7YIV

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (ALPL) at Basic pH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001501	biological_process	skeletal system development
A	0001649	biological_process	osteoblast differentiation
A	0004035	molecular_function	alkaline phosphatase activity
A	0004427	molecular_function	inorganic diphosphate phosphatase activity
A	0005509	molecular_function	calcium ion binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005758	cellular_component	mitochondrial intermembrane space
A	0005886	cellular_component	plasma membrane
A	0016020	cellular_component	membrane
A	0016462	molecular_function	pyrophosphatase activity
A	0016787	molecular_function	hydrolase activity
A	0016791	molecular_function	phosphatase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0030282	biological_process	bone mineralization
A	0031012	cellular_component	extracellular matrix
A	0031214	biological_process	biomineral tissue development
A	0031966	cellular_component	mitochondrial membrane
A	0032496	biological_process	response to lipopolysaccharide
A	0032868	biological_process	response to insulin
A	0033280	biological_process	response to vitamin D
A	0033883	molecular_function	pyridoxal phosphatase activity
A	0034516	biological_process	response to vitamin B6
A	0036005	biological_process	response to macrophage colony-stimulating factor
A	0042822	biological_process	pyridoxal phosphate metabolic process
A	0043262	molecular_function	ADP phosphatase activity
A	0046872	molecular_function	metal ion binding
A	0050187	molecular_function	phosphoamidase activity
A	0051384	biological_process	response to glucocorticoid
A	0052732	molecular_function	phosphoethanolamine phosphatase activity
A	0055062	biological_process	phosphate ion homeostasis
A	0055074	biological_process	calcium ion homeostasis
A	0065010	cellular_component	extracellular membrane-bounded organelle
A	0070062	cellular_component	extracellular exosome
A	0071529	biological_process	cementum mineralization
A	0098552	cellular_component	side of membrane
A	0120162	biological_process	positive regulation of cold-induced thermogenesis
A	0140651	biological_process	futile creatine cycle
A	0140928	biological_process	inhibition of non-skeletal tissue mineralization
A	1904383	biological_process	response to sodium phosphate
B	0001501	biological_process	skeletal system development
B	0001649	biological_process	osteoblast differentiation
B	0004035	molecular_function	alkaline phosphatase activity
B	0004427	molecular_function	inorganic diphosphate phosphatase activity
B	0005509	molecular_function	calcium ion binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005758	cellular_component	mitochondrial intermembrane space
B	0005886	cellular_component	plasma membrane
B	0016020	cellular_component	membrane
B	0016462	molecular_function	pyrophosphatase activity
B	0016787	molecular_function	hydrolase activity
B	0016791	molecular_function	phosphatase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0030282	biological_process	bone mineralization
B	0031012	cellular_component	extracellular matrix
B	0031214	biological_process	biomineral tissue development
B	0031966	cellular_component	mitochondrial membrane
B	0032496	biological_process	response to lipopolysaccharide
B	0032868	biological_process	response to insulin
B	0033280	biological_process	response to vitamin D
B	0033883	molecular_function	pyridoxal phosphatase activity
B	0034516	biological_process	response to vitamin B6
B	0036005	biological_process	response to macrophage colony-stimulating factor
B	0042822	biological_process	pyridoxal phosphate metabolic process
B	0043262	molecular_function	ADP phosphatase activity
B	0046872	molecular_function	metal ion binding
B	0050187	molecular_function	phosphoamidase activity
B	0051384	biological_process	response to glucocorticoid
B	0052732	molecular_function	phosphoethanolamine phosphatase activity
B	0055062	biological_process	phosphate ion homeostasis
B	0055074	biological_process	calcium ion homeostasis
B	0065010	cellular_component	extracellular membrane-bounded organelle
B	0070062	cellular_component	extracellular exosome
B	0071529	biological_process	cementum mineralization
B	0098552	cellular_component	side of membrane
B	0120162	biological_process	positive regulation of cold-induced thermogenesis
B	0140651	biological_process	futile creatine cycle
B	0140928	biological_process	inhibition of non-skeletal tissue mineralization
B	1904383	biological_process	response to sodium phosphate
C	0001501	biological_process	skeletal system development
C	0001649	biological_process	osteoblast differentiation
