7O6Y

Cryo-EM structure of respiratory complex I under turnover

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0003954	molecular_function	NADH dehydrogenase activity
1	0005739	cellular_component	mitochondrion
1	0005743	cellular_component	mitochondrial inner membrane
1	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
1	0009060	biological_process	aerobic respiration
1	0016020	cellular_component	membrane
1	0045271	cellular_component	respiratory chain complex I
1	1902600	biological_process	proton transmembrane transport
2	0005739	cellular_component	mitochondrion
2	0005743	cellular_component	mitochondrial inner membrane
2	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
2	1902600	biological_process	proton transmembrane transport
3	0005739	cellular_component	mitochondrion
3	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
3	0016020	cellular_component	membrane
3	0031966	cellular_component	mitochondrial membrane
3	0045271	cellular_component	respiratory chain complex I
3	1902600	biological_process	proton transmembrane transport
4	0003954	molecular_function	NADH dehydrogenase activity
4	0005739	cellular_component	mitochondrion
4	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
4	0009060	biological_process	aerobic respiration
4	0015990	biological_process	electron transport coupled proton transport
4	0016020	cellular_component	membrane
4	0031966	cellular_component	mitochondrial membrane
4	0042773	biological_process	ATP synthesis coupled electron transport
4	0045271	cellular_component	respiratory chain complex I
4	0048039	molecular_function	ubiquinone binding
5	0003954	molecular_function	NADH dehydrogenase activity
5	0005739	cellular_component	mitochondrion
5	0005743	cellular_component	mitochondrial inner membrane
5	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
5	0015990	biological_process	electron transport coupled proton transport
5	0042773	biological_process	ATP synthesis coupled electron transport
5	0045271	cellular_component	respiratory chain complex I
6	0005739	cellular_component	mitochondrion
6	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
6	0016020	cellular_component	membrane
6	0031966	cellular_component	mitochondrial membrane
6	1902600	biological_process	proton transmembrane transport
8	0005739	cellular_component	mitochondrion
8	0005743	cellular_component	mitochondrial inner membrane
8	0005758	cellular_component	mitochondrial intermembrane space
8	0016020	cellular_component	membrane
8	0045271	cellular_component	respiratory chain complex I
9	0005739	cellular_component	mitochondrion
9	0005743	cellular_component	mitochondrial inner membrane
9	0005758	cellular_component	mitochondrial intermembrane space
9	0032981	biological_process	mitochondrial respiratory chain complex I assembly
9	0045271	cellular_component	respiratory chain complex I
A	0003954	molecular_function	NADH dehydrogenase activity
A	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
A	0042773	biological_process	ATP synthesis coupled electron transport
A	0045271	cellular_component	respiratory chain complex I
A	0045333	biological_process	cellular respiration
A	0046872	molecular_function	metal ion binding
A	0051536	molecular_function	iron-sulfur cluster binding
A	0051539	molecular_function	4 iron, 4 sulfur cluster binding
A	1902600	biological_process	proton transmembrane transport
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
B	0010181	molecular_function	FMN binding
B	0045271	cellular_component	respiratory chain complex I
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
B	1902600	biological_process	proton transmembrane transport
C	0005739	cellular_component	mitochondrion
C	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
C	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
C	0045271	cellular_component	respiratory chain complex I
C	0048038	molecular_function	quinone binding
C	0051287	molecular_function	NAD binding
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0045271	cellular_component	respiratory chain complex I
E	0005739	cellular_component	mitochondrion
E	0044877	molecular_function	protein-containing complex binding
E	1901006	biological_process	ubiquinone-6 biosynthetic process
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0022904	biological_process	respiratory electron transport chain
F	0045271	cellular_component	respiratory chain complex I
G	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
G	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
G	0045271	cellular_component	respiratory chain complex I
G	1902600	biological_process	proton transmembrane transport
H	0016491	molecular_function	oxidoreductase activity
H	0045271	cellular_component	respiratory chain complex I
