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7YIV

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (ALPL) at Basic pH

Functional Information from GO Data
ChainGOidnamespacecontents
A0001501biological_processskeletal system development
A0001649biological_processosteoblast differentiation
A0001958biological_processendochondral ossification
A0003006biological_processdevelopmental process involved in reproduction
A0004035molecular_functionalkaline phosphatase activity
A0004427molecular_functioninorganic diphosphate phosphatase activity
A0005509molecular_functioncalcium ion binding
A0005576cellular_componentextracellular region
A0005615cellular_componentextracellular space
A0005739cellular_componentmitochondrion
A0005758cellular_componentmitochondrial intermembrane space
A0005886cellular_componentplasma membrane
A0014070biological_processresponse to organic cyclic compound
A0016020cellular_componentmembrane
A0016462molecular_functionpyrophosphatase activity
A0016787molecular_functionhydrolase activity
A0016791molecular_functionphosphatase activity
A0016887molecular_functionATP hydrolysis activity
A0019725biological_processcellular homeostasis
A0030282biological_processbone mineralization
A0031012cellular_componentextracellular matrix
A0031214biological_processbiomineral tissue development
A0031966cellular_componentmitochondrial membrane
A0032496biological_processresponse to lipopolysaccharide
A0032868biological_processresponse to insulin
A0033280biological_processresponse to vitamin D
A0033883molecular_functionpyridoxal phosphatase activity
A0034516biological_processresponse to vitamin B6
A0036005biological_processresponse to macrophage colony-stimulating factor
A0042822biological_processpyridoxal phosphate metabolic process
A0043262molecular_functionADP phosphatase activity
A0046677biological_processresponse to antibiotic
A0046872molecular_functionmetal ion binding
A0050187molecular_functionphosphoamidase activity
A0051384biological_processresponse to glucocorticoid
A0052732molecular_functionphosphoethanolamine phosphatase activity
A0055062biological_processphosphate ion homeostasis
A0055074biological_processcalcium ion homeostasis
A0065010cellular_componentextracellular membrane-bounded organelle
A0070062cellular_componentextracellular exosome
A0071407biological_processcellular response to organic cyclic compound
A0071529biological_processcementum mineralization
A0098552cellular_componentside of membrane
A0120162biological_processpositive regulation of cold-induced thermogenesis
A0140651biological_processfutile creatine cycle
A0140928biological_processinhibition of non-skeletal tissue mineralization
A1904383biological_processresponse to sodium phosphate
B0001501biological_processskeletal system development
B0001649biological_processosteoblast differentiation
B0001958biological_processendochondral ossification
B0003006biological_processdevelopmental process involved in reproduction
B0004035molecular_functionalkaline phosphatase activity
B0004427molecular_functioninorganic diphosphate phosphatase activity
B0005509molecular_functioncalcium ion binding
B0005576cellular_componentextracellular region
B0005615cellular_componentextracellular space
B0005739cellular_componentmitochondrion
B0005758cellular_componentmitochondrial intermembrane space
B0005886cellular_componentplasma membrane
B0014070biological_processresponse to organic cyclic compound
B0016020cellular_componentmembrane
B0016462molecular_functionpyrophosphatase activity
B0016787molecular_functionhydrolase activity
B0016791molecular_functionphosphatase activity
B0016887molecular_functionATP hydrolysis activity
B0019725biological_processcellular homeostasis
B0030282biological_processbone mineralization
B0031012cellular_componentextracellular matrix
B0031214biological_processbiomineral tissue development
B0031966cellular_componentmitochondrial membrane
B0032496biological_processresponse to lipopolysaccharide
B0032868biological_processresponse to insulin
B0033280biological_processresponse to vitamin D
B0033883molecular_functionpyridoxal phosphatase activity
B0034516biological_processresponse to vitamin B6
B0036005biological_processresponse to macrophage colony-stimulating factor
B0042822biological_processpyridoxal phosphate metabolic process
B0043262molecular_functionADP phosphatase activity
B0046677biological_processresponse to antibiotic
B0046872molecular_functionmetal ion binding
B0050187molecular_functionphosphoamidase activity
B0051384biological_processresponse to glucocorticoid
B0052732molecular_functionphosphoethanolamine phosphatase activity
B0055062biological_processphosphate ion homeostasis
B0055074biological_processcalcium ion homeostasis
B0065010cellular_componentextracellular membrane-bounded organelle
B0070062cellular_componentextracellular exosome
B0071407biological_processcellular response to organic cyclic compound
B0071529biological_processcementum mineralization
B0098552cellular_componentside of membrane
B0120162biological_processpositive regulation of cold-induced thermogenesis
B0140651biological_processfutile creatine cycle