C	0004035	molecular_function	alkaline phosphatase activity
C	0004427	molecular_function	inorganic diphosphate phosphatase activity
C	0005509	molecular_function	calcium ion binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005758	cellular_component	mitochondrial intermembrane space
C	0005886	cellular_component	plasma membrane
C	0016020	cellular_component	membrane
C	0016462	molecular_function	pyrophosphatase activity
C	0016787	molecular_function	hydrolase activity
C	0016791	molecular_function	phosphatase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0030282	biological_process	bone mineralization
C	0031012	cellular_component	extracellular matrix
C	0031214	biological_process	biomineral tissue development
C	0031966	cellular_component	mitochondrial membrane
C	0032496	biological_process	response to lipopolysaccharide
C	0032868	biological_process	response to insulin
C	0033280	biological_process	response to vitamin D
C	0033883	molecular_function	pyridoxal phosphatase activity
C	0034516	biological_process	response to vitamin B6
C	0036005	biological_process	response to macrophage colony-stimulating factor
C	0042822	biological_process	pyridoxal phosphate metabolic process
C	0043262	molecular_function	ADP phosphatase activity
C	0046872	molecular_function	metal ion binding
C	0050187	molecular_function	phosphoamidase activity
C	0051384	biological_process	response to glucocorticoid
C	0052732	molecular_function	phosphoethanolamine phosphatase activity
C	0055062	biological_process	phosphate ion homeostasis
C	0055074	biological_process	calcium ion homeostasis
C	0065010	cellular_component	extracellular membrane-bounded organelle
C	0070062	cellular_component	extracellular exosome
C	0071529	biological_process	cementum mineralization
C	0098552	cellular_component	side of membrane
C	0120162	biological_process	positive regulation of cold-induced thermogenesis
C	0140651	biological_process	futile creatine cycle
C	0140928	biological_process	inhibition of non-skeletal tissue mineralization
C	1904383	biological_process	response to sodium phosphate
D	0001501	biological_process	skeletal system development
D	0001649	biological_process	osteoblast differentiation
D	0004035	molecular_function	alkaline phosphatase activity
D	0004427	molecular_function	inorganic diphosphate phosphatase activity
D	0005509	molecular_function	calcium ion binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005758	cellular_component	mitochondrial intermembrane space
D	0005886	cellular_component	plasma membrane
D	0016020	cellular_component	membrane
D	0016462	molecular_function	pyrophosphatase activity
D	0016787	molecular_function	hydrolase activity
D	0016791	molecular_function	phosphatase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0030282	biological_process	bone mineralization
D	0031012	cellular_component	extracellular matrix
D	0031214	biological_process	biomineral tissue development
D	0031966	cellular_component	mitochondrial membrane
D	0032496	biological_process	response to lipopolysaccharide
D	0032868	biological_process	response to insulin
D	0033280	biological_process	response to vitamin D
D	0033883	molecular_function	pyridoxal phosphatase activity
D	0034516	biological_process	response to vitamin B6
D	0036005	biological_process	response to macrophage colony-stimulating factor
D	0042822	biological_process	pyridoxal phosphate metabolic process
D	0043262	molecular_function	ADP phosphatase activity
D	0046872	molecular_function	metal ion binding
D	0050187	molecular_function	phosphoamidase activity
D	0051384	biological_process	response to glucocorticoid
D	0052732	molecular_function	phosphoethanolamine phosphatase activity
D	0055062	biological_process	phosphate ion homeostasis
D	0055074	biological_process	calcium ion homeostasis
D	0065010	cellular_component	extracellular membrane-bounded organelle
D	0070062	cellular_component	extracellular exosome
D	0071529	biological_process	cementum mineralization
D	0098552	cellular_component	side of membrane
D	0120162	biological_process	positive regulation of cold-induced thermogenesis
D	0140651	biological_process	futile creatine cycle
D	0140928	biological_process	inhibition of non-skeletal tissue mineralization
D	1904383	biological_process	response to sodium phosphate
E	0001501	biological_process	skeletal system development
E	0001649	biological_process	osteoblast differentiation
E	0004035	molecular_function	alkaline phosphatase activity
E	0004427	molecular_function	inorganic diphosphate phosphatase activity
E	0005509	molecular_function	calcium ion binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005758	cellular_component	mitochondrial intermembrane space
