7MEX

Structure of yeast Ubr1 in complex with Ubc2 and N-degron

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0000209	biological_process	protein polyubiquitination
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006513	biological_process	protein monoubiquitination
A	0008270	molecular_function	zinc ion binding
A	0008540	cellular_component	proteasome regulatory particle, base subcomplex
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0034620	biological_process	cellular response to unfolded protein
A	0036503	biological_process	ERAD pathway
A	0046872	molecular_function	metal ion binding
A	0061630	molecular_function	ubiquitin protein ligase activity
A	0071596	biological_process	ubiquitin-dependent protein catabolic process via the N-end rule pathway
A	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
A	0072671	biological_process	mitochondria-associated ubiquitin-dependent protein catabolic process
A	0090089	biological_process	regulation of dipeptide transport
A	0120174	biological_process	stress-induced homeostatically regulated protein degradation pathway
A	1904855	molecular_function	proteasome regulatory particle binding
A	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
A	1990303	cellular_component	UBR1-RAD6 ubiquitin ligase complex

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. FHPNVyan.GeICLdiL

Chain	Residue	Details
B	PHE77-LEU92

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site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
C	LYS27-ASP52

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	73
Details	ZN_FING: UBR-type => ECO:0000255|PROSITE-ProRule:PRU00508

Chain	Residue	Details
A	ARG121-GLN194
C	ARG72

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site_id	SWS_FT_FI2
Number of Residues	104
Details	ZN_FING: RING-type; atypical => ECO:0000305|PubMed:34789879

Chain	Residue	Details
A	CYS1220-GLN1324

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site_id	SWS_FT_FI3
Number of Residues	27
Details	BINDING: BINDING => ECO:0000269|PubMed:34789879, ECO:0007744|PDB:7MEX, ECO:0007744|PDB:7MEY

Chain	Residue	Details
A	HIS118
A	CYS175
A	CYS177
A	CYS189
A	ASP952
A	CYS1220
A	CYS1223
A	CYS1295
A	HIS1297
A	HIS1300
A	CYS1303
A	CYS123
A	CYS1320
A	CYS1323
A	CYS1703
A	CYS1706
A	HIS1722
A	CYS1727
A	HIS1763
A	ASP1775
A	CYS136
A	CYS139
A	CYS148
A	CYS151
A	HIS157
A	HIS160
A	HIS161

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER296

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER300

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site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER1938

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site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
C	CYS76

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site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
C	LYS11
C	LYS48

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site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
C	LYS27

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site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
C	LYS29

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site_id	SWS_FT_FI11
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
C	LYS33

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site_id	SWS_FT_FI12
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
C	LYS63

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 939

Chain	Residue	Details
B	CYS88	nucleophile

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