Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7TAP

Cryo-EM structure of archazolid A bound to yeast VO V-ATPase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
A	0000324	cellular_component	fungal-type vacuole
A	0000329	cellular_component	fungal-type vacuole membrane
A	0005515	molecular_function	protein binding
A	0005773	cellular_component	vacuole
A	0005774	cellular_component	vacuolar membrane
A	0006797	biological_process	polyphosphate metabolic process
A	0007035	biological_process	vacuolar acidification
A	0016020	cellular_component	membrane
A	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
A	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
A	0045121	cellular_component	membrane raft
A	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
A	0051117	molecular_function	ATPase binding
A	0065003	biological_process	protein-containing complex assembly
A	0071469	biological_process	cellular response to alkaline pH
A	0071474	biological_process	cellular hyperosmotic response
A	0080025	molecular_function	phosphatidylinositol-3,5-bisphosphate binding
A	1902600	biological_process	proton transmembrane transport
A	1903778	biological_process	protein localization to vacuolar membrane
B	0000139	cellular_component	Golgi membrane
B	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
B	0000329	cellular_component	fungal-type vacuole membrane
B	0005515	molecular_function	protein binding
B	0005773	cellular_component	vacuole
B	0005774	cellular_component	vacuolar membrane
B	0007034	biological_process	vacuolar transport
B	0007035	biological_process	vacuolar acidification
B	0016020	cellular_component	membrane
B	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
B	0033176	cellular_component	proton-transporting V-type ATPase complex
B	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
B	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
B	0048388	biological_process	endosomal lumen acidification
B	0061795	biological_process	Golgi lumen acidification
B	1902600	biological_process	proton transmembrane transport
C	0000139	cellular_component	Golgi membrane
C	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
C	0000329	cellular_component	fungal-type vacuole membrane
C	0005773	cellular_component	vacuole
C	0005774	cellular_component	vacuolar membrane
C	0007035	biological_process	vacuolar acidification
C	0008553	molecular_function	P-type proton-exporting transporter activity
C	0015078	molecular_function	proton transmembrane transporter activity
C	0016020	cellular_component	membrane
C	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
C	0033176	cellular_component	proton-transporting V-type ATPase complex
C	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
C	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
C	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
C	0048388	biological_process	endosomal lumen acidification
C	0061795	biological_process	Golgi lumen acidification
C	1902600	biological_process	proton transmembrane transport
D	0000139	cellular_component	Golgi membrane
D	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
D	0000324	cellular_component	fungal-type vacuole
D	0000329	cellular_component	fungal-type vacuole membrane
D	0005773	cellular_component	vacuole
D	0005774	cellular_component	vacuolar membrane
D	0007035	biological_process	vacuolar acidification
D	0015078	molecular_function	proton transmembrane transporter activity
D	0016020	cellular_component	membrane
D	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
D	0033176	cellular_component	proton-transporting V-type ATPase complex
D	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
D	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
D	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
D	0048388	biological_process	endosomal lumen acidification
D	0061795	biological_process	Golgi lumen acidification
D	0071944	cellular_component	cell periphery
D	1902600	biological_process	proton transmembrane transport
E	0000139	cellular_component	Golgi membrane
E	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
E	0000329	cellular_component	fungal-type vacuole membrane
E	0005773	cellular_component	vacuole
E	0005774	cellular_component	vacuolar membrane
E	0006623	biological_process	protein targeting to vacuole
E	0006878	biological_process	intracellular copper ion homeostasis
E	0006879	biological_process	intracellular iron ion homeostasis
E	0006897	biological_process	endocytosis
E	0007033	biological_process	vacuole organization
E	0007035	biological_process	vacuolar acidification
E	0015078	molecular_function	proton transmembrane transporter activity
E	0016020	cellular_component	membrane
E	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
E	0033176	cellular_component	proton-transporting V-type ATPase complex
E	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
E	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
E	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
E	0048388	biological_process	endosomal lumen acidification
E	0061795	biological_process	Golgi lumen acidification
E	1902600	biological_process	proton transmembrane transport
F	0000139	cellular_component	Golgi membrane
F	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
F	0000329	cellular_component	fungal-type vacuole membrane
F	0005773	cellular_component	vacuole
F	0005774	cellular_component	vacuolar membrane
F	0006623	biological_process	protein targeting to vacuole
F	0006878	biological_process	intracellular copper ion homeostasis
F	0006879	biological_process	intracellular iron ion homeostasis
F	0006897	biological_process	endocytosis
F	0007033	biological_process	vacuole organization
F	0007035	biological_process	vacuolar acidification
F	0015078	molecular_function	proton transmembrane transporter activity
F	0016020	cellular_component	membrane
F	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
F	0033176	cellular_component	proton-transporting V-type ATPase complex
F	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
F	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
F	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
F	0048388	biological_process	endosomal lumen acidification
F	0061795	biological_process	Golgi lumen acidification
F	1902600	biological_process	proton transmembrane transport
G	0000139	cellular_component	Golgi membrane
G	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
G	0000329	cellular_component	fungal-type vacuole membrane
G	0005773	cellular_component	vacuole
G	0005774	cellular_component	vacuolar membrane
G	0006623	biological_process	protein targeting to vacuole
G	0006878	biological_process	intracellular copper ion homeostasis
G	0006879	biological_process	intracellular iron ion homeostasis
G	0006897	biological_process	endocytosis
G	0007033	biological_process	vacuole organization
G	0007035	biological_process	vacuolar acidification
G	0015078	molecular_function	proton transmembrane transporter activity
G	0016020	cellular_component	membrane
G	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
G	0033176	cellular_component	proton-transporting V-type ATPase complex
G	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
G	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
G	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
G	0048388	biological_process	endosomal lumen acidification
G	0061795	biological_process	Golgi lumen acidification
G	1902600	biological_process	proton transmembrane transport
H	0000139	cellular_component	Golgi membrane
H	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
H	0000329	cellular_component	fungal-type vacuole membrane
H	0005773	cellular_component	vacuole
H	0005774	cellular_component	vacuolar membrane
H	0006623	biological_process	protein targeting to vacuole
H	0006878	biological_process	intracellular copper ion homeostasis
H	0006879	biological_process	intracellular iron ion homeostasis
H	0006897	biological_process	endocytosis
H	0007033	biological_process	vacuole organization
H	0007035	biological_process	vacuolar acidification
H	0015078	molecular_function	proton transmembrane transporter activity
H	0016020	cellular_component	membrane
H	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
H	0033176	cellular_component	proton-transporting V-type ATPase complex
H	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
H	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
H	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
H	0048388	biological_process	endosomal lumen acidification
H	0061795	biological_process	Golgi lumen acidification
H	1902600	biological_process	proton transmembrane transport
I	0000139	cellular_component	Golgi membrane
I	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
I	0000329	cellular_component	fungal-type vacuole membrane
I	0005773	cellular_component	vacuole
I	0005774	cellular_component	vacuolar membrane
I	0006623	biological_process	protein targeting to vacuole
I	0006878	biological_process	intracellular copper ion homeostasis
I	0006879	biological_process	intracellular iron ion homeostasis
I	0006897	biological_process	endocytosis
I	0007033	biological_process	vacuole organization
I	0007035	biological_process	vacuolar acidification
I	0015078	molecular_function	proton transmembrane transporter activity
I	0016020	cellular_component	membrane
I	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
I	0033176	cellular_component	proton-transporting V-type ATPase complex
I	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
I	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
I	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
I	0048388	biological_process	endosomal lumen acidification
I	0061795	biological_process	Golgi lumen acidification
I	1902600	biological_process	proton transmembrane transport
J	0000139	cellular_component	Golgi membrane
J	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
J	0000329	cellular_component	fungal-type vacuole membrane
J	0005773	cellular_component	vacuole
J	0005774	cellular_component	vacuolar membrane
J	0006623	biological_process	protein targeting to vacuole
J	0006878	biological_process	intracellular copper ion homeostasis
J	0006879	biological_process	intracellular iron ion homeostasis
J	0006897	biological_process	endocytosis
J	0007033	biological_process	vacuole organization
J	0007035	biological_process	vacuolar acidification
J	0015078	molecular_function	proton transmembrane transporter activity
J	0016020	cellular_component	membrane
J	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
J	0033176	cellular_component	proton-transporting V-type ATPase complex
J	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
J	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
J	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
J	0048388	biological_process	endosomal lumen acidification
J	0061795	biological_process	Golgi lumen acidification
J	1902600	biological_process	proton transmembrane transport
K	0000139	cellular_component	Golgi membrane
K	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
K	0000329	cellular_component	fungal-type vacuole membrane
K	0005773	cellular_component	vacuole
K	0005774	cellular_component	vacuolar membrane
K	0006623	biological_process	protein targeting to vacuole
K	0006878	biological_process	intracellular copper ion homeostasis
K	0006879	biological_process	intracellular iron ion homeostasis
K	0006897	biological_process	endocytosis
K	0007033	biological_process	vacuole organization
K	0007035	biological_process	vacuolar acidification
K	0015078	molecular_function	proton transmembrane transporter activity
K	0016020	cellular_component	membrane
K	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
K	0033176	cellular_component	proton-transporting V-type ATPase complex
K	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
K	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
K	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
K	0048388	biological_process	endosomal lumen acidification
K	0061795	biological_process	Golgi lumen acidification
K	1902600	biological_process	proton transmembrane transport
L	0000139	cellular_component	Golgi membrane
L	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
L	0000329	cellular_component	fungal-type vacuole membrane
L	0005773	cellular_component	vacuole
L	0005774	cellular_component	vacuolar membrane
L	0006623	biological_process	protein targeting to vacuole
L	0006878	biological_process	intracellular copper ion homeostasis
L	0006879	biological_process	intracellular iron ion homeostasis
L	0006897	biological_process	endocytosis
L	0007033	biological_process	vacuole organization
L	0007035	biological_process	vacuolar acidification
L	0015078	molecular_function	proton transmembrane transporter activity
L	0016020	cellular_component	membrane
L	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
L	0033176	cellular_component	proton-transporting V-type ATPase complex
L	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
L	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
L	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
L	0048388	biological_process	endosomal lumen acidification
L	0061795	biological_process	Golgi lumen acidification
L	1902600	biological_process	proton transmembrane transport
M	0000032	biological_process	cell wall mannoprotein biosynthetic process
M	0000139	cellular_component	Golgi membrane
M	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
M	0000329	cellular_component	fungal-type vacuole membrane
M	0005773	cellular_component	vacuole
M	0005774	cellular_component	vacuolar membrane
M	0007035	biological_process	vacuolar acidification
M	0016020	cellular_component	membrane
M	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
M	0033176	cellular_component	proton-transporting V-type ATPase complex
M	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
M	0042625	molecular_function	ATPase-coupled ion transmembrane transporter activity
M	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
M	0048388	biological_process	endosomal lumen acidification
M	0055085	biological_process	transmembrane transport
M	0061795	biological_process	Golgi lumen acidification
M	0065003	biological_process	protein-containing complex assembly
M	1902600	biological_process	proton transmembrane transport
O	0000139	cellular_component	Golgi membrane
O	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
O	0000329	cellular_component	fungal-type vacuole membrane
O	0003674	molecular_function	molecular_function
O	0004521	molecular_function	RNA endonuclease activity
O	0005575	cellular_component	cellular_component
O	0005773	cellular_component	vacuole
O	0005774	cellular_component	vacuolar membrane
O	0005783	cellular_component	endoplasmic reticulum
O	0005789	cellular_component	endoplasmic reticulum membrane
O	0007035	biological_process	vacuolar acidification
O	0008150	biological_process	biological_process
O	0016020	cellular_component	membrane
O	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
O	0033176	cellular_component	proton-transporting V-type ATPase complex
O	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
O	0048388	biological_process	endosomal lumen acidification
O	0061795	biological_process	Golgi lumen acidification
O	0090304	biological_process	nucleic acid metabolic process
O	1902600	biological_process	proton transmembrane transport

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	529
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
A	ALA2-GLU404
H	MET1-TYR8
H	CYS75-GLN90
H	ASN151-CYS160
I	MET1-TYR8
I	CYS75-GLN90
I	ASN151-CYS160
J	MET1-TYR8
J	CYS75-GLN90
J	ASN151-CYS160
K	MET1-TYR8
A	GLU443-ASP456
K	CYS75-GLN90
K	ASN151-CYS160
L	MET1-TYR8
L	CYS75-GLN90
L	ASN151-CYS160
A	SER555-PHE572
A	LYS657-THR719
A	SER805-SER840
F	ASN151-CYS160
G	MET1-TYR8
G	CYS75-GLN90
G	ASN151-CYS160

site_id	SWS_FT_FI2
Number of Residues	183
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
A	ILE405-PHE423
G	ILE54-VAL74
G	LEU91-GLY112
G	LEU125-LEU150
H	ALA9-GLY31
H	ILE54-VAL74
H	LEU91-GLY112
H	LEU125-LEU150
I	ALA9-GLY31
I	ILE54-VAL74
I	LEU91-GLY112
A	MET426-ASN442
I	LEU125-LEU150
J	ALA9-GLY31
J	ILE54-VAL74
J	LEU91-GLY112
J	LEU125-LEU150
K	ALA9-GLY31
K	ILE54-VAL74
K	LEU91-GLY112
K	LEU125-LEU150
L	ALA9-GLY31
A	MET457-THR486
L	ILE54-VAL74
L	LEU91-GLY112
L	LEU125-LEU150
A	TYR535-PHE554
A	ILE573-LYS593
A	LEU637-PHE656
A	ILE720-ALA744
A	GLY766-GLU804
G	ALA9-GLY31

site_id	SWS_FT_FI3
Number of Residues	110
Details	TOPO_DOM: Vacuolar => ECO:0000255

Chain	Residue	Details
A	GLY424-ASP425
I	ASP113-ARG124
J	THR32-ASN53
J	ASP113-ARG124
K	THR32-ASN53
K	ASP113-ARG124
L	THR32-ASN53
L	ASP113-ARG124
A	MET487-SER534
A	TRP594-PHE636
A	GLN745-VAL765
G	THR32-ASN53
G	ASP113-ARG124
H	THR32-ASN53
H	ASP113-ARG124
I	THR32-ASN53

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269\|Ref.6, ECO:0007744\|PubMed:22814378

Chain	Residue	Details
A	ALA2
F	GLU137
G	GLU137
H	GLU137
I	GLU137
J	GLU137
K	GLU137
L	GLU137

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)