E	0005886	cellular_component	plasma membrane
E	0016020	cellular_component	membrane
E	0016462	molecular_function	pyrophosphatase activity
E	0016787	molecular_function	hydrolase activity
E	0016791	molecular_function	phosphatase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0030282	biological_process	bone mineralization
E	0031012	cellular_component	extracellular matrix
E	0031214	biological_process	biomineral tissue development
E	0031966	cellular_component	mitochondrial membrane
E	0032496	biological_process	response to lipopolysaccharide
E	0032868	biological_process	response to insulin
E	0033280	biological_process	response to vitamin D
E	0033883	molecular_function	pyridoxal phosphatase activity
E	0034516	biological_process	response to vitamin B6
E	0036005	biological_process	response to macrophage colony-stimulating factor
E	0042822	biological_process	pyridoxal phosphate metabolic process
E	0043262	molecular_function	ADP phosphatase activity
E	0046872	molecular_function	metal ion binding
E	0050187	molecular_function	phosphoamidase activity
E	0051384	biological_process	response to glucocorticoid
E	0052732	molecular_function	phosphoethanolamine phosphatase activity
E	0055062	biological_process	phosphate ion homeostasis
E	0055074	biological_process	calcium ion homeostasis
E	0065010	cellular_component	extracellular membrane-bounded organelle
E	0070062	cellular_component	extracellular exosome
E	0071529	biological_process	cementum mineralization
E	0098552	cellular_component	side of membrane
E	0120162	biological_process	positive regulation of cold-induced thermogenesis
E	0140651	biological_process	futile creatine cycle
E	0140928	biological_process	inhibition of non-skeletal tissue mineralization
E	1904383	biological_process	response to sodium phosphate
F	0001501	biological_process	skeletal system development
F	0001649	biological_process	osteoblast differentiation
F	0004035	molecular_function	alkaline phosphatase activity
F	0004427	molecular_function	inorganic diphosphate phosphatase activity
F	0005509	molecular_function	calcium ion binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0005758	cellular_component	mitochondrial intermembrane space
F	0005886	cellular_component	plasma membrane
F	0016020	cellular_component	membrane
F	0016462	molecular_function	pyrophosphatase activity
F	0016787	molecular_function	hydrolase activity
F	0016791	molecular_function	phosphatase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0030282	biological_process	bone mineralization
F	0031012	cellular_component	extracellular matrix
F	0031214	biological_process	biomineral tissue development
F	0031966	cellular_component	mitochondrial membrane
F	0032496	biological_process	response to lipopolysaccharide
F	0032868	biological_process	response to insulin
F	0033280	biological_process	response to vitamin D
F	0033883	molecular_function	pyridoxal phosphatase activity
F	0034516	biological_process	response to vitamin B6
F	0036005	biological_process	response to macrophage colony-stimulating factor
F	0042822	biological_process	pyridoxal phosphate metabolic process
F	0043262	molecular_function	ADP phosphatase activity
F	0046872	molecular_function	metal ion binding
F	0050187	molecular_function	phosphoamidase activity
F	0051384	biological_process	response to glucocorticoid
F	0052732	molecular_function	phosphoethanolamine phosphatase activity
F	0055062	biological_process	phosphate ion homeostasis
F	0055074	biological_process	calcium ion homeostasis
F	0065010	cellular_component	extracellular membrane-bounded organelle
F	0070062	cellular_component	extracellular exosome
F	0071529	biological_process	cementum mineralization
F	0098552	cellular_component	side of membrane
F	0120162	biological_process	positive regulation of cold-induced thermogenesis
F	0140651	biological_process	futile creatine cycle
F	0140928	biological_process	inhibition of non-skeletal tissue mineralization
F	1904383	biological_process	response to sodium phosphate
G	0001501	biological_process	skeletal system development
G	0001649	biological_process	osteoblast differentiation
G	0004035	molecular_function	alkaline phosphatase activity
G	0004427	molecular_function	inorganic diphosphate phosphatase activity
G	0005509	molecular_function	calcium ion binding
G	0005576	cellular_component	extracellular region
G	0005615	cellular_component	extracellular space
G	0005758	cellular_component	mitochondrial intermembrane space
G	0005886	cellular_component	plasma membrane
G	0016020	cellular_component	membrane
G	0016462	molecular_function	pyrophosphatase activity
G	0016787	molecular_function	hydrolase activity
G	0016791	molecular_function	phosphatase activity
