitric-oxide synthase regulator activity
B0030511biological_processpositive regulation of transforming growth factor beta receptor signaling pathway
B0030911molecular_functionTPR domain binding
B0031072molecular_functionheat shock protein binding
B0031396biological_processregulation of protein ubiquitination
B0031625molecular_functionubiquitin protein ligase binding
B0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
B0032516biological_processpositive regulation of phosphoprotein phosphatase activity
B0032880biological_processregulation of protein localization
B0032991cellular_componentprotein-containing complex
B0034605biological_processcellular response to heat
B0034751cellular_componentaryl hydrocarbon receptor complex
B0034774cellular_componentsecretory granule lumen
B0042277molecular_functionpeptide binding
B0042470cellular_componentmelanosome
B0042802molecular_functionidentical protein binding
B0042803molecular_functionprotein homodimerization activity
B0042826molecular_functionhistone deacetylase binding
B0043008molecular_functionATP-dependent protein binding
B0043025cellular_componentneuronal cell body
B0043066biological_processnegative regulation of apoptotic process
B0044183molecular_functionprotein folding chaperone
B0044294cellular_componentdendritic growth cone
B0044295cellular_componentaxonal growth cone
B0045296molecular_functioncadherin binding
B0045429biological_processpositive regulation of nitric oxide biosynthetic process
B0045597biological_processpositive regulation of cell differentiation
B0046983molecular_functionprotein dimerization activity
B0048156molecular_functiontau protein binding
B0048471cellular_componentperinuclear region of cytoplasm
B0050821biological_processprotein stabilization
B0051082molecular_functionunfolded protein binding
B0051131biological_processchaperone-mediated protein complex assembly
B0051726biological_processregulation of cell cycle
B0061077biological_processchaperone-mediated protein folding
B0070062cellular_componentextracellular exosome
B0070182molecular_functionDNA polymerase binding
B0071353biological_processcellular response to interleukin-4
B0097435biological_processsupramolecular fiber organization
B0097718molecular_functiondisordered domain specific binding
B0101031cellular_componentprotein folding chaperone complex
B0120293cellular_componentdynein axonemal particle
B0140662molecular_functionATP-dependent protein folding chaperone
B0141069molecular_functionreceptor ligand inhibitor activity
B1901799biological_processnegative regulation of proteasomal protein catabolic process
B1904813cellular_componentficolin-1-rich granule lumen
B1905323biological_processtelomerase holoenzyme complex assembly
B1990226molecular_functionhistone methyltransferase binding
B1990565cellular_componentHSP90-CDC37 chaperone complex
B2000010biological_processpositive regulation of protein localization to cell surface
C0000079biological_processregulation of cyclin-dependent protein serine/threonine kinase activity
C0005515molecular_functionprotein binding
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0006457biological_processprotein folding
C0006605biological_processprotein targeting
C0010608biological_processpost-transcriptional regulation of gene expression
C0019887molecular_functionprotein kinase regulator activity
C0019900molecular_functionkinase binding
C0019901molecular_functionprotein kinase binding
C0031072molecular_functionheat shock protein binding
C0050821biological_processprotein stabilization
C0051082molecular_functionunfolded protein binding
C0051087molecular_functionprotein-folding chaperone binding
C0051879molecular_functionHsp90 protein binding
C0060334biological_processregulation of type II interferon-mediated signaling pathway
C0060338biological_processregulation of type I interferon-mediated signaling pathway
C0070062cellular_componentextracellular exosome
C0097110molecular_functionscaffold protein binding
C0098779biological_processpositive regulation of mitophagy in response to mitochondrial depolarization
C0101031cellular_componentprotein folding chaperone complex
C1990565cellular_componentHSP90-CDC37 chaperone complex
K0000165biological_processMAPK cascade
K0001934biological_processpositive regulation of protein phosphorylation
