Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7M32

Dihydropyrimidine Dehydrogenase (DPD) C671A Mutant Soaked with Uracil and NADPH Anaerobically

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002058	molecular_function	uracil binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006210	biological_process	thymine catabolic process
A	0006212	biological_process	uracil catabolic process
A	0010181	molecular_function	FMN binding
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0017113	molecular_function	dihydropyrimidine dehydrogenase (NADP+) activity
A	0019483	biological_process	beta-alanine biosynthetic process
A	0042803	molecular_function	protein homodimerization activity
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0050661	molecular_function	NADP binding
A	0051536	molecular_function	iron-sulfur cluster binding
B	0002058	molecular_function	uracil binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006210	biological_process	thymine catabolic process
B	0006212	biological_process	uracil catabolic process
B	0010181	molecular_function	FMN binding
B	0016491	molecular_function	oxidoreductase activity
B	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
B	0017113	molecular_function	dihydropyrimidine dehydrogenase (NADP+) activity
B	0019483	biological_process	beta-alanine biosynthetic process
B	0042803	molecular_function	protein homodimerization activity
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0050661	molecular_function	NADP binding
B	0051536	molecular_function	iron-sulfur cluster binding
C	0002058	molecular_function	uracil binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006210	biological_process	thymine catabolic process
C	0006212	biological_process	uracil catabolic process
C	0010181	molecular_function	FMN binding
C	0016491	molecular_function	oxidoreductase activity
C	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
C	0017113	molecular_function	dihydropyrimidine dehydrogenase (NADP+) activity
C	0019483	biological_process	beta-alanine biosynthetic process
C	0042803	molecular_function	protein homodimerization activity
C	0050660	molecular_function	flavin adenine dinucleotide binding
C	0050661	molecular_function	NADP binding
C	0051536	molecular_function	iron-sulfur cluster binding
D	0002058	molecular_function	uracil binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006210	biological_process	thymine catabolic process
D	0006212	biological_process	uracil catabolic process
D	0010181	molecular_function	FMN binding
D	0016491	molecular_function	oxidoreductase activity
D	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
D	0017113	molecular_function	dihydropyrimidine dehydrogenase (NADP+) activity
D	0019483	biological_process	beta-alanine biosynthetic process
D	0042803	molecular_function	protein homodimerization activity
D	0050660	molecular_function	flavin adenine dinucleotide binding
D	0050661	molecular_function	NADP binding
D	0051536	molecular_function	iron-sulfur cluster binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	124
Details	Domain: {"description":"4Fe-4S ferredoxin-type 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00711","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	128
Details	Domain: {"description":"4Fe-4S ferredoxin-type 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00711","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	116
Details	Domain: {"description":"4Fe-4S ferredoxin-type 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00711","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"11796730","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	84
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11179210","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11796730","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GT8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GTE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GTH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1H7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1H7X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M31","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F5W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F61","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F6N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8F61","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11179210","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11796730","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GTE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GTH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1H7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1H7X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M31","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F5W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F61","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F6N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8F6N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GT8","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11179210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1H7X","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11179210","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11796730","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GT8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GTH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1H7X","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7LJT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M31","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M31","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11796730","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GT8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GTH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F5W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F61","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F5W","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11796730","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11179210","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1GTE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GTH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1H7X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8F5W","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33755421","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36681231","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7LJU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F5W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8F61","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34032117","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7M32","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q12882","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 800

Chain	Residue	Details
A	LYS574	electrostatic stabiliser
A	ALA671	proton acceptor, proton donor
A	LYS709	electrostatic stabiliser

site_id	MCSA2
Number of Residues	3
Details	M-CSA 800

Chain	Residue	Details
B	LYS574	electrostatic stabiliser
B	ALA671	proton acceptor, proton donor
B	LYS709	electrostatic stabiliser

site_id	MCSA3
Number of Residues	3
Details	M-CSA 800

Chain	Residue	Details
C	LYS574	electrostatic stabiliser
C	ALA671	proton acceptor, proton donor
C	LYS709	electrostatic stabiliser

site_id	MCSA4
Number of Residues	3
Details	M-CSA 800

Chain	Residue	Details
D	LYS574	electrostatic stabiliser
D	ALA671	proton acceptor, proton donor
D	LYS709	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)