G	0016887	molecular_function	ATP hydrolysis activity
G	0030282	biological_process	bone mineralization
G	0031012	cellular_component	extracellular matrix
G	0031214	biological_process	biomineral tissue development
G	0031966	cellular_component	mitochondrial membrane
G	0032496	biological_process	response to lipopolysaccharide
G	0032868	biological_process	response to insulin
G	0033280	biological_process	response to vitamin D
G	0033883	molecular_function	pyridoxal phosphatase activity
G	0034516	biological_process	response to vitamin B6
G	0036005	biological_process	response to macrophage colony-stimulating factor
G	0042822	biological_process	pyridoxal phosphate metabolic process
G	0043262	molecular_function	ADP phosphatase activity
G	0046872	molecular_function	metal ion binding
G	0050187	molecular_function	phosphoamidase activity
G	0051384	biological_process	response to glucocorticoid
G	0052732	molecular_function	phosphoethanolamine phosphatase activity
G	0055062	biological_process	phosphate ion homeostasis
G	0055074	biological_process	calcium ion homeostasis
G	0065010	cellular_component	extracellular membrane-bounded organelle
G	0070062	cellular_component	extracellular exosome
G	0071529	biological_process	cementum mineralization
G	0098552	cellular_component	side of membrane
G	0120162	biological_process	positive regulation of cold-induced thermogenesis
G	0140651	biological_process	futile creatine cycle
G	0140928	biological_process	inhibition of non-skeletal tissue mineralization
G	1904383	biological_process	response to sodium phosphate
H	0001501	biological_process	skeletal system development
H	0001649	biological_process	osteoblast differentiation
H	0004035	molecular_function	alkaline phosphatase activity
H	0004427	molecular_function	inorganic diphosphate phosphatase activity
H	0005509	molecular_function	calcium ion binding
H	0005576	cellular_component	extracellular region
H	0005615	cellular_component	extracellular space
H	0005758	cellular_component	mitochondrial intermembrane space
H	0005886	cellular_component	plasma membrane
H	0016020	cellular_component	membrane
H	0016462	molecular_function	pyrophosphatase activity
H	0016787	molecular_function	hydrolase activity
H	0016791	molecular_function	phosphatase activity
H	0016887	molecular_function	ATP hydrolysis activity
H	0030282	biological_process	bone mineralization
H	0031012	cellular_component	extracellular matrix
H	0031214	biological_process	biomineral tissue development
H	0031966	cellular_component	mitochondrial membrane
H	0032496	biological_process	response to lipopolysaccharide
H	0032868	biological_process	response to insulin
H	0033280	biological_process	response to vitamin D
H	0033883	molecular_function	pyridoxal phosphatase activity
H	0034516	biological_process	response to vitamin B6
H	0036005	biological_process	response to macrophage colony-stimulating factor
H	0042822	biological_process	pyridoxal phosphate metabolic process
H	0043262	molecular_function	ADP phosphatase activity
H	0046872	molecular_function	metal ion binding
H	0050187	molecular_function	phosphoamidase activity
H	0051384	biological_process	response to glucocorticoid
H	0052732	molecular_function	phosphoethanolamine phosphatase activity
H	0055062	biological_process	phosphate ion homeostasis
H	0055074	biological_process	calcium ion homeostasis
H	0065010	cellular_component	extracellular membrane-bounded organelle
H	0070062	cellular_component	extracellular exosome
H	0071529	biological_process	cementum mineralization
H	0098552	cellular_component	side of membrane
H	0120162	biological_process	positive regulation of cold-induced thermogenesis
H	0140651	biological_process	futile creatine cycle
H	0140928	biological_process	inhibition of non-skeletal tissue mineralization
H	1904383	biological_process	response to sodium phosphate

Functional Information from PROSITE/UniProt

site_id	PS00123
Number of Residues	9
Details	ALKALINE_PHOSPHATASE Alkaline phosphatase active site. VpDSAGTAT

Chain	Residue	Details
A	VAL107-THR115

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Phosphoserine intermediate","evidences":[{"source":"UniProtKB","id":"P05187","evidenceCode":"ECO:0000250"},{"source":"PROSITE-ProRule","id":"PRU10042","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P05187","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11395499","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P09242","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Lipidation: {"description":"GPI-anchor amidated serine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	32
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---