B0140928biological_processinhibition of non-skeletal tissue mineralization
B1904383biological_processresponse to sodium phosphate
C0001501biological_processskeletal system development
C0001649biological_processosteoblast differentiation
C0001958biological_processendochondral ossification
C0003006biological_processdevelopmental process involved in reproduction
C0004035molecular_functionalkaline phosphatase activity
C0004427molecular_functioninorganic diphosphate phosphatase activity
C0005509molecular_functioncalcium ion binding
C0005576cellular_componentextracellular region
C0005615cellular_componentextracellular space
C0005739cellular_componentmitochondrion
C0005758cellular_componentmitochondrial intermembrane space
C0005886cellular_componentplasma membrane
C0014070biological_processresponse to organic cyclic compound
C0016020cellular_componentmembrane
C0016462molecular_functionpyrophosphatase activity
C0016787molecular_functionhydrolase activity
C0016791molecular_functionphosphatase activity
C0016887molecular_functionATP hydrolysis activity
C0019725biological_processcellular homeostasis
C0030282biological_processbone mineralization
C0031012cellular_componentextracellular matrix
C0031214biological_processbiomineral tissue development
C0031966cellular_componentmitochondrial membrane
C0032496biological_processresponse to lipopolysaccharide
C0032868biological_processresponse to insulin
C0033280biological_processresponse to vitamin D
C0033883molecular_functionpyridoxal phosphatase activity
C0034516biological_processresponse to vitamin B6
C0036005biological_processresponse to macrophage colony-stimulating factor
C0042822biological_processpyridoxal phosphate metabolic process
C0043262molecular_functionADP phosphatase activity
C0046677biological_processresponse to antibiotic
C0046872molecular_functionmetal ion binding
C0050187molecular_functionphosphoamidase activity
C0051384biological_processresponse to glucocorticoid
C0052732molecular_functionphosphoethanolamine phosphatase activity
C0055062biological_processphosphate ion homeostasis
C0055074biological_processcalcium ion homeostasis
C0065010cellular_componentextracellular membrane-bounded organelle
C0070062cellular_componentextracellular exosome
C0071407biological_processcellular response to organic cyclic compound
C0071529biological_processcementum mineralization
C0098552cellular_componentside of membrane
C0120162biological_processpositive regulation of cold-induced thermogenesis
C0140651biological_processfutile creatine cycle
C0140928biological_processinhibition of non-skeletal tissue mineralization
C1904383biological_processresponse to sodium phosphate
D0001501biological_processskeletal system development
D0001649biological_processosteoblast differentiation
D0001958biological_processendochondral ossification
D0003006biological_processdevelopmental process involved in reproduction
D0004035molecular_functionalkaline phosphatase activity
D0004427molecular_functioninorganic diphosphate phosphatase activity
D0005509molecular_functioncalcium ion binding
D0005576cellular_componentextracellular region
D0005615cellular_componentextracellular space
D0005739cellular_componentmitochondrion
D0005758cellular_componentmitochondrial intermembrane space
D0005886cellular_componentplasma membrane
D0014070biological_processresponse to organic cyclic compound
D0016020cellular_componentmembrane
D0016462molecular_functionpyrophosphatase activity
D0016787molecular_functionhydrolase activity
D0016791molecular_functionphosphatase activity
D0016887molecular_functionATP hydrolysis activity
D0019725biological_processcellular homeostasis
D0030282biological_processbone mineralization
D0031012cellular_componentextracellular matrix
D0031214biological_processbiomineral tissue development
D0031966cellular_componentmitochondrial membrane
D0032496biological_processresponse to lipopolysaccharide
D0032868biological_processresponse to insulin
D0033280biological_processresponse to vitamin D
D0033883molecular_functionpyridoxal phosphatase activity
D0034516biological_processresponse to vitamin B6
D0036005biological_processresponse to macrophage colony-stimulating factor
D0042822biological_processpyridoxal phosphate metabolic process
D0043262molecular_functionADP phosphatase activity
D0046677biological_processresponse to antibiotic
D0046872molecular_functionmetal ion binding
D0050187molecular_functionphosphoamidase activity
D0051384biological_processresponse to glucocorticoid
D0052732molecular_functionphosphoethanolamine phosphatase activity
D0055062biological_processphosphate ion homeostasis
D0055074biological_processcalcium ion homeostasis
D0065010cellular_componentextracellular membrane-bounded organelle
D0070062cellular_componentextracellular exosome
D0071407biological_processcellular response to organic cyclic compound
D0071529biological_processcementum mineralization
D0098552cellular_componentside of membrane
D0120162biological_processpositive regulation of cold-induced thermogenesis
D0140651biological_processfutile creatine cycle
D0140928biological_processinhibition of non-skeletal tissue mineralization
D1904383biological_processresponse to sodium phosphate