K0002318biological_processmyeloid progenitor cell differentiation
K0004672molecular_functionprotein kinase activity
K0004674molecular_functionprotein serine/threonine kinase activity
K0004708molecular_functionMAP kinase kinase activity
K0004709molecular_functionMAP kinase kinase kinase activity
K0005509molecular_functioncalcium ion binding
K0005515molecular_functionprotein binding
K0005524molecular_functionATP binding
K0005634cellular_componentnucleus
K0005737cellular_componentcytoplasm
K0005739cellular_componentmitochondrion
K0005829cellular_componentcytosol
K0005886cellular_componentplasma membrane
K0006468biological_processprotein phosphorylation
K0007165biological_processsignal transduction
K0007173biological_processepidermal growth factor receptor signaling pathway
K0008542biological_processvisual learning
K0009887biological_processanimal organ morphogenesis
K0010628biological_processpositive regulation of gene expression
K0010764biological_processnegative regulation of fibroblast migration
K0010828biological_processpositive regulation of glucose transmembrane transport
K0016079biological_processsynaptic vesicle exocytosis
K0030154biological_processcell differentiation
K0030878biological_processthyroid gland development
K0031267molecular_functionsmall GTPase binding
K0031434molecular_functionmitogen-activated protein kinase kinase binding
K0033077biological_processT cell differentiation in thymus
K0033138biological_processpositive regulation of peptidyl-serine phosphorylation
K0034446biological_processsubstrate adhesion-dependent cell spreading
K0035019biological_processsomatic stem cell population maintenance
K0036211biological_processprotein modification process
K0042127biological_processregulation of cell population proliferation
K0042802molecular_functionidentical protein binding
K0043005cellular_componentneuron projection
K0043066biological_processnegative regulation of apoptotic process
K0043149biological_processstress fiber assembly
K0043231cellular_componentintracellular membrane-bounded organelle
K0043367biological_processCD4-positive, alpha-beta T cell differentiation
K0043368biological_processpositive T cell selection
K0043369biological_processCD4-positive or CD8-positive, alpha-beta T cell lineage commitment
K0043434biological_processresponse to peptide hormone
K0043524biological_processnegative regulation of neuron apoptotic process
K0044297cellular_componentcell body
K0044877molecular_functionprotein-containing complex binding
K0045580biological_processregulation of T cell differentiation
K0046632biological_processalpha-beta T cell differentiation
K0046872molecular_functionmetal ion binding
K0048538biological_processthymus development
K0048679biological_processregulation of axon regeneration
K0048680biological_processpositive regulation of axon regeneration
K0050772biological_processpositive regulation of axonogenesis
K0050852biological_processT cell receptor signaling pathway
K0051496biological_processpositive regulation of stress fiber assembly
K0051591biological_processresponse to cAMP
K0060291biological_processlong-term synaptic potentiation
K0060323biological_processhead morphogenesis
K0060324biological_processface development
K0070371biological_processERK1 and ERK2 cascade
K0070374biological_processpositive regulation of ERK1 and ERK2 cascade
K0071277biological_processcellular response to calcium ion
K0071466biological_processcellular response to xenobiotic stimulus
K0072577biological_processendothelial cell apoptotic process
K0090150biological_processestablishment of protein localization to membrane
K0097110molecular_functionscaffold protein binding
K0098793cellular_componentpresynapse
K0106310molecular_functionprotein serine kinase activity
K1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
K1902531biological_processregulation of intracellular signal transduction
K1990090biological_processcellular response to nerve growth factor stimulus
K2000301biological_processnegative regulation of synaptic vesicle exocytosis
K2000352biological_processnegative regulation of endothelial cell apoptotic process
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues21
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGSGSFGTVYkGkwhgd.............VAVK
ChainResidueDetails
KILE463-LYS483

