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7M32

Dihydropyrimidine Dehydrogenase (DPD) C671A Mutant Soaked with Uracil and NADPH Anaerobically

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0002058molecular_functionuracil binding
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0006210biological_processthymine catabolic process
A0006212biological_processuracil catabolic process
A0006214biological_processthymidine catabolic process
A0010181molecular_functionFMN binding
A0016491molecular_functionoxidoreductase activity
A0016627molecular_functionoxidoreductase activity, acting on the CH-CH group of donors
A0017113molecular_functiondihydropyrimidine dehydrogenase (NADP+) activity
A0019483biological_processbeta-alanine biosynthetic process
A0042803molecular_functionprotein homodimerization activity
A0046872molecular_functionmetal ion binding
A0050660molecular_functionflavin adenine dinucleotide binding
A0050661molecular_functionNADP binding
A0051536molecular_functioniron-sulfur cluster binding
A0051539molecular_function4 iron, 4 sulfur cluster binding
B0000166molecular_functionnucleotide binding
B0002058molecular_functionuracil binding
B0005737cellular_componentcytoplasm
B0005829cellular_componentcytosol
B0006210biological_processthymine catabolic process
B0006212biological_processuracil catabolic process
B0006214biological_processthymidine catabolic process
B0010181molecular_functionFMN binding
B0016491molecular_functionoxidoreductase activity
B0016627molecular_functionoxidoreductase activity, acting on the CH-CH group of donors
B0017113molecular_functiondihydropyrimidine dehydrogenase (NADP+) activity
B0019483biological_processbeta-alanine biosynthetic process
B0042803molecular_functionprotein homodimerization activity
B0046872molecular_functionmetal ion binding
B0050660molecular_functionflavin adenine dinucleotide binding
B0050661molecular_functionNADP binding
B0051536molecular_functioniron-sulfur cluster binding
B0051539molecular_function4 iron, 4 sulfur cluster binding
C0000166molecular_functionnucleotide binding
C0002058molecular_functionuracil binding
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0006210biological_processthymine catabolic process
C0006212biological_processuracil catabolic process
C0006214biological_processthymidine catabolic process
C0010181molecular_functionFMN binding
C0016491molecular_functionoxidoreductase activity
C0016627molecular_functionoxidoreductase activity, acting on the CH-CH group of donors
C0017113molecular_functiondihydropyrimidine dehydrogenase (NADP+) activity
C0019483biological_processbeta-alanine biosynthetic process
C0042803molecular_functionprotein homodimerization activity
C0046872molecular_functionmetal ion binding
C0050660molecular_functionflavin adenine dinucleotide binding
C0050661molecular_functionNADP binding
C0051536molecular_functioniron-sulfur cluster binding
C0051539molecular_function4 iron, 4 sulfur cluster binding
D0000166molecular_functionnucleotide binding
D0002058molecular_functionuracil binding
D0005737cellular_componentcytoplasm
D0005829cellular_componentcytosol
D0006210biological_processthymine catabolic process
D0006212biological_processuracil catabolic process
D0006214biological_processthymidine catabolic process
D0010181molecular_functionFMN binding
D0016491molecular_functionoxidoreductase activity
D0016627molecular_functionoxidoreductase activity, acting on the CH-CH group of donors
D0017113molecular_functiondihydropyrimidine dehydrogenase (NADP+) activity
D0019483biological_processbeta-alanine biosynthetic process
D0042803molecular_functionprotein homodimerization activity
D0046872molecular_functionmetal ion binding
D0050660molecular_functionflavin adenine dinucleotide binding
D0050661molecular_functionNADP binding
D0051536molecular_functioniron-sulfur cluster binding
D0051539molecular_function4 iron, 4 sulfur cluster binding
Functional Information from PROSITE/UniProt
site_idPS00198
Number of Residues12
Details4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CtGCTlCLsVCP
ChainResidueDetails
ACYS986-PRO997

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: Proton acceptor => ECO:0000269|PubMed:11796730
ChainResidueDetails
AALA671
BALA671
CALA671
DALA671

site_idSWS_FT_FI2
Number of Residues76
DetailsBINDING:
ChainResidueDetails
ACYS989
ACYS992
ACYS996
BCYS79
BCYS82
BCYS87
BCYS91
BCYS130
BCYS136
BCYS140
BGLN156
BASN609
BASN668
BASN736
BCYS953
BCYS956
BCYS959
BCYS963
BCYS986
BCYS989
BCYS992
BCYS996
CCYS79
CCYS82
CCYS87
CCYS91
CCYS130
CCYS136
CCYS140
CGLN156
CASN609
CASN668
CASN736
CCYS953
CCYS956
CCYS959
CCYS963
CCYS986
CCYS989
CCYS992
CCYS996
DCYS79
DCYS82
DCYS87
DCYS91
DCYS130
DCYS136
DCYS140
DGLN156
DASN609
DASN668
DASN736
DCYS953
DCYS956
DCYS959
DCYS963
DCYS986
DCYS989
DCYS992
DCYS996
ACYS79
ACYS82
ACYS87
ACYS91
ACYS130
ACYS136
ACYS140
AGLN156
AASN609
AASN668
AASN736
ACYS953
ACYS956
ACYS959
ACYS963
ACYS986

site_idSWS_FT_FI3
Number of Residues68
DetailsBINDING: BINDING => ECO:0000269|PubMed:11796730
ChainResidueDetails
ALYS574
ALYS709
AGLY767
ATHR793
ACYS816
BVAL129
BGLY194
BGLU218
BARG235
BLEU261
BALA340
BARG364
BARG371
BALA437
BGLY480
BASP481
BSER550
BLYS574
BLYS709
BGLY767
BTHR793
BCYS816
CVAL129
CGLY194
CGLU218
CARG235
CLEU261
CALA340
CARG364
CARG371
CALA437
CGLY480
CASP481
CSER550
CLYS574
CLYS709
CGLY767
CTHR793
CCYS816
DVAL129
DGLY194
DGLU218
DARG235
DLEU261
DALA340
DARG364
DARG371
DALA437
DGLY480
DASP481
DSER550
DLYS574
DLYS709
DGLY767
DTHR793
DCYS816
AVAL129
AGLY194
AGLU218
AARG235
ALEU261
AALA340
AARG364
AARG371
AALA437
AGLY480
AASP481
ASER550

site_idSWS_FT_FI4
Number of Residues4
DetailsMOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q12882
ChainResidueDetails
BLYS384
CLYS384
DLYS384
ALYS384

Catalytic Information from CSA
site_idMCSA1
Number of Residues3
DetailsM-CSA 800
ChainResidueDetails
ALYS574electrostatic stabiliser
AALA671proton acceptor, proton donor
ALYS709electrostatic stabiliser

site_idMCSA2
Number of Residues3
DetailsM-CSA 800
ChainResidueDetails
BLYS574electrostatic stabiliser
BALA671proton acceptor, proton donor
BLYS709electrostatic stabiliser

site_idMCSA3
Number of Residues3
DetailsM-CSA 800
ChainResidueDetails
CLYS574electrostatic stabiliser
CALA671proton acceptor, proton donor
CLYS709electrostatic stabiliser

site_idMCSA4
Number of Residues3
DetailsM-CSA 800
ChainResidueDetails
DLYS574electrostatic stabiliser
DALA671proton acceptor, proton donor
DLYS709electrostatic stabiliser

221051

PDB entries from 2024-06-12

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