E0001501biological_processskeletal system development
E0001649biological_processosteoblast differentiation
E0001958biological_processendochondral ossification
E0003006biological_processdevelopmental process involved in reproduction
E0004035molecular_functionalkaline phosphatase activity
E0004427molecular_functioninorganic diphosphate phosphatase activity
E0005509molecular_functioncalcium ion binding
E0005576cellular_componentextracellular region
E0005615cellular_componentextracellular space
E0005739cellular_componentmitochondrion
E0005758cellular_componentmitochondrial intermembrane space
E0005886cellular_componentplasma membrane
E0014070biological_processresponse to organic cyclic compound
E0016020cellular_componentmembrane
E0016462molecular_functionpyrophosphatase activity
E0016787molecular_functionhydrolase activity
E0016791molecular_functionphosphatase activity
E0016887molecular_functionATP hydrolysis activity
E0019725biological_processcellular homeostasis
E0030282biological_processbone mineralization
E0031012cellular_componentextracellular matrix
E0031214biological_processbiomineral tissue development
E0031966cellular_componentmitochondrial membrane
E0032496biological_processresponse to lipopolysaccharide
E0032868biological_processresponse to insulin
E0033280biological_processresponse to vitamin D
E0033883molecular_functionpyridoxal phosphatase activity
E0034516biological_processresponse to vitamin B6
E0036005biological_processresponse to macrophage colony-stimulating factor
E0042822biological_processpyridoxal phosphate metabolic process
E0043262molecular_functionADP phosphatase activity
E0046677biological_processresponse to antibiotic
E0046872molecular_functionmetal ion binding
E0050187molecular_functionphosphoamidase activity
E0051384biological_processresponse to glucocorticoid
E0052732molecular_functionphosphoethanolamine phosphatase activity
E0055062biological_processphosphate ion homeostasis
E0055074biological_processcalcium ion homeostasis
E0065010cellular_componentextracellular membrane-bounded organelle
E0070062cellular_componentextracellular exosome
E0071407biological_processcellular response to organic cyclic compound
E0071529biological_processcementum mineralization
E0098552cellular_componentside of membrane
E0120162biological_processpositive regulation of cold-induced thermogenesis
E0140651biological_processfutile creatine cycle
E0140928biological_processinhibition of non-skeletal tissue mineralization
E1904383biological_processresponse to sodium phosphate
F0001501biological_processskeletal system development
F0001649biological_processosteoblast differentiation
F0001958biological_processendochondral ossification
F0003006biological_processdevelopmental process involved in reproduction
F0004035molecular_functionalkaline phosphatase activity
F0004427molecular_functioninorganic diphosphate phosphatase activity
F0005509molecular_functioncalcium ion binding
F0005576cellular_componentextracellular region
F0005615cellular_componentextracellular space
F0005739cellular_componentmitochondrion
F0005758cellular_componentmitochondrial intermembrane space
F0005886cellular_componentplasma membrane
F0014070biological_processresponse to organic cyclic compound
F0016020cellular_componentmembrane
F0016462molecular_functionpyrophosphatase activity
F0016787molecular_functionhydrolase activity
F0016791molecular_functionphosphatase activity
F0016887molecular_functionATP hydrolysis activity
F0019725biological_processcellular homeostasis
F0030282biological_processbone mineralization
F0031012cellular_componentextracellular matrix
F0031214biological_processbiomineral tissue development
F0031966cellular_componentmitochondrial membrane
F0032496biological_processresponse to lipopolysaccharide
F0032868biological_processresponse to insulin
F0033280biological_processresponse to vitamin D
F0033883molecular_functionpyridoxal phosphatase activity
F0034516biological_processresponse to vitamin B6
F0036005biological_processresponse to macrophage colony-stimulating factor
F0042822biological_processpyridoxal phosphate metabolic process
F0043262molecular_functionADP phosphatase activity
F0046677biological_processresponse to antibiotic
F0046872molecular_functionmetal ion binding
F0050187molecular_functionphosphoamidase activity
F0051384biological_processresponse to glucocorticoid
F0052732molecular_functionphosphoethanolamine phosphatase activity
F0055062biological_processphosphate ion homeostasis
F0055074biological_processcalcium ion homeostasis
F0065010cellular_componentextracellular membrane-bounded organelle
F0070062cellular_componentextracellular exosome
F0071407biological_processcellular response to organic cyclic compound
F0071529biological_processcementum mineralization
F0098552cellular_componentside of membrane
F0120162biological_processpositive regulation of cold-induced thermogenesis
F0140651biological_processfutile creatine cycle
F0140928biological_processinhibition of non-skeletal tissue mineralization
F1904383biological_processresponse to sodium phosphate
G0001501biological_processskeletal system development
G0001649biological_processosteoblast differentiation