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiHrDLKsnNIFL
ChainResidueDetails
KILE572-LEU584

site_idPS00298
Number of Residues10
DetailsHSP90 Heat shock hsp90 proteins family signature. YsNKEIFLRE
ChainResidueDetails
ATYR33-GLU42

site_idPS00479
Number of Residues46
DetailsZF_DAG_PE_1 Zinc finger phorbol-ester/DAG-type signature. HnFvrktfftlaf.CdfCrklLfqgfr.....CqtCgykfHqrCstevplm..C
ChainResidueDetails
KHIS235-CYS280

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues46
DetailsZN_FING: Phorbol-ester/DAG-type => ECO:0000255|PROSITE-ProRule:PRU00226
ChainResidueDetails
KTHR234-CYS280
ALYS107
APHE133
AARG392
BASN46
BLYS107
BPHE133
BARG392

site_idSWS_FT_FI2
Number of Residues1
DetailsACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027
ChainResidueDetails
KASP576
BASP88

site_idSWS_FT_FI3
Number of Residues8
DetailsBINDING: BINDING => ECO:0000250
ChainResidueDetails
KHIS235
KCYS248
KCYS251
KCYS261
KCYS264
KHIS269
KCYS272
KCYS280

site_idSWS_FT_FI4
Number of Residues2
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159
ChainResidueDetails
KILE463
KLYS483
BLYS219
BLYS577

site_idSWS_FT_FI5
Number of Residues2
DetailsSITE: Breakpoint for translocation to form KIAA1549-BRAF fusion protein
ChainResidueDetails
KASP380
KMET438

site_idSWS_FT_FI6
Number of Residues1
DetailsMOD_RES: N-acetylalanine => ECO:0000269|Ref.8
ChainResidueDetails
KALA2
BSER255

site_idSWS_FT_FI7
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569
ChainResidueDetails
KSER151
BSER261

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P28028
ChainResidueDetails
KSER333
KSER750

site_idSWS_FT_FI9
Number of Residues1
DetailsMOD_RES: Phosphoserine; by SGK1 => ECO:0000269|PubMed:11410590, ECO:0000269|Ref.8, ECO:0007744|PubMed:23186163
ChainResidueDetails
KSER365
BTYR301

site_idSWS_FT_FI10
Number of Residues1
DetailsMOD_RES: Phosphothreonine; by autocatalysis => ECO:0000269|PubMed:1508179
ChainResidueDetails
KTHR373
ATYR484
BTYR305
BTYR484

site_idSWS_FT_FI11
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000269|Ref.8
ChainResidueDetails
KTHR396
BSER307

site_idSWS_FT_FI12
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000269|Ref.8
ChainResidueDetails
KSER399
BLYS399

site_idSWS_FT_FI13
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000269|Ref.8, ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
KTHR401
ALYS481
BLYS435
BLYS481

site_idSWS_FT_FI14
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
KSER446
BSER445

site_idSWS_FT_FI15
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648
ChainResidueDetails
KSER447
BTHR479

site_idSWS_FT_FI16
Number of Residues1
DetailsMOD_RES: Omega-N-methylarginine; by PRMT5 => ECO:0000269|PubMed:21917714
ChainResidueDetails
KARG671
BLYS531

site_idSWS_FT_FI17
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000269|Ref.8, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163
ChainResidueDetails
KSER729
BLYS574

site_idSWS_FT_FI18
Number of Residues1
DetailsMOD_RES: Phosphothreonine; by MAPK1 => ECO:0000269|PubMed:19710016
ChainResidueDetails
KTHR753
BCYS590

site_idSWS_FT_FI19
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:23907581
ChainResidueDetails
KLYS578
BLYS624

site_idSWS_FT_FI20
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569
ChainResidueDetails
ASER669
BSER669

site_idSWS_FT_FI21
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PLK2 and PLK3 => ECO:0000269|PubMed:22828320
ChainResidueDetails
ASER718
BSER718

site_idSWS_FT_FI22
Number of Residues4
DetailsCARBOHYD: O-linked (GlcNAc) serine => ECO:0000250
ChainResidueDetails
ASER434
ASER452
BSER434
BSER452

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PDB entries from 2024-07-24

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