H	0046872	molecular_function	metal ion binding
H	0051537	molecular_function	2 iron, 2 sulfur cluster binding
I	0003954	molecular_function	NADH dehydrogenase activity
I	0005739	cellular_component	mitochondrion
I	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
I	0016020	cellular_component	membrane
I	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
I	0032981	biological_process	mitochondrial respiratory chain complex I assembly
I	0045271	cellular_component	respiratory chain complex I
I	0051539	molecular_function	4 iron, 4 sulfur cluster binding
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0005744	cellular_component	TIM23 mitochondrial import inner membrane translocase complex
J	0008320	molecular_function	protein transmembrane transporter activity
J	0030150	biological_process	protein import into mitochondrial matrix
J	0032981	biological_process	mitochondrial respiratory chain complex I assembly
K	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
K	0048038	molecular_function	quinone binding
K	0051536	molecular_function	iron-sulfur cluster binding
K	0051539	molecular_function	4 iron, 4 sulfur cluster binding
L	0005739	cellular_component	mitochondrion
L	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
L	0016020	cellular_component	membrane
L	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
L	0031966	cellular_component	mitochondrial membrane
L	0042773	biological_process	ATP synthesis coupled electron transport
L	0045271	cellular_component	respiratory chain complex I
L	1902600	biological_process	proton transmembrane transport
M	0005739	cellular_component	mitochondrion
M	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
M	0045271	cellular_component	respiratory chain complex I
O	0000035	molecular_function	acyl binding
O	0000036	molecular_function	acyl carrier activity
O	0005739	cellular_component	mitochondrion
O	0006633	biological_process	fatty acid biosynthetic process
O	0009107	biological_process	lipoate biosynthetic process
O	0016226	biological_process	iron-sulfur cluster assembly
O	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
O	1902494	cellular_component	catalytic complex
P	0005739	cellular_component	mitochondrion
P	0005743	cellular_component	mitochondrial inner membrane
P	0006979	biological_process	response to oxidative stress
P	0045271	cellular_component	respiratory chain complex I
Q	0000035	molecular_function	acyl binding
Q	0000036	molecular_function	acyl carrier activity
Q	0005739	cellular_component	mitochondrion
Q	0006633	biological_process	fatty acid biosynthetic process
Q	1902494	cellular_component	catalytic complex
R	0005739	cellular_component	mitochondrion
R	0005743	cellular_component	mitochondrial inner membrane
R	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
R	0045271	cellular_component	respiratory chain complex I
U	0005739	cellular_component	mitochondrion
U	0005743	cellular_component	mitochondrial inner membrane
U	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
U	0045271	cellular_component	respiratory chain complex I
W	0005739	cellular_component	mitochondrion
W	0005743	cellular_component	mitochondrial inner membrane
W	0016020	cellular_component	membrane
W	0045271	cellular_component	respiratory chain complex I
X	0016020	cellular_component	membrane
X	0045271	cellular_component	respiratory chain complex I
Y	0005739	cellular_component	mitochondrion
Y	0005743	cellular_component	mitochondrial inner membrane
Y	0022900	biological_process	electron transport chain
Y	0045271	cellular_component	respiratory chain complex I
a	0005739	cellular_component	mitochondrion
a	0016020	cellular_component	membrane
a	0045271	cellular_component	respiratory chain complex I
c	0005739	cellular_component	mitochondrion
c	0005743	cellular_component	mitochondrial inner membrane
c	0022900	biological_process	electron transport chain
c	0032981	biological_process	mitochondrial respiratory chain complex I assembly
c	0045271	cellular_component	respiratory chain complex I
d	0005739	cellular_component	mitochondrion
d	0005743	cellular_component	mitochondrial inner membrane
d	0045271	cellular_component	respiratory chain complex I
f	0005739	cellular_component	mitochondrion
f	0005743	cellular_component	mitochondrial inner membrane
f	0045271	cellular_component	respiratory chain complex I
g	0016020	cellular_component	membrane
h	0005739	cellular_component	mitochondrion
h	0005743	cellular_component	mitochondrial inner membrane
h	0006979	biological_process	response to oxidative stress
h	0016020	cellular_component	membrane
h	0032981	biological_process	mitochondrial respiratory chain complex I assembly
h	0045271	cellular_component	respiratory chain complex I
i	0016020	cellular_component	membrane
j	0016020	cellular_component	membrane