G0001958biological_processendochondral ossification
G0003006biological_processdevelopmental process involved in reproduction
G0004035molecular_functionalkaline phosphatase activity
G0004427molecular_functioninorganic diphosphate phosphatase activity
G0005509molecular_functioncalcium ion binding
G0005576cellular_componentextracellular region
G0005615cellular_componentextracellular space
G0005739cellular_componentmitochondrion
G0005758cellular_componentmitochondrial intermembrane space
G0005886cellular_componentplasma membrane
G0014070biological_processresponse to organic cyclic compound
G0016020cellular_componentmembrane
G0016462molecular_functionpyrophosphatase activity
G0016787molecular_functionhydrolase activity
G0016791molecular_functionphosphatase activity
G0016887molecular_functionATP hydrolysis activity
G0019725biological_processcellular homeostasis
G0030282biological_processbone mineralization
G0031012cellular_componentextracellular matrix
G0031214biological_processbiomineral tissue development
G0031966cellular_componentmitochondrial membrane
G0032496biological_processresponse to lipopolysaccharide
G0032868biological_processresponse to insulin
G0033280biological_processresponse to vitamin D
G0033883molecular_functionpyridoxal phosphatase activity
G0034516biological_processresponse to vitamin B6
G0036005biological_processresponse to macrophage colony-stimulating factor
G0042822biological_processpyridoxal phosphate metabolic process
G0043262molecular_functionADP phosphatase activity
G0046677biological_processresponse to antibiotic
G0046872molecular_functionmetal ion binding
G0050187molecular_functionphosphoamidase activity
G0051384biological_processresponse to glucocorticoid
G0052732molecular_functionphosphoethanolamine phosphatase activity
G0055062biological_processphosphate ion homeostasis
G0055074biological_processcalcium ion homeostasis
G0065010cellular_componentextracellular membrane-bounded organelle
G0070062cellular_componentextracellular exosome
G0071407biological_processcellular response to organic cyclic compound
G0071529biological_processcementum mineralization
G0098552cellular_componentside of membrane
G0120162biological_processpositive regulation of cold-induced thermogenesis
G0140651biological_processfutile creatine cycle
G0140928biological_processinhibition of non-skeletal tissue mineralization
G1904383biological_processresponse to sodium phosphate
H0001501biological_processskeletal system development
H0001649biological_processosteoblast differentiation
H0001958biological_processendochondral ossification
H0003006biological_processdevelopmental process involved in reproduction
H0004035molecular_functionalkaline phosphatase activity
H0004427molecular_functioninorganic diphosphate phosphatase activity
H0005509molecular_functioncalcium ion binding
H0005576cellular_componentextracellular region
H0005615cellular_componentextracellular space
H0005739cellular_componentmitochondrion
H0005758cellular_componentmitochondrial intermembrane space
H0005886cellular_componentplasma membrane
H0014070biological_processresponse to organic cyclic compound
H0016020cellular_componentmembrane
H0016462molecular_functionpyrophosphatase activity
H0016787molecular_functionhydrolase activity
H0016791molecular_functionphosphatase activity
H0016887molecular_functionATP hydrolysis activity
H0019725biological_processcellular homeostasis
H0030282biological_processbone mineralization
H0031012cellular_componentextracellular matrix
H0031214biological_processbiomineral tissue development
H0031966cellular_componentmitochondrial membrane
H0032496biological_processresponse to lipopolysaccharide
H0032868biological_processresponse to insulin
H0033280biological_processresponse to vitamin D
H0033883molecular_functionpyridoxal phosphatase activity
H0034516biological_processresponse to vitamin B6
H0036005biological_processresponse to macrophage colony-stimulating factor
H0042822biological_processpyridoxal phosphate metabolic process
H0043262molecular_functionADP phosphatase activity
H0046677biological_processresponse to antibiotic
H0046872molecular_functionmetal ion binding
H0050187molecular_functionphosphoamidase activity
H0051384biological_processresponse to glucocorticoid
H0052732molecular_functionphosphoethanolamine phosphatase activity
H0055062biological_processphosphate ion homeostasis
H0055074biological_processcalcium ion homeostasis
H0065010cellular_componentextracellular membrane-bounded organelle
H0070062cellular_componentextracellular exosome
H0071407biological_processcellular response to organic cyclic compound
H0071529biological_processcementum mineralization
H0098552cellular_componentside of membrane
H0120162biological_processpositive regulation of cold-induced thermogenesis
H0140651biological_processfutile creatine cycle
H0140928biological_processinhibition of non-skeletal tissue mineralization
H1904383biological_processresponse to sodium phosphate
Functional Information from PROSITE/UniProt
site_idPS00123
Number of Residues9
DetailsALKALINE_PHOSPHATASE Alkaline phosphatase active site. VpDSAGTAT
ChainResidueDetails
AVAL107-THR115

