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7T90

Cryo-EM structure of ACh-bound M2R-Go signaling complex in S2 state

Functional Information from GO Data
ChainGOidnamespacecontents
A0004930molecular_functionG protein-coupled receptor activity
A0005886cellular_componentplasma membrane
A0007186biological_processG protein-coupled receptor signaling pathway
A0016020cellular_componentmembrane
A0016907molecular_functionG protein-coupled acetylcholine receptor activity
B0003924molecular_functionGTPase activity
B0005515molecular_functionprotein binding
B0005525molecular_functionGTP binding
B0005737cellular_componentcytoplasm
B0005834cellular_componentheterotrimeric G-protein complex
B0005886cellular_componentplasma membrane
B0006904biological_processvesicle docking involved in exocytosis
B0006936biological_processmuscle contraction
B0007165biological_processsignal transduction
B0007186biological_processG protein-coupled receptor signaling pathway
B0007188biological_processadenylate cyclase-modulating G protein-coupled receptor signaling pathway
B0007212biological_processG protein-coupled dopamine receptor signaling pathway
B0007626biological_processlocomotory behavior
B0008016biological_processregulation of heart contraction
B0016020cellular_componentmembrane
B0019001molecular_functionguanyl nucleotide binding
B0030425cellular_componentdendrite
B0031683molecular_functionG-protein beta/gamma-subunit complex binding
B0031821molecular_functionG protein-coupled serotonin receptor binding
B0031852molecular_functionmu-type opioid receptor binding
B0044297cellular_componentcell body
B0046676biological_processnegative regulation of insulin secretion
B0046872molecular_functionmetal ion binding
B0051430molecular_functioncorticotropin-releasing hormone receptor 1 binding
C0001750cellular_componentphotoreceptor outer segment
C0003924molecular_functionGTPase activity
C0005515molecular_functionprotein binding
C0005737cellular_componentcytoplasm
C0005765cellular_componentlysosomal membrane
C0005829cellular_componentcytosol
C0005834cellular_componentheterotrimeric G-protein complex
C0005886cellular_componentplasma membrane
C0007165biological_processsignal transduction
C0007186biological_processG protein-coupled receptor signaling pathway
C0007191biological_processadenylate cyclase-activating dopamine receptor signaling pathway
C0007200biological_processphospholipase C-activating G protein-coupled receptor signaling pathway
C0007213biological_processG protein-coupled acetylcholine receptor signaling pathway
C0007265biological_processRas protein signal transduction
C0008283biological_processcell population proliferation
C0016020cellular_componentmembrane
C0030159molecular_functionsignaling receptor complex adaptor activity
C0044877molecular_functionprotein-containing complex binding
C0045202cellular_componentsynapse
C0050909biological_processsensory perception of taste
C0051020molecular_functionGTPase binding
C0060041biological_processretina development in camera-type eye
C0070062cellular_componentextracellular exosome
C0071380biological_processcellular response to prostaglandin E stimulus
C0071870biological_processcellular response to catecholamine stimulus
C0097381cellular_componentphotoreceptor disc membrane
C1903561cellular_componentextracellular vesicle
D0005515molecular_functionprotein binding
D0005834cellular_componentheterotrimeric G-protein complex
D0005886cellular_componentplasma membrane
D0007165biological_processsignal transduction
D0007186biological_processG protein-coupled receptor signaling pathway
D0007191biological_processadenylate cyclase-activating dopamine receptor signaling pathway
D0016020cellular_componentmembrane
D0031681molecular_functionG-protein beta-subunit binding
D0048144biological_processfibroblast proliferation
D0070062cellular_componentextracellular exosome
D0071380biological_processcellular response to prostaglandin E stimulus
D0071870biological_processcellular response to catecholamine stimulus
Functional Information from PROSITE/UniProt
site_idPS00237
Number of Residues17
DetailsG_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASVmNLLIISFDRYFcV
ChainResidueDetails
AALA109-VAL125

site_idPS00678
Number of Residues15
DetailsWD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS
ChainResidueDetails
CLEU70-SER84
CILE157-ILE171
CLEU285-ALA299

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsMOD_RES: N-acetylalanine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22814378
ChainResidueDetails
DALA2
BSER47
BLEU176
BTHR182
BASP201
BASN270
BALA326

site_idSWS_FT_FI2
Number of Residues1
DetailsMOD_RES: Cysteine methyl ester => ECO:0000250|UniProtKB:P63212
ChainResidueDetails
DCYS68
AASP120-MET139
ACYS443-ARG466

site_idSWS_FT_FI3
Number of Residues1
DetailsLIPID: S-geranylgeranyl cysteine => ECO:0000250|UniProtKB:P63212
ChainResidueDetails
DCYS68

site_idSWS_FT_FI4
Number of Residues1
DetailsMOD_RES: ADP-ribosylcysteine; by pertussis toxin => ECO:0000250
ChainResidueDetails
BCYS351
AGLN163-ALA184
ATHR411-PRO418

site_idSWS_FT_FI5
Number of Residues1
DetailsLIPID: N-myristoyl glycine => ECO:0000269|PubMed:25255805
ChainResidueDetails
BGLY2

site_idSWS_FT_FI6
Number of Residues1
DetailsLIPID: S-palmitoyl cysteine => ECO:0000250
ChainResidueDetails
BCYS3

site_idSWS_FT_FI7
Number of Residues24
DetailsTRANSMEM: Helical; Name=5
ChainResidueDetails
AALA185-ILE209

site_idSWS_FT_FI8
Number of Residues22
DetailsTRANSMEM: Helical; Name=6
ChainResidueDetails
ATHR388-ASN410

site_idSWS_FT_FI9
Number of Residues23
DetailsTRANSMEM: Helical; Name=7
ChainResidueDetails
AASN419-LEU442

site_idSWS_FT_FI10
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9ERZ4
ChainResidueDetails
ASER359

site_idSWS_FT_FI11
Number of Residues3
DetailsMOD_RES: Phosphothreonine => ECO:0000255
ChainResidueDetails
ATHR446
ATHR450
ATHR465

site_idSWS_FT_FI12
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASP6
AASP9

222926

PDB entries from 2024-07-24

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