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGLDSLDVVEVVMAI

Chain	Residue	Details
Q	ASP84-ILE99
O	ASP61-ILE76

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site_id	PS00133
Number of Residues	11
Details	CARBOXYPEPT_ZN_2 Zinc carboxypeptidases, zinc-binding region 2 signature. HSIAGLFiFPW

Chain	Residue	Details
X	HIS145-TRP155

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site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiACKlCEaICP

Chain	Residue	Details
I	CYS130-PRO141
I	CYS169-PRO180

---

site_id	PS00535
Number of Residues	12
Details	COMPLEX1_49K Respiratory chain NADH dehydrogenase 49 Kd subunit signature. LHRGtEKLiEyK

Chain	Residue	Details
C	LEU119-LYS130

---

site_id	PS00542
Number of Residues	22
Details	COMPLEX1_30K Respiratory chain NADH dehydrogenase 30 Kd subunit signature. EREayDMYgvffegHpdlRrIM

Chain	Residue	Details
G	GLU179-MET200

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site_id	PS00641
Number of Residues	18
Details	COMPLEX1_75K_1 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. PryCYhdklaiaGnCRmC

Chain	Residue	Details
A	PRO66-CYS83

---

site_id	PS00642
Number of Residues	13
Details	COMPLEX1_75K_2 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. CPvCDqGGeCdLQ

Chain	Residue	Details
A	CYS133-GLN145

---

site_id	PS00643
Number of Residues	11
Details	COMPLEX1_75K_3 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. RCIhCtRCVrF

Chain	Residue	Details
A	ARG182-PHE192

---

site_id	PS00644
Number of Residues	16
Details	COMPLEX1_51K_1 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. GMGAYVCGEETSLIES

Chain	Residue	Details
B	GLY202-SER217

---

site_id	PS00645
Number of Residues	12
Details	COMPLEX1_51K_2 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. ESCGqCtPCReG

Chain	Residue	Details
B	GLU379-GLY390

---

site_id	PS00667
Number of Residues	16
Details	COMPLEX1_ND1_1 Respiratory-chain NADH dehydrogenase subunit 1 signature 1. GLLQaFaDAVKLLlKE

Chain	Residue	Details
1	GLY46-GLU61

---

site_id	PS00668
Number of Residues	14
Details	COMPLEX1_ND1_2 Respiratory-chain NADH dehydrogenase subunit 1 signature 2. PFDLTESEseLVa.G

Chain	Residue	Details
1	PRO201-GLY214

---

site_id	PS01099
Number of Residues	19
Details	COMPLEX1_24K Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. DnlFTlseveCLGaCvnAP

Chain	Residue	Details
H	ASP158-PRO176

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site_id	PS01150
Number of Residues	17
Details	COMPLEX1_20K Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. GcDRIVPVDVYvPgCPP

Chain	Residue	Details
K	GLY166-PRO182

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
L	ASN21-LEU41
5	ILE304-LEU322
5	LEU332-LEU354
5	LEU370-LEU390
5	VAL413-LEU433
5	ILE456-LEU476
5	ILE516-PHE536
5	LEU629-ILE649
L	PHE55-VAL75
6	ILE54-ILE74
6	LEU94-ASN114
6	ALA159-ILE179
5	PHE180-ILE200
5	ALA204-MET224
5	THR244-LEU264
5	VAL276-ILE296

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