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues8
DetailsACT_SITE: Phosphoserine intermediate => ECO:0000250|UniProtKB:P05187, ECO:0000255|PROSITE-ProRule:PRU10042
ChainResidueDetails
ASER110
DSER110
CSER110
BSER110
GSER110
HSER110
FSER110
ESER110

site_idSWS_FT_FI2
Number of Residues72
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P05187
ChainResidueDetails
AASP60
DASP60
DSER110
DTHR173
DGLU332
DASP337
DHIS341
DASP378
DHIS379
DHIS454
CASP60
ASER110
CSER110
CTHR173
CGLU332
CASP337
CHIS341
CASP378
CHIS379
CHIS454
BASP60
BSER110
ATHR173
BTHR173
BGLU332
BASP337
BHIS341
BASP378
BHIS379
BHIS454
GASP60
GSER110
GTHR173
AGLU332
GGLU332
GASP337
GHIS341
GASP378
GHIS379
GHIS454
HASP60
HSER110
HTHR173
HGLU332
AASP337
HASP337
HHIS341
HASP378
HHIS379
HHIS454
FASP60
FSER110
FTHR173
FGLU332
FASP337
AHIS341
FHIS341
FASP378
FHIS379
FHIS454
EASP60
ESER110
ETHR173
EGLU332
EASP337
EHIS341
AASP378
EASP378
EHIS379
EHIS454
AHIS379
AHIS454

site_idSWS_FT_FI3
Number of Residues32
DetailsBINDING: BINDING => ECO:0000269|PubMed:11395499
ChainResidueDetails
AGLU235
CPHE290
CGLU291
CASP306
BGLU235
BPHE290
BGLU291
BASP306
GGLU235
GPHE290
GGLU291
APHE290
GASP306
HGLU235
HPHE290
HGLU291
HASP306
FGLU235
FPHE290
FGLU291
FASP306
EGLU235
AGLU291
EPHE290
EGLU291
EASP306
AASP306
DGLU235
DPHE290
DGLU291
DASP306
CGLU235

site_idSWS_FT_FI4
Number of Residues8
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P09242
ChainResidueDetails
ASER110
DSER110
CSER110
BSER110
GSER110
HSER110
FSER110
ESER110

site_idSWS_FT_FI5
Number of Residues32
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN140
CASN230
CASN271
CASN303
BASN140
BASN230
BASN271
BASN303
GASN140
GASN230
GASN271
AASN230
GASN303
HASN140
HASN230
HASN271
HASN303
FASN140
FASN230
FASN271
FASN303
EASN140
AASN271
EASN230
EASN271
EASN303
AASN303
DASN140
DASN230
DASN271
DASN303
CASN140

site_idSWS_FT_FI6
Number of Residues8
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19159218
ChainResidueDetails
AASN430
DASN430
CASN430
BASN430
GASN430
HASN430
FASN430